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The map label for this gene is topA [H]

Identifier: 116327989

GI number: 116327989

Start: 1501459

End: 1503300

Strand: Direct

Name: topA [H]

Synonym: LBL_1279

Alternate gene names: 116327989

Gene position: 1501459-1503300 (Clockwise)

Preceding gene: 116327988

Following gene: 116327992

Centisome position: 41.54

GC content: 40.83

Gene sequence:

>1842_bases
GTGTCCTCACTTATCATTGTTGAATCTCCCTCCAAAGCAAAAACTATCGGCGGCTATTTAGGCAAAGAATTTAGAATTCT
CGCAACACTCGGACACGTGGCCGATCTTCCTAAGACGACTCTCGGATTGGATCTGAAGAATCGTTTTGAGCCGGAATACG
TAGTTCTTCCCGGAAAGAAAAAGATTCTTTCCGAAATCATAAAGGCGGCAAAAGAATCCCAAAGAATTTTTCTGGCAACC
GATCCGGACCGAGAGGGAGAATTTATCAGCGCCTATATCCGGGATCGCCTGAAAAAAAAATCGAATGTCTTTCGGATTCG
TTTTACGGAGATCACGCGAAACGCGATCCTGAGTTCTTTACAAAATCCGGATACGATCCATGAATCCTTGGTTGAAGCCC
AGAAAACGAGAAGAGTCGGGGATCGGCTGATCGGTTATTTTATCAGTCCCGTTCTTTGGAGGGAGATTGGCCCTGGATTA
TCTGCCGGAAGAGTACAGTCTGTCGCTTTAAAGTGGATCTGTGACAGAGAGGACGAGATTCGAAATTTTCATTCAGAAAT
ATATTATAATGTACTATTATATGTACATGATAAAAAAGGGATCGAAGGTGTTTTCCAAAGGGTGGGCGATCGGATTTTTT
CAGAAGAAAAAGCAAATCTGATTTTACAAAACGTTCAAAAAGAAAAAAATCTCCGGATTTCTGAAAAAAAAGAATCTCAT
AAAAAAAACCTGCCTCCTCCTCCATTTCAAACGGCGAGTTTACAACAGGAAGCTTTTCGAAAATTACGATTCTCTTCTAA
AAAGACGATGAGCATTGCTCAGAAACTTTACGAAGGAATGGATCTTGGAAACGGTAAAAGAGAGGGGTTGATCACATATA
TGAGAACGGATTCCGTTCGTTTGAGTCCGGATTTTGTGGAACGGGCAAACTCTTGGATCATTTCCGAGTTTGGTGAAACT
TTTGCGAACAAACTGGAACGTAAAGTCAAAAAATCTAGTAAGAAGATTCAGGATGCACACGAAGCGATCCGTATTACGGA
TCCGTTTTTAACTTCCGAAAGCGCAAAAAATTTTTTAGGAAAGGAAGAAGCTTCTCTTTATGAGCTGATCTGGAAACGAA
CCATTTCGTATCTTCTACCTCCGGAAGAATTCTTAAACACTGAATATTCCATTTTTGTGGCCGGAGAATATTTTCAATTG
GAAACTAAAAAGACTCTCTTTCCCGGATATAAAATATTAAACGAAGCGGATAAAAAGGCGAATCCAAATTGGGAAAAAGG
AGAGCTGTTGACCCTTCAGAAAGCGGAGTGTGAAAAAAAACAAACGGAACCGTCTCCTCGATACTCGGAGGGCACTCTTG
TCGCAAAACTGGAAAGGGAAGGGATTGGCCGGCCTTCCACATACTCAACGGTATCGGAGATACTTGTTAAAAGGAAATAC
GTCGAACAGGAAAAGAAATTTTTTTATCCGCTTCCATTGGGAGAAAAGGTCAACTTTTTTCTACAATCCGGTTTCGGAGA
TCTATTTCGGGAAAAATTTACCGCGGAACTCGAATCAAATTTGGATAGGATCGAAAAAAACGAGATCGATTCTCTTGTCA
TATTAAATCGACTTTGGTCCGATCTGCAAACTCAGATTCAGAATAGCAAGTTTGTCGCTTCCGTGTTTCGAAAAGAATGG
GCGGAGATTCGAGAAAAGAAAAAAGAAACGAGTTGGGGGATTTGTCCTCTTTGTAGGGATGGTTCTCTCCAAAAGAAAAA
AACGTCTCGAAAAAAGGAATTTTATCAATGTAGTCGCTTTCCCGATTGTGAATATGTAAACTACGAGCTCCCTAAGCCTT
AA

Upstream 100 bases:

>100_bases
TTGCTTTTCCCCGGAGAAATTCAAAATCCGGGGTATTCTTTTTTTTATATTTAGCGATATTTAATTGAAAATCTGAAACT
AAACTAAACCTTAAGAATTG

Downstream 100 bases:

>100_bases
AATTTTCTCTATCGTATTTTGCGAAACTTCAATTTTATAAAGAGCCCGGCCCGAGCCTGAAAGTGAGAACTCTTACAGGA
ATAGGACAACAAGAAAAGGT

Product: DNA topoisomerase

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MSSLIIVESPSKAKTIGGYLGKEFRILATLGHVADLPKTTLGLDLKNRFEPEYVVLPGKKKILSEIIKAAKESQRIFLAT
DPDREGEFISAYIRDRLKKKSNVFRIRFTEITRNAILSSLQNPDTIHESLVEAQKTRRVGDRLIGYFISPVLWREIGPGL
SAGRVQSVALKWICDREDEIRNFHSEIYYNVLLYVHDKKGIEGVFQRVGDRIFSEEKANLILQNVQKEKNLRISEKKESH
KKNLPPPPFQTASLQQEAFRKLRFSSKKTMSIAQKLYEGMDLGNGKREGLITYMRTDSVRLSPDFVERANSWIISEFGET
FANKLERKVKKSSKKIQDAHEAIRITDPFLTSESAKNFLGKEEASLYELIWKRTISYLLPPEEFLNTEYSIFVAGEYFQL
ETKKTLFPGYKILNEADKKANPNWEKGELLTLQKAECEKKQTEPSPRYSEGTLVAKLEREGIGRPSTYSTVSEILVKRKY
VEQEKKFFYPLPLGEKVNFFLQSGFGDLFREKFTAELESNLDRIEKNEIDSLVILNRLWSDLQTQIQNSKFVASVFRKEW
AEIREKKKETSWGICPLCRDGSLQKKKTSRKKEFYQCSRFPDCEYVNYELPKP

Sequences:

>Translated_613_residues
MSSLIIVESPSKAKTIGGYLGKEFRILATLGHVADLPKTTLGLDLKNRFEPEYVVLPGKKKILSEIIKAAKESQRIFLAT
DPDREGEFISAYIRDRLKKKSNVFRIRFTEITRNAILSSLQNPDTIHESLVEAQKTRRVGDRLIGYFISPVLWREIGPGL
SAGRVQSVALKWICDREDEIRNFHSEIYYNVLLYVHDKKGIEGVFQRVGDRIFSEEKANLILQNVQKEKNLRISEKKESH
KKNLPPPPFQTASLQQEAFRKLRFSSKKTMSIAQKLYEGMDLGNGKREGLITYMRTDSVRLSPDFVERANSWIISEFGET
FANKLERKVKKSSKKIQDAHEAIRITDPFLTSESAKNFLGKEEASLYELIWKRTISYLLPPEEFLNTEYSIFVAGEYFQL
ETKKTLFPGYKILNEADKKANPNWEKGELLTLQKAECEKKQTEPSPRYSEGTLVAKLEREGIGRPSTYSTVSEILVKRKY
VEQEKKFFYPLPLGEKVNFFLQSGFGDLFREKFTAELESNLDRIEKNEIDSLVILNRLWSDLQTQIQNSKFVASVFRKEW
AEIREKKKETSWGICPLCRDGSLQKKKTSRKKEFYQCSRFPDCEYVNYELPKP
>Mature_612_residues
SSLIIVESPSKAKTIGGYLGKEFRILATLGHVADLPKTTLGLDLKNRFEPEYVVLPGKKKILSEIIKAAKESQRIFLATD
PDREGEFISAYIRDRLKKKSNVFRIRFTEITRNAILSSLQNPDTIHESLVEAQKTRRVGDRLIGYFISPVLWREIGPGLS
AGRVQSVALKWICDREDEIRNFHSEIYYNVLLYVHDKKGIEGVFQRVGDRIFSEEKANLILQNVQKEKNLRISEKKESHK
KNLPPPPFQTASLQQEAFRKLRFSSKKTMSIAQKLYEGMDLGNGKREGLITYMRTDSVRLSPDFVERANSWIISEFGETF
ANKLERKVKKSSKKIQDAHEAIRITDPFLTSESAKNFLGKEEASLYELIWKRTISYLLPPEEFLNTEYSIFVAGEYFQLE
TKKTLFPGYKILNEADKKANPNWEKGELLTLQKAECEKKQTEPSPRYSEGTLVAKLEREGIGRPSTYSTVSEILVKRKYV
EQEKKFFYPLPLGEKVNFFLQSGFGDLFREKFTAELESNLDRIEKNEIDSLVILNRLWSDLQTQIQNSKFVASVFRKEWA
EIREKKKETSWGICPLCRDGSLQKKKTSRKKEFYQCSRFPDCEYVNYELPKP

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=583, Percent_Identity=24.8713550600343, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI4507635, Length=495, Percent_Identity=23.2323232323232, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1787529, Length=640, Percent_Identity=32.65625, Blast_Score=319, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI17555378, Length=502, Percent_Identity=24.1035856573705, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32563869, Length=557, Percent_Identity=22.6211849192101, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585251, Length=575, Percent_Identity=24, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 70969; Mature: 70838

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLIIVESPSKAKTIGGYLGKEFRILATLGHVADLPKTTLGLDLKNRFEPEYVVLPGKK
CCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHCCCHHHCCCCCEEEECCHH
KILSEIIKAAKESQRIFLATDPDREGEFISAYIRDRLKKKSNVFRIRFTEITRNAILSSL
HHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHC
QNPDTIHESLVEAQKTRRVGDRLIGYFISPVLWREIGPGLSAGRVQSVALKWICDREDEI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHH
RNFHSEIYYNVLLYVHDKKGIEGVFQRVGDRIFSEEKANLILQNVQKEKNLRISEKKESH
HHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KKNLPPPPFQTASLQQEAFRKLRFSSKKTMSIAQKLYEGMDLGNGKREGLITYMRTDSVR
CCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEE
LSPDFVERANSWIISEFGETFANKLERKVKKSSKKIQDAHEAIRITDPFLTSESAKNFLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHCC
KEEASLYELIWKRTISYLLPPEEFLNTEYSIFVAGEYFQLETKKTLFPGYKILNEADKKA
CHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCEEEECCCHHHCCCHHHHHHHHHCC
NPNWEKGELLTLQKAECEKKQTEPSPRYSEGTLVAKLEREGIGRPSTYSTVSEILVKRKY
CCCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHHH
VEQEKKFFYPLPLGEKVNFFLQSGFGDLFREKFTAELESNLDRIEKNEIDSLVILNRLWS
HHHHHCEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLQTQIQNSKFVASVFRKEWAEIREKKKETSWGICPLCRDGSLQKKKTSRKKEFYQCSRF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PDCEYVNYELPKP
CCCCCCCCCCCCC
>Mature Secondary Structure 
SSLIIVESPSKAKTIGGYLGKEFRILATLGHVADLPKTTLGLDLKNRFEPEYVVLPGKK
CCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHCCCHHHCCCCCEEEECCHH
KILSEIIKAAKESQRIFLATDPDREGEFISAYIRDRLKKKSNVFRIRFTEITRNAILSSL
HHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHC
QNPDTIHESLVEAQKTRRVGDRLIGYFISPVLWREIGPGLSAGRVQSVALKWICDREDEI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHH
RNFHSEIYYNVLLYVHDKKGIEGVFQRVGDRIFSEEKANLILQNVQKEKNLRISEKKESH
HHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KKNLPPPPFQTASLQQEAFRKLRFSSKKTMSIAQKLYEGMDLGNGKREGLITYMRTDSVR
CCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEE
LSPDFVERANSWIISEFGETFANKLERKVKKSSKKIQDAHEAIRITDPFLTSESAKNFLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHCC
KEEASLYELIWKRTISYLLPPEEFLNTEYSIFVAGEYFQLETKKTLFPGYKILNEADKKA
CHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCEEEECCCHHHCCCHHHHHHHHHCC
NPNWEKGELLTLQKAECEKKQTEPSPRYSEGTLVAKLEREGIGRPSTYSTVSEILVKRKY
CCCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHHH
VEQEKKFFYPLPLGEKVNFFLQSGFGDLFREKFTAELESNLDRIEKNEIDSLVILNRLWS
HHHHHCEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLQTQIQNSKFVASVFRKEWAEIREKKKETSWGICPLCRDGSLQKKKTSRKKEFYQCSRF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PDCEYVNYELPKP
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]