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The map label for this gene is gloB

Identifier: 116328093

GI number: 116328093

Start: 1622282

End: 1623091

Strand: Reverse

Name: gloB

Synonym: LBL_1390

Alternate gene names: 116328093

Gene position: 1623091-1622282 (Counterclockwise)

Preceding gene: 116328094

Following gene: 116328092

Centisome position: 44.91

GC content: 40.49

Gene sequence:

>810_bases
CTGGAAGTCTTTCGAATCTATACGAACAGTCCCCTCCGAAACTTCACCTATATTCTTAGAAACTCCGAAACCTCCGAAAC
TCTTTCCATCGATCCTTACGATTCCGAACAGATCGAAAAATTTCTGGATTCAAAAGGTTGGACATTAGACTTTCTTCTGA
ATACGCACGAACACGAAGACCATACGTCCGGTAACACAGGGCTTGTTCAAAGATACGGCTGCACGGTATATTCTCATCCC
GAAGGTATCGGCAAAATTCCACACGCGACTCATCCTCTAAAGAAAGGAGATTTCCTTCTTCGTTCTTCTAAAGAATACTT
GGAAATTCTAGATACTCCCGGTCATACATTTTGTCATGTGTGTTTACTTCTCGTTGAAAATCAAAAACCAAAGGCGATTT
TTACAGGGGATACAATCTTTAACGCGGGAGTCGGAAACTGTCATCACGGCGGCGACCCGGAAGTTCTTGCAAAAACGATT
TTGGAACAATTCTATCCTTTGGAAGAAGAAATTCTTCTTTATCCCGGCCATGATTATCTCGAAACGAATTTAAAATTTAC
ACTTTCCCTTGACCCTTCCAATCAAGATGCAATTCGTACCTTGGAAGAATGTTCCCGCTTATCCAAAAACGTGGAATTTC
TCACTACCGACCTTAGGAAAGAAAGAAAAATCAACACGTTTTTCCAGTGCGATAAACCTTCCCTTGAACTTCGAAAAAAC
GTCTCAAAAAAGATCCCTTTTAAACAGCTTCTCGATAATGATCCAACTTCCTTTTTTATTTCTCTCCGTTCTTTACGAGA
TCAATGGTAA

Upstream 100 bases:

>100_bases
CGGTTCTCTCAAGCTCAAAAACATCAGCAGAGACGTGATGACAATTTTGAAAATCGCGAATCTGGACAAACACTTAGATA
TCATTCACTAAGGTAGTTTT

Downstream 100 bases:

>100_bases
ATAGTTTGCTCTCTTGACCACAGGATTTAGAATCTGTAACCAAGGCGATTCTTTTTATGAAAGAATATGATATCATCGTA
ATTGGTACGGGAGGAGGAAC

Product: Zn-dependent hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MEVFRIYTNSPLRNFTYILRNSETSETLSIDPYDSEQIEKFLDSKGWTLDFLLNTHEHEDHTSGNTGLVQRYGCTVYSHP
EGIGKIPHATHPLKKGDFLLRSSKEYLEILDTPGHTFCHVCLLLVENQKPKAIFTGDTIFNAGVGNCHHGGDPEVLAKTI
LEQFYPLEEEILLYPGHDYLETNLKFTLSLDPSNQDAIRTLEECSRLSKNVEFLTTDLRKERKINTFFQCDKPSLELRKN
VSKKIPFKQLLDNDPTSFFISLRSLRDQW

Sequences:

>Translated_269_residues
MEVFRIYTNSPLRNFTYILRNSETSETLSIDPYDSEQIEKFLDSKGWTLDFLLNTHEHEDHTSGNTGLVQRYGCTVYSHP
EGIGKIPHATHPLKKGDFLLRSSKEYLEILDTPGHTFCHVCLLLVENQKPKAIFTGDTIFNAGVGNCHHGGDPEVLAKTI
LEQFYPLEEEILLYPGHDYLETNLKFTLSLDPSNQDAIRTLEECSRLSKNVEFLTTDLRKERKINTFFQCDKPSLELRKN
VSKKIPFKQLLDNDPTSFFISLRSLRDQW
>Mature_269_residues
MEVFRIYTNSPLRNFTYILRNSETSETLSIDPYDSEQIEKFLDSKGWTLDFLLNTHEHEDHTSGNTGLVQRYGCTVYSHP
EGIGKIPHATHPLKKGDFLLRSSKEYLEILDTPGHTFCHVCLLLVENQKPKAIFTGDTIFNAGVGNCHHGGDPEVLAKTI
LEQFYPLEEEILLYPGHDYLETNLKFTLSLDPSNQDAIRTLEECSRLSKNVEFLTTDLRKERKINTFFQCDKPSLELRKN
VSKKIPFKQLLDNDPTSFFISLRSLRDQW

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI21703352, Length=221, Percent_Identity=31.6742081447964, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI116642887, Length=221, Percent_Identity=31.6742081447964, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI94538322, Length=261, Percent_Identity=29.1187739463602, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI94538320, Length=261, Percent_Identity=28.735632183908, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI14150041, Length=238, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI46361987, Length=190, Percent_Identity=30, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1786406, Length=259, Percent_Identity=28.1853281853282, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787158, Length=199, Percent_Identity=28.1407035175879, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=247, Percent_Identity=30.3643724696356, Blast_Score=104, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320478, Length=242, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24667703, Length=228, Percent_Identity=25.4385964912281, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356335, Length=228, Percent_Identity=25.4385964912281, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24667711, Length=228, Percent_Identity=25.4385964912281, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI221330176, Length=180, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_LEPBJ (Q04RQ6)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801172.1
- ProteinModelPortal:   Q04RQ6
- SMR:   Q04RQ6
- STRING:   Q04RQ6
- GeneID:   4412170
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1894
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   DHWLALE
- PhylomeDB:   Q04RQ6
- ProtClustDB:   CLSK574219
- BioCyc:   LBOR355277:LBJ_1894-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 31016; Mature: 31016

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVFRIYTNSPLRNFTYILRNSETSETLSIDPYDSEQIEKFLDSKGWTLDFLLNTHEHED
CCEEEEECCCCHHHEEEEEECCCCCCEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCCC
HTSGNTGLVQRYGCTVYSHPEGIGKIPHATHPLKKGDFLLRSSKEYLEILDTPGHTFCHV
CCCCCCCEEECCCCEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCHHHHHH
CLLLVENQKPKAIFTGDTIFNAGVGNCHHGGDPEVLAKTILEQFYPLEEEILLYPGHDYL
HHHHHCCCCCCEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEE
ETNLKFTLSLDPSNQDAIRTLEECSRLSKNVEFLTTDLRKERKINTFFQCDKPSLELRKN
ECCEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHH
VSKKIPFKQLLDNDPTSFFISLRSLRDQW
HHHCCCHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MEVFRIYTNSPLRNFTYILRNSETSETLSIDPYDSEQIEKFLDSKGWTLDFLLNTHEHED
CCEEEEECCCCHHHEEEEEECCCCCCEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCCC
HTSGNTGLVQRYGCTVYSHPEGIGKIPHATHPLKKGDFLLRSSKEYLEILDTPGHTFCHV
CCCCCCCEEECCCCEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCHHHHHH
CLLLVENQKPKAIFTGDTIFNAGVGNCHHGGDPEVLAKTILEQFYPLEEEILLYPGHDYL
HHHHHCCCCCCEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEE
ETNLKFTLSLDPSNQDAIRTLEECSRLSKNVEFLTTDLRKERKINTFFQCDKPSLELRKN
ECCEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHH
VSKKIPFKQLLDNDPTSFFISLRSLRDQW
HHHCCCHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA