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The map label for this gene is pth-2 [H]

Identifier: 116327329

GI number: 116327329

Start: 576668

End: 577231

Strand: Reverse

Name: pth-2 [H]

Synonym: LBL_0523

Alternate gene names: 116327329

Gene position: 577231-576668 (Counterclockwise)

Preceding gene: 116327330

Following gene: 116327328

Centisome position: 15.97

GC content: 40.78

Gene sequence:

>564_bases
ATGAAGCTGATCGTCGGACTCGGGAATCCAGGGGACAGATACAATAACAACCGCTCAAACATCGGTTTCAAAATTCTAGA
TGTTATCGCAAATAACATCAATGTTGAAATTAAGACCAAAAAGAAGAAGTCTCTGATTGGTCGCGGTGATTTTGAGGGAG
AAGAGGTCGTACTTTTAAAACCTCAGACGTTTAGCGATCTTTCGGGAGAATCCGTTTTATACATAGCGTCGTTTCTAAAA
ATCCAAGTGGGAGAAATTCTAGTCATTCAAGAAGATTGGACTCTGACACTCGGAAGAATCGTAGTCGATAAGGGAACTCA
AGAGACGGATCATCCCGGAGTAAAATCCATCGTTCAATCCCTTCGTTCTCCGAATTTCATTCGAATTCGAATCGGCATTC
AAAACGATGAGTTTAATCTGAAAACTCGGGATTCTTTTTTGAAGGAAGATTTCGAGCCGATGGAAAACTTAAGTCTGATT
CAGATCATCAATGATGCGGAAGCGGCGATTCGTTCGATTTCTCTAGGAGACATCGACGACGTAATCGAAAAATATCATCT
TTAA

Upstream 100 bases:

>100_bases
AAGCAAAAGGCGCCGTCCCAGACGACGCAAAAGCTAAAAAAGGTAAGAAGTAATACGAAACTTCCATCAGATCTAATCTG
ATAAAAACAGATAGGTATTC

Downstream 100 bases:

>100_bases
TATAGGAAATTTCTCGAATTTTCATTACTTCGAATTCCTTTTAACGGTTTCGATCAATTCTTCCTTGCCTTTTTAGAGAA
ACATGAAATTCTATCGGAAG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MKLIVGLGNPGDRYNNNRSNIGFKILDVIANNINVEIKTKKKKSLIGRGDFEGEEVVLLKPQTFSDLSGESVLYIASFLK
IQVGEILVIQEDWTLTLGRIVVDKGTQETDHPGVKSIVQSLRSPNFIRIRIGIQNDEFNLKTRDSFLKEDFEPMENLSLI
QIINDAEAAIRSISLGDIDDVIEKYHL

Sequences:

>Translated_187_residues
MKLIVGLGNPGDRYNNNRSNIGFKILDVIANNINVEIKTKKKKSLIGRGDFEGEEVVLLKPQTFSDLSGESVLYIASFLK
IQVGEILVIQEDWTLTLGRIVVDKGTQETDHPGVKSIVQSLRSPNFIRIRIGIQNDEFNLKTRDSFLKEDFEPMENLSLI
QIINDAEAAIRSISLGDIDDVIEKYHL
>Mature_187_residues
MKLIVGLGNPGDRYNNNRSNIGFKILDVIANNINVEIKTKKKKSLIGRGDFEGEEVVLLKPQTFSDLSGESVLYIASFLK
IQVGEILVIQEDWTLTLGRIVVDKGTQETDHPGVKSIVQSLRSPNFIRIRIGIQNDEFNLKTRDSFLKEDFEPMENLSLI
QIINDAEAAIRSISLGDIDDVIEKYHL

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Escherichia coli, GI1787455, Length=135, Percent_Identity=39.2592592592593, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 21041; Mature: 21041

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIVGLGNPGDRYNNNRSNIGFKILDVIANNINVEIKTKKKKSLIGRGDFEGEEVVLLK
CEEEEECCCCCCCCCCCCCCCCCEEEEEHHCCCEEEEEECHHHHCCCCCCCCCCEEEEEC
PQTFSDLSGESVLYIASFLKIQVGEILVIQEDWTLTLGRIVVDKGTQETDHPGVKSIVQS
CCCCCCCCCCCHHEEHHHHHEEECEEEEEECCCEEEEEEEEEECCCCCCCCCCHHHHHHH
LRSPNFIRIRIGIQNDEFNLKTRDSFLKEDFEPMENLSLIQIINDAEAAIRSISLGDIDD
HCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCHHHCCEEEEEECHHHHHHHHCCCCCHHH
VIEKYHL
HHHHHCC
>Mature Secondary Structure
MKLIVGLGNPGDRYNNNRSNIGFKILDVIANNINVEIKTKKKKSLIGRGDFEGEEVVLLK
CEEEEECCCCCCCCCCCCCCCCCEEEEEHHCCCEEEEEECHHHHCCCCCCCCCCEEEEEC
PQTFSDLSGESVLYIASFLKIQVGEILVIQEDWTLTLGRIVVDKGTQETDHPGVKSIVQS
CCCCCCCCCCCHHEEHHHHHEEECEEEEEECCCEEEEEEEEEECCCCCCCCCCHHHHHHH
LRSPNFIRIRIGIQNDEFNLKTRDSFLKEDFEPMENLSLIQIINDAEAAIRSISLGDIDD
HCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCHHHCCEEEEEECHHHHHHHHCCCCCHHH
VIEKYHL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA