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The map label for this gene is rsbU [H]

Identifier: 116327562

GI number: 116327562

Start: 901672

End: 903435

Strand: Reverse

Name: rsbU [H]

Synonym: LBL_0791

Alternate gene names: 116327562

Gene position: 903435-901672 (Counterclockwise)

Preceding gene: 116327564

Following gene: 116327561

Centisome position: 25.0

GC content: 40.65

Gene sequence:

>1764_bases
ATGCCTCAAACCGTTTTAGAATCTCTAAAACTGAAAAGTATTCTCAGTACAAGCACGATTTTAAACGCAAATTTAGATCT
CTATCAGCTACTACCCCTAATCATGCTCTACTCCAAAGATCTTCTGGAGGCAGACGCGAGTTCCTTGTTCCTTCTCGACG
AAACGGAAGAATTTTTATACTGCGAAGTAGCGCTTGGTGAAAAAGGTGAAATCATTCAAAAATACGGGCGCCTCGATATA
GGAGAGGGAATCGCCGGTTGGGTGGCCAAAGAAAAAAAGCCGATCATTTTAGAAGACGCGTATTCGGATTCTAGATTCAA
TCCATCCTGGGATCAAAAGACGGGTTATAGAACCCGTTCCTTGGTTTGTGTTCCTTTGTTCATCGAAAACAAGATTATCG
GAACTCTTGAAATTCTCAATAAAACGCAAAACCGCTCGTTCAATGCTTGCGATTTGAACTACCTTACGTCCTTATCCGAA
GTGGCCGCAATCGCGATCCAAAACGCGAAGATTCATGAAAATCTTAAAAAAAGAATTTTGGAACTTTCTCTTCTTTACGA
ATTCGAAAAACTCATCGTTTCCGAAAAAAGCATTCATGAACTGGGCAGTTGGGTATTGGAAAGAGTTTTAGAATTCTTAG
AAGCAAAAACCGGAACCATTTATATCGCGGATCATACAAGTCAAACATTGAGAATCCTCGCAGCGAAAGGAATTCCAAAG
GACGCAATTCATACAATTATAGTTCCTTTCGGCGAAGGAGTTGCCGGCTGGGTGGCCAAGGAAAGAAAAAATCTTCTCAT
ACAAAACTTGGAAGAAGACAAACGTTATAATTTGAACGTGAAATACAAGTTCGAAGCGAACTCTCTTATTTCTTCTCCCT
TGATTTATAAAGATGAACTTCTAGGGGTTATCAGCGTCAATAACAAGAAGTCCGGATTTGCGTTCCATAAAAACGATCTA
GAAATGCTGGGGGCGATTGCAAACCGACTTTCAATGACGATCAAGAATGCGGACCTCTTTCATAAAGTCGTGAATTCCGA
TCGGGAATTACAAAGAGCCAGAGAAGTCATGTCGAAAGTAATTCCGACTACGATCCCTTATATTAAAGGTCTGGAAGTCG
GAGCGGAACACATTCCGTACTCGAACGTGGGTGGTGACTTTTATAGTATATTCAAATTGGATTCGGAACGAACAGGATTT
TTAGTCGCGGACGTTTCCGGACACGGTCTTTCCGCTTCGGTCATTGCGACAGTAATGAACACAATCATTTCCACATACGA
CAAAGAAATTCTTTCTAGTCCATCCCAGTTTTTTTCGGGGCTCAACCACGCCCTCAATAATAAAATGGTCGGGAATTTCG
TAACCGCATTTTACTGTGTGATCGACACGAAAAAAGATACGATTCTTTATTCCAATGCGGGACACAATTATCCTTTACTT
TTACAAAATTCGACCGATAGCATGATCTCTCTGGAAACGAAAGGAAAACTAATCGGTATTATTTCGGACCTTTTTTTTGA
GGAGAAATCCATTCATTTCAAAATCGGTGATAGGTTGGTGCTTTATACGGACGGAATTTCAGAGCATTATTCCGACGACA
GAACCAAACGTTACAGCGAAGAATTCATCTCTATTTCGATCCATAAGTGCGCCTCTAAAACCGCTCAGGAAACATCCGCA
CAAATTATTTCCGATTGTATCGAATATTGCAATTATTCCAAGTTCAGCGACGATGTAACTCTTCTAGTAATCGATCGCAA
ATAA

Upstream 100 bases:

>100_bases
TTCGGTTGGTATAGGCTTTTCTTCGGTATTTTCAATTCCTCGAATATTCCCTCAAACCCTTGACGAACATTCAGTTTCTC
TTAGTTTTGACGAAATATCT

Downstream 100 bases:

>100_bases
CGTGAATTCGATATAAGGATAGAATTCCTACTTCGTTCATTTGAAAGACAACATCATACGTCTTTATAATAAAATTTTTT
AAATCGAAATCGTTATGCTT

Product: Serine phosphatase RsbU, regulator of sigma subunit

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MPQTVLESLKLKSILSTSTILNANLDLYQLLPLIMLYSKDLLEADASSLFLLDETEEFLYCEVALGEKGEIIQKYGRLDI
GEGIAGWVAKEKKPIILEDAYSDSRFNPSWDQKTGYRTRSLVCVPLFIENKIIGTLEILNKTQNRSFNACDLNYLTSLSE
VAAIAIQNAKIHENLKKRILELSLLYEFEKLIVSEKSIHELGSWVLERVLEFLEAKTGTIYIADHTSQTLRILAAKGIPK
DAIHTIIVPFGEGVAGWVAKERKNLLIQNLEEDKRYNLNVKYKFEANSLISSPLIYKDELLGVISVNNKKSGFAFHKNDL
EMLGAIANRLSMTIKNADLFHKVVNSDRELQRAREVMSKVIPTTIPYIKGLEVGAEHIPYSNVGGDFYSIFKLDSERTGF
LVADVSGHGLSASVIATVMNTIISTYDKEILSSPSQFFSGLNHALNNKMVGNFVTAFYCVIDTKKDTILYSNAGHNYPLL
LQNSTDSMISLETKGKLIGIISDLFFEEKSIHFKIGDRLVLYTDGISEHYSDDRTKRYSEEFISISIHKCASKTAQETSA
QIISDCIEYCNYSKFSDDVTLLVIDRK

Sequences:

>Translated_587_residues
MPQTVLESLKLKSILSTSTILNANLDLYQLLPLIMLYSKDLLEADASSLFLLDETEEFLYCEVALGEKGEIIQKYGRLDI
GEGIAGWVAKEKKPIILEDAYSDSRFNPSWDQKTGYRTRSLVCVPLFIENKIIGTLEILNKTQNRSFNACDLNYLTSLSE
VAAIAIQNAKIHENLKKRILELSLLYEFEKLIVSEKSIHELGSWVLERVLEFLEAKTGTIYIADHTSQTLRILAAKGIPK
DAIHTIIVPFGEGVAGWVAKERKNLLIQNLEEDKRYNLNVKYKFEANSLISSPLIYKDELLGVISVNNKKSGFAFHKNDL
EMLGAIANRLSMTIKNADLFHKVVNSDRELQRAREVMSKVIPTTIPYIKGLEVGAEHIPYSNVGGDFYSIFKLDSERTGF
LVADVSGHGLSASVIATVMNTIISTYDKEILSSPSQFFSGLNHALNNKMVGNFVTAFYCVIDTKKDTILYSNAGHNYPLL
LQNSTDSMISLETKGKLIGIISDLFFEEKSIHFKIGDRLVLYTDGISEHYSDDRTKRYSEEFISISIHKCASKTAQETSA
QIISDCIEYCNYSKFSDDVTLLVIDRK
>Mature_586_residues
PQTVLESLKLKSILSTSTILNANLDLYQLLPLIMLYSKDLLEADASSLFLLDETEEFLYCEVALGEKGEIIQKYGRLDIG
EGIAGWVAKEKKPIILEDAYSDSRFNPSWDQKTGYRTRSLVCVPLFIENKIIGTLEILNKTQNRSFNACDLNYLTSLSEV
AAIAIQNAKIHENLKKRILELSLLYEFEKLIVSEKSIHELGSWVLERVLEFLEAKTGTIYIADHTSQTLRILAAKGIPKD
AIHTIIVPFGEGVAGWVAKERKNLLIQNLEEDKRYNLNVKYKFEANSLISSPLIYKDELLGVISVNNKKSGFAFHKNDLE
MLGAIANRLSMTIKNADLFHKVVNSDRELQRAREVMSKVIPTTIPYIKGLEVGAEHIPYSNVGGDFYSIFKLDSERTGFL
VADVSGHGLSASVIATVMNTIISTYDKEILSSPSQFFSGLNHALNNKMVGNFVTAFYCVIDTKKDTILYSNAGHNYPLLL
QNSTDSMISLETKGKLIGIISDLFFEEKSIHFKIGDRLVLYTDGISEHYSDDRTKRYSEEFISISIHKCASKTAQETSAQ
IISDCIEYCNYSKFSDDVTLLVIDRK

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

Organism=Homo sapiens, GI5729972, Length=153, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI194595488, Length=181, Percent_Identity=32.5966850828729, Blast_Score=91, Evalue=5e-18,
Organism=Homo sapiens, GI61744435, Length=357, Percent_Identity=24.6498599439776, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI61744430, Length=357, Percent_Identity=24.6498599439776, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI61744432, Length=357, Percent_Identity=24.6498599439776, Blast_Score=82, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193202333, Length=152, Percent_Identity=30.9210526315789, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI161076953, Length=332, Percent_Identity=26.2048192771084, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI161076955, Length=332, Percent_Identity=26.2048192771084, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281361762, Length=216, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 65997; Mature: 65865

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQTVLESLKLKSILSTSTILNANLDLYQLLPLIMLYSKDLLEADASSLFLLDETEEFLY
CCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEE
CEVALGEKGEIIQKYGRLDIGEGIAGWVAKEKKPIILEDAYSDSRFNPSWDQKTGYRTRS
EEEEECCCCHHHHHHCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCE
LVCVPLFIENKIIGTLEILNKTQNRSFNACDLNYLTSLSEVAAIAIQNAKIHENLKKRIL
EEEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ELSLLYEFEKLIVSEKSIHELGSWVLERVLEFLEAKTGTIYIADHTSQTLRILAAKGIPK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEHEEEECCCCH
DAIHTIIVPFGEGVAGWVAKERKNLLIQNLEEDKRYNLNVKYKFEANSLISSPLIYKDEL
HHHEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEECEEEEEECCCCHHCCCCEEHHHE
LGVISVNNKKSGFAFHKNDLEMLGAIANRLSMTIKNADLFHKVVNSDRELQRAREVMSKV
EEEEEECCCCCCCEEECCHHHHHHHHHHHHHEEECCHHHHHHHHCCHHHHHHHHHHHHHH
IPTTIPYIKGLEVGAEHIPYSNVGGDFYSIFKLDSERTGFLVADVSGHGLSASVIATVMN
CCCCCCHHCCCCCCHHHCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHH
TIISTYDKEILSSPSQFFSGLNHALNNKMVGNFVTAFYCVIDTKKDTILYSNAGHNYPLL
HHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHEEEEEEEECCCCEEEEECCCCCCCEE
LQNSTDSMISLETKGKLIGIISDLFFEEKSIHFKIGDRLVLYTDGISEHYSDDRTKRYSE
EECCCCCEEEEECCCCEEHHHHHHHHCCCCEEEEECCEEEEEECCCHHHCCCHHHHHHHH
EFISISIHKCASKTAQETSAQIISDCIEYCNYSKFSDDVTLLVIDRK
HHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
PQTVLESLKLKSILSTSTILNANLDLYQLLPLIMLYSKDLLEADASSLFLLDETEEFLY
CHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEE
CEVALGEKGEIIQKYGRLDIGEGIAGWVAKEKKPIILEDAYSDSRFNPSWDQKTGYRTRS
EEEEECCCCHHHHHHCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCE
LVCVPLFIENKIIGTLEILNKTQNRSFNACDLNYLTSLSEVAAIAIQNAKIHENLKKRIL
EEEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ELSLLYEFEKLIVSEKSIHELGSWVLERVLEFLEAKTGTIYIADHTSQTLRILAAKGIPK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEHEEEECCCCH
DAIHTIIVPFGEGVAGWVAKERKNLLIQNLEEDKRYNLNVKYKFEANSLISSPLIYKDEL
HHHEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEECEEEEEECCCCHHCCCCEEHHHE
LGVISVNNKKSGFAFHKNDLEMLGAIANRLSMTIKNADLFHKVVNSDRELQRAREVMSKV
EEEEEECCCCCCCEEECCHHHHHHHHHHHHHEEECCHHHHHHHHCCHHHHHHHHHHHHHH
IPTTIPYIKGLEVGAEHIPYSNVGGDFYSIFKLDSERTGFLVADVSGHGLSASVIATVMN
CCCCCCHHCCCCCCHHHCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHH
TIISTYDKEILSSPSQFFSGLNHALNNKMVGNFVTAFYCVIDTKKDTILYSNAGHNYPLL
HHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHEEEEEEEECCCCEEEEECCCCCCCEE
LQNSTDSMISLETKGKLIGIISDLFFEEKSIHFKIGDRLVLYTDGISEHYSDDRTKRYSE
EECCCCCEEEEECCCCEEHHHHHHHHCCCCEEEEECCEEEEEECCCHHHCCCHHHHHHHH
EFISISIHKCASKTAQETSAQIISDCIEYCNYSKFSDDVTLLVIDRK
HHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]