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The map label for this gene is proA

Identifier: 116327410

GI number: 116327410

Start: 692396

End: 693646

Strand: Reverse

Name: proA

Synonym: LBL_0619

Alternate gene names: 116327410

Gene position: 693646-692396 (Counterclockwise)

Preceding gene: 116327411

Following gene: 116327409

Centisome position: 19.19

GC content: 44.04

Gene sequence:

>1251_bases
ATGAAAGAAATCGAATACGTTAAAGAACTTTCTTATCGGGCCAAAAAAGCTTCCAGAACTTTAAAATCACTCTCTTCATT
TCAAAAAAACAAGGTTCTTTTGGAACTTGCAAACCTATTAGAAAAACGTAAATCGGAAATCCTTTCCGCAAACGAGTTCG
ATCTAAAGAACGGCAAAGAAAAGAATCTCCCTTCCGCTTTGATGGATCGTCTTTTATTAAATGAGAAACGAATCGATTCC
ATGGCTTTCGCAGTTCGAGAAATCGTTTCACTTCCAGACCCAGTCGGAGAAGTTACGAGAGGTTTGACCCTTCCGAACGG
TCTCGAACTTGTGACCAAAAGAGTTCCTCTCGGAGTCGTGATGGTCATCTACGAATCTCGTCCGAACGTAACGATCGACG
TAGGCGCCCTTTCTTTCAAATCGGGGAACGCATGTATTCTCAGAGGGGGAAGCGAAGCGTTTCATTCCAATGAAATTTTA
GTAAAACTATTTCATGAAATTCTAAATAAAGAAGGAATAGACACAAGTGCGATCACTTTCGTGGACAAAACGGACCGCTC
CTTTATGCTTCCTTTCCTTCAACAGACTCCTTCGATCGACGTAGTGGTTCCGAGAGGCGGAGAAGGTTTGATCAAATTCA
TCTCGGAACATTCCATGATTCCAGTCGTCAAACATGATAAAGGTGTTTGTAACCTCTATATCGATCAGGATGCCGACCCT
GCAAAAGTGATTCCGATCGTAATCAACTCCAAGGTGCAAAGACCGGGAGTATGCAACGCTACGGAAAATTTAATACTTCA
CAACGGTTATCCATTTCGGAAAGAATTGTTAGAGGCCCTAGCAAAAGAAGGTGTGGAGCTATTACTTGATCCTTCCGCGC
TTTCTCTCTACCCGAAAGGAAGACCGGTTAAAGAAGAAGACTATCAGGAAGAATTTTTGGATCTTCGTCTTTCGGTAAAA
ACGGTTTCCAGCCTGGAAGAAGCGTTAACCTTTATCGAAAGAACTTCTTCAGGTCATACGGAAGCCATCATCACGGAAGA
TTTAAACGCGGCTAAAATGTTCACAAATTCTTTGGACTCCGCCGCTTTATTTGTCAATTGCTCCACCCGTTTTCACGACG
GCGCGGAATTCGGTCTCGGCGCCGAAGTGGGAATCTCCACCGGCAAGTTACACGTCCGCGGTCCTATGGGTTTGGTCCAT
CTCACGACCACAACCACCTACGTAACCGGAAACGGACAAATCCGAAATTAA

Upstream 100 bases:

>100_bases
TCGGAACTTTGATTGCACCTTCCGGAAACCGAGTTTTTTCCGAAGAAGATGTGAAAGAAATCATTCGCAAAAATCGAAAC
GGCAATGGGGAAAATCATTT

Downstream 100 bases:

>100_bases
CCTATGACTTCACCAATCCTCACTGGAATTTTCGGAGGCAGTTTCGATCCTCCCCACGAAGGACATTCGGGAATTTTAAA
GTCTTTTTTTCGCGAAGTCC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKEIEYVKELSYRAKKASRTLKSLSSFQKNKVLLELANLLEKRKSEILSANEFDLKNGKEKNLPSALMDRLLLNEKRIDS
MAFAVREIVSLPDPVGEVTRGLTLPNGLELVTKRVPLGVVMVIYESRPNVTIDVGALSFKSGNACILRGGSEAFHSNEIL
VKLFHEILNKEGIDTSAITFVDKTDRSFMLPFLQQTPSIDVVVPRGGEGLIKFISEHSMIPVVKHDKGVCNLYIDQDADP
AKVIPIVINSKVQRPGVCNATENLILHNGYPFRKELLEALAKEGVELLLDPSALSLYPKGRPVKEEDYQEEFLDLRLSVK
TVSSLEEALTFIERTSSGHTEAIITEDLNAAKMFTNSLDSAALFVNCSTRFHDGAEFGLGAEVGISTGKLHVRGPMGLVH
LTTTTTYVTGNGQIRN

Sequences:

>Translated_416_residues
MKEIEYVKELSYRAKKASRTLKSLSSFQKNKVLLELANLLEKRKSEILSANEFDLKNGKEKNLPSALMDRLLLNEKRIDS
MAFAVREIVSLPDPVGEVTRGLTLPNGLELVTKRVPLGVVMVIYESRPNVTIDVGALSFKSGNACILRGGSEAFHSNEIL
VKLFHEILNKEGIDTSAITFVDKTDRSFMLPFLQQTPSIDVVVPRGGEGLIKFISEHSMIPVVKHDKGVCNLYIDQDADP
AKVIPIVINSKVQRPGVCNATENLILHNGYPFRKELLEALAKEGVELLLDPSALSLYPKGRPVKEEDYQEEFLDLRLSVK
TVSSLEEALTFIERTSSGHTEAIITEDLNAAKMFTNSLDSAALFVNCSTRFHDGAEFGLGAEVGISTGKLHVRGPMGLVH
LTTTTTYVTGNGQIRN
>Mature_416_residues
MKEIEYVKELSYRAKKASRTLKSLSSFQKNKVLLELANLLEKRKSEILSANEFDLKNGKEKNLPSALMDRLLLNEKRIDS
MAFAVREIVSLPDPVGEVTRGLTLPNGLELVTKRVPLGVVMVIYESRPNVTIDVGALSFKSGNACILRGGSEAFHSNEIL
VKLFHEILNKEGIDTSAITFVDKTDRSFMLPFLQQTPSIDVVVPRGGEGLIKFISEHSMIPVVKHDKGVCNLYIDQDADP
AKVIPIVINSKVQRPGVCNATENLILHNGYPFRKELLEALAKEGVELLLDPSALSLYPKGRPVKEEDYQEEFLDLRLSVK
TVSSLEEALTFIERTSSGHTEAIITEDLNAAKMFTNSLDSAALFVNCSTRFHDGAEFGLGAEVGISTGKLHVRGPMGLVH
LTTTTTYVTGNGQIRN

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=413, Percent_Identity=37.5302663438257, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI62912457, Length=413, Percent_Identity=37.5302663438257, Blast_Score=262, Evalue=5e-70,
Organism=Escherichia coli, GI1786438, Length=415, Percent_Identity=42.4096385542169, Blast_Score=331, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI17569967, Length=414, Percent_Identity=38.6473429951691, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI17569969, Length=414, Percent_Identity=38.6473429951691, Blast_Score=266, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324899, Length=429, Percent_Identity=34.7319347319347, Blast_Score=259, Evalue=5e-70,
Organism=Drosophila melanogaster, GI21357643, Length=411, Percent_Identity=36.9829683698297, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_LEPBJ (Q04Q92)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801686.1
- ProteinModelPortal:   Q04Q92
- SMR:   Q04Q92
- STRING:   Q04Q92
- GeneID:   4412100
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2490
- eggNOG:   COG0014
- HOGENOM:   HBG318080
- OMA:   HRIEAMA
- PhylomeDB:   Q04Q92
- ProtClustDB:   PRK00197
- BioCyc:   LBOR355277:LBJ_2490-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45910; Mature: 45910

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIEYVKELSYRAKKASRTLKSLSSFQKNKVLLELANLLEKRKSEILSANEFDLKNGKE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
KNLPSALMDRLLLNEKRIDSMAFAVREIVSLPDPVGEVTRGLTLPNGLELVTKRVPLGVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHCCCEEE
MVIYESRPNVTIDVGALSFKSGNACILRGGSEAFHSNEILVKLFHEILNKEGIDTSAITF
EEEEECCCCEEEEEEEEEECCCCEEEEECCCHHHCCCHHHHHHHHHHHCCCCCCCCEEEE
VDKTDRSFMLPFLQQTPSIDVVVPRGGEGLIKFISEHSMIPVVKHDKGVCNLYIDQDADP
EECCCCCEECHHHHCCCCEEEEEECCCCHHHHHHHCCCCCEEEECCCCEEEEEECCCCCC
AKVIPIVINSKVQRPGVCNATENLILHNGYPFRKELLEALAKEGVELLLDPSALSLYPKG
CEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEECCCCEEECCCC
RPVKEEDYQEEFLDLRLSVKTVSSLEEALTFIERTSSGHTEAIITEDLNAAKMFTNSLDS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCC
AALFVNCSTRFHDGAEFGLGAEVGISTGKLHVRGPMGLVHLTTTTTYVTGNGQIRN
EEEEEEECCCCCCCCCCCCCCEECCCCCEEEEECCCEEEEEEEEEEEEECCCEECC
>Mature Secondary Structure
MKEIEYVKELSYRAKKASRTLKSLSSFQKNKVLLELANLLEKRKSEILSANEFDLKNGKE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
KNLPSALMDRLLLNEKRIDSMAFAVREIVSLPDPVGEVTRGLTLPNGLELVTKRVPLGVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHCCCEEE
MVIYESRPNVTIDVGALSFKSGNACILRGGSEAFHSNEILVKLFHEILNKEGIDTSAITF
EEEEECCCCEEEEEEEEEECCCCEEEEECCCHHHCCCHHHHHHHHHHHCCCCCCCCEEEE
VDKTDRSFMLPFLQQTPSIDVVVPRGGEGLIKFISEHSMIPVVKHDKGVCNLYIDQDADP
EECCCCCEECHHHHCCCCEEEEEECCCCHHHHHHHCCCCCEEEECCCCEEEEEECCCCCC
AKVIPIVINSKVQRPGVCNATENLILHNGYPFRKELLEALAKEGVELLLDPSALSLYPKG
CEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEECCCCEEECCCC
RPVKEEDYQEEFLDLRLSVKTVSSLEEALTFIERTSSGHTEAIITEDLNAAKMFTNSLDS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCC
AALFVNCSTRFHDGAEFGLGAEVGISTGKLHVRGPMGLVHLTTTTTYVTGNGQIRN
EEEEEEECCCCCCCCCCCCCCEECCCCCEEEEECCCEEEEEEEEEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA