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The map label for this gene is caiD-2 [H]

Identifier: 116328281

GI number: 116328281

Start: 1891902

End: 1892669

Strand: Direct

Name: caiD-2 [H]

Synonym: LBL_1614

Alternate gene names: 116328281

Gene position: 1891902-1892669 (Clockwise)

Preceding gene: 116328280

Following gene: 116328285

Centisome position: 52.34

GC content: 40.36

Gene sequence:

>768_bases
ATGCTTTCCGAAATTAGAAACAAACATATTCTTGAACTATACATAGAAACCGACGAAGTAAATTCCTTAAACGGAGATTT
TTTCAAAACGATTTCCGCAAAGTTAGACGCAATTAACAACGATCCGACAGTTAAAGCCGTCATATTAACTTCTAAAAACG
AAAAGTTTTTCTCGAACGGTTTTAATCCGGAAATTTTTGTCGGAAAGACCTCGGAACAAATTCGAGACGTTATGCATCTC
GCCTTGGATACAGCTTCCAAATATCTTTTCTTAGAAAGACCTGTGATTTGTGCTATGAACGGGCATGCTATGGGATTGGG
AGCGGTACTTGCGATTTTTTCTGATTATAGAATCATGATCGAAAAAAAAGGAAGGATCGGTTTTCCCGAATCTCAGATCG
GAATCAACTTTCCCGCGGTTCCGGGTTTTATGCTCAAAGAAATCGTAGGGATCACAAAAGCTCGGGATCTTTTATATTCA
GGAAAAGGGTTAAAAGCAGAGGAAGCATTAGAAATCGGTCTCATCGACGAAATTTCTTCTTCCTCGGAAGACTTGATGGC
GCGCGCTCGCAAATACTGTGATCAGTTTAAGGATATGGCGATGGGTTCTGTGATTGGAATTAAAGTATCTTTGCGCGATC
CGATTCGATCTTTTGCGGAACACAATTCGGGAAGAGACGTCAAACTTATTTCGGAAGCGGTATTTTCCAAAAACGGTCAA
GAAGGTATGACATCCATCTTGGAAAGAAGACGACCTATATTCCTGTAG

Upstream 100 bases:

>100_bases
TCCTTCATCAATTTGGAAGAATCCAAGATAGAACCGCTGAATTCTTTTTTTCGGAACTCGGGAAAAAATTCGAATTCAAT
TGAAAACAATAGGAGCGAAG

Downstream 100 bases:

>100_bases
ATTTTAATTTAGAAAAGAAGAATGGAGCAAGGCCTATAACCTCAAAAAATTGTCTAGAGTCCATCTCAAATCCCGTCGAG
CGCCAATAGAAGCGAGACGT

Product: enoyl-CoA hydratase/carnithine racemase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLSEIRNKHILELYIETDEVNSLNGDFFKTISAKLDAINNDPTVKAVILTSKNEKFFSNGFNPEIFVGKTSEQIRDVMHL
ALDTASKYLFLERPVICAMNGHAMGLGAVLAIFSDYRIMIEKKGRIGFPESQIGINFPAVPGFMLKEIVGITKARDLLYS
GKGLKAEEALEIGLIDEISSSSEDLMARARKYCDQFKDMAMGSVIGIKVSLRDPIRSFAEHNSGRDVKLISEAVFSKNGQ
EGMTSILERRRPIFL

Sequences:

>Translated_255_residues
MLSEIRNKHILELYIETDEVNSLNGDFFKTISAKLDAINNDPTVKAVILTSKNEKFFSNGFNPEIFVGKTSEQIRDVMHL
ALDTASKYLFLERPVICAMNGHAMGLGAVLAIFSDYRIMIEKKGRIGFPESQIGINFPAVPGFMLKEIVGITKARDLLYS
GKGLKAEEALEIGLIDEISSSSEDLMARARKYCDQFKDMAMGSVIGIKVSLRDPIRSFAEHNSGRDVKLISEAVFSKNGQ
EGMTSILERRRPIFL
>Mature_255_residues
MLSEIRNKHILELYIETDEVNSLNGDFFKTISAKLDAINNDPTVKAVILTSKNEKFFSNGFNPEIFVGKTSEQIRDVMHL
ALDTASKYLFLERPVICAMNGHAMGLGAVLAIFSDYRIMIEKKGRIGFPESQIGINFPAVPGFMLKEIVGITKARDLLYS
GKGLKAEEALEIGLIDEISSSSEDLMARARKYCDQFKDMAMGSVIGIKVSLRDPIRSFAEHNSGRDVKLISEAVFSKNGQ
EGMTSILERRRPIFL

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI68989263, Length=232, Percent_Identity=25.8620689655172, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI116235487, Length=250, Percent_Identity=22.8, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI221142681, Length=254, Percent_Identity=25.1968503937008, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=24.7104247104247, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790281, Length=183, Percent_Identity=27.8688524590164, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788682, Length=179, Percent_Identity=24.0223463687151, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17558304, Length=174, Percent_Identity=28.1609195402299, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=159, Percent_Identity=28.3018867924528, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=187, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=27.7551020408163, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=27.7551020408163, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921018, Length=197, Percent_Identity=28.9340101522843, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45550169, Length=247, Percent_Identity=25.1012145748988, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24583165, Length=197, Percent_Identity=26.9035532994924, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28417; Mature: 28417

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSEIRNKHILELYIETDEVNSLNGDFFKTISAKLDAINNDPTVKAVILTSKNEKFFSNG
CCCHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHCC
FNPEIFVGKTSEQIRDVMHLALDTASKYLFLERPVICAMNGHAMGLGAVLAIFSDYRIMI
CCCEEEEECCHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCHHHHHHHHHHHHCCEEEE
EKKGRIGFPESQIGINFPAVPGFMLKEIVGITKARDLLYSGKGLKAEEALEIGLIDEISS
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCHHHHCC
SSEDLMARARKYCDQFKDMAMGSVIGIKVSLRDPIRSFAEHNSGRDVKLISEAVFSKNGQ
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHCCCCH
EGMTSILERRRPIFL
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MLSEIRNKHILELYIETDEVNSLNGDFFKTISAKLDAINNDPTVKAVILTSKNEKFFSNG
CCCHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHCC
FNPEIFVGKTSEQIRDVMHLALDTASKYLFLERPVICAMNGHAMGLGAVLAIFSDYRIMI
CCCEEEEECCHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCHHHHHHHHHHHHCCEEEE
EKKGRIGFPESQIGINFPAVPGFMLKEIVGITKARDLLYSGKGLKAEEALEIGLIDEISS
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCHHHHCC
SSEDLMARARKYCDQFKDMAMGSVIGIKVSLRDPIRSFAEHNSGRDVKLISEAVFSKNGQ
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHCCCCH
EGMTSILERRRPIFL
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]