Click here to switch to the map view.

The map label for this gene is ilvB [H]

Identifier: 116327855

GI number: 116327855

Start: 1337814

End: 1339523

Strand: Direct

Name: ilvB [H]

Synonym: LBL_1133

Alternate gene names: 116327855

Gene position: 1337814-1339523 (Clockwise)

Preceding gene: 116327851

Following gene: 116327856

Centisome position: 37.01

GC content: 45.5

Gene sequence:

>1710_bases
ATGTCTGCAGAATCAGAAAGAATAACGGGTGCCAGACTGATGGTGGAACTTCTGGAAGGATATGGTGTGGATGTCGTTTT
CGGATATCCTGGCGGGGCTATACTTCCTTTTTACGACGAGATTTATAAAAGTAAAAAAATCAAACATATCCTCGTAAGAC
ACGAACAAGGTGCGGTTCATATGGCGGAAGGATACGCGAGAGCCACCGGAAAACTTGGAGTGTGCATCGCAACATCGGGG
CCGGGGGCGACGAACTTGGTCACCGGACTTACAGATGCGAAGATGGATTCGGTTCCTATTCTCGCGATTACGGGGCAAGT
TGCGACAGATGCGATCGGTACGGATGCTTTTCAAGAAGCTGATATTTTCGGAATCACAATTCCCATCACGAAGTACAATG
CACTCATAAAGTCTGCGAATGATATTGCAAGACACTTTGAGGAGGCGACCTTGATTGCGTTAGGTGGTCGTCCGGGGCCG
GTTCTGCTTGATTTTCCGAAGGACGTTCAAACTGGGTTGACCGACGTAAGAAAGGCGACCCGTCTGAAGATTGCCCCTCA
TCATTATAAAAAACCGGAAGTTCGTGGAAATTTGGAAGAGTTTGCGGGGGCAATCAACCGATCCAAAAAGCCTCTTCTTT
ATGTGGGAGGAGGAGCGATCAATGCGGTCGCTTACAAGGAAATTTATGAACTTGCGACCAAGGCCCAAATTCCGGTCACC
ACAACCTTGATGGGACTCGGTTCCTTTCCCGGAACACATTCCTTAAGCATGGGGATGCTCGGAATGCATGGGACCGTAGC
CGCGAATAAAGCGGTATTAGAATGTGATTATATACTCAATCTGGGCGCAAGATTTGACGACCGTGTGGCAAAAATCGGGG
AGTTTGCGGAAAATGCGGTCAGAGCGCATATAGATATTGATACGGCAGAATTTAATAAACGAGTTCAAGTGGATCATCTG
TTGCACGGGGATTTGAAAGATACATTGAGAGCCCTCATACCATTCGTACAAAAACGGGATCGTACTTCCTGGATTCAATA
TCTCCAAGGTTTAAAAAAGGACCATCCGTTGGATTTCGATAACTCCGGAAATGCGATAAAACCTCAGGATTTCCTAGAGC
GTCTCTATAAAAAAACTCAAGGCAAAGCGATCGTTTCCACGGATGTAGGACAACATCAAATGTGGACCGCTCAATATTAT
CTTTTGGACGATCCAAACAATTGGCTAACTTCCGGAGGTCTCGGAACCATGGGATACGGACTCCCTGCCGCGATCGGAGC
AAAGTTTGGAAGGCCCGATAAGATGACGATCTGCGTATCCGGTGACGGGTCGATTCAAATGAATATTCAAGAACTTGCAA
CTATTGCCGCGAACAAATTGGGAGTGAAAATTCTAGTGTTCAATAATAACTTTCTTGGAATGGTTCGCCAGTGGCAGGAA
TTATTTTACGAAGAGCGATTTTCCCAATCCGAATGGAATTTTAACCCGGACTTTGTAAAACTCGCGGAGGCTTATTCGAT
TCCTGCAATGAAAATTTCGGATAAATCGGAAATAGACCGGGCAATCGAATTCTTTACAAAAGATGACGGGGCGGCATTTT
TAGAAGTGATCATACCGGCGGAAGAAAAGGTATTTCCGATGATTCCTGCCGGAAAATCTCAAAAAGAAATGATCGAATTT
AAAGATTTAGCCGGACTGAAAAAAGTATGA

Upstream 100 bases:

>100_bases
AGAAGGTCTTTTTTTAGTAGAAGAATCGCTGATTTAAGATTACGGGTTCCTCTTGACAGTAGCCTTTTCGGTATCTTTAT
GGACGTTAAAAGGAGGAAGC

Downstream 100 bases:

>100_bases
AACACATTCTAAAAATTCTGGTAAACAACCATCCGGGAGTAATGAGCCATGTTTCCGGTTTATTTACTCGCAGAAGTTAT
AATATAGATTCGATTGCGGT

Product: thiamine pyrophosphate-requiring enzyme

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MSAESERITGARLMVELLEGYGVDVVFGYPGGAILPFYDEIYKSKKIKHILVRHEQGAVHMAEGYARATGKLGVCIATSG
PGATNLVTGLTDAKMDSVPILAITGQVATDAIGTDAFQEADIFGITIPITKYNALIKSANDIARHFEEATLIALGGRPGP
VLLDFPKDVQTGLTDVRKATRLKIAPHHYKKPEVRGNLEEFAGAINRSKKPLLYVGGGAINAVAYKEIYELATKAQIPVT
TTLMGLGSFPGTHSLSMGMLGMHGTVAANKAVLECDYILNLGARFDDRVAKIGEFAENAVRAHIDIDTAEFNKRVQVDHL
LHGDLKDTLRALIPFVQKRDRTSWIQYLQGLKKDHPLDFDNSGNAIKPQDFLERLYKKTQGKAIVSTDVGQHQMWTAQYY
LLDDPNNWLTSGGLGTMGYGLPAAIGAKFGRPDKMTICVSGDGSIQMNIQELATIAANKLGVKILVFNNNFLGMVRQWQE
LFYEERFSQSEWNFNPDFVKLAEAYSIPAMKISDKSEIDRAIEFFTKDDGAAFLEVIIPAEEKVFPMIPAGKSQKEMIEF
KDLAGLKKV

Sequences:

>Translated_569_residues
MSAESERITGARLMVELLEGYGVDVVFGYPGGAILPFYDEIYKSKKIKHILVRHEQGAVHMAEGYARATGKLGVCIATSG
PGATNLVTGLTDAKMDSVPILAITGQVATDAIGTDAFQEADIFGITIPITKYNALIKSANDIARHFEEATLIALGGRPGP
VLLDFPKDVQTGLTDVRKATRLKIAPHHYKKPEVRGNLEEFAGAINRSKKPLLYVGGGAINAVAYKEIYELATKAQIPVT
TTLMGLGSFPGTHSLSMGMLGMHGTVAANKAVLECDYILNLGARFDDRVAKIGEFAENAVRAHIDIDTAEFNKRVQVDHL
LHGDLKDTLRALIPFVQKRDRTSWIQYLQGLKKDHPLDFDNSGNAIKPQDFLERLYKKTQGKAIVSTDVGQHQMWTAQYY
LLDDPNNWLTSGGLGTMGYGLPAAIGAKFGRPDKMTICVSGDGSIQMNIQELATIAANKLGVKILVFNNNFLGMVRQWQE
LFYEERFSQSEWNFNPDFVKLAEAYSIPAMKISDKSEIDRAIEFFTKDDGAAFLEVIIPAEEKVFPMIPAGKSQKEMIEF
KDLAGLKKV
>Mature_568_residues
SAESERITGARLMVELLEGYGVDVVFGYPGGAILPFYDEIYKSKKIKHILVRHEQGAVHMAEGYARATGKLGVCIATSGP
GATNLVTGLTDAKMDSVPILAITGQVATDAIGTDAFQEADIFGITIPITKYNALIKSANDIARHFEEATLIALGGRPGPV
LLDFPKDVQTGLTDVRKATRLKIAPHHYKKPEVRGNLEEFAGAINRSKKPLLYVGGGAINAVAYKEIYELATKAQIPVTT
TLMGLGSFPGTHSLSMGMLGMHGTVAANKAVLECDYILNLGARFDDRVAKIGEFAENAVRAHIDIDTAEFNKRVQVDHLL
HGDLKDTLRALIPFVQKRDRTSWIQYLQGLKKDHPLDFDNSGNAIKPQDFLERLYKKTQGKAIVSTDVGQHQMWTAQYYL
LDDPNNWLTSGGLGTMGYGLPAAIGAKFGRPDKMTICVSGDGSIQMNIQELATIAANKLGVKILVFNNNFLGMVRQWQEL
FYEERFSQSEWNFNPDFVKLAEAYSIPAMKISDKSEIDRAIEFFTKDDGAAFLEVIIPAEEKVFPMIPAGKSQKEMIEFK
DLAGLKKV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=496, Percent_Identity=26.2096774193548, Blast_Score=143, Evalue=6e-34,
Organism=Homo sapiens, GI21361361, Length=502, Percent_Identity=25.4980079681275, Blast_Score=123, Evalue=5e-28,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=44.8028673835125, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI87081685, Length=569, Percent_Identity=42.7065026362039, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1786717, Length=576, Percent_Identity=32.2916666666667, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1787096, Length=542, Percent_Identity=27.859778597786, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1788716, Length=499, Percent_Identity=25.250501002004, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17542570, Length=513, Percent_Identity=26.1208576998051, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17531299, Length=568, Percent_Identity=25, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17531301, Length=568, Percent_Identity=25, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=577, Percent_Identity=43.6741767764298, Blast_Score=458, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320816, Length=492, Percent_Identity=23.780487804878, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19922626, Length=481, Percent_Identity=26.1954261954262, Blast_Score=145, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62480; Mature: 62348

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAESERITGARLMVELLEGYGVDVVFGYPGGAILPFYDEIYKSKKIKHILVRHEQGAVH
CCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEEECCCCCEE
MAEGYARATGKLGVCIATSGPGATNLVTGLTDAKMDSVPILAITGQVATDAIGTDAFQEA
ECCCHHHCCCCEEEEEEECCCCCHHHHHCCCCCCCCCCEEEEEECCHHHHHCCCCCCCCC
DIFGITIPITKYNALIKSANDIARHFEEATLIALGGRPGPVLLDFPKDVQTGLTDVRKAT
CEEEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEECCHHHHHHHHHHHHHH
RLKIAPHHYKKPEVRGNLEEFAGAINRSKKPLLYVGGGAINAVAYKEIYELATKAQIPVT
EEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCH
TTLMGLGSFPGTHSLSMGMLGMHGTVAANKAVLECDYILNLGARFDDRVAKIGEFAENAV
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHHE
RAHIDIDTAEFNKRVQVDHLLHGDLKDTLRALIPFVQKRDRTSWIQYLQGLKKDHPLDFD
EEEEEEEHHHCCCEEEEHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCC
NSGNAIKPQDFLERLYKKTQGKAIVSTDVGQHQMWTAQYYLLDDPNNWLTSGGLGTMGYG
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCC
LPAAIGAKFGRPDKMTICVSGDGSIQMNIQELATIAANKLGVKILVFNNNFLGMVRQWQE
CHHHHHCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHH
LFYEERFSQSEWNFNPDFVKLAEAYSIPAMKISDKSEIDRAIEFFTKDDGAAFLEVIIPA
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEEECC
EEKVFPMIPAGKSQKEMIEFKDLAGLKKV
CCCEEECCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SAESERITGARLMVELLEGYGVDVVFGYPGGAILPFYDEIYKSKKIKHILVRHEQGAVH
CCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEEECCCCCEE
MAEGYARATGKLGVCIATSGPGATNLVTGLTDAKMDSVPILAITGQVATDAIGTDAFQEA
ECCCHHHCCCCEEEEEEECCCCCHHHHHCCCCCCCCCCEEEEEECCHHHHHCCCCCCCCC
DIFGITIPITKYNALIKSANDIARHFEEATLIALGGRPGPVLLDFPKDVQTGLTDVRKAT
CEEEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEECCHHHHHHHHHHHHHH
RLKIAPHHYKKPEVRGNLEEFAGAINRSKKPLLYVGGGAINAVAYKEIYELATKAQIPVT
EEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCH
TTLMGLGSFPGTHSLSMGMLGMHGTVAANKAVLECDYILNLGARFDDRVAKIGEFAENAV
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHHE
RAHIDIDTAEFNKRVQVDHLLHGDLKDTLRALIPFVQKRDRTSWIQYLQGLKKDHPLDFD
EEEEEEEHHHCCCEEEEHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCC
NSGNAIKPQDFLERLYKKTQGKAIVSTDVGQHQMWTAQYYLLDDPNNWLTSGGLGTMGYG
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCC
LPAAIGAKFGRPDKMTICVSGDGSIQMNIQELATIAANKLGVKILVFNNNFLGMVRQWQE
CHHHHHCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHH
LFYEERFSQSEWNFNPDFVKLAEAYSIPAMKISDKSEIDRAIEFFTKDDGAAFLEVIIPA
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEEECC
EEKVFPMIPAGKSQKEMIEFKDLAGLKKV
CCCEEECCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]