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The map label for this gene is tpiA

Identifier: 116327980

GI number: 116327980

Start: 1491099

End: 1491851

Strand: Reverse

Name: tpiA

Synonym: LBL_1269

Alternate gene names: 116327980

Gene position: 1491851-1491099 (Counterclockwise)

Preceding gene: 116327981

Following gene: 116327979

Centisome position: 41.27

GC content: 42.63

Gene sequence:

>753_bases
ATGCGCAAAACGGTAATCGCCGGAAACTGGAAAATGAATCTCTCCGAAAAAGAGGCTCTATCTCTGGCACATTCGATTAA
AGAAAAGATCCCTGCCATTTCTAAAGGTAGAATTTCTATGATTTTTCCTTCGACATTGCATCTTGCGGGTGTTGCGAAAA
TTTTACAAGGAACAGAAATTCTAGTCGGAGCGCAGAATGTGTATCCTTCCGGGCTTGCGGCCTTTACCGGTGAAACCTCA
CCGGAACAACTAAAAGAACTCGGCGTGAAGGTCGTTATGATCGGACATTCGGAAAGAAGACAATTTCTAGGCGAGACGAA
TTCCTTTTGTAACGAAAAGATTCATTTCTTATTAAAGAACGATTTTATCGTACTCTATTGTGTAGGCGAAACTCTTATGG
AAAGAGAATCCGGAAAAACCTTCGAAGTAATTTCTTCCCAAATTCGAGAAGGTTTGAAAGGAATCCACAGTCATTCTTTT
TCCAATCTGATCCTAGCTTATGAGCCGGTCTGGGCGATCGGAACCGGAAAAGTGGCAACACCGGCACAAGCACAAGAGGT
ACACTTTTTTATTCGTAAGGAAATAGCCGGTCTATTCTTAGGTGCGAAAGAAATTGCGGAATCCATCTCAATTCTATACG
GAGGTTCCGTAAAACCGGATAACATACAAACGCTCTTAAAGGAAAAAGATCTAGACGGCGGGCTCGTAGGAGGAGCCAGT
CAAAAAATAGATACTTACGCGGGTTTGTTCTGA

Upstream 100 bases:

>100_bases
ATCATTAGAAATGGATCGCTCTAAAAAATCCTTGAAGCAATTCCCCTTTCTTACAAACTTTCCCCAGCTTTATCGGAATC
ATCCATCAAGAGGAAATCGC

Downstream 100 bases:

>100_bases
GAACTGATCCAAAATCTCATACGCAAGAACTTTTATGAGAGTTTAACGACGAAAAAATCGTTCCAAAACGATCAAGGGAA
CGCGGACTTATTACGAACTT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MRKTVIAGNWKMNLSEKEALSLAHSIKEKIPAISKGRISMIFPSTLHLAGVAKILQGTEILVGAQNVYPSGLAAFTGETS
PEQLKELGVKVVMIGHSERRQFLGETNSFCNEKIHFLLKNDFIVLYCVGETLMERESGKTFEVISSQIREGLKGIHSHSF
SNLILAYEPVWAIGTGKVATPAQAQEVHFFIRKEIAGLFLGAKEIAESISILYGGSVKPDNIQTLLKEKDLDGGLVGGAS
QKIDTYAGLF

Sequences:

>Translated_250_residues
MRKTVIAGNWKMNLSEKEALSLAHSIKEKIPAISKGRISMIFPSTLHLAGVAKILQGTEILVGAQNVYPSGLAAFTGETS
PEQLKELGVKVVMIGHSERRQFLGETNSFCNEKIHFLLKNDFIVLYCVGETLMERESGKTFEVISSQIREGLKGIHSHSF
SNLILAYEPVWAIGTGKVATPAQAQEVHFFIRKEIAGLFLGAKEIAESISILYGGSVKPDNIQTLLKEKDLDGGLVGGAS
QKIDTYAGLF
>Mature_250_residues
MRKTVIAGNWKMNLSEKEALSLAHSIKEKIPAISKGRISMIFPSTLHLAGVAKILQGTEILVGAQNVYPSGLAAFTGETS
PEQLKELGVKVVMIGHSERRQFLGETNSFCNEKIHFLLKNDFIVLYCVGETLMERESGKTFEVISSQIREGLKGIHSHSF
SNLILAYEPVWAIGTGKVATPAQAQEVHFFIRKEIAGLFLGAKEIAESISILYGGSVKPDNIQTLLKEKDLDGGLVGGAS
QKIDTYAGLF

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=242, Percent_Identity=40.0826446280992, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI226529917, Length=242, Percent_Identity=40.0826446280992, Blast_Score=158, Evalue=4e-39,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=42.1052631578947, Blast_Score=190, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=41.7004048582996, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=40.2439024390244, Blast_Score=169, Evalue=3e-43,
Organism=Drosophila melanogaster, GI28572008, Length=241, Percent_Identity=42.3236514522822, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI28572006, Length=241, Percent_Identity=42.3236514522822, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI28572004, Length=241, Percent_Identity=42.3236514522822, Blast_Score=180, Evalue=7e-46,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_LEPBJ (Q04TF0)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800578.1
- ProteinModelPortal:   Q04TF0
- SMR:   Q04TF0
- STRING:   Q04TF0
- GeneID:   4411064
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1218
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q04TF0
- ProtClustDB:   PRK00042
- BioCyc:   LBOR355277:LBJ_1218-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27248; Mature: 27248

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 96-96 ACT_SITE 168-168 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTVIAGNWKMNLSEKEALSLAHSIKEKIPAISKGRISMIFPSTLHLAGVAKILQGTEI
CCCEEEECCEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCEE
LVGAQNVYPSGLAAFTGETSPEQLKELGVKVVMIGHSERRQFLGETNSFCNEKIHFLLKN
EEECCCCCCCCCEEECCCCCHHHHHHCCEEEEEECCHHHHHHHCCHHHHHHHHHEEEEEC
DFIVLYCVGETLMERESGKTFEVISSQIREGLKGIHSHSFSNLILAYEPVWAIGTGKVAT
CEEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCC
PAQAQEVHFFIRKEIAGLFLGAKEIAESISILYGGSVKPDNIQTLLKEKDLDGGLVGGAS
CCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEECCCC
QKIDTYAGLF
HHHHHHHCCC
>Mature Secondary Structure
MRKTVIAGNWKMNLSEKEALSLAHSIKEKIPAISKGRISMIFPSTLHLAGVAKILQGTEI
CCCEEEECCEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCEE
LVGAQNVYPSGLAAFTGETSPEQLKELGVKVVMIGHSERRQFLGETNSFCNEKIHFLLKN
EEECCCCCCCCCEEECCCCCHHHHHHCCEEEEEECCHHHHHHHCCHHHHHHHHHEEEEEC
DFIVLYCVGETLMERESGKTFEVISSQIREGLKGIHSHSFSNLILAYEPVWAIGTGKVAT
CEEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCC
PAQAQEVHFFIRKEIAGLFLGAKEIAESISILYGGSVKPDNIQTLLKEKDLDGGLVGGAS
CCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEECCCC
QKIDTYAGLF
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA