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The map label for this gene is bioB

Identifier: 116328116

GI number: 116328116

Start: 1647077

End: 1648153

Strand: Direct

Name: bioB

Synonym: LBL_1414

Alternate gene names: 116328116

Gene position: 1647077-1648153 (Clockwise)

Preceding gene: 116328115

Following gene: 116328117

Centisome position: 45.57

GC content: 42.43

Gene sequence:

>1077_bases
CTGTCTGAAAAGATGTACGCAACACTTAAAACAGCCGAAAAAATATTCTCCGAAATTTCAAGCGTAATCACCAAACAAGA
GGGCTTGGAAATTTTAAACGGTTCCATTCCATTGACTACTTGTTTGGACAAGGCGTTTCAGGAAAGAAATCGATACTTCT
ACAATAAGGTCAGGATTCATATTTTAGATAACATCAAAAACGGTTATTGTCCGGAAGATTGCGGTTATTGCGCCCAAAGG
AAGAATGCAAATTCGGGAATCAAAGAATATCCAATGAAGTCCGAGGCCGAAATTTACGAAGACGCGGTTCAGGCTAAAAA
GAACGGAGCCTATCGTTTTTGTATGGTGACTTCCGGGACGGGCCCGAACCGACTTACCACTGAGAGACTCGCTTCTACAA
TTCGAAGGATTACGAACGAGCTTGGAATGAAAGTTTGTCTTTCTGCAGGTCTGTTAGATGAAGAAAAGGCGCAGGTTTTA
AAATCGGCCGGTTTGGATCGTTACAATCACAATCTCAATACCTCTGAAAATCATTATCCTGAAATCTGTGATACTCATAC
CTACGCACAAAGAACACAAACCTTGGATTCCGTTTCTAAGGCGGGGATCGGAATGTGTAGCGGGGTTATCGTAGGAATGG
GGGAATCGTTCCAAGATATAGTGGATATGGCTTTTGAACTTAAGTCTTTTCGAGTGATTTCAATTCCGGTGAATTTTTTC
ATTCCGGTCAAAGGGCACGCGATCAAAAATCCAGGCATTTTGACTCCCGAACTTTGTGTGAGAATACTTTGTCTTTTCCG
TTTAGTGAATCCGGATTCGGAAATTAGAATTGCAGCGGGAAGGGAAGGGCATCTTAGAAGCCTTTCTGCAATGGCTTTGT
TTGCCGCTAATTCTTTATTTTCTTCCGGTTATTTGAATGTAAAGGGCTCAGAGATAATCGAGACGGTCACGATGATTCGA
GATGCGGGTTTTGTCCCGGAGCTTGTGGATGGGGGAATTCTTCCGGAAGAATCTGGGACGGAAATGTTGTACTCGGAAAA
AAACTTCCCGGAACTTTACAAATTTAAGAAGTCTTGA

Upstream 100 bases:

>100_bases
AAAATTCTTCCTTGGAAAAAGTATTTTCTGCGATCCGGGAAACACTCTCGGAAATTTCCTATGGGAATTAGACTTTTCAA
GAAAGCTTCTTCTCTTCAAT

Downstream 100 bases:

>100_bases
AGTCTAAAAAATCCTTTAAAAAAGCTGTTTTTAGTATTTTTTAAGCTTTCCCGGCAACTTTTTTTTATTAAATTGAGTCT
GAGAACAATGTAGATTGTTA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSEKMYATLKTAEKIFSEISSVITKQEGLEILNGSIPLTTCLDKAFQERNRYFYNKVRIHILDNIKNGYCPEDCGYCAQR
KNANSGIKEYPMKSEAEIYEDAVQAKKNGAYRFCMVTSGTGPNRLTTERLASTIRRITNELGMKVCLSAGLLDEEKAQVL
KSAGLDRYNHNLNTSENHYPEICDTHTYAQRTQTLDSVSKAGIGMCSGVIVGMGESFQDIVDMAFELKSFRVISIPVNFF
IPVKGHAIKNPGILTPELCVRILCLFRLVNPDSEIRIAAGREGHLRSLSAMALFAANSLFSSGYLNVKGSEIIETVTMIR
DAGFVPELVDGGILPEESGTEMLYSEKNFPELYKFKKS

Sequences:

>Translated_358_residues
MSEKMYATLKTAEKIFSEISSVITKQEGLEILNGSIPLTTCLDKAFQERNRYFYNKVRIHILDNIKNGYCPEDCGYCAQR
KNANSGIKEYPMKSEAEIYEDAVQAKKNGAYRFCMVTSGTGPNRLTTERLASTIRRITNELGMKVCLSAGLLDEEKAQVL
KSAGLDRYNHNLNTSENHYPEICDTHTYAQRTQTLDSVSKAGIGMCSGVIVGMGESFQDIVDMAFELKSFRVISIPVNFF
IPVKGHAIKNPGILTPELCVRILCLFRLVNPDSEIRIAAGREGHLRSLSAMALFAANSLFSSGYLNVKGSEIIETVTMIR
DAGFVPELVDGGILPEESGTEMLYSEKNFPELYKFKKS
>Mature_357_residues
SEKMYATLKTAEKIFSEISSVITKQEGLEILNGSIPLTTCLDKAFQERNRYFYNKVRIHILDNIKNGYCPEDCGYCAQRK
NANSGIKEYPMKSEAEIYEDAVQAKKNGAYRFCMVTSGTGPNRLTTERLASTIRRITNELGMKVCLSAGLLDEEKAQVLK
SAGLDRYNHNLNTSENHYPEICDTHTYAQRTQTLDSVSKAGIGMCSGVIVGMGESFQDIVDMAFELKSFRVISIPVNFFI
PVKGHAIKNPGILTPELCVRILCLFRLVNPDSEIRIAAGREGHLRSLSAMALFAANSLFSSGYLNVKGSEIIETVTMIRD
AGFVPELVDGGILPEESGTEMLYSEKNFPELYKFKKS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=296, Percent_Identity=33.445945945946, Blast_Score=167, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6321725, Length=279, Percent_Identity=33.6917562724014, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_LEPBJ (Q04RS9)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801149.1
- ProteinModelPortal:   Q04RS9
- SMR:   Q04RS9
- STRING:   Q04RS9
- GeneID:   4410110
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1870
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q04RS9
- ProtClustDB:   CLSK574240
- BioCyc:   LBOR355277:LBJ_1870-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 39844; Mature: 39713

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKMYATLKTAEKIFSEISSVITKQEGLEILNGSIPLTTCLDKAFQERNRYFYNKVRIH
CCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHEEHHEEEE
ILDNIKNGYCPEDCGYCAQRKNANSGIKEYPMKSEAEIYEDAVQAKKNGAYRFCMVTSGT
EEECCCCCCCCHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCC
GPNRLTTERLASTIRRITNELGMKVCLSAGLLDEEKAQVLKSAGLDRYNHNLNTSENHYP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCC
EICDTHTYAQRTQTLDSVSKAGIGMCSGVIVGMGESFQDIVDMAFELKSFRVISIPVNFF
CHHCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEEEEEEE
IPVKGHAIKNPGILTPELCVRILCLFRLVNPDSEIRIAAGREGHLRSLSAMALFAANSLF
EECCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
SSGYLNVKGSEIIETVTMIRDAGFVPELVDGGILPEESGTEMLYSEKNFPELYKFKKS
HCCCEECCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEECCCCCHHHHHCCCC
>Mature Secondary Structure 
SEKMYATLKTAEKIFSEISSVITKQEGLEILNGSIPLTTCLDKAFQERNRYFYNKVRIH
CCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHEEHHEEEE
ILDNIKNGYCPEDCGYCAQRKNANSGIKEYPMKSEAEIYEDAVQAKKNGAYRFCMVTSGT
EEECCCCCCCCHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCC
GPNRLTTERLASTIRRITNELGMKVCLSAGLLDEEKAQVLKSAGLDRYNHNLNTSENHYP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCC
EICDTHTYAQRTQTLDSVSKAGIGMCSGVIVGMGESFQDIVDMAFELKSFRVISIPVNFF
CHHCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEEEEEEE
IPVKGHAIKNPGILTPELCVRILCLFRLVNPDSEIRIAAGREGHLRSLSAMALFAANSLF
EECCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
SSGYLNVKGSEIIETVTMIRDAGFVPELVDGGILPEESGTEMLYSEKNFPELYKFKKS
HCCCEECCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEECCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA