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The map label for this gene is proB [H]

Identifier: 116327411

GI number: 116327411

Start: 693643

End: 694509

Strand: Reverse

Name: proB [H]

Synonym: LBL_0620

Alternate gene names: 116327411

Gene position: 694509-693643 (Counterclockwise)

Preceding gene: 116327412

Following gene: 116327410

Centisome position: 19.21

GC content: 43.94

Gene sequence:

>867_bases
ATGGAACGAAATTCACTGTCAGAAAAAATTCACAATGCGGAGATGATCGTCGTCAAAGTAGGTTCCGCGAGGTTGTCCGG
TCCTGAGCCGGAAGTGAACGATTTTCTATTTCAATTGGTAAGCGACATTCGTCATCTCAGAGACCTCGGTAAAAAAGTGA
TCCTAGTTTCCTCCGGAGCGATCGCAAGAGGAAAGCTTTTGTTAAACGAACTCCCCTCGTCCATTTCTTCTAGAGATTCT
CTCTCCGAAAAACAAGCGTTAGCCGCTATGGGTCAAAACAGACTCATCAATCTTTACGATAGTTTTTTTTCCAAGGTGAA
CTTGAGCATAGCCCAGATTCTTTTCGGCGTTCTGGATCTTGAATCCAAAGAAGGTTATAAAAATCTAAAGAATACGTTCA
CTCAACTTTTGGAATGGGGAATACTTCCTATTGTCAACGAGAACGACTCGGTCGCAACCGAAGAAGTGAAATTCGGAGAC
AACGATATGCTTTCCGCACTTGTAAGTTTAATCGTAGGTGCGGATTTGCTCCTCATTTTAACCGGAGTGGAGGGATTTCT
CAAAGAAGAAAAAGTAGTCCCTTTTTTAGAGGGAATTTCCAAAGACGATCTGAACCTCGCGGGCGGCCCGAGCGGTCCTG
GAACCGGCGGAATGTTCACCAAACTTAAATCAGCCGGCCTATTGTCGGAAGCAGGAATACCGACCGCAATCTTAAACGGC
AAGAAAATACACGCGATCCGAGAATTTTTGGAAAAAAACTCGGTCGGAACTTTGATTGCACCTTCCGGAAACCGAGTTTT
TTCCGAAGAAGATGTGAAAGAAATCATTCGCAAAAATCGAAACGGCAATGGGGAAAATCATTTATGA

Upstream 100 bases:

>100_bases
TCCTTTAAATTTTTGAGACAAAGACTTGCAGTGCAAAAACGGACTTCGTAAAAGTAGATTCAAAAAAACTTTTCCGACTA
CAATCAACTTACAAAATACA

Downstream 100 bases:

>100_bases
AAGAAATCGAATACGTTAAAGAACTTTCTTATCGGGCCAAAAAAGCTTCCAGAACTTTAAAATCACTCTCTTCATTTCAA
AAAAACAAGGTTCTTTTGGA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MERNSLSEKIHNAEMIVVKVGSARLSGPEPEVNDFLFQLVSDIRHLRDLGKKVILVSSGAIARGKLLLNELPSSISSRDS
LSEKQALAAMGQNRLINLYDSFFSKVNLSIAQILFGVLDLESKEGYKNLKNTFTQLLEWGILPIVNENDSVATEEVKFGD
NDMLSALVSLIVGADLLLILTGVEGFLKEEKVVPFLEGISKDDLNLAGGPSGPGTGGMFTKLKSAGLLSEAGIPTAILNG
KKIHAIREFLEKNSVGTLIAPSGNRVFSEEDVKEIIRKNRNGNGENHL

Sequences:

>Translated_288_residues
MERNSLSEKIHNAEMIVVKVGSARLSGPEPEVNDFLFQLVSDIRHLRDLGKKVILVSSGAIARGKLLLNELPSSISSRDS
LSEKQALAAMGQNRLINLYDSFFSKVNLSIAQILFGVLDLESKEGYKNLKNTFTQLLEWGILPIVNENDSVATEEVKFGD
NDMLSALVSLIVGADLLLILTGVEGFLKEEKVVPFLEGISKDDLNLAGGPSGPGTGGMFTKLKSAGLLSEAGIPTAILNG
KKIHAIREFLEKNSVGTLIAPSGNRVFSEEDVKEIIRKNRNGNGENHL
>Mature_288_residues
MERNSLSEKIHNAEMIVVKVGSARLSGPEPEVNDFLFQLVSDIRHLRDLGKKVILVSSGAIARGKLLLNELPSSISSRDS
LSEKQALAAMGQNRLINLYDSFFSKVNLSIAQILFGVLDLESKEGYKNLKNTFTQLLEWGILPIVNENDSVATEEVKFGD
NDMLSALVSLIVGADLLLILTGVEGFLKEEKVVPFLEGISKDDLNLAGGPSGPGTGGMFTKLKSAGLLSEAGIPTAILNG
KKIHAIREFLEKNSVGTLIAPSGNRVFSEEDVKEIIRKNRNGNGENHL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=297, Percent_Identity=30.976430976431, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI21361368, Length=299, Percent_Identity=30.7692307692308, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786437, Length=258, Percent_Identity=39.5348837209302, Blast_Score=164, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI17569967, Length=302, Percent_Identity=32.1192052980132, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17569969, Length=302, Percent_Identity=32.1192052980132, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320506, Length=240, Percent_Identity=41.6666666666667, Blast_Score=146, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321822, Length=244, Percent_Identity=40.1639344262295, Blast_Score=142, Evalue=6e-35,
Organism=Drosophila melanogaster, GI21357643, Length=286, Percent_Identity=32.1678321678322, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 31210; Mature: 31210

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00902 GLUTAMATE_5_KINASE ; PS00327 BACTERIAL_OPSIN_RET

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERNSLSEKIHNAEMIVVKVGSARLSGPEPEVNDFLFQLVSDIRHLRDLGKKVILVSSGA
CCCHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
IARGKLLLNELPSSISSRDSLSEKQALAAMGQNRLINLYDSFFSKVNLSIAQILFGVLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
ESKEGYKNLKNTFTQLLEWGILPIVNENDSVATEEVKFGDNDMLSALVSLIVGADLLLIL
CCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TGVEGFLKEEKVVPFLEGISKDDLNLAGGPSGPGTGGMFTKLKSAGLLSEAGIPTAILNG
HHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHCCCCHHHCCC
KKIHAIREFLEKNSVGTLIAPSGNRVFSEEDVKEIIRKNRNGNGENHL
CHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MERNSLSEKIHNAEMIVVKVGSARLSGPEPEVNDFLFQLVSDIRHLRDLGKKVILVSSGA
CCCHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
IARGKLLLNELPSSISSRDSLSEKQALAAMGQNRLINLYDSFFSKVNLSIAQILFGVLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
ESKEGYKNLKNTFTQLLEWGILPIVNENDSVATEEVKFGDNDMLSALVSLIVGADLLLIL
CCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TGVEGFLKEEKVVPFLEGISKDDLNLAGGPSGPGTGGMFTKLKSAGLLSEAGIPTAILNG
HHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHCCCCHHHCCC
KKIHAIREFLEKNSVGTLIAPSGNRVFSEEDVKEIIRKNRNGNGENHL
CHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA