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The map label for this gene is yegE [H]

Identifier: 116327587

GI number: 116327587

Start: 945272

End: 946243

Strand: Reverse

Name: yegE [H]

Synonym: LBL_0818

Alternate gene names: 116327587

Gene position: 946243-945272 (Counterclockwise)

Preceding gene: 116327588

Following gene: 116327581

Centisome position: 26.18

GC content: 40.02

Gene sequence:

>972_bases
ATGAACTTTAAAGAAGAATACAATCTTGAAAAACTCGTGAATAATTCTATAGATCTACTGTCAATAGTAGACTTAAAAGG
CAAAGTTCTATTGGTAAATCCTGCTTTCGAACGGACTCTAGGCTGGAAAGAAGAGGAATTGGTCGGAAGAAACCCATTTC
ACTTGCTTCACCCTGAAGATAGGGAAATCACTTTTCAAGAGTTCGAAAAATTGAACCAAGGTTTACCGACTTTTTCTTTC
CAAAATCGTTACATTTGTAGGGATGGAAATTATAAATATTTTACCTGGACAGCCTCTCCTGATCTTAATTCCGGTTTAAT
TTATGTGACCGGAAAGGATATCAGCGAAATGATCGAATCCAACCGTAAGATCAGTCAACTTGCAGCCGAACTCAAAGTCG
CGAATGATAAATTGTTTGAACAGGCTTGTACAGATCCGTTGACAAAGCTCAAGAACAGAAGAGCCTTCAACGAAGATTTG
AATCGCCTGATTCATCTCGCTCAAAATCAGGGAACTTCACTTTCCTTGTTGATGATCGACGTCGACTTTTTCAAAGACTA
TAACGACAATTTTGGTCATCCGGCGGGAGACAAAGTTCTGATTGAAATCGCCCTCTTACTCACCAGAGCCTTGCGCAAAT
GCGACGTTCTGGCAAGATATGGCGGTGAAGAATTCATTATCGCTTTACCGGATGCTTCACAAACGGATTCCGTAGAAATC
ACAGAAAGATTAATTCAAACCATCAGAGAATTTTCTTGGGAAAAAAGACCCATTACCATCAGTATCGGCATTTCAACCCT
TACTTTTCCTCTCGGTGGAACTCTTCCAATCGAAAATAATGCCTGTCTGACGAATCTAATCGAATCCGCAGATCGTGCGT
TATACGCATCAAAAACGGAAGGACGTGACAGGTTTACCCATTTTACCGAAATTCATTCTCCTGATAGATTGAATCTTTCC
TCTTTTCAGTAA

Upstream 100 bases:

>100_bases
TTTGTATAATGAAAAACTTTACGGGTATGTTTTAAAAATGAAAACCGATCTCTCACATCATATCTCCATAAAAAACTAAA
ATAATGGAAATAGACAAACG

Downstream 100 bases:

>100_bases
GAAGAACCGATCTCGCAAAATACCACGACAAGTTTTCATTTTTAAAACAAGAATTACTCTTATCTTTGATAGAAGTACAA
CAGGATTTTAATCTTTACTT

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNFKEEYNLEKLVNNSIDLLSIVDLKGKVLLVNPAFERTLGWKEEELVGRNPFHLLHPEDREITFQEFEKLNQGLPTFSF
QNRYICRDGNYKYFTWTASPDLNSGLIYVTGKDISEMIESNRKISQLAAELKVANDKLFEQACTDPLTKLKNRRAFNEDL
NRLIHLAQNQGTSLSLLMIDVDFFKDYNDNFGHPAGDKVLIEIALLLTRALRKCDVLARYGGEEFIIALPDASQTDSVEI
TERLIQTIREFSWEKRPITISIGISTLTFPLGGTLPIENNACLTNLIESADRALYASKTEGRDRFTHFTEIHSPDRLNLS
SFQ

Sequences:

>Translated_323_residues
MNFKEEYNLEKLVNNSIDLLSIVDLKGKVLLVNPAFERTLGWKEEELVGRNPFHLLHPEDREITFQEFEKLNQGLPTFSF
QNRYICRDGNYKYFTWTASPDLNSGLIYVTGKDISEMIESNRKISQLAAELKVANDKLFEQACTDPLTKLKNRRAFNEDL
NRLIHLAQNQGTSLSLLMIDVDFFKDYNDNFGHPAGDKVLIEIALLLTRALRKCDVLARYGGEEFIIALPDASQTDSVEI
TERLIQTIREFSWEKRPITISIGISTLTFPLGGTLPIENNACLTNLIESADRALYASKTEGRDRFTHFTEIHSPDRLNLS
SFQ
>Mature_323_residues
MNFKEEYNLEKLVNNSIDLLSIVDLKGKVLLVNPAFERTLGWKEEELVGRNPFHLLHPEDREITFQEFEKLNQGLPTFSF
QNRYICRDGNYKYFTWTASPDLNSGLIYVTGKDISEMIESNRKISQLAAELKVANDKLFEQACTDPLTKLKNRRAFNEDL
NRLIHLAQNQGTSLSLLMIDVDFFKDYNDNFGHPAGDKVLIEIALLLTRALRKCDVLARYGGEEFIIALPDASQTDSVEI
TERLIQTIREFSWEKRPITISIGISTLTFPLGGTLPIENNACLTNLIESADRALYASKTEGRDRFTHFTEIHSPDRLNLS
SFQ

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=320, Percent_Identity=30.625, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87082007, Length=184, Percent_Identity=36.4130434782609, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693134, Length=187, Percent_Identity=35.2941176470588, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1787541, Length=310, Percent_Identity=26.4516129032258, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081881, Length=178, Percent_Identity=34.8314606741573, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=31.25, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787802, Length=164, Percent_Identity=32.3170731707317, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081977, Length=123, Percent_Identity=38.2113821138211, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788956, Length=266, Percent_Identity=28.1954887218045, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=33.75, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786584, Length=182, Percent_Identity=27.4725274725275, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081974, Length=161, Percent_Identity=27.3291925465839, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787056, Length=173, Percent_Identity=30.635838150289, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013655 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF05231 MASE1; PF08447 PAS_3; PF08448 PAS_4 [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 36943; Mature: 36943

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKEEYNLEKLVNNSIDLLSIVDLKGKVLLVNPAFERTLGWKEEELVGRNPFHLLHPED
CCCCHHCCHHHHHCCCCCEEEEEECCCCEEEECCHHHHHCCCCHHHHCCCCCEEEECCCC
REITFQEFEKLNQGLPTFSFQNRYICRDGNYKYFTWTASPDLNSGLIYVTGKDISEMIES
CCCCHHHHHHHHCCCCCEECCCEEEEECCCEEEEEEECCCCCCCCEEEEECCCHHHHHHC
NRKISQLAAELKVANDKLFEQACTDPLTKLKNRRAFNEDLNRLIHLAQNQGTSLSLLMID
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEEEE
VDFFKDYNDNFGHPAGDKVLIEIALLLTRALRKCDVLARYGGEEFIIALPDASQTDSVEI
EHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
TERLIQTIREFSWEKRPITISIGISTLTFPLGGTLPIENNACLTNLIESADRALYASKTE
HHHHHHHHHHCCCCCCCEEEEEECEEEEECCCCEEECCCCHHHHHHHHHHHHHEEECCCC
GRDRFTHFTEIHSPDRLNLSSFQ
CCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNFKEEYNLEKLVNNSIDLLSIVDLKGKVLLVNPAFERTLGWKEEELVGRNPFHLLHPED
CCCCHHCCHHHHHCCCCCEEEEEECCCCEEEECCHHHHHCCCCHHHHCCCCCEEEECCCC
REITFQEFEKLNQGLPTFSFQNRYICRDGNYKYFTWTASPDLNSGLIYVTGKDISEMIES
CCCCHHHHHHHHCCCCCEECCCEEEEECCCEEEEEEECCCCCCCCEEEEECCCHHHHHHC
NRKISQLAAELKVANDKLFEQACTDPLTKLKNRRAFNEDLNRLIHLAQNQGTSLSLLMID
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEEEE
VDFFKDYNDNFGHPAGDKVLIEIALLLTRALRKCDVLARYGGEEFIIALPDASQTDSVEI
EHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
TERLIQTIREFSWEKRPITISIGISTLTFPLGGTLPIENNACLTNLIESADRALYASKTE
HHHHHHHHHHCCCCCCCEEEEEECEEEEECCCCEEECCCCHHHHHHHHHHHHHEEECCCC
GRDRFTHFTEIHSPDRLNLSSFQ
CCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 6094528; 7984428 [H]