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The map label for this gene is ddl [H]

Identifier: 116327785

GI number: 116327785

Start: 1219305

End: 1220366

Strand: Direct

Name: ddl [H]

Synonym: LBL_1045

Alternate gene names: 116327785

Gene position: 1219305-1220366 (Clockwise)

Preceding gene: 116327784

Following gene: 116327786

Centisome position: 33.73

GC content: 41.15

Gene sequence:

>1062_bases
ATGTATGACGTAATGCCGAGCGTTCTTGTTTACGCGGATCTTCACGAGTTTGAAGGGGAGTTTCCGGAAATTTACAAACA
GGAATGGGAATCTTCGAAATCCGTGGATGTTATCTTAGAATTGTTAAGCGAAGTCGGGGAAAGAGTCGAATTTGTGGCCA
CTCCGAACGAACTTTTGGAAAAATTAAATCATTATTCCGACTTGGATTTTACAAAACGCCCTGTTCTCTTTCATCTGATG
GAGGGATTTCGTTCCAGAAACAGGGAGTCCTTGATTCCTGCTGCGGCGGAGTTGTTTGGTTTTCCTCACACCGGTTCGGA
CGTTTATTCTCAAAACGTGTCCTTGGATAAAAATCTAACGAAAATATTCGCCCAGTCTATGGGTATTCCGATTGTGCCCG
GGTTTTTAATTCGTTTCGAGACGGATTTTACGGTTCCGAAGGAGTTTTCCTTTCCCGGATTTTTAAAACCTTCCGGAGAA
GGTTCTAGCTTCGGAATCGGAGAGGAAAGTATCATTCACGACGAAAAGGATCTCCGTTTCAAATTGTCCTCCAGAACGGC
GGAATTTTTTCCTTATCTCTTGGAAGAATATTTAACGGGAATTGAATATACTATTTCCGTGATAGGATCTAATGTAGTAG
GTTATAGAGTCAGTTCCGCGGGAAGATTGGTTCTTCGGGACGATCTGAAAGTGGAAGAGGTTTACGGAGAAAAAACGAAA
TCCAAAAGTGTGATGCCGGAAACTCTCGTATTTGATTGTCCTTCCGGTTTGGAGTTATTCCTTCAGGAACAAAGTCGGTT
GTTTTGTAGTTCCCTTGGTACTTCCGGTCCTGCGAGATTAGATTGGAAACTCGATTCTTTAGGGAATCCCTATTTTCTTG
AGATAAATCTGACTCCCGGTTTGTCTCCATTTTATTCCACATTTCCGATTTGTTATCGTCGGAGTCTAGGAAACGAAAAA
AACTTATTACAAGAGATTCTAAAAATAGCCCGTTTGGATTTTGAGACCGATCGATTTTTATATGCCAAAAAGAAAATTAG
ATCCTTATCAAGTCAAAGATAA

Upstream 100 bases:

>100_bases
CCTCCGGATTCCACAATGGAAGAAATTTACCCTGAGGTCTCCTTGGAGACCTGGGAAAGAATTTCTCCGAACGCGTTCGG
AGCCAAAGAGAAGAAATTGA

Downstream 100 bases:

>100_bases
AGTTATAGATTTATGCAGACAGTGAGTTCTATTCAAACTTCCGAATCCTTTCGAAATTCTTTGAAGGAATCGGTTCGGAA
TCATCCGGTCCTGGTTTCCA

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MYDVMPSVLVYADLHEFEGEFPEIYKQEWESSKSVDVILELLSEVGERVEFVATPNELLEKLNHYSDLDFTKRPVLFHLM
EGFRSRNRESLIPAAAELFGFPHTGSDVYSQNVSLDKNLTKIFAQSMGIPIVPGFLIRFETDFTVPKEFSFPGFLKPSGE
GSSFGIGEESIIHDEKDLRFKLSSRTAEFFPYLLEEYLTGIEYTISVIGSNVVGYRVSSAGRLVLRDDLKVEEVYGEKTK
SKSVMPETLVFDCPSGLELFLQEQSRLFCSSLGTSGPARLDWKLDSLGNPYFLEINLTPGLSPFYSTFPICYRRSLGNEK
NLLQEILKIARLDFETDRFLYAKKKIRSLSSQR

Sequences:

>Translated_353_residues
MYDVMPSVLVYADLHEFEGEFPEIYKQEWESSKSVDVILELLSEVGERVEFVATPNELLEKLNHYSDLDFTKRPVLFHLM
EGFRSRNRESLIPAAAELFGFPHTGSDVYSQNVSLDKNLTKIFAQSMGIPIVPGFLIRFETDFTVPKEFSFPGFLKPSGE
GSSFGIGEESIIHDEKDLRFKLSSRTAEFFPYLLEEYLTGIEYTISVIGSNVVGYRVSSAGRLVLRDDLKVEEVYGEKTK
SKSVMPETLVFDCPSGLELFLQEQSRLFCSSLGTSGPARLDWKLDSLGNPYFLEINLTPGLSPFYSTFPICYRRSLGNEK
NLLQEILKIARLDFETDRFLYAKKKIRSLSSQR
>Mature_353_residues
MYDVMPSVLVYADLHEFEGEFPEIYKQEWESSKSVDVILELLSEVGERVEFVATPNELLEKLNHYSDLDFTKRPVLFHLM
EGFRSRNRESLIPAAAELFGFPHTGSDVYSQNVSLDKNLTKIFAQSMGIPIVPGFLIRFETDFTVPKEFSFPGFLKPSGE
GSSFGIGEESIIHDEKDLRFKLSSRTAEFFPYLLEEYLTGIEYTISVIGSNVVGYRVSSAGRLVLRDDLKVEEVYGEKTK
SKSVMPETLVFDCPSGLELFLQEQSRLFCSSLGTSGPARLDWKLDSLGNPYFLEINLTPGLSPFYSTFPICYRRSLGNEK
NLLQEILKIARLDFETDRFLYAKKKIRSLSSQR

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=250, Percent_Identity=29.2, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786579, Length=251, Percent_Identity=23.9043824701195, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 40219; Mature: 40219

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDVMPSVLVYADLHEFEGEFPEIYKQEWESSKSVDVILELLSEVGERVEFVATPNELLE
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECHHHHHH
KLNHYSDLDFTKRPVLFHLMEGFRSRNRESLIPAAAELFGFPHTGSDVYSQNVSLDKNLT
HHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHH
KIFAQSMGIPIVPGFLIRFETDFTVPKEFSFPGFLKPSGEGSSFGIGEESIIHDEKDLRF
HHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHEE
KLSSRTAEFFPYLLEEYLTGIEYTISVIGSNVVGYRVSSAGRLVLRDDLKVEEVYGEKTK
EECCCHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCEEEEECCCCHHHHHCCHHC
SKSVMPETLVFDCPSGLELFLQEQSRLFCSSLGTSGPARLDWKLDSLGNPYFLEINLTPG
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCC
LSPFYSTFPICYRRSLGNEKNLLQEILKIARLDFETDRFLYAKKKIRSLSSQR
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYDVMPSVLVYADLHEFEGEFPEIYKQEWESSKSVDVILELLSEVGERVEFVATPNELLE
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECHHHHHH
KLNHYSDLDFTKRPVLFHLMEGFRSRNRESLIPAAAELFGFPHTGSDVYSQNVSLDKNLT
HHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHH
KIFAQSMGIPIVPGFLIRFETDFTVPKEFSFPGFLKPSGEGSSFGIGEESIIHDEKDLRF
HHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHEE
KLSSRTAEFFPYLLEEYLTGIEYTISVIGSNVVGYRVSSAGRLVLRDDLKVEEVYGEKTK
EECCCHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCEEEEECCCCHHHHHCCHHC
SKSVMPETLVFDCPSGLELFLQEQSRLFCSSLGTSGPARLDWKLDSLGNPYFLEINLTPG
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCC
LSPFYSTFPICYRRSLGNEKNLLQEILKIARLDFETDRFLYAKKKIRSLSSQR
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA