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The map label for this gene is pykF [H]

Identifier: 116327588

GI number: 116327588

Start: 947018

End: 948445

Strand: Reverse

Name: pykF [H]

Synonym: LBL_0819

Alternate gene names: 116327588

Gene position: 948445-947018 (Counterclockwise)

Preceding gene: 116327591

Following gene: 116327587

Centisome position: 26.24

GC content: 43.56

Gene sequence:

>1428_bases
ATGAAAATCCTGAACTGGAAAAAAACCAAAATCGTTTGTACCATCGGTCCTGCCTCTTCTTCCGAAGAGACCATCCTTTC
CATGCTCAAAGCGGGAATGGATATCGCTCGAATGAATTTCTCGCACGGAACTCACGACTCTCATAAAAGAGTTTACGATA
CGTTACGCAAATGCGAACAAACCTTCGGTTTTCCATTGGGAATCATGGCCGATTTGCAAGGTCCAAAAATTCGAACCGGA
AAATTAAAACTCAATTCCATTCTACTCCATAAAAATCAAGAGATTCAAATCGTTCCCGACGCGGATTTCCAGGGTGACGA
ACATACGATCGGCTGCACCTATCCGAATCTAATTCAAGATATTAAAGAAGGAGATAAGATCCTCATCGACGACGGTAAAC
TAATTCTGAAAGTCATTTTTAAAAAGTCGGACTCCGCAATTTTAAAAGTGATCGTAGGCGGCATCTTATGGAGTAATAAA
GGAATTAATCTTCCGGGAACTCCAATCTCCGCTCCCGCTCTTTCCGAAAAGGACGTAGAAGATCTGAAATTTGCGCTTTC
TCTCGGGGTAGATTACATTGCACTTAGTTTCGTTCGGACGAGTGCGGATTTGGAATTAGCCAGATCTTTATTGCAAGGAA
CCTATACCGGATTGATCGCAAAAATCGAAAGACCGGAAGCGATCGAAAACATAGAAGAAATCATAGAACGCGCAGATGGA
ATCATGATTGCAAGAGGGGATCTTGGGGTCGAGATCGAAACGGAAAAAGTCCCGATCCTTCAAAAGGAACTCATCTACAA
ACTCAATCAAGCGGGCAAACCAGTGATCACGGCGACACAGATGTTGGAATCAATGATCGAAAATCCGAGACCGACCCGTG
CCGAAGCAAGCGACGTCGCAAACGCAGTTATGGATGGAACGGACGCAGTGATGCTCTCTGCGGAATCCGCAAGCGGCCAT
TATCCTTTGGAATCGGTGGAGATCATGTCGAAGATCATTCAAGAAACGGAAACCATCAACCATATCTATGAAATCCACTG
GAATATTAAGAAGACATTCTTAGAATCCGAAAGAACCGCCTTAGGAAATGCCGCGAGAGAAATCGCTCACGGAATTCACG
CAAAAGCGATCGTTAATTTCACTAGAAGCGGCTATTCCGCTTTGATCACTTCGGAAATGAGACCGAAGGTTCCGATCTAT
TCGTTTACTCCCTTTGCCACTACAGCAAGAAAAATGAAACTCTACCGAGGTGTGATACCTTTCGTAATGCCTTTTTTCAC
AAGATTGGAAGATATGATCGCATACATGAATCAGAAACTCAAAGAGGACGAATTTCTCTTTCCGGGAGACAAGATTGTGA
TTCTTTCCGGCGCTCCAGGAACTACAATACGTAGCGTCGATTTTTTACAGATTTATCAAATCCATTGA

Upstream 100 bases:

>100_bases
GAGGACGCCTATTTTTGGTTCATTCTATTTTTCGGTTTCCGTTCGTAAAGAGGAAATATTAGCTGGAACAATGGAAGGCA
TTTATCTATAATTACCGTTC

Downstream 100 bases:

>100_bases
GCTTCTTCCTTCTACAAACCCCTAAAGATAAAAATTTTTCCATTCAATCTTTCTTGGATGAAATTTTTAAAAAATTCTCC
AATCCGACAATCAGAGCCGG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 1 [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKILNWKKTKIVCTIGPASSSEETILSMLKAGMDIARMNFSHGTHDSHKRVYDTLRKCEQTFGFPLGIMADLQGPKIRTG
KLKLNSILLHKNQEIQIVPDADFQGDEHTIGCTYPNLIQDIKEGDKILIDDGKLILKVIFKKSDSAILKVIVGGILWSNK
GINLPGTPISAPALSEKDVEDLKFALSLGVDYIALSFVRTSADLELARSLLQGTYTGLIAKIERPEAIENIEEIIERADG
IMIARGDLGVEIETEKVPILQKELIYKLNQAGKPVITATQMLESMIENPRPTRAEASDVANAVMDGTDAVMLSAESASGH
YPLESVEIMSKIIQETETINHIYEIHWNIKKTFLESERTALGNAAREIAHGIHAKAIVNFTRSGYSALITSEMRPKVPIY
SFTPFATTARKMKLYRGVIPFVMPFFTRLEDMIAYMNQKLKEDEFLFPGDKIVILSGAPGTTIRSVDFLQIYQIH

Sequences:

>Translated_475_residues
MKILNWKKTKIVCTIGPASSSEETILSMLKAGMDIARMNFSHGTHDSHKRVYDTLRKCEQTFGFPLGIMADLQGPKIRTG
KLKLNSILLHKNQEIQIVPDADFQGDEHTIGCTYPNLIQDIKEGDKILIDDGKLILKVIFKKSDSAILKVIVGGILWSNK
GINLPGTPISAPALSEKDVEDLKFALSLGVDYIALSFVRTSADLELARSLLQGTYTGLIAKIERPEAIENIEEIIERADG
IMIARGDLGVEIETEKVPILQKELIYKLNQAGKPVITATQMLESMIENPRPTRAEASDVANAVMDGTDAVMLSAESASGH
YPLESVEIMSKIIQETETINHIYEIHWNIKKTFLESERTALGNAAREIAHGIHAKAIVNFTRSGYSALITSEMRPKVPIY
SFTPFATTARKMKLYRGVIPFVMPFFTRLEDMIAYMNQKLKEDEFLFPGDKIVILSGAPGTTIRSVDFLQIYQIH
>Mature_475_residues
MKILNWKKTKIVCTIGPASSSEETILSMLKAGMDIARMNFSHGTHDSHKRVYDTLRKCEQTFGFPLGIMADLQGPKIRTG
KLKLNSILLHKNQEIQIVPDADFQGDEHTIGCTYPNLIQDIKEGDKILIDDGKLILKVIFKKSDSAILKVIVGGILWSNK
GINLPGTPISAPALSEKDVEDLKFALSLGVDYIALSFVRTSADLELARSLLQGTYTGLIAKIERPEAIENIEEIIERADG
IMIARGDLGVEIETEKVPILQKELIYKLNQAGKPVITATQMLESMIENPRPTRAEASDVANAVMDGTDAVMLSAESASGH
YPLESVEIMSKIIQETETINHIYEIHWNIKKTFLESERTALGNAAREIAHGIHAKAIVNFTRSGYSALITSEMRPKVPIY
SFTPFATTARKMKLYRGVIPFVMPFFTRLEDMIAYMNQKLKEDEFLFPGDKIVILSGAPGTTIRSVDFLQIYQIH

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=479, Percent_Identity=40.9185803757829, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI33286422, Length=479, Percent_Identity=40.5010438413361, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI33286420, Length=479, Percent_Identity=40.5010438413361, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI10835121, Length=479, Percent_Identity=40.0835073068894, Blast_Score=328, Evalue=6e-90,
Organism=Homo sapiens, GI32967597, Length=479, Percent_Identity=40.0835073068894, Blast_Score=328, Evalue=8e-90,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=41.3793103448276, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=41.3793103448276, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=38.2978723404255, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=38.2978723404255, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=36.4485981308411, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1787965, Length=465, Percent_Identity=42.1505376344086, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=38.8773388773389, Blast_Score=318, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI17544584, Length=467, Percent_Identity=40.8993576017131, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17506829, Length=470, Percent_Identity=40.2127659574468, Blast_Score=305, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI71984413, Length=470, Percent_Identity=40.2127659574468, Blast_Score=305, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI71984406, Length=470, Percent_Identity=40.2127659574468, Blast_Score=305, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI17506831, Length=470, Percent_Identity=40.2127659574468, Blast_Score=305, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6319279, Length=470, Percent_Identity=40.6382978723404, Blast_Score=308, Evalue=8e-85,
Organism=Saccharomyces cerevisiae, GI6324923, Length=447, Percent_Identity=41.6107382550336, Blast_Score=296, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24648964, Length=471, Percent_Identity=42.6751592356688, Blast_Score=338, Evalue=5e-93,
Organism=Drosophila melanogaster, GI28571814, Length=471, Percent_Identity=42.6751592356688, Blast_Score=337, Evalue=8e-93,
Organism=Drosophila melanogaster, GI24648966, Length=409, Percent_Identity=41.320293398533, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24581235, Length=466, Percent_Identity=33.9055793991416, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24646914, Length=269, Percent_Identity=40.1486988847584, Blast_Score=189, Evalue=3e-48,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52835; Mature: 52835

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILNWKKTKIVCTIGPASSSEETILSMLKAGMDIARMNFSHGTHDSHKRVYDTLRKCEQ
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
TFGFPLGIMADLQGPKIRTGKLKLNSILLHKNQEIQIVPDADFQGDEHTIGCTYPNLIQD
HHCCCEEEEECCCCCCEEECCEEEEEEEEECCCEEEEEECCCCCCCCCEEECCCHHHHHH
IKEGDKILIDDGKLILKVIFKKSDSAILKVIVGGILWSNKGINLPGTPISAPALSEKDVE
HHCCCEEEEECCCEEHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
DLKFALSLGVDYIALSFVRTSADLELARSLLQGTYTGLIAKIERPEAIENIEEIIERADG
HHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCC
IMIARGDLGVEIETEKVPILQKELIYKLNQAGKPVITATQMLESMIENPRPTRAEASDVA
EEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
NAVMDGTDAVMLSAESASGHYPLESVEIMSKIIQETETINHIYEIHWNIKKTFLESERTA
HHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHH
LGNAAREIAHGIHAKAIVNFTRSGYSALITSEMRPKVPIYSFTPFATTARKMKLYRGVIP
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHH
FVMPFFTRLEDMIAYMNQKLKEDEFLFPGDKIVILSGAPGTTIRSVDFLQIYQIH
HHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEECCCCCCEECCCEEEEEEEC
>Mature Secondary Structure
MKILNWKKTKIVCTIGPASSSEETILSMLKAGMDIARMNFSHGTHDSHKRVYDTLRKCEQ
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
TFGFPLGIMADLQGPKIRTGKLKLNSILLHKNQEIQIVPDADFQGDEHTIGCTYPNLIQD
HHCCCEEEEECCCCCCEEECCEEEEEEEEECCCEEEEEECCCCCCCCCEEECCCHHHHHH
IKEGDKILIDDGKLILKVIFKKSDSAILKVIVGGILWSNKGINLPGTPISAPALSEKDVE
HHCCCEEEEECCCEEHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
DLKFALSLGVDYIALSFVRTSADLELARSLLQGTYTGLIAKIERPEAIENIEEIIERADG
HHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCC
IMIARGDLGVEIETEKVPILQKELIYKLNQAGKPVITATQMLESMIENPRPTRAEASDVA
EEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
NAVMDGTDAVMLSAESASGHYPLESVEIMSKIIQETETINHIYEIHWNIKKTFLESERTA
HHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHH
LGNAAREIAHGIHAKAIVNFTRSGYSALITSEMRPKVPIYSFTPFATTARKMKLYRGVIP
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHH
FVMPFFTRLEDMIAYMNQKLKEDEFLFPGDKIVILSGAPGTTIRSVDFLQIYQIH
HHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEECCCCCCEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]