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The map label for this gene is pmbA [C]

Identifier: 116328247

GI number: 116328247

Start: 1837982

End: 1839316

Strand: Direct

Name: pmbA [C]

Synonym: LBL_1574

Alternate gene names: 116328247

Gene position: 1837982-1839316 (Clockwise)

Preceding gene: 116328246

Following gene: 116328248

Centisome position: 50.85

GC content: 42.62

Gene sequence:

>1335_bases
ATGAATTTGGATAGTTCCGTTGAATACGTATTAGATATTTGTAAAAAGAAAAGATTGGATCAATACGATCTCGTTGGTTC
CGAGTCCAAAGAGGTGGGAATCGAGCTCTTTCGAAAAAGAGTGAGTAATACCGAACTTTCCAATTCCAGAGGGATTGGGA
TCCGTCTTATCCAGTCCAGAAAGCCGGGTTATTCGTACAGTGAAAAATTATCCGAAGAAGCATTGTCTCAGATGGTGGAG
GACGCGCTTGCACAATCTAAAATTTCGGATCCGCTTGATATCGACCTTCCGGGACCGTGCGAACTTCCCAAAATCAATAT
TCGTTCTTACGAGGAATCTTTGGAATCTCTCGGTTTTGAGTGGTTGAAATCCACGGGTGAGAAGTTGGATGATCTTGCTT
GGTCGGTCGGAGGTAAAGTCGAGAATGTTCCATATTCTTATGCGGGTAAAACTTGGAGCCGATTCATATTAGCAAATTCG
AATGGACTTTATCATAGCGAAAAATCGAACCTGATTTCGGCGGGAGTCGCGTTGGTCGCGACCGACGGAAAGACCAAAAA
AATGGGGGGATATACACGTTCCGGTTTGGATTTAGATCGTATTGATCCTAAGTTGATCGTGTCCACGGCGGCGGAACGTT
CTTTGGCTTTTTTGGGAGCAAAACCTGTAAAAAGTGGTTTGTACAATATCGTTCTTTCTAATCGAATCACTCCTCAAATT
TTTGGAATGTTTTCTTCTCCGTTCTCGGCTGATAGTGTACAAAAAGGTTTGTCTAGACTGGAAGGTAAAATCGGTTCACA
AATCGCATCACAAAATTGGAACGTGTTTTGTGATCCGCATATTTCGGATTATCCAGGTTCGAGACTTCTGGATGCGGAGG
GGGTTTTAACGAGTAAAAAAGCGGTAATCGAGAATGGAATTCTGCAAACTTATCTCTATAATCTGGAATCCGCTAAAAAG
GAGGGAGTTGTTCCTACCGGAAATGCGGTTCGCTCATATGGAGGTAGGGTCGGCGCCTCGTTTAACAATTACGTTGTACC
TAAGGGAGATAAAACTCTCGAAGAATTATTGAGTGGCTTTCCGGAATGTATTTACGTTTTAAAGCTCGAAGGAGGTTCGG
GATGTAGCGCGGTTTCCGGAGAAATCTCGATCGGTGTACAAGGGATTTATTATAAAAATGGAATCCCCGTTCATCCAGTG
GATAGCATAACGATGAATCTGAATTTTTTTGATCTTCTTTTTAGAATCGAGGGAATCTCGAACGAATACAATGATTCCTA
TTCTTCCATCAAGGTTCCGGATATTCTCATTAGGGAAGCAAACATTGCGGGTTGA

Upstream 100 bases:

>100_bases
AACTTGCTGCCGGAATGTGCGGTGCGGCTTCTGGCTCGGTTCCGGTCACAGTGGGTCAACCTTCCTTGAAAGTGGACGAG
ATTCTCGTGGGAGGTCGTTC

Downstream 100 bases:

>100_bases
GTTTTCGCTTGCTTCTCTTTTTGAATTAGGGTTTTTTGTTTGAAACAAACTTTTCTATTTCGGGAGAAACCTTTTGAACT
TTTGGATCCATCTTTTACAA

Product: Zn-dependent protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MNLDSSVEYVLDICKKKRLDQYDLVGSESKEVGIELFRKRVSNTELSNSRGIGIRLIQSRKPGYSYSEKLSEEALSQMVE
DALAQSKISDPLDIDLPGPCELPKINIRSYEESLESLGFEWLKSTGEKLDDLAWSVGGKVENVPYSYAGKTWSRFILANS
NGLYHSEKSNLISAGVALVATDGKTKKMGGYTRSGLDLDRIDPKLIVSTAAERSLAFLGAKPVKSGLYNIVLSNRITPQI
FGMFSSPFSADSVQKGLSRLEGKIGSQIASQNWNVFCDPHISDYPGSRLLDAEGVLTSKKAVIENGILQTYLYNLESAKK
EGVVPTGNAVRSYGGRVGASFNNYVVPKGDKTLEELLSGFPECIYVLKLEGGSGCSAVSGEISIGVQGIYYKNGIPVHPV
DSITMNLNFFDLLFRIEGISNEYNDSYSSIKVPDILIREANIAG

Sequences:

>Translated_444_residues
MNLDSSVEYVLDICKKKRLDQYDLVGSESKEVGIELFRKRVSNTELSNSRGIGIRLIQSRKPGYSYSEKLSEEALSQMVE
DALAQSKISDPLDIDLPGPCELPKINIRSYEESLESLGFEWLKSTGEKLDDLAWSVGGKVENVPYSYAGKTWSRFILANS
NGLYHSEKSNLISAGVALVATDGKTKKMGGYTRSGLDLDRIDPKLIVSTAAERSLAFLGAKPVKSGLYNIVLSNRITPQI
FGMFSSPFSADSVQKGLSRLEGKIGSQIASQNWNVFCDPHISDYPGSRLLDAEGVLTSKKAVIENGILQTYLYNLESAKK
EGVVPTGNAVRSYGGRVGASFNNYVVPKGDKTLEELLSGFPECIYVLKLEGGSGCSAVSGEISIGVQGIYYKNGIPVHPV
DSITMNLNFFDLLFRIEGISNEYNDSYSSIKVPDILIREANIAG
>Mature_444_residues
MNLDSSVEYVLDICKKKRLDQYDLVGSESKEVGIELFRKRVSNTELSNSRGIGIRLIQSRKPGYSYSEKLSEEALSQMVE
DALAQSKISDPLDIDLPGPCELPKINIRSYEESLESLGFEWLKSTGEKLDDLAWSVGGKVENVPYSYAGKTWSRFILANS
NGLYHSEKSNLISAGVALVATDGKTKKMGGYTRSGLDLDRIDPKLIVSTAAERSLAFLGAKPVKSGLYNIVLSNRITPQI
FGMFSSPFSADSVQKGLSRLEGKIGSQIASQNWNVFCDPHISDYPGSRLLDAEGVLTSKKAVIENGILQTYLYNLESAKK
EGVVPTGNAVRSYGGRVGASFNNYVVPKGDKTLEELLSGFPECIYVLKLEGGSGCSAVSGEISIGVQGIYYKNGIPVHPV
DSITMNLNFFDLLFRIEGISNEYNDSYSSIKVPDILIREANIAG

Specific function: May Facilitate The Secretion Of The Peptide Antibiotic Microcin B17 (Mccb17) By Completing Its Maturation. Suppresses The Inhibitory Activity Of The Carbon Storage Regulator (Csra). [C]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790682, Length=461, Percent_Identity=23.4273318872017, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789640, Length=425, Percent_Identity=23.7647058823529, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48485; Mature: 48485

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDSSVEYVLDICKKKRLDQYDLVGSESKEVGIELFRKRVSNTELSNSRGIGIRLIQSR
CCCCHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
KPGYSYSEKLSEEALSQMVEDALAQSKISDPLDIDLPGPCELPKINIRSYEESLESLGFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHH
WLKSTGEKLDDLAWSVGGKVENVPYSYAGKTWSRFILANSNGLYHSEKSNLISAGVALVA
HHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEEECCCCCEECCCCCHHHCCEEEEE
TDGKTKKMGGYTRSGLDLDRIDPKLIVSTAAERSLAFLGAKPVKSGLYNIVLSNRITPQI
ECCCCCCCCCCCCCCCCHHHCCCHHEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHH
FGMFSSPFSADSVQKGLSRLEGKIGSQIASQNWNVFCDPHISDYPGSRLLDAEGVLTSKK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEECCCCCHHHHH
AVIENGILQTYLYNLESAKKEGVVPTGNAVRSYGGRVGASFNNYVVPKGDKTLEELLSGF
HHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHCC
PECIYVLKLEGGSGCSAVSGEISIGVQGIYYKNGIPVHPVDSITMNLNFFDLLFRIEGIS
CCEEEEEEECCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCEEECCHHHHHHHHHHCCC
NEYNDSYSSIKVPDILIREANIAG
CCCCCCCCCEECCCEEEECCCCCC
>Mature Secondary Structure
MNLDSSVEYVLDICKKKRLDQYDLVGSESKEVGIELFRKRVSNTELSNSRGIGIRLIQSR
CCCCHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
KPGYSYSEKLSEEALSQMVEDALAQSKISDPLDIDLPGPCELPKINIRSYEESLESLGFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHH
WLKSTGEKLDDLAWSVGGKVENVPYSYAGKTWSRFILANSNGLYHSEKSNLISAGVALVA
HHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEEECCCCCEECCCCCHHHCCEEEEE
TDGKTKKMGGYTRSGLDLDRIDPKLIVSTAAERSLAFLGAKPVKSGLYNIVLSNRITPQI
ECCCCCCCCCCCCCCCCHHHCCCHHEEHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHH
FGMFSSPFSADSVQKGLSRLEGKIGSQIASQNWNVFCDPHISDYPGSRLLDAEGVLTSKK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEECCCCCHHHHH
AVIENGILQTYLYNLESAKKEGVVPTGNAVRSYGGRVGASFNNYVVPKGDKTLEELLSGF
HHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHCC
PECIYVLKLEGGSGCSAVSGEISIGVQGIYYKNGIPVHPVDSITMNLNFFDLLFRIEGIS
CCEEEEEEECCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCEEECCHHHHHHHHHHCCC
NEYNDSYSSIKVPDILIREANIAG
CCCCCCCCCEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]