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The map label for this gene is gshA [H]

Identifier: 116328084

GI number: 116328084

Start: 1615391

End: 1616953

Strand: Direct

Name: gshA [H]

Synonym: LBL_1381

Alternate gene names: 116328084

Gene position: 1615391-1616953 (Clockwise)

Preceding gene: 116328083

Following gene: 116328085

Centisome position: 44.69

GC content: 40.95

Gene sequence:

>1563_bases
TTGAAAACTAAGGAACTCACTCAATCGAAGATAGAAGAAGTATCCCTCGAAATTCTTTTGAGACATGCCGTAAAGGCGAA
ACACGGCTTGGAAAAGGAAAGTATGAGGGTGGGACCCGACGGAATGCTCGCGAAAACTCCGCATCCGGCGCACTTGGGTT
CGAGTTTAACCAACCACTATATCAAAACCGATTTTGCGGAGCCTCAGCTCGAATACGCGACTCATCCTCGTCCTAAGATT
GAAGCGAATATTCGTGAATTACAAGATTTGCATATATATACGTTTCGTCGATTGGACAACGAACTCATCTGGCCTTTCAG
TATGCCTCCGATTCTACCGAAGGATGAAAACGAAATTCCTCTTGGGCAATACGGGACTTCCCATTCCGGGAGATGGAAAA
CGATTTATCGTCGCGGATTGGGGCTTCGTTACGGAAGGAGAATGCAGACTATCTCCGGAGTTCACTATAACTTTTCTTTC
TCGAAAATTTTCCTTCGTCAATTTTTAGGAAAAGAAATTTCGAACTTTACGAAAGAGGAAATCTCTTCTCTTTATCTGCA
TGTGATTCGAAATTTTATGAGAAGGGTTCATTATTTAACGTATCTCACCGGGTCTTCCGCCGTTTTCGATTCCACTTTTT
TGCCGAATCCGGGAAACCTCAAATTTAAAAAACATAAGAATTTTACGTTATACTCTCCTTATGCCACTTCGCTCAGGATG
AGCGAGATCGGGTATACGAGTAAGGTTCAGGATACATTAGGAATTCATTATAATTCCTTGGAAGAATATGCGGAACGGAT
GTGTTACGCGGTTCATACCCCGTATCCTAGTTATGTTTCTTTTTCTCAAAATGCGGACGCTCAACTCAATCCGAATTATC
TTCAGATCGAAAACGAATTTTATTCTCCGATTCGTCCGAAACAGGTTCCTAAGGGGGATGAAAGACCGTTAGATGCTCTT
CTTCGAAGGGGAATCGAATACATAGAGATTCGTTCTTTGGACATCGATCCTTATTCTCCTGTTGGAGTTTGTAGGCTTAA
TCTCGCCTTTACCCAGTTGATTCTTTTGGACTCTTTGCTTAGCCCTTCTCCATTGATTTCCGAAGAAGAGAATATGATCT
TAAAAGAAAATTTAAACTCCGTGATTTGGGAAGGAAGAAATCCGGAATTGGAAATCAATTCGAACGGAACCAAGAAAAAC
TTTCAAGCAGCCGGAGCGGAATACTCGGAGTCCCTGAGACACTACGCAAAAATTTTGGATCTTCATACCAAAAGACGTAT
GTATCAGGAAGCAATCGACTTTCAGATTAAAAAATGGAAAAATCCGGATAAAACCCCTTCCGGAAAATTACTTTCCGAAA
TTCTAAAACGTAATATTGAATTCAGAGATAGGGGAATGGAACTTGCTCGTGAAAATCGACGCGCTCTTTCTTATTTGGAA
TATTCTCCCGGAACTTTGATGAAGATAGAAAAAGAAGCGGTTCGTTCCTTTCAAGAAAAAGAACAATTGGAAAAAGAAGA
AATCCAAACTCAATATCCCACCGTTAAACTATGCAATCACTGA

Upstream 100 bases:

>100_bases
GAATTGCTCCGAACTTTGCAAAACTGTTCTCGGTCAAAAGAGAGAACGGAATGCTCTATGGAGCTTCCGATCCACGTGGG
GAAGGAGCCACGGGCGGACT

Downstream 100 bases:

>100_bases
AATTAAAGCCTGGTGAGTTTTTTTCCCCGGAAATATTTACTTTGAAAGGATTCGAGGATCTGGAAATTTCCACGCAGATT
GTGATTCGAGACGCACTCAA

Product: gamma-glutamylcysteine synthetase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKTKELTQSKIEEVSLEILLRHAVKAKHGLEKESMRVGPDGMLAKTPHPAHLGSSLTNHYIKTDFAEPQLEYATHPRPKI
EANIRELQDLHIYTFRRLDNELIWPFSMPPILPKDENEIPLGQYGTSHSGRWKTIYRRGLGLRYGRRMQTISGVHYNFSF
SKIFLRQFLGKEISNFTKEEISSLYLHVIRNFMRRVHYLTYLTGSSAVFDSTFLPNPGNLKFKKHKNFTLYSPYATSLRM
SEIGYTSKVQDTLGIHYNSLEEYAERMCYAVHTPYPSYVSFSQNADAQLNPNYLQIENEFYSPIRPKQVPKGDERPLDAL
LRRGIEYIEIRSLDIDPYSPVGVCRLNLAFTQLILLDSLLSPSPLISEEENMILKENLNSVIWEGRNPELEINSNGTKKN
FQAAGAEYSESLRHYAKILDLHTKRRMYQEAIDFQIKKWKNPDKTPSGKLLSEILKRNIEFRDRGMELARENRRALSYLE
YSPGTLMKIEKEAVRSFQEKEQLEKEEIQTQYPTVKLCNH

Sequences:

>Translated_520_residues
MKTKELTQSKIEEVSLEILLRHAVKAKHGLEKESMRVGPDGMLAKTPHPAHLGSSLTNHYIKTDFAEPQLEYATHPRPKI
EANIRELQDLHIYTFRRLDNELIWPFSMPPILPKDENEIPLGQYGTSHSGRWKTIYRRGLGLRYGRRMQTISGVHYNFSF
SKIFLRQFLGKEISNFTKEEISSLYLHVIRNFMRRVHYLTYLTGSSAVFDSTFLPNPGNLKFKKHKNFTLYSPYATSLRM
SEIGYTSKVQDTLGIHYNSLEEYAERMCYAVHTPYPSYVSFSQNADAQLNPNYLQIENEFYSPIRPKQVPKGDERPLDAL
LRRGIEYIEIRSLDIDPYSPVGVCRLNLAFTQLILLDSLLSPSPLISEEENMILKENLNSVIWEGRNPELEINSNGTKKN
FQAAGAEYSESLRHYAKILDLHTKRRMYQEAIDFQIKKWKNPDKTPSGKLLSEILKRNIEFRDRGMELARENRRALSYLE
YSPGTLMKIEKEAVRSFQEKEQLEKEEIQTQYPTVKLCNH
>Mature_520_residues
MKTKELTQSKIEEVSLEILLRHAVKAKHGLEKESMRVGPDGMLAKTPHPAHLGSSLTNHYIKTDFAEPQLEYATHPRPKI
EANIRELQDLHIYTFRRLDNELIWPFSMPPILPKDENEIPLGQYGTSHSGRWKTIYRRGLGLRYGRRMQTISGVHYNFSF
SKIFLRQFLGKEISNFTKEEISSLYLHVIRNFMRRVHYLTYLTGSSAVFDSTFLPNPGNLKFKKHKNFTLYSPYATSLRM
SEIGYTSKVQDTLGIHYNSLEEYAERMCYAVHTPYPSYVSFSQNADAQLNPNYLQIENEFYSPIRPKQVPKGDERPLDAL
LRRGIEYIEIRSLDIDPYSPVGVCRLNLAFTQLILLDSLLSPSPLISEEENMILKENLNSVIWEGRNPELEINSNGTKKN
FQAAGAEYSESLRHYAKILDLHTKRRMYQEAIDFQIKKWKNPDKTPSGKLLSEILKRNIEFRDRGMELARENRRALSYLE
YSPGTLMKIEKEAVRSFQEKEQLEKEEIQTQYPTVKLCNH

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=501, Percent_Identity=40.9181636726547, Blast_Score=341, Evalue=7e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 60524; Mature: 60524

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKELTQSKIEEVSLEILLRHAVKAKHGLEKESMRVGPDGMLAKTPHPAHLGSSLTNHY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEECCCCCEECCCCCHHHHHHHHHHH
IKTDFAEPQLEYATHPRPKIEANIRELQDLHIYTFRRLDNELIWPFSMPPILPKDENEIP
HHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCC
LGQYGTSHSGRWKTIYRRGLGLRYGRRMQTISGVHYNFSFSKIFLRQFLGKEISNFTKEE
CCCCCCCCCCCHHHHHHHCCCCHHCCHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHH
ISSLYLHVIRNFMRRVHYLTYLTGSSAVFDSTFLPNPGNLKFKKHKNFTLYSPYATSLRM
HHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEECCCEEEECCHHHHEEH
SEIGYTSKVQDTLGIHYNSLEEYAERMCYAVHTPYPSYVSFSQNADAQLNPNYLQIENEF
HHCCCCHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCCEECCCCCCCCCCCCEEEEEHHH
YSPIRPKQVPKGDERPLDALLRRGIEYIEIRSLDIDPYSPVGVCRLNLAFTQLILLDSLL
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCHHEEHHHHHHHHHHHHHHC
SPSPLISEEENMILKENLNSVIWEGRNPELEINSNGTKKNFQAAGAEYSESLRHYAKILD
CCCCCCCCCCCCHHHHCCHHHEECCCCCCEEECCCCCCCHHHHCCHHHHHHHHHHHHHHH
LHTKRRMYQEAIDFQIKKWKNPDKTPSGKLLSEILKRNIEFRDRGMELARENRRALSYLE
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
YSPGTLMKIEKEAVRSFQEKEQLEKEEIQTQYPTVKLCNH
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
>Mature Secondary Structure
MKTKELTQSKIEEVSLEILLRHAVKAKHGLEKESMRVGPDGMLAKTPHPAHLGSSLTNHY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEECCCCCEECCCCCHHHHHHHHHHH
IKTDFAEPQLEYATHPRPKIEANIRELQDLHIYTFRRLDNELIWPFSMPPILPKDENEIP
HHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCC
LGQYGTSHSGRWKTIYRRGLGLRYGRRMQTISGVHYNFSFSKIFLRQFLGKEISNFTKEE
CCCCCCCCCCCHHHHHHHCCCCHHCCHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHH
ISSLYLHVIRNFMRRVHYLTYLTGSSAVFDSTFLPNPGNLKFKKHKNFTLYSPYATSLRM
HHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEECCCEEEECCHHHHEEH
SEIGYTSKVQDTLGIHYNSLEEYAERMCYAVHTPYPSYVSFSQNADAQLNPNYLQIENEF
HHCCCCHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCCEECCCCCCCCCCCCEEEEEHHH
YSPIRPKQVPKGDERPLDALLRRGIEYIEIRSLDIDPYSPVGVCRLNLAFTQLILLDSLL
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCHHEEHHHHHHHHHHHHHHC
SPSPLISEEENMILKENLNSVIWEGRNPELEINSNGTKKNFQAAGAEYSESLRHYAKILD
CCCCCCCCCCCCHHHHCCHHHEECCCCCCEEECCCCCCCHHHHCCHHHHHHHHHHHHHHH
LHTKRRMYQEAIDFQIKKWKNPDKTPSGKLLSEILKRNIEFRDRGMELARENRRALSYLE
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
YSPGTLMKIEKEAVRSFQEKEQLEKEEIQTQYPTVKLCNH
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA