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The map label for this gene is rsmI

Identifier: 116327748

GI number: 116327748

Start: 1173155

End: 1173916

Strand: Direct

Name: rsmI

Synonym: LBL_1004

Alternate gene names: 116327748

Gene position: 1173155-1173916 (Clockwise)

Preceding gene: 116327747

Following gene: 116327751

Centisome position: 32.46

GC content: 43.7

Gene sequence:

>762_bases
ATGAAAAAAGAATTACAAGGGGCTCTCGTTCTGGTTTCGGTTTCTTTGGGAAATTTCGGCGACTTGACCGCAAGGGCTAG
ACATTTATTAGAATATTGTGATATACTCATAGGTGAGGAATTCAGGACGACTTCCACTCTTCTTAAATCCCTTTCGATCT
CCAAACAATTTCTTCTCTGCAACGAACATACAACCCCCGAAGAGATTCGCAGTCTCGGTCAAATTGTGGTGGATTCCGGT
TTGACCGTTTTGGTTTCCGATGCGGGAACTCCCGGAATCGAAGATCCGGGAAGAGAACTCGTCGGGGAGGTTTTAAGAAG
AGGGGGACGCGTACAAAGCGCTCCCGGTCCGATTGCTTTCGGAGCGGCTTTAAGCATTTCCGGTTTTAAGACTTCTCCGT
TTACTTTTTGCGGTTTTTTATCCAGGGATTCTTCGGAACGAAAGTTGGAATTAAGTCGCTATTTAAAACCGGGACATACG
GTCGTATTTTATGAAACACCTTACAGATATAAGGCCGTTCTTCGCGATTTAGATTCCGTTTTGATAGAAACGGGAGAGGA
TAGGGCGATCTTTTTCTGTTTGGACCTGACCTTGGATTCTGAATTTCAGTTTCGAGGAAAGTTAGGCGAACTTTTGAAGG
TTTTGGATACGCTTCCAAAAGGAAATCCTGTGATTGTCGTTTCTCAGAGAAAGGAACAACGGAGTCAAAGGTCTTTTTCA
AAGGGCGATAAGAAACCTTCTTTTAAACGCTTCAAGAAATGA

Upstream 100 bases:

>100_bases
ATTCCTAATGAAAGCCTTCTTGTATGAATCAATTTGATGCGCGCGAATATTCGGAACGTATTATGAAGGAAAATTTTAGG
TTTGAGAGAATCCGTAAAGT

Downstream 100 bases:

>100_bases
ATGATTGCAGCCTGATCGAATTCTAATATATATTTATAATATTATAAATTATAAATATATCTTTAATTTTAAGATGTCCT
CGGACGTTACTCATCTCTAC

Product: methyltransferase

Products: NA

Alternate protein names: 16S rRNA 2'-O-ribose C1402 methyltransferase; rRNA (cytidine-2'-O-)-methyltransferase RsmI

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MKKELQGALVLVSVSLGNFGDLTARARHLLEYCDILIGEEFRTTSTLLKSLSISKQFLLCNEHTTPEEIRSLGQIVVDSG
LTVLVSDAGTPGIEDPGRELVGEVLRRGGRVQSAPGPIAFGAALSISGFKTSPFTFCGFLSRDSSERKLELSRYLKPGHT
VVFYETPYRYKAVLRDLDSVLIETGEDRAIFFCLDLTLDSEFQFRGKLGELLKVLDTLPKGNPVIVVSQRKEQRSQRSFS
KGDKKPSFKRFKK

Sequences:

>Translated_253_residues
MKKELQGALVLVSVSLGNFGDLTARARHLLEYCDILIGEEFRTTSTLLKSLSISKQFLLCNEHTTPEEIRSLGQIVVDSG
LTVLVSDAGTPGIEDPGRELVGEVLRRGGRVQSAPGPIAFGAALSISGFKTSPFTFCGFLSRDSSERKLELSRYLKPGHT
VVFYETPYRYKAVLRDLDSVLIETGEDRAIFFCLDLTLDSEFQFRGKLGELLKVLDTLPKGNPVIVVSQRKEQRSQRSFS
KGDKKPSFKRFKK
>Mature_253_residues
MKKELQGALVLVSVSLGNFGDLTARARHLLEYCDILIGEEFRTTSTLLKSLSISKQFLLCNEHTTPEEIRSLGQIVVDSG
LTVLVSDAGTPGIEDPGRELVGEVLRRGGRVQSAPGPIAFGAALSISGFKTSPFTFCGFLSRDSSERKLELSRYLKPGHT
VVFYETPYRYKAVLRDLDSVLIETGEDRAIFFCLDLTLDSEFQFRGKLGELLKVLDTLPKGNPVIVVSQRKEQRSQRSFS
KGDKKPSFKRFKK

Specific function: Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA

COG id: COG0313

COG function: function code R; Predicted methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmI family

Homologues:

Organism=Escherichia coli, GI1789535, Length=231, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMI_LEPBL (Q053G0)

Other databases:

- EMBL:   CP000348
- RefSeq:   YP_797468.1
- ProteinModelPortal:   Q053G0
- SMR:   Q053G0
- STRING:   Q053G0
- GeneID:   4408686
- GenomeReviews:   CP000348_GR
- KEGG:   lbl:LBL_1004
- eggNOG:   COG0313
- HOGENOM:   HBG529058
- OMA:   KELIYYL
- ProtClustDB:   CLSK574637
- BioCyc:   LBOR355276:LBL_1004-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01877
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR008189
- Gene3D:   G3DSA:3.40.1010.10
- TIGRFAMs:   TIGR00096

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: NA

Molecular weight: Translated: 28124; Mature: 28124

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS01296 RSMI

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKELQGALVLVSVSLGNFGDLTARARHLLEYCDILIGEEFRTTSTLLKSLSISKQFLLC
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEE
NEHTTPEEIRSLGQIVVDSGLTVLVSDAGTPGIEDPGRELVGEVLRRGGRVQSAPGPIAF
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEE
GAALSISGFKTSPFTFCGFLSRDSSERKLELSRYLKPGHTVVFYETPYRYKAVLRDLDSV
CCEEEECCCCCCCHHEEHHHCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHH
LIETGEDRAIFFCLDLTLDSEFQFRGKLGELLKVLDTLPKGNPVIVVSQRKEQRSQRSFS
HHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHH
KGDKKPSFKRFKK
CCCCCCCHHHHCC
>Mature Secondary Structure
MKKELQGALVLVSVSLGNFGDLTARARHLLEYCDILIGEEFRTTSTLLKSLSISKQFLLC
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEE
NEHTTPEEIRSLGQIVVDSGLTVLVSDAGTPGIEDPGRELVGEVLRRGGRVQSAPGPIAF
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEE
GAALSISGFKTSPFTFCGFLSRDSSERKLELSRYLKPGHTVVFYETPYRYKAVLRDLDSV
CCEEEECCCCCCCHHEEHHHCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHH
LIETGEDRAIFFCLDLTLDSEFQFRGKLGELLKVLDTLPKGNPVIVVSQRKEQRSQRSFS
HHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHH
KGDKKPSFKRFKK
CCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA