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The map label for this gene is gapA [H]

Identifier: 116327982

GI number: 116327982

Start: 1493281

End: 1494288

Strand: Reverse

Name: gapA [H]

Synonym: LBL_1271

Alternate gene names: 116327982

Gene position: 1494288-1493281 (Counterclockwise)

Preceding gene: 116327984

Following gene: 116327981

Centisome position: 41.34

GC content: 46.63

Gene sequence:

>1008_bases
ATGACTAAAATAGCCATCAACGGATTTGGAAGAATCGGAAGACTCGTTTTCCGTGCAGGAATCAAAGACCCTAATCTGGA
ATTTGTCGCAATCAATGATTTAGTTACTCCTGACAATCTGGCCTACTTACTCAAATACGATTCCACTCATGGAAAATTCC
AAGGGACCGTCGAACATACCGATAAAGAACTGATCGTAGACGGAAAAAAAGTTCTCTGCGTTTCCGAAAGAGATCCTGAA
AAACTCCCCTGGAAAGACCTGAAAGTGGACTACGTGATCGAATCCACCGGACTTTTTACCGACAGAACGGGAGCAGAAAA
ACACATCAAAGCGGGAGCGAAAAAAGTCGTAATTTCCGCGCCTGCAAAAGACAAGGATATCCCCACATTCGTAATGGGCG
TCAACAACGAGCAATACAACGCGGCAACCGATCACGTTGTGTCTAACGCGTCTTGCACTACAAATTGCCTGGCCCCGATC
ACTAAAGTCGTCCTTGATAACTTTGGGATCGAAGAAGGCCTAATGACCACGATCCACGCAACAACGGCGACTCAGCCTAC
CGTAGACGGTCCTTCCAAAAAAGACTTCCGCGGCGGAAGGGGAGCAATGCAAAATATTATCCCTGCGGCGACCGGCGCTG
CAAAAGCGGTGGGACTTTGTATTCCTGAAGTTAACGGCAAACTGACAGGTATGTCTTTCCGTGTTCCGACTCCGGACGTT
TCCGTCGTCGACTTAACCGTTAGAACCACGAAAGAAACCTCTCTGAAAGAAATTTCCGCAAAGATGAAAGAAGCTTCCGA
AGGATCCATGAAAGGAATCCTCGGTTATACGAAAGACATGGTTGTTTCCAACGACTTCGTAAGTTCCACACTCTCCTCCA
TTTTCGATCAAGACGCTTGTATAGAATTGAACTCCAGATTCTTTAAGCTCGTTTCCTGGTATGACAACGAGATGGGATAC
TCCAACAGAGTTCTAGACCTCATCCGCTATATGGCGAAGAAAGGCTAG

Upstream 100 bases:

>100_bases
GAATTATCCGGGAAAATACTGTACCTTAAAAAATTCTCCCCTACGCACATCGCCGAACAGGGAGTTCTAAAATAAAATCT
GAAACAACAAAGGGAAAAAA

Downstream 100 bases:

>100_bases
TTCATGGAATTGCCTAGACTCGAAAACGTCGACCTCTCGGGAAAAAGAGTTTTTCTGAGGGTAGACTTCAACGTTCCCAT
AGAAAACGGAAAAGTTACCG

Product: glyceraldehyde 3-phosphate dehydrogenase (phosphorylating)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTKIAINGFGRIGRLVFRAGIKDPNLEFVAINDLVTPDNLAYLLKYDSTHGKFQGTVEHTDKELIVDGKKVLCVSERDPE
KLPWKDLKVDYVIESTGLFTDRTGAEKHIKAGAKKVVISAPAKDKDIPTFVMGVNNEQYNAATDHVVSNASCTTNCLAPI
TKVVLDNFGIEEGLMTTIHATTATQPTVDGPSKKDFRGGRGAMQNIIPAATGAAKAVGLCIPEVNGKLTGMSFRVPTPDV
SVVDLTVRTTKETSLKEISAKMKEASEGSMKGILGYTKDMVVSNDFVSSTLSSIFDQDACIELNSRFFKLVSWYDNEMGY
SNRVLDLIRYMAKKG

Sequences:

>Translated_335_residues
MTKIAINGFGRIGRLVFRAGIKDPNLEFVAINDLVTPDNLAYLLKYDSTHGKFQGTVEHTDKELIVDGKKVLCVSERDPE
KLPWKDLKVDYVIESTGLFTDRTGAEKHIKAGAKKVVISAPAKDKDIPTFVMGVNNEQYNAATDHVVSNASCTTNCLAPI
TKVVLDNFGIEEGLMTTIHATTATQPTVDGPSKKDFRGGRGAMQNIIPAATGAAKAVGLCIPEVNGKLTGMSFRVPTPDV
SVVDLTVRTTKETSLKEISAKMKEASEGSMKGILGYTKDMVVSNDFVSSTLSSIFDQDACIELNSRFFKLVSWYDNEMGY
SNRVLDLIRYMAKKG
>Mature_334_residues
TKIAINGFGRIGRLVFRAGIKDPNLEFVAINDLVTPDNLAYLLKYDSTHGKFQGTVEHTDKELIVDGKKVLCVSERDPEK
LPWKDLKVDYVIESTGLFTDRTGAEKHIKAGAKKVVISAPAKDKDIPTFVMGVNNEQYNAATDHVVSNASCTTNCLAPIT
KVVLDNFGIEEGLMTTIHATTATQPTVDGPSKKDFRGGRGAMQNIIPAATGAAKAVGLCIPEVNGKLTGMSFRVPTPDVS
VVDLTVRTTKETSLKEISAKMKEASEGSMKGILGYTKDMVVSNDFVSSTLSSIFDQDACIELNSRFFKLVSWYDNEMGYS
NRVLDLIRYMAKKG

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=333, Percent_Identity=58.8588588588589, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI7657116, Length=329, Percent_Identity=56.5349544072948, Blast_Score=386, Evalue=1e-107,
Organism=Escherichia coli, GI1788079, Length=331, Percent_Identity=63.7462235649547, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1789295, Length=333, Percent_Identity=39.039039039039, Blast_Score=241, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI17534677, Length=338, Percent_Identity=62.4260355029586, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17534679, Length=338, Percent_Identity=62.1301775147929, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32566163, Length=342, Percent_Identity=61.9883040935673, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17568413, Length=337, Percent_Identity=62.0178041543027, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6321631, Length=334, Percent_Identity=61.6766467065868, Blast_Score=421, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322409, Length=334, Percent_Identity=60.1796407185629, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322468, Length=334, Percent_Identity=60.7784431137725, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI85725000, Length=335, Percent_Identity=62.089552238806, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI22023983, Length=335, Percent_Identity=62.089552238806, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17933600, Length=335, Percent_Identity=61.4925373134328, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI18110149, Length=335, Percent_Identity=61.4925373134328, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI19922412, Length=331, Percent_Identity=55.8912386706949, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36514; Mature: 36383

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIAINGFGRIGRLVFRAGIKDPNLEFVAINDLVTPDNLAYLLKYDSTHGKFQGTVEHT
CCEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEEEHCCC
DKELIVDGKKVLCVSERDPEKLPWKDLKVDYVIESTGLFTDRTGAEKHIKAGAKKVVISA
CCEEEECCCEEEEECCCCCCCCCCCCCEEEEEEECCCCEECCCCCHHHHHCCCCEEEEEC
PAKDKDIPTFVMGVNNEQYNAATDHVVSNASCTTNCLAPITKVVLDNFGIEEGLMTTIHA
CCCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEE
TTATQPTVDGPSKKDFRGGRGAMQNIIPAATGAAKAVGLCIPEVNGKLTGMSFRVPTPDV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHEEEEEEECCCCEEEEEEEECCCCCC
SVVDLTVRTTKETSLKEISAKMKEASEGSMKGILGYTKDMVVSNDFVSSTLSSIFDQDAC
EEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHEECCHHHHHHHHHHHCCCCE
IELNSRFFKLVSWYDNEMGYSNRVLDLIRYMAKKG
EHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKIAINGFGRIGRLVFRAGIKDPNLEFVAINDLVTPDNLAYLLKYDSTHGKFQGTVEHT
CEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEEEHCCC
DKELIVDGKKVLCVSERDPEKLPWKDLKVDYVIESTGLFTDRTGAEKHIKAGAKKVVISA
CCEEEECCCEEEEECCCCCCCCCCCCCEEEEEEECCCCEECCCCCHHHHHCCCCEEEEEC
PAKDKDIPTFVMGVNNEQYNAATDHVVSNASCTTNCLAPITKVVLDNFGIEEGLMTTIHA
CCCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEE
TTATQPTVDGPSKKDFRGGRGAMQNIIPAATGAAKAVGLCIPEVNGKLTGMSFRVPTPDV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHEEEEEEECCCCEEEEEEEECCCCCC
SVVDLTVRTTKETSLKEISAKMKEASEGSMKGILGYTKDMVVSNDFVSSTLSSIFDQDAC
EEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHEECCHHHHHHHHHHHCCCCE
IELNSRFFKLVSWYDNEMGYSNRVLDLIRYMAKKG
EHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8378350 [H]