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The map label for this gene is pdhC [H]

Identifier: 116328021

GI number: 116328021

Start: 1545027

End: 1546442

Strand: Reverse

Name: pdhC [H]

Synonym: LBL_1316

Alternate gene names: 116328021

Gene position: 1546442-1545027 (Counterclockwise)

Preceding gene: 116328022

Following gene: 116328020

Centisome position: 42.79

GC content: 49.44

Gene sequence:

>1416_bases
ATGGCTAAAATTGCAGAGATGACTCAACTCAGTCCCACGATGTCGGAAGGAAAGATCGTCCGATGGCTCAAACAAAAAGG
AGATTCCGTTTCTCCCGGAGAAATCATCGCGGAGGTGGAAACGGACAAAGCCGTCATGGAAATGGAAGCCTTTGAAACCG
GTGTTCTTTTGGAAATTTTGGCTCCGGAAGGAAGCCTTCTTCCGGTCGGGGCGCCCGTCGCAATCATCGGGAAACCGGGA
GAAGATGTTTCTGCATTAGTCGAAATCGCAAAGAAATCCATTCCCGCCAAAAAGGAAGGTTCCGCCGCGCCTAGCCAAAC
TTCAACCTCAACACAAAGCACGTCATCCTCAACAGCTCCGACTTCTTCCGCACAAAGTGGAACTTCTGCCTCAACGTCCG
GCGCAGGCGCAGGTAAAAATCTCGAAACAAAAACAAGCGTTTCTCTTTCGCAGAATGCCGGAAACAGCGGCATAACCGCA
AACGAAGAACGAACTTACGGATTCAAAACGTCGCCCTCATTCGGCCCGGGAGAATCGGAACATTCTCCTGTTCGATCCGC
ACGCGGCGGACGTCCGATCAAAGCCTCCCCTTTGGCAAAAAATCTCGCCCTTCAAAAAGGAATCAATCTCACAGAAGTGA
TCGGCTCTGGTCCCGGAGGAAGGATCATCAAACGAGACATTCTCTCCTATCAGTCAGGTGGCGGAAATACTTTTGTTAAG
CGACAAGACCGTAAGTTGGAAATCACGGGAATGAGAAAGACAATCGCTTCCCGTCTTGCACATTCCACTTCGACCATCCC
TCATTTTTATCTTACGACGGAATTGAATGCGGGTCCGATCGACGATCTCAGAAACTCCATCAATAGGGATCTCGGACTCA
GCGGCCAGGGAAAAGTCAGCGTAAACGATCTCATTCTCAAAGCGTGTTCTTATACTTTGTTGCAAGTCCCGGAAGTGAAT
TCTTCCTGGAGAGAAGATCATATCTTAGAGCACGGAAGAGTCGATATCGGAGTCGCAGTTTCCATCGAAGGAGGTCTCAT
TACTCCTTATATTCGAAACGCAGAAGAGAAATCCGTCCTCGAAATCAGTCGAGAGATAAAAGAACTTGCGTCCCGGGCAA
GAGACAGAAAACTCAAACCGGGGGAATACACGGACGGCACCTTTACCGTCTCCAACCTCGGAATGTTTGGAGTCTCTTCC
TTCACCGCGGTCATCAACGAACCCGAAGCGGCAATTCTTGCGGTAGGCGCTCTCGTTGAAAAACCGGTGCTCAAGGCTGG
AAACATCGTTCCGGGAAAAATCTTGAACGTTACGCTTTCTTGTGATCACAGAGTCATAGATGGAGCGACGGGATCTAGAT
TTCTTTCCTTATTCCGAGAGTTTATGGAGCATCCCCTTCGCCTACTTACAGGCTAA

Upstream 100 bases:

>100_bases
CGTATGCCGCAAATTTGGAAAAGGCCTCTCTTCCGAGTGAAGAAAAAATCATCGCGAAAGTCCGAGAGATGCTCGAATAA
CGGAGCAAGGAAGATTCAAA

Downstream 100 bases:

>100_bases
GGATTTTATTTTAGTGGAAAAAGAATCGGCTCGAAAAGAAAAAATCAGAAAATCAGCCCTGCTCCTTCTCTCTTTAAATA
AAGAAGACGCCGCCAAAGTT

Product: bifunctional dihydrolipoyllysine-residue acetyltransferase/dihydrolipoyllysine-residue succinyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAKIAEMTQLSPTMSEGKIVRWLKQKGDSVSPGEIIAEVETDKAVMEMEAFETGVLLEILAPEGSLLPVGAPVAIIGKPG
EDVSALVEIAKKSIPAKKEGSAAPSQTSTSTQSTSSSTAPTSSAQSGTSASTSGAGAGKNLETKTSVSLSQNAGNSGITA
NEERTYGFKTSPSFGPGESEHSPVRSARGGRPIKASPLAKNLALQKGINLTEVIGSGPGGRIIKRDILSYQSGGGNTFVK
RQDRKLEITGMRKTIASRLAHSTSTIPHFYLTTELNAGPIDDLRNSINRDLGLSGQGKVSVNDLILKACSYTLLQVPEVN
SSWREDHILEHGRVDIGVAVSIEGGLITPYIRNAEEKSVLEISREIKELASRARDRKLKPGEYTDGTFTVSNLGMFGVSS
FTAVINEPEAAILAVGALVEKPVLKAGNIVPGKILNVTLSCDHRVIDGATGSRFLSLFREFMEHPLRLLTG

Sequences:

>Translated_471_residues
MAKIAEMTQLSPTMSEGKIVRWLKQKGDSVSPGEIIAEVETDKAVMEMEAFETGVLLEILAPEGSLLPVGAPVAIIGKPG
EDVSALVEIAKKSIPAKKEGSAAPSQTSTSTQSTSSSTAPTSSAQSGTSASTSGAGAGKNLETKTSVSLSQNAGNSGITA
NEERTYGFKTSPSFGPGESEHSPVRSARGGRPIKASPLAKNLALQKGINLTEVIGSGPGGRIIKRDILSYQSGGGNTFVK
RQDRKLEITGMRKTIASRLAHSTSTIPHFYLTTELNAGPIDDLRNSINRDLGLSGQGKVSVNDLILKACSYTLLQVPEVN
SSWREDHILEHGRVDIGVAVSIEGGLITPYIRNAEEKSVLEISREIKELASRARDRKLKPGEYTDGTFTVSNLGMFGVSS
FTAVINEPEAAILAVGALVEKPVLKAGNIVPGKILNVTLSCDHRVIDGATGSRFLSLFREFMEHPLRLLTG
>Mature_470_residues
AKIAEMTQLSPTMSEGKIVRWLKQKGDSVSPGEIIAEVETDKAVMEMEAFETGVLLEILAPEGSLLPVGAPVAIIGKPGE
DVSALVEIAKKSIPAKKEGSAAPSQTSTSTQSTSSSTAPTSSAQSGTSASTSGAGAGKNLETKTSVSLSQNAGNSGITAN
EERTYGFKTSPSFGPGESEHSPVRSARGGRPIKASPLAKNLALQKGINLTEVIGSGPGGRIIKRDILSYQSGGGNTFVKR
QDRKLEITGMRKTIASRLAHSTSTIPHFYLTTELNAGPIDDLRNSINRDLGLSGQGKVSVNDLILKACSYTLLQVPEVNS
SWREDHILEHGRVDIGVAVSIEGGLITPYIRNAEEKSVLEISREIKELASRARDRKLKPGEYTDGTFTVSNLGMFGVSSF
TAVINEPEAAILAVGALVEKPVLKAGNIVPGKILNVTLSCDHRVIDGATGSRFLSLFREFMEHPLRLLTG

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=313, Percent_Identity=42.4920127795527, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI203098816, Length=227, Percent_Identity=43.6123348017621, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI203098753, Length=227, Percent_Identity=43.6123348017621, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI110671329, Length=332, Percent_Identity=31.6265060240964, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI260898739, Length=163, Percent_Identity=44.7852760736196, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=30.7359307359307, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786305, Length=306, Percent_Identity=31.3725490196078, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1786946, Length=475, Percent_Identity=25.0526315789474, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17560088, Length=471, Percent_Identity=39.9150743099788, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=34.7540983606557, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=481, Percent_Identity=23.7006237006237, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=455, Percent_Identity=25.9340659340659, Blast_Score=135, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324258, Length=498, Percent_Identity=33.7349397590361, Blast_Score=239, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6320352, Length=238, Percent_Identity=34.453781512605, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6321632, Length=85, Percent_Identity=41.1764705882353, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129315, Length=285, Percent_Identity=40.7017543859649, Blast_Score=208, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24582497, Length=221, Percent_Identity=46.1538461538462, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI18859875, Length=303, Percent_Identity=29.042904290429, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24645909, Length=232, Percent_Identity=31.0344827586207, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 49805; Mature: 49674

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIAEMTQLSPTMSEGKIVRWLKQKGDSVSPGEIIAEVETDKAVMEMEAFETGVLLEIL
CCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEEE
APEGSLLPVGAPVAIIGKPGEDVSALVEIAKKSIPAKKEGSAAPSQTSTSTQSTSSSTAP
CCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
TSSAQSGTSASTSGAGAGKNLETKTSVSLSQNAGNSGITANEERTYGFKTSPSFGPGESE
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HSPVRSARGGRPIKASPLAKNLALQKGINLTEVIGSGPGGRIIKRDILSYQSGGGNTFVK
CCCHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEE
RQDRKLEITGMRKTIASRLAHSTSTIPHFYLTTELNAGPIDDLRNSINRDLGLSGQGKVS
CCCCEEEEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEE
VNDLILKACSYTLLQVPEVNSSWREDHILEHGRVDIGVAVSIEGGLITPYIRNAEEKSVL
HHHHHHHHHHHHEEECCCCCCCCCHHHHHHCCCEEEEEEEEECCCEECHHHCCCCHHHHH
EISREIKELASRARDRKLKPGEYTDGTFTVSNLGMFGVSSFTAVINEPEAAILAVGALVE
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
KPVLKAGNIVPGKILNVTLSCDHRVIDGATGSRFLSLFREFMEHPLRLLTG
HHHHHCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKIAEMTQLSPTMSEGKIVRWLKQKGDSVSPGEIIAEVETDKAVMEMEAFETGVLLEIL
CCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEEE
APEGSLLPVGAPVAIIGKPGEDVSALVEIAKKSIPAKKEGSAAPSQTSTSTQSTSSSTAP
CCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
TSSAQSGTSASTSGAGAGKNLETKTSVSLSQNAGNSGITANEERTYGFKTSPSFGPGESE
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HSPVRSARGGRPIKASPLAKNLALQKGINLTEVIGSGPGGRIIKRDILSYQSGGGNTFVK
CCCHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEE
RQDRKLEITGMRKTIASRLAHSTSTIPHFYLTTELNAGPIDDLRNSINRDLGLSGQGKVS
CCCCEEEEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEE
VNDLILKACSYTLLQVPEVNSSWREDHILEHGRVDIGVAVSIEGGLITPYIRNAEEKSVL
HHHHHHHHHHHHEEECCCCCCCCCHHHHHHCCCEEEEEEEEECCCEECHHHCCCCHHHHH
EISREIKELASRARDRKLKPGEYTDGTFTVSNLGMFGVSSFTAVINEPEAAILAVGALVE
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
KPVLKAGNIVPGKILNVTLSCDHRVIDGATGSRFLSLFREFMEHPLRLLTG
HHHHHCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]