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The map label for this gene is prfC [H]

Identifier: 116327772

GI number: 116327772

Start: 1201901

End: 1203502

Strand: Direct

Name: prfC [H]

Synonym: LBL_1030

Alternate gene names: 116327772

Gene position: 1201901-1203502 (Clockwise)

Preceding gene: 116327768

Following gene: 116327774

Centisome position: 33.25

GC content: 45.57

Gene sequence:

>1602_bases
ATGAGCGAAACAGTAGAACAGAAACAATCCATCGAAGAGGAAACTCGAAGAAGGAGGACGTTTGCAATTATCGCTCACCC
GGATGCTGGAAAGACGACCCTTACGGAAAAGCTTCTACTATATGGAGGTGCGATCCAACTCGCAGGAGCCGTTAAGGCTC
GCAAGAATCGGAAGGCCGCTACCTCCGATTGGATGGAGATGGAAAAAGAAAAAGGGATTTCCATCACTTCCGCCGCACTT
CAGTTCGAATATGACGGATACGTTCTCAATCTATTGGACACACCCGGTCACGAGGATTTTTCGGAGGATACGTATCGCAC
TTTAATCGCAGCGGATACCGCGGTGATGGTGCTCGACGCCGGAAAGGGGGTCGAGCCTCAGACGATAAAGTTATTCAAAG
TTTGTAGAGATCGTGGAATTCCGATTGTAACTTTCATTAACAAGATGGATAGACCTACAAAAAATCTTTTTGTTCTTCTG
GATGAGATTGAAAAGGTTCTTGGTATATCCGCTGTGCCTATGGTGTGGCCGATCGGAACCGGTGTGGATTTCAGCGGGGT
TTATTCCCGCAAAGATAAGAAAATTCTAACATACGATAAGACTCCCGGAGGAAGTCAAAAATCCTCCTTTCAAACCTCGG
GTGTAAACGATCCGGAATTGGATTCAAGATTCGAAGACTGGGTTATCAAATCCTTTCGGGAGGAATTGGAATTAGTAGAA
GGGGGAATTTCAGAGTTCAGTCAAAAAGACTTTTTGGATTCTAAAATTACTCCCGTTTTTTTCGGTTCCGCAGTGAACAA
TTTCGGAATTCAATTGTTCTTAGATGAGTTCATCAAGATTGCTCCCCCGCCGTTATTTTTTCCGTTAAAAGACGGATCTC
GTTTGGATCCGATCCATACTCCTTTCAGCGGGTTTATCTTTAAGGTTCAGGCGAACATGAATCGTCAGCACCGAGATAGA
ATTGCGTTCTTAAGAGTGACTTCCGGTAAGTTTGAAAGAGGGCTGAACGTGCTCCATGTAAGATTGGGGAAATCCGTAAA
ACTTTCCTCTTCTTTCGCTTTTTTCGGACAGGATCGAAATACGGTCGATGAGGCGTATCCGGGGGACATCATTGGCCTCG
TAAATCCGGGCACGTATGCGATCGGAGATATAGTCGCTTCTTCTAAGGTTCCCGATTTAAAATCTCTTCCCGTATTCGCA
CCCGAACTTTTTGCAACGATCTCATCCGCCGATACGGCAAGTATGAAAAGCTTTCGAAAAGGAATCGAACAACTTGCCGA
AGAGGGAATTCTTCATTTGTTCTCTTCTCAGACGATCGGTGGAGGTCTGCCCATTATCGGCGCTATGGGACAGCTTCAAT
TCGAAGTATTTCGAAGAAGACTCTTGGACGAGTACAACGCGCCTTCCACGATCACGATTCTTCCTTATATAATCTCTTGT
TGGATCGGGCGGGAAGATCTGGCAAAGGTTCCGTCTTCCGCAAATTTAGTCACTGATCGGGGAGGGAGGGCCGCTCTTCT
CTTCGATACGGAATGGGACAAGGGATATTTTCAGAAGAAAAATCCCGAAATAACTCTTTTAGATTATCCTCCGACTGCAT
AA

Upstream 100 bases:

>100_bases
AAGTACGAAAGATGTGGGAACTCCCACAAAGATCCGTTCGTTTTAATTGCTGACAGTCGACTTTGTAAAGAAAATCTGGA
AAAGAGTGCGGGGCAAAGTA

Downstream 100 bases:

>100_bases
CGAAATTTTCAGACGGTGAAAAAATCTCTCGGGGTAGTAAGAATTCGATTCTTTTCGAAGTCTTAGATATTGTATTTGCA
CCAGATGTCTTTGAGGCGAA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MSETVEQKQSIEEETRRRRTFAIIAHPDAGKTTLTEKLLLYGGAIQLAGAVKARKNRKAATSDWMEMEKEKGISITSAAL
QFEYDGYVLNLLDTPGHEDFSEDTYRTLIAADTAVMVLDAGKGVEPQTIKLFKVCRDRGIPIVTFINKMDRPTKNLFVLL
DEIEKVLGISAVPMVWPIGTGVDFSGVYSRKDKKILTYDKTPGGSQKSSFQTSGVNDPELDSRFEDWVIKSFREELELVE
GGISEFSQKDFLDSKITPVFFGSAVNNFGIQLFLDEFIKIAPPPLFFPLKDGSRLDPIHTPFSGFIFKVQANMNRQHRDR
IAFLRVTSGKFERGLNVLHVRLGKSVKLSSSFAFFGQDRNTVDEAYPGDIIGLVNPGTYAIGDIVASSKVPDLKSLPVFA
PELFATISSADTASMKSFRKGIEQLAEEGILHLFSSQTIGGGLPIIGAMGQLQFEVFRRRLLDEYNAPSTITILPYIISC
WIGREDLAKVPSSANLVTDRGGRAALLFDTEWDKGYFQKKNPEITLLDYPPTA

Sequences:

>Translated_533_residues
MSETVEQKQSIEEETRRRRTFAIIAHPDAGKTTLTEKLLLYGGAIQLAGAVKARKNRKAATSDWMEMEKEKGISITSAAL
QFEYDGYVLNLLDTPGHEDFSEDTYRTLIAADTAVMVLDAGKGVEPQTIKLFKVCRDRGIPIVTFINKMDRPTKNLFVLL
DEIEKVLGISAVPMVWPIGTGVDFSGVYSRKDKKILTYDKTPGGSQKSSFQTSGVNDPELDSRFEDWVIKSFREELELVE
GGISEFSQKDFLDSKITPVFFGSAVNNFGIQLFLDEFIKIAPPPLFFPLKDGSRLDPIHTPFSGFIFKVQANMNRQHRDR
IAFLRVTSGKFERGLNVLHVRLGKSVKLSSSFAFFGQDRNTVDEAYPGDIIGLVNPGTYAIGDIVASSKVPDLKSLPVFA
PELFATISSADTASMKSFRKGIEQLAEEGILHLFSSQTIGGGLPIIGAMGQLQFEVFRRRLLDEYNAPSTITILPYIISC
WIGREDLAKVPSSANLVTDRGGRAALLFDTEWDKGYFQKKNPEITLLDYPPTA
>Mature_532_residues
SETVEQKQSIEEETRRRRTFAIIAHPDAGKTTLTEKLLLYGGAIQLAGAVKARKNRKAATSDWMEMEKEKGISITSAALQ
FEYDGYVLNLLDTPGHEDFSEDTYRTLIAADTAVMVLDAGKGVEPQTIKLFKVCRDRGIPIVTFINKMDRPTKNLFVLLD
EIEKVLGISAVPMVWPIGTGVDFSGVYSRKDKKILTYDKTPGGSQKSSFQTSGVNDPELDSRFEDWVIKSFREELELVEG
GISEFSQKDFLDSKITPVFFGSAVNNFGIQLFLDEFIKIAPPPLFFPLKDGSRLDPIHTPFSGFIFKVQANMNRQHRDRI
AFLRVTSGKFERGLNVLHVRLGKSVKLSSSFAFFGQDRNTVDEAYPGDIIGLVNPGTYAIGDIVASSKVPDLKSLPVFAP
ELFATISSADTASMKSFRKGIEQLAEEGILHLFSSQTIGGGLPIIGAMGQLQFEVFRRRLLDEYNAPSTITILPYIISCW
IGREDLAKVPSSANLVTDRGGRAALLFDTEWDKGYFQKKNPEITLLDYPPTA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=478, Percent_Identity=29.2887029288703, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI19923640, Length=492, Percent_Identity=27.0325203252033, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI25306283, Length=387, Percent_Identity=29.4573643410853, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI25306287, Length=286, Percent_Identity=33.5664335664336, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI157426893, Length=172, Percent_Identity=34.3023255813954, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=34.0136054421769, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI4503483, Length=164, Percent_Identity=32.9268292682927, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI310132016, Length=131, Percent_Identity=32.824427480916, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI310110807, Length=131, Percent_Identity=32.824427480916, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI310123363, Length=131, Percent_Identity=32.824427480916, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790835, Length=518, Percent_Identity=44.4015444015444, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1789738, Length=487, Percent_Identity=28.7474332648871, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1788922, Length=178, Percent_Identity=37.6404494382022, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI48994988, Length=168, Percent_Identity=35.1190476190476, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=483, Percent_Identity=25.879917184265, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=477, Percent_Identity=26.8343815513627, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17557151, Length=163, Percent_Identity=36.1963190184049, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=171, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=30.3703703703704, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=30.3703703703704, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=477, Percent_Identity=29.3501048218029, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=513, Percent_Identity=28.2651072124756, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6323320, Length=167, Percent_Identity=30.5389221556886, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6324707, Length=171, Percent_Identity=33.9181286549708, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320593, Length=171, Percent_Identity=33.9181286549708, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24582462, Length=480, Percent_Identity=29.1666666666667, Blast_Score=188, Evalue=1e-47,
Organism=Drosophila melanogaster, GI221458488, Length=497, Percent_Identity=24.9496981891348, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI78706572, Length=173, Percent_Identity=35.2601156069364, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585709, Length=168, Percent_Identity=31.547619047619, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24585711, Length=159, Percent_Identity=32.0754716981132, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24585713, Length=159, Percent_Identity=32.0754716981132, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI28574573, Length=178, Percent_Identity=33.1460674157303, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24656849, Length=146, Percent_Identity=32.8767123287671, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59214; Mature: 59083

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETVEQKQSIEEETRRRRTFAIIAHPDAGKTTLTEKLLLYGGAIQLAGAVKARKNRKAA
CCCHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCH
TSDWMEMEKEKGISITSAALQFEYDGYVLNLLDTPGHEDFSEDTYRTLIAADTAVMVLDA
HHHHHHHHHHCCCEEEEEEEEEEECCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEC
GKGVEPQTIKLFKVCRDRGIPIVTFINKMDRPTKNLFVLLDEIEKVLGISAVPMVWPIGT
CCCCCCHHHHHHHHHHHCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCC
GVDFSGVYSRKDKKILTYDKTPGGSQKSSFQTSGVNDPELDSRFEDWVIKSFREELELVE
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GGISEFSQKDFLDSKITPVFFGSAVNNFGIQLFLDEFIKIAPPPLFFPLKDGSRLDPIHT
CCHHHHHHHHHCCCCCCCEEECCHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PFSGFIFKVQANMNRQHRDRIAFLRVTSGKFERGLNVLHVRLGKSVKLSSSFAFFGQDRN
CCCCEEEEEEECCCHHHCCEEEEEEEECCCHHHCCEEEEEECCCCEEECCCEEEECCCCC
TVDEAYPGDIIGLVNPGTYAIGDIVASSKVPDLKSLPVFAPELFATISSADTASMKSFRK
CHHHCCCCCEEEEECCCCEEHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
GIEQLAEEGILHLFSSQTIGGGLPIIGAMGQLQFEVFRRRLLDEYNAPSTITILPYIISC
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHH
WIGREDLAKVPSSANLVTDRGGRAALLFDTEWDKGYFQKKNPEITLLDYPPTA
HCCCHHHHHCCCCCCEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SETVEQKQSIEEETRRRRTFAIIAHPDAGKTTLTEKLLLYGGAIQLAGAVKARKNRKAA
CCHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCH
TSDWMEMEKEKGISITSAALQFEYDGYVLNLLDTPGHEDFSEDTYRTLIAADTAVMVLDA
HHHHHHHHHHCCCEEEEEEEEEEECCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEC
GKGVEPQTIKLFKVCRDRGIPIVTFINKMDRPTKNLFVLLDEIEKVLGISAVPMVWPIGT
CCCCCCHHHHHHHHHHHCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCC
GVDFSGVYSRKDKKILTYDKTPGGSQKSSFQTSGVNDPELDSRFEDWVIKSFREELELVE
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GGISEFSQKDFLDSKITPVFFGSAVNNFGIQLFLDEFIKIAPPPLFFPLKDGSRLDPIHT
CCHHHHHHHHHCCCCCCCEEECCHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PFSGFIFKVQANMNRQHRDRIAFLRVTSGKFERGLNVLHVRLGKSVKLSSSFAFFGQDRN
CCCCEEEEEEECCCHHHCCEEEEEEEECCCHHHCCEEEEEECCCCEEECCCEEEECCCCC
TVDEAYPGDIIGLVNPGTYAIGDIVASSKVPDLKSLPVFAPELFATISSADTASMKSFRK
CHHHCCCCCEEEEECCCCEEHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
GIEQLAEEGILHLFSSQTIGGGLPIIGAMGQLQFEVFRRRLLDEYNAPSTITILPYIISC
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHH
WIGREDLAKVPSSANLVTDRGGRAALLFDTEWDKGYFQKKNPEITLLDYPPTA
HCCCHHHHHCCCCCCEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA