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The map label for this gene is murI

Identifier: 116327584

GI number: 116327584

Start: 942249

End: 943025

Strand: Direct

Name: murI

Synonym: LBL_0815

Alternate gene names: 116327584

Gene position: 942249-943025 (Clockwise)

Preceding gene: 116327583

Following gene: 116327585

Centisome position: 26.07

GC content: 43.11

Gene sequence:

>777_bases
TTGAAAGGGCCGCTAAAGATCGGATTGATGGATTCCGGAATGGGCGGTTTGTCGGTACTTAAGGGAATTCTGAAATACGA
TGCGGAATTGGAGGTCGTATATTACGGAGATTTAAAGAACAGCCCTTACGGAGAAAAAGAAACCTCCGAAATTCTGGAAC
TTGTCCGGGACGTATGTAAGCGATTGCAAGAAGAAAACGTGAGCGCGATTTTGCTTGCTTGTAATACGGCTACGTCCGCC
GCCGCACAAACTCTTAGAAAGGAGTTTTCTATTCCTATATTCGGGATGGAACCGGCGATAAAACCGGCGATACTGCAGAA
TCCCGGAAAAAAAATCGCGCTCCTGGCAACTCCGGTCACACAAAGAGAGAAAAAATTACAAAGGCTCAAAGCGGAATTGG
GAGCGGAAGAATTGATTCTTCCCGTTTCTTGTCCCGGTCTAGCCGGCTTGGTGGATAAGGGAGAATTTGACGAGGCAGAG
AAATATCTTCGGCCTATTTTGAAAAAACTCCGGGAGGAGAATGTGGAGAACCTCGTTCTTGGATGTACACATTATATTTT
TTTAAAACATATAATTCTAAAAAACTTTCCGAATGTGAGGATTTACGACGGAAATTCTGGGACGATAAAACATCTATTGA
ATTCTTTACAAGTGCGTCAAAGAGTTTCGAATCGGAGTTCAGTTTCCGGATCCGTTTACAAGCTTATCTTAAACAGCGAT
GAGGAATTACACTTTCGATTGGCGACAGAACTTTTGCAATTCGAGAACAAGTTCTAA

Upstream 100 bases:

>100_bases
AAAGCGAAGAAAAATTCGATTGGATCTACAGGACGCTTCGAGATAGAATCGGCGAATATATCGCTTCCGTCTAAAATTAG
TTTCGATTGAGAGTTTACAA

Downstream 100 bases:

>100_bases
GTATAAATCACCTTCCAGAAAAGGATTCCGGCCTGATTTTTGAAAACCGATTCTATGGAAATAAATTTCTAAAGTGATTT
TTAAATTTTTTGAAATAAAT

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKGPLKIGLMDSGMGGLSVLKGILKYDAELEVVYYGDLKNSPYGEKETSEILELVRDVCKRLQEENVSAILLACNTATSA
AAQTLRKEFSIPIFGMEPAIKPAILQNPGKKIALLATPVTQREKKLQRLKAELGAEELILPVSCPGLAGLVDKGEFDEAE
KYLRPILKKLREENVENLVLGCTHYIFLKHIILKNFPNVRIYDGNSGTIKHLLNSLQVRQRVSNRSSVSGSVYKLILNSD
EELHFRLATELLQFENKF

Sequences:

>Translated_258_residues
MKGPLKIGLMDSGMGGLSVLKGILKYDAELEVVYYGDLKNSPYGEKETSEILELVRDVCKRLQEENVSAILLACNTATSA
AAQTLRKEFSIPIFGMEPAIKPAILQNPGKKIALLATPVTQREKKLQRLKAELGAEELILPVSCPGLAGLVDKGEFDEAE
KYLRPILKKLREENVENLVLGCTHYIFLKHIILKNFPNVRIYDGNSGTIKHLLNSLQVRQRVSNRSSVSGSVYKLILNSD
EELHFRLATELLQFENKF
>Mature_258_residues
MKGPLKIGLMDSGMGGLSVLKGILKYDAELEVVYYGDLKNSPYGEKETSEILELVRDVCKRLQEENVSAILLACNTATSA
AAQTLRKEFSIPIFGMEPAIKPAILQNPGKKIALLATPVTQREKKLQRLKAELGAEELILPVSCPGLAGLVDKGEFDEAE
KYLRPILKKLREENVENLVLGCTHYIFLKHIILKNFPNVRIYDGNSGTIKHLLNSLQVRQRVSNRSSVSGSVYKLILNSD
EELHFRLATELLQFENKF

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=187, Percent_Identity=33.6898395721925, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_LEPBJ (Q04QQ5)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801523.1
- ProteinModelPortal:   Q04QQ5
- SMR:   Q04QQ5
- STRING:   Q04QQ5
- GeneID:   4410957
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2292
- eggNOG:   COG0796
- HOGENOM:   HBG645102
- OMA:   ARTNAMI
- PhylomeDB:   Q04QQ5
- ProtClustDB:   CLSK574702
- BioCyc:   LBOR355277:LBJ_2292-MONOMER
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 28796; Mature: 28796

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGPLKIGLMDSGMGGLSVLKGILKYDAELEVVYYGDLKNSPYGEKETSEILELVRDVCK
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
RLQEENVSAILLACNTATSAAAQTLRKEFSIPIFGMEPAIKPAILQNPGKKIALLATPVT
HHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCEECCCCCCCCHHHCCCCCEEEEEECCHH
QREKKLQRLKAELGAEELILPVSCPGLAGLVDKGEFDEAEKYLRPILKKLREENVENLVL
HHHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
GCTHYIFLKHIILKNFPNVRIYDGNSGTIKHLLNSLQVRQRVSNRSSVSGSVYKLILNSD
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
EELHFRLATELLQFENKF
CHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKGPLKIGLMDSGMGGLSVLKGILKYDAELEVVYYGDLKNSPYGEKETSEILELVRDVCK
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
RLQEENVSAILLACNTATSAAAQTLRKEFSIPIFGMEPAIKPAILQNPGKKIALLATPVT
HHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCEECCCCCCCCHHHCCCCCEEEEEECCHH
QREKKLQRLKAELGAEELILPVSCPGLAGLVDKGEFDEAEKYLRPILKKLREENVENLVL
HHHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
GCTHYIFLKHIILKNFPNVRIYDGNSGTIKHLLNSLQVRQRVSNRSSVSGSVYKLILNSD
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
EELHFRLATELLQFENKF
CHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA