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The map label for this gene is lpdA-3 [H]

Identifier: 116328092

GI number: 116328092

Start: 1620843

End: 1622225

Strand: Reverse

Name: lpdA-3 [H]

Synonym: LBL_1389

Alternate gene names: 116328092

Gene position: 1622225-1620843 (Counterclockwise)

Preceding gene: 116328093

Following gene: 116328091

Centisome position: 44.88

GC content: 41.36

Gene sequence:

>1383_bases
ATGAAAGAATATGATATCATCGTAATTGGTACGGGAGGAGGAACCAAACTGATCACTCCTCCTTCTAAAATCGGTTATAA
AGTCGCGGTAATCGAAAAAGAATCTCCCGGAGGAACCTGCCTCAATCGGGGATGTATTCCTTCCAAGATGTTGATTTATC
CGGCTGAAATTCTTTCTCTTGCTAAGAATTCGGAGAAATTTCAAATCTCATTTCCCGGCAAACCTAAAGTCGATTTTAAA
TCTCTTGTAGAAAGAGTTTCAAAAACGGTGGACGAGGAATCCGCATCAATTCGTCCGGCATACGATAAAAATCCGAATAT
CAACTACATTCAAGGAACCGCAAGTTTTGTTTCAGATAAGGTGATCGTCGTAAACGGAGAACAACTGACTGCAAAAAAAA
TTTTTATCGCGTCAGGGGCAAGACCCTCAATCCCGAATATCCCGGGTCTTGTCGGAACTCCTTATATGACGAGTAGAGAA
GCCCTTCGCAGGACCGATCTCCCAAAATCATTGATCGTAATCGGAGGCGGTTTTATCGCTTTGGAATTAGGTTTTGCCTA
CTCTGCATTTGGAAGCGAGGTTACTTTTTTAGTTCGAAGTAGGATGCTCAAAAACGAAGACGGGGATATCGTAAACGAAT
TTGAACGGGTTTTTACCAAAGAACACAATGTACTACTCCGCACAGATATTCATAAATTAGAATATAAAGAAAAAATGTTT
CATGTAGAAGTCGAAACGGCTGGAAAAAAATTACAACTCCGATCGGAAGCGCTCTTGGTCGCAACCGGAATCCGGCCCAA
TACCGATCTTTTAAATTTGTCGAATACGAAAATCCAAACCGATACAAACGGGTACATTATCGTAAACGAATATTTAGAAA
CCACTTCTCCCGAAGTTTACGCATTAGGCGATATCACCGGAAAATATTTTTATAGACATTCCGTAAATTTCGAAGGGGAA
TTTTTATTCCGCACCTTATACTTGGAAAAGAAAAGAACTCCGATCGATTATCCTCCGGTTCCACACGCCGTTTTTACGCA
TCCTCAGGTGGCCAGAGTAGGAAAAACGGAAGAACAACTGGTTCAAGAAGGAATCGATTATATCGCCGCTAAAAATCCGT
ACAGTGCCAGCGCTACCGGAATGGCGCGGCTTTCCGATTCCGGATTCGTAAAAATTCTAGTGGATAAAAAATCGAGAAAG
GTTTTAGGAGCACACGCAATCGGAGACGAAGCTTCCAATGTGATTCATCTTTTTATCCTCTTGATGACTATCGGGGGAAA
TTTGGACGATCTTTTAAGAATGATATATATACATCCTGCGTTACCCGAAATTGCACGTAATGCGGCCAGAAAAGCCGGTG
AACTTTTGCAAATAAAAGAATAA

Upstream 100 bases:

>100_bases
CTTCCTTTTTTATTTCTCTCCGTTCTTTACGAGATCAATGGTAAATAGTTTGCTCTCTTGACCACAGGATTTAGAATCTG
TAACCAAGGCGATTCTTTTT

Downstream 100 bases:

>100_bases
TGGGAAAATTTAGAATTTATGAAATTTGCTCTGCAAATTGAGAATTTAGTCAAAACGTACGCAGGTGGAGTCACCGCCCT
TAAAGGAATCAACCTTAACG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKEYDIIVIGTGGGTKLITPPSKIGYKVAVIEKESPGGTCLNRGCIPSKMLIYPAEILSLAKNSEKFQISFPGKPKVDFK
SLVERVSKTVDEESASIRPAYDKNPNINYIQGTASFVSDKVIVVNGEQLTAKKIFIASGARPSIPNIPGLVGTPYMTSRE
ALRRTDLPKSLIVIGGGFIALELGFAYSAFGSEVTFLVRSRMLKNEDGDIVNEFERVFTKEHNVLLRTDIHKLEYKEKMF
HVEVETAGKKLQLRSEALLVATGIRPNTDLLNLSNTKIQTDTNGYIIVNEYLETTSPEVYALGDITGKYFYRHSVNFEGE
FLFRTLYLEKKRTPIDYPPVPHAVFTHPQVARVGKTEEQLVQEGIDYIAAKNPYSASATGMARLSDSGFVKILVDKKSRK
VLGAHAIGDEASNVIHLFILLMTIGGNLDDLLRMIYIHPALPEIARNAARKAGELLQIKE

Sequences:

>Translated_460_residues
MKEYDIIVIGTGGGTKLITPPSKIGYKVAVIEKESPGGTCLNRGCIPSKMLIYPAEILSLAKNSEKFQISFPGKPKVDFK
SLVERVSKTVDEESASIRPAYDKNPNINYIQGTASFVSDKVIVVNGEQLTAKKIFIASGARPSIPNIPGLVGTPYMTSRE
ALRRTDLPKSLIVIGGGFIALELGFAYSAFGSEVTFLVRSRMLKNEDGDIVNEFERVFTKEHNVLLRTDIHKLEYKEKMF
HVEVETAGKKLQLRSEALLVATGIRPNTDLLNLSNTKIQTDTNGYIIVNEYLETTSPEVYALGDITGKYFYRHSVNFEGE
FLFRTLYLEKKRTPIDYPPVPHAVFTHPQVARVGKTEEQLVQEGIDYIAAKNPYSASATGMARLSDSGFVKILVDKKSRK
VLGAHAIGDEASNVIHLFILLMTIGGNLDDLLRMIYIHPALPEIARNAARKAGELLQIKE
>Mature_460_residues
MKEYDIIVIGTGGGTKLITPPSKIGYKVAVIEKESPGGTCLNRGCIPSKMLIYPAEILSLAKNSEKFQISFPGKPKVDFK
SLVERVSKTVDEESASIRPAYDKNPNINYIQGTASFVSDKVIVVNGEQLTAKKIFIASGARPSIPNIPGLVGTPYMTSRE
ALRRTDLPKSLIVIGGGFIALELGFAYSAFGSEVTFLVRSRMLKNEDGDIVNEFERVFTKEHNVLLRTDIHKLEYKEKMF
HVEVETAGKKLQLRSEALLVATGIRPNTDLLNLSNTKIQTDTNGYIIVNEYLETTSPEVYALGDITGKYFYRHSVNFEGE
FLFRTLYLEKKRTPIDYPPVPHAVFTHPQVARVGKTEEQLVQEGIDYIAAKNPYSASATGMARLSDSGFVKILVDKKSRK
VLGAHAIGDEASNVIHLFILLMTIGGNLDDLLRMIYIHPALPEIARNAARKAGELLQIKE

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=466, Percent_Identity=30.68669527897, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI50301238, Length=441, Percent_Identity=29.2517006802721, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI22035672, Length=450, Percent_Identity=26.8888888888889, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI148277065, Length=461, Percent_Identity=25.1626898047722, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI33519430, Length=461, Percent_Identity=25.1626898047722, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI33519428, Length=461, Percent_Identity=25.1626898047722, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI33519426, Length=461, Percent_Identity=25.1626898047722, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI148277071, Length=461, Percent_Identity=25.1626898047722, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI291045266, Length=433, Percent_Identity=26.0969976905312, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI291045268, Length=425, Percent_Identity=24, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI1786307, Length=449, Percent_Identity=30.0668151447661, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI87081717, Length=454, Percent_Identity=30.1762114537445, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789915, Length=426, Percent_Identity=29.3427230046948, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082354, Length=469, Percent_Identity=27.2921108742004, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789065, Length=242, Percent_Identity=22.7272727272727, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=33.7662337662338, Blast_Score=231, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=29.746835443038, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71983429, Length=434, Percent_Identity=26.7281105990783, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71983419, Length=434, Percent_Identity=26.7281105990783, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71982272, Length=480, Percent_Identity=26.0416666666667, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=469, Percent_Identity=29.637526652452, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6325166, Length=445, Percent_Identity=26.5168539325843, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=23.6897274633124, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21358499, Length=462, Percent_Identity=34.4155844155844, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24640549, Length=472, Percent_Identity=29.0254237288136, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24640553, Length=472, Percent_Identity=29.0254237288136, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24640551, Length=473, Percent_Identity=29.3868921775898, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17737741, Length=473, Percent_Identity=24.7357293868922, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 50926; Mature: 50926

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEYDIIVIGTGGGTKLITPPSKIGYKVAVIEKESPGGTCLNRGCIPSKMLIYPAEILSL
CCCEEEEEEECCCCCEEECCCHHHCEEEEEEEECCCCCCEECCCCCCCCCEEEHHHHHHH
AKNSEKFQISFPGKPKVDFKSLVERVSKTVDEESASIRPAYDKNPNINYIQGTASFVSDK
HCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHCCC
VIVVNGEQLTAKKIFIASGARPSIPNIPGLVGTPYMTSREALRRTDLPKSLIVIGGGFIA
EEEEECCEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCEEE
LELGFAYSAFGSEVTFLVRSRMLKNEDGDIVNEFERVFTKEHNVLLRTDIHKLEYKEKMF
EEECCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHHEE
HVEVETAGKKLQLRSEALLVATGIRPNTDLLNLSNTKIQTDTNGYIIVNEYLETTSPEVY
EEEEECCCCEEEECCCEEEEEECCCCCCCEEECCCCEEEECCCCEEEEECHHCCCCCCEE
ALGDITGKYFYRHSVNFEGEFLFRTLYLEKKRTPIDYPPVPHAVFTHPQVARVGKTEEQL
EEECCCCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCHHCCCCHHHHCCCCHHHH
VQEGIDYIAAKNPYSASATGMARLSDSGFVKILVDKKSRKVLGAHAIGDEASNVIHLFIL
HHHHHHHEECCCCCCCCCCCEEEECCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHH
LMTIGGNLDDLLRMIYIHPALPEIARNAARKAGELLQIKE
HHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MKEYDIIVIGTGGGTKLITPPSKIGYKVAVIEKESPGGTCLNRGCIPSKMLIYPAEILSL
CCCEEEEEEECCCCCEEECCCHHHCEEEEEEEECCCCCCEECCCCCCCCCEEEHHHHHHH
AKNSEKFQISFPGKPKVDFKSLVERVSKTVDEESASIRPAYDKNPNINYIQGTASFVSDK
HCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHCCC
VIVVNGEQLTAKKIFIASGARPSIPNIPGLVGTPYMTSREALRRTDLPKSLIVIGGGFIA
EEEEECCEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCEEE
LELGFAYSAFGSEVTFLVRSRMLKNEDGDIVNEFERVFTKEHNVLLRTDIHKLEYKEKMF
EEECCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHHEE
HVEVETAGKKLQLRSEALLVATGIRPNTDLLNLSNTKIQTDTNGYIIVNEYLETTSPEVY
EEEEECCCCEEEECCCEEEEEECCCCCCCEEECCCCEEEECCCCEEEEECHHCCCCCCEE
ALGDITGKYFYRHSVNFEGEFLFRTLYLEKKRTPIDYPPVPHAVFTHPQVARVGKTEEQL
EEECCCCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCHHCCCCHHHHCCCCHHHH
VQEGIDYIAAKNPYSASATGMARLSDSGFVKILVDKKSRKVLGAHAIGDEASNVIHLFIL
HHHHHHHEECCCCCCCCCCCEEEECCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHH
LMTIGGNLDDLLRMIYIHPALPEIARNAARKAGELLQIKE
HHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3037534 [H]