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The map label for this gene is cysK-2 [H]

Identifier: 116327988

GI number: 116327988

Start: 1500404

End: 1501333

Strand: Direct

Name: cysK-2 [H]

Synonym: LBL_1278

Alternate gene names: 116327988

Gene position: 1500404-1501333 (Clockwise)

Preceding gene: 116327987

Following gene: 116327989

Centisome position: 41.51

GC content: 43.01

Gene sequence:

>930_bases
ATGAAAGCTGAGAGTATTTTAGAAACGATCGGAAATACACCCCATGTAAAAATTAATCGCTTGTTTAAAACAAAGAGCGA
GGTTTATGCAAAATTAGAAAGATCAAACCCAGGCGGATCTATAAAAGATCGAATTGCTCTTTCCATGATTGAGGATGCGG
AAAAAACCGGAAAACTCACTAAGGATAGTATCATCGTAGAACCTACTTCCGGAAACACTGGAATCGGTTTGGCTCTTGTT
GCCGCTGTGAAGGGTTACAAACTTCTTTTAGTAATGCCTGAATCCATGAGCGTGGAAAGAAGAAGAATTATGGCCGCTTA
TGGAGCTGAATTCGAACTAACTCCGAGAGAAAAAGGAATGCCGGGAGCGATTGAAAAAGCAAAACAAATCGTGGCAGAAA
ACCCAAAAGCTTGGATGCCGCAACAGTTTGAGAACGAAGCGAATATCAAAGTTCACGTAGAAACGACAGCCCAAGAAATT
GCAAAAGATTTTCCGGACGGACTAGACTACATAATCACAGGGGTTGGAACTGGAGGACATATCACAGGTGTAGCTCAGGT
TTTGAAGCAAAAATTTCCAAAGTTGAAAGTGTTTGCTGTCGAGCCGGAAGCGTCTCCCGTAATCTCGGGAGGAAAACCTG
GGCCGCACCCGATTCAAGGAATCGGAGCTGGGTTCATTCCTAAAAACCTTCACACGAATCTTTTAGACGGAACCATTCAA
ATCAGTAAAGAAGAAGCATTCGAATACGCACAAAGAGCGGCAAAGGAAGAAGGGCTTTTTATCGGAGTTTCTTCCGGCGC
AGCGCTTGCAGCAGTTGCAAAAAAATTACCGGAAATTCCCGAAGGTTCGAAAGTATTAACTTTTTCTTATGATACAGGAG
AAAGATATCTTTCGATCGAAGGACTTTTCCCGGTTCCTGCGAACGCATAA

Upstream 100 bases:

>100_bases
AAAGGATTTTTTTCTTGAACCTATTTGCTATATTGAAATAAATGTCTAATATAACAACATCTAAACTCAAGAATCCTTAT
TATGAGGCAGGAAAACAGAA

Downstream 100 bases:

>100_bases
AAAGTTTTTTCTGATCGAAATCTTTTTGCTTTTCCCCGGAGAAATTCAAAATCCGGGGTATTCTTTTTTTTATATTTAGC
GATATTTAATTGAAAATCTG

Product: cysteine synthase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKAESILETIGNTPHVKINRLFKTKSEVYAKLERSNPGGSIKDRIALSMIEDAEKTGKLTKDSIIVEPTSGNTGIGLALV
AAVKGYKLLLVMPESMSVERRRIMAAYGAEFELTPREKGMPGAIEKAKQIVAENPKAWMPQQFENEANIKVHVETTAQEI
AKDFPDGLDYIITGVGTGGHITGVAQVLKQKFPKLKVFAVEPEASPVISGGKPGPHPIQGIGAGFIPKNLHTNLLDGTIQ
ISKEEAFEYAQRAAKEEGLFIGVSSGAALAAVAKKLPEIPEGSKVLTFSYDTGERYLSIEGLFPVPANA

Sequences:

>Translated_309_residues
MKAESILETIGNTPHVKINRLFKTKSEVYAKLERSNPGGSIKDRIALSMIEDAEKTGKLTKDSIIVEPTSGNTGIGLALV
AAVKGYKLLLVMPESMSVERRRIMAAYGAEFELTPREKGMPGAIEKAKQIVAENPKAWMPQQFENEANIKVHVETTAQEI
AKDFPDGLDYIITGVGTGGHITGVAQVLKQKFPKLKVFAVEPEASPVISGGKPGPHPIQGIGAGFIPKNLHTNLLDGTIQ
ISKEEAFEYAQRAAKEEGLFIGVSSGAALAAVAKKLPEIPEGSKVLTFSYDTGERYLSIEGLFPVPANA
>Mature_309_residues
MKAESILETIGNTPHVKINRLFKTKSEVYAKLERSNPGGSIKDRIALSMIEDAEKTGKLTKDSIIVEPTSGNTGIGLALV
AAVKGYKLLLVMPESMSVERRRIMAAYGAEFELTPREKGMPGAIEKAKQIVAENPKAWMPQQFENEANIKVHVETTAQEI
AKDFPDGLDYIITGVGTGGHITGVAQVLKQKFPKLKVFAVEPEASPVISGGKPGPHPIQGIGAGFIPKNLHTNLLDGTIQ
ISKEEAFEYAQRAAKEEGLFIGVSSGAALAAVAKKLPEIPEGSKVLTFSYDTGERYLSIEGLFPVPANA

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=305, Percent_Identity=40, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI295821200, Length=305, Percent_Identity=40, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI4557415, Length=305, Percent_Identity=40, Blast_Score=217, Evalue=1e-56,
Organism=Escherichia coli, GI1788754, Length=300, Percent_Identity=49.6666666666667, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI2367138, Length=300, Percent_Identity=39.3333333333333, Blast_Score=195, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17535051, Length=295, Percent_Identity=42.7118644067797, Blast_Score=225, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI115535073, Length=293, Percent_Identity=48.122866894198, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17562970, Length=297, Percent_Identity=41.7508417508418, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI32566674, Length=294, Percent_Identity=42.1768707482993, Blast_Score=221, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17561720, Length=297, Percent_Identity=41.4141414141414, Blast_Score=197, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17534315, Length=313, Percent_Identity=38.9776357827476, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI25147552, Length=279, Percent_Identity=38.3512544802867, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI32566672, Length=211, Percent_Identity=40.2843601895735, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17561716, Length=117, Percent_Identity=38.4615384615385, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=35.5555555555556, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321449, Length=332, Percent_Identity=34.9397590361446, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24643623, Length=305, Percent_Identity=36.0655737704918, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI20129101, Length=305, Percent_Identity=36.0655737704918, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33179; Mature: 33179

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00901 CYS_SYNTHASE ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAESILETIGNTPHVKINRLFKTKSEVYAKLERSNPGGSIKDRIALSMIEDAEKTGKLT
CCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
KDSIIVEPTSGNTGIGLALVAAVKGYKLLLVMPESMSVERRRIMAAYGAEFELTPREKGM
CCCEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCC
PGAIEKAKQIVAENPKAWMPQQFENEANIKVHVETTAQEIAKDFPDGLDYIITGVGTGGH
CHHHHHHHHHHHCCCCCCCCHHHCCCCCEEEEEECHHHHHHHHCCCCCEEEEEECCCCCH
ITGVAQVLKQKFPKLKVFAVEPEASPVISGGKPGPHPIQGIGAGFIPKNLHTNLLDGTIQ
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEEE
ISKEEAFEYAQRAAKEEGLFIGVSSGAALAAVAKKLPEIPEGSKVLTFSYDTGERYLSIE
ECHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEE
GLFPVPANA
CCCCCCCCC
>Mature Secondary Structure
MKAESILETIGNTPHVKINRLFKTKSEVYAKLERSNPGGSIKDRIALSMIEDAEKTGKLT
CCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
KDSIIVEPTSGNTGIGLALVAAVKGYKLLLVMPESMSVERRRIMAAYGAEFELTPREKGM
CCCEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCC
PGAIEKAKQIVAENPKAWMPQQFENEANIKVHVETTAQEIAKDFPDGLDYIITGVGTGGH
CHHHHHHHHHHHCCCCCCCCHHHCCCCCEEEEEECHHHHHHHHCCCCCEEEEEECCCCCH
ITGVAQVLKQKFPKLKVFAVEPEASPVISGGKPGPHPIQGIGAGFIPKNLHTNLLDGTIQ
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEEE
ISKEEAFEYAQRAAKEEGLFIGVSSGAALAAVAKKLPEIPEGSKVLTFSYDTGERYLSIE
ECHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEE
GLFPVPANA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867384 [H]