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The map label for this gene is ispA-2 [H]

Identifier: 116327301

GI number: 116327301

Start: 540562

End: 541521

Strand: Reverse

Name: ispA-2 [H]

Synonym: LBL_0491

Alternate gene names: 116327301

Gene position: 541521-540562 (Counterclockwise)

Preceding gene: 116327302

Following gene: 116327300

Centisome position: 14.98

GC content: 41.88

Gene sequence:

>960_bases
GTGAAAGCGTCCGTACTAAAAGATTCTCTAATTCGGAAATTCGATAAAAAACTCGAAACGATCATTCGGGAAGATCTCAA
AATTCTTTCCGAAATTAAAACTTACACCATCCGATCCGGTGGGAAACGACTTCGCCCGATCCTTCACTATTGCCTTTGCC
AACTTTTAGGTTACTCCGGCAAACACTGGTTGGACGTAGGTGCAATCGTGGAACTGATTCACGCCGCTAGTTTACTTCAC
GACGATGTAGTCGACGAGGCGGAAACCAGAAGAGGACAACAAAGTGTGGGTTCCAAATTCGGGAACAAGACGGCGATTCT
TGCGGGAGATTACCTCCTTGCGTGCGGGATCGATCACCTAAACGACCTGGGCTATCCGGAACTAATGGACATTTTCACTC
AGGTAATCAAGGATCTTTCCATTTCCGAACTCATTCAGATGGAATGGGAAAAAAATCCGAAAATCACTTTAGATATTTAT
AATAAAATCGCCTACGGGAAAACCTCCTCCTTGTTCGGAGCAGTGGGGGTTTCTGCCGGAATTCTTTCGGATAAAAACGA
TAAGGAAAAAAAGAAGCTGAGACAATTCGGAATCGACCTCGGTTCTTTCTTTCAGAAAAAAGACGATGCGATTGATTATT
TCACTCCCGCGAGCAAAAGCGGGAAAGTTCCTCTCAAGGATTTTTACAACGGTCTTTATACCTATCCGATTTTGCTGTTA
CTCGATCAGGCGGATAAGAACGACAAAAAACTGATTCATTCACTTTTTGCCAAACCGGAAAGGTCGCAAGGGGACGAGGT
CGTGATTTTGAGTCTTCTTTCCCGTTATCAGATTCGAAAACAAATGGATAAGGAATTTCAAACGATTGTTAATAACTTAG
TGAAATTTTTGAATAGTTTTCCCGAATCTTCGATCCGTAATCTTTTAAAAGAACAAATTCTGAAACTTTTAGAAGAATAG

Upstream 100 bases:

>100_bases
TCCTGATTTTGGAAAACAAACTCAAAGCTTAGCCCCCTTTCAAGAGTTCTTGCTTGAGCCTGTACCGATATTTTGAATAC
TGGCTCCACGGGAAGGAGTA

Downstream 100 bases:

>100_bases
ATTTGCACTCCGGCCAATAAGGGTATTTACTGATTTCTCATGTGATTTCCTAAATGCAGAATCGAAATAAGGAATCAACC
TGAATCCTTTTTCGTACCTC

Product: geranyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKASVLKDSLIRKFDKKLETIIREDLKILSEIKTYTIRSGGKRLRPILHYCLCQLLGYSGKHWLDVGAIVELIHAASLLH
DDVVDEAETRRGQQSVGSKFGNKTAILAGDYLLACGIDHLNDLGYPELMDIFTQVIKDLSISELIQMEWEKNPKITLDIY
NKIAYGKTSSLFGAVGVSAGILSDKNDKEKKKLRQFGIDLGSFFQKKDDAIDYFTPASKSGKVPLKDFYNGLYTYPILLL
LDQADKNDKKLIHSLFAKPERSQGDEVVILSLLSRYQIRKQMDKEFQTIVNNLVKFLNSFPESSIRNLLKEQILKLLEE

Sequences:

>Translated_319_residues
MKASVLKDSLIRKFDKKLETIIREDLKILSEIKTYTIRSGGKRLRPILHYCLCQLLGYSGKHWLDVGAIVELIHAASLLH
DDVVDEAETRRGQQSVGSKFGNKTAILAGDYLLACGIDHLNDLGYPELMDIFTQVIKDLSISELIQMEWEKNPKITLDIY
NKIAYGKTSSLFGAVGVSAGILSDKNDKEKKKLRQFGIDLGSFFQKKDDAIDYFTPASKSGKVPLKDFYNGLYTYPILLL
LDQADKNDKKLIHSLFAKPERSQGDEVVILSLLSRYQIRKQMDKEFQTIVNNLVKFLNSFPESSIRNLLKEQILKLLEE
>Mature_319_residues
MKASVLKDSLIRKFDKKLETIIREDLKILSEIKTYTIRSGGKRLRPILHYCLCQLLGYSGKHWLDVGAIVELIHAASLLH
DDVVDEAETRRGQQSVGSKFGNKTAILAGDYLLACGIDHLNDLGYPELMDIFTQVIKDLSISELIQMEWEKNPKITLDIY
NKIAYGKTSSLFGAVGVSAGILSDKNDKEKKKLRQFGIDLGSFFQKKDDAIDYFTPASKSGKVPLKDFYNGLYTYPILLL
LDQADKNDKKLIHSLFAKPERSQGDEVVILSLLSRYQIRKQMDKEFQTIVNNLVKFLNSFPESSIRNLLKEQILKLLEE

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=233, Percent_Identity=31.7596566523605, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI4758430, Length=259, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI83700220, Length=259, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1789578, Length=233, Percent_Identity=30.4721030042918, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1786623, Length=266, Percent_Identity=31.203007518797, Blast_Score=88, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=275, Percent_Identity=32, Blast_Score=131, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6319475, Length=221, Percent_Identity=29.8642533936652, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24651612, Length=223, Percent_Identity=27.3542600896861, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI281365769, Length=234, Percent_Identity=25.6410256410256, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24660002, Length=234, Percent_Identity=25.6410256410256, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36236; Mature: 36236

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASVLKDSLIRKFDKKLETIIREDLKILSEIKTYTIRSGGKRLRPILHYCLCQLLGYSG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
KHWLDVGAIVELIHAASLLHDDVVDEAETRRGQQSVGSKFGNKTAILAGDYLLACGIDHL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCHHH
NDLGYPELMDIFTQVIKDLSISELIQMEWEKNPKITLDIYNKIAYGKTSSLFGAVGVSAG
HCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHH
ILSDKNDKEKKKLRQFGIDLGSFFQKKDDAIDYFTPASKSGKVPLKDFYNGLYTYPILLL
HCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LDQADKNDKKLIHSLFAKPERSQGDEVVILSLLSRYQIRKQMDKEFQTIVNNLVKFLNSF
HCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PESSIRNLLKEQILKLLEE
CHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKASVLKDSLIRKFDKKLETIIREDLKILSEIKTYTIRSGGKRLRPILHYCLCQLLGYSG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
KHWLDVGAIVELIHAASLLHDDVVDEAETRRGQQSVGSKFGNKTAILAGDYLLACGIDHL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCHHH
NDLGYPELMDIFTQVIKDLSISELIQMEWEKNPKITLDIYNKIAYGKTSSLFGAVGVSAG
HCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHH
ILSDKNDKEKKKLRQFGIDLGSFFQKKDDAIDYFTPASKSGKVPLKDFYNGLYTYPILLL
HCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LDQADKNDKKLIHSLFAKPERSQGDEVVILSLLSRYQIRKQMDKEFQTIVNNLVKFLNSF
HCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PESSIRNLLKEQILKLLEE
CHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]