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The map label for this gene is zraS [H]

Identifier: 116327190

GI number: 116327190

Start: 435780

End: 438143

Strand: Direct

Name: zraS [H]

Synonym: LBL_0372

Alternate gene names: 116327190

Gene position: 435780-438143 (Clockwise)

Preceding gene: 116327188

Following gene: 116327191

Centisome position: 12.06

GC content: 40.99

Gene sequence:

>2364_bases
ATGCCTGTTTTTTCGGATACTGAGAAGGATTTTTCCGTACGGTTGATGTACGGAATTATGCTCTCTTGCGGGGCGGTAAT
CATAAGCTATCGCACTCTGCATCCGATCTTTGCCGAAAAACCTAAAGGGGCTTACGCGGCAACGTATCTGATCCTCGCTG
CGATTATCACTTGTCACTTAATTGCAAAAAGCGGAAGAATTCTTTTAGCCTCTCATGTTCTAATCGGAATCGAATGGTTG
GCCGCATTCTATGGCATGATAGGGGAAGGTGCCACTCAAACGGTAGTTTTTCCATTTTGTTTGAGCTTAATTTTGATCTC
CGCACTTCTGTTGGGAATCAGGGCCGCTTATTTTTACACATTCCTAGCGATTGCAGTCGAGCTTACAAGTACCTATCTCA
TTCGGATCGAAGTGATTCGGCCCGTTAAAAGTACTGGACCTTGGTCCTCTTTGATCGGAGAAATTGGCTCTTTTATACTC
GTAGCAATTTTGATGAGATACACTTTGTTCGGATTTAGAATCGTAAAAACTGAATTGTCCGAAGTTCAACGTTATGCGAA
ATTAGGAGGATGGAGTCTGAATATACAAACTTTAGAACTTGTTCTCTCTAAAGAATACCAATACTTGCTCGGGTACGACG
ATGCGAAGGAATCCAAAACCTTACACTTGTCCACGTTCTTAAATACTTATGTCGTCGATGAAAAAGATAAGGCTCGTATC
CTCGACATTCTCGCAAAAAGCCGGACACAAAAAGAAATTACGGATTGCACCGTAGAGTTCGTTTATCAAATCCGAAGAAA
AGACGGACAACATCGATTCCTTGCTGGAAAGGGAAAATTTAGAGACTCGACCATCGGTATCGGAACGGCCCAAGACATCA
CGGAAAGACATCTAGCGGAAGAGGCTCTTCGTCCCGCTCAGGAAATTTATTCGAAGGTGTTTACCCTCAGTCCGATTGCG
ACAACGATTTCATCCGCGCATGATGGAAGATACGTCGAAGTCAACGATAGTTTTTTGAACTTGTTCGGATTTACGAGAGA
AGAAACAATCGGAAAGACGAGTATAGAATTGAACATTTGGCCCGATCCGGCTCAAAGAAAAGCATATTTCGATAAATTGT
CTCAAAAGAACGTACTCGTGGATGAGGAGTTGATTTTCCAAGGTAAAAACGGAAAAATTATTCACGCGGAATGTTATAGC
ACATTAGCTGAAATCAATGGAAAACTTTGTGCGATCAATCTTGTAAAAGATATTTCGGAAAAAAAAGAAGCGGAATCTCT
GAGAAAATTGAATAAGGAAATCTCCGATCAAAATAAATTGATCGAAAGGCAAAAGCAAGAACTCGAAGAAATTCTGAAGG
ATCTGAAAAAAACCCAAAATCAATTGATCATATCCGAAAAGATGGCCTCATTAGGTCAACTAGCGGCCGGAATTGCGCAT
GAAATCAACAATCCCATCGGAGTTGTGAAGTCGGCGAATGATTCCATACAGAATTATTTTAAAAGTTCCACAGATAGAAT
CATTCAGGTCTCCGAAACCCTCCAGGGATTAAATGTAGTGGTATTGAACGAACTCAGTACGTTCGTAAAAAAGGGAAAAA
AAAATCGGGAAATCCTATCTCCTAAGGAAATTCGCGAGAAAATAAAAAGGCTTGAAGTAAAATTGAAAGTAAAAGGATTC
GAAAATCCTCGTTCGGTTGCGCAAGACTTGGCGGAACTTGGTTTGGATTCTTCCGTGGAAGAATTTCCAAATCTGTTTAC
GCATAAGGACGGACAGGTACTATTACGTTTTGCGATCGCGGAAATTCAGGCCTTTCAAAATTCCAAGTTGATCGACATAT
CTGTGAATCGAACTTCCAAAGTCGTTTATGCTCTCAAAAAATTCACCCACTGGAGTTCGGAAGGAATCAAAATCCCGGTG
GTAATTTCGGAAAGTATAGAAACCGTTCTTACGATTTATCATAATCAACTGAAAATTGGAGTGGAAATCAATAGGGAATA
TAAAGATATTCCGACTATTCAAGCTTATGCGGATGATCTCATCCATCTATGGACGAATTTGATCTACAACGCCGTACAAG
CAATGTCCTTCAAAGGGATTTTAGGGATTCGAATTTCTCGTGTGGGAGAAAACGAAGTGGAAGTTGTCGTTTCGGATACG
GGACCCGGAATTTCCGCATTTGCGAAAGAAAGAATTTTCGAACCTTTTTTCACTACAAAGGCTCCAGGGGAAGGATCGGG
AATGGGTTTGGACATCGCAAAGAAAATCGTAGAAACGCATGAAGGAACAATCCGATTTGAAACTTCTCCCCAAGGAACTT
CTTTTTTTGTAAGACTTCCGATCTCCGAAACGAACACAAACTAA

Upstream 100 bases:

>100_bases
CCATCGCCAAAATCGGACTTTATAATCCAATTCTATAGGATAAAATGACACACCGATTTGATCAAGGTAGTCCCGATTGA
AAATACCAAGTTTTCTAAAA

Downstream 100 bases:

>100_bases
AATGGTGACCCTTCGGGAGCGAGATTTGAGTTTAGACAAAATGGCGCCCCTTCTGGGCTTGAAAAGCGTGGGTTAGATGC
GAGCATTTACCACAAAACAA

Product: sensor histidine kinase and response regulator of a two component complex

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 787; Mature: 786

Protein sequence:

>787_residues
MPVFSDTEKDFSVRLMYGIMLSCGAVIISYRTLHPIFAEKPKGAYAATYLILAAIITCHLIAKSGRILLASHVLIGIEWL
AAFYGMIGEGATQTVVFPFCLSLILISALLLGIRAAYFYTFLAIAVELTSTYLIRIEVIRPVKSTGPWSSLIGEIGSFIL
VAILMRYTLFGFRIVKTELSEVQRYAKLGGWSLNIQTLELVLSKEYQYLLGYDDAKESKTLHLSTFLNTYVVDEKDKARI
LDILAKSRTQKEITDCTVEFVYQIRRKDGQHRFLAGKGKFRDSTIGIGTAQDITERHLAEEALRPAQEIYSKVFTLSPIA
TTISSAHDGRYVEVNDSFLNLFGFTREETIGKTSIELNIWPDPAQRKAYFDKLSQKNVLVDEELIFQGKNGKIIHAECYS
TLAEINGKLCAINLVKDISEKKEAESLRKLNKEISDQNKLIERQKQELEEILKDLKKTQNQLIISEKMASLGQLAAGIAH
EINNPIGVVKSANDSIQNYFKSSTDRIIQVSETLQGLNVVVLNELSTFVKKGKKNREILSPKEIREKIKRLEVKLKVKGF
ENPRSVAQDLAELGLDSSVEEFPNLFTHKDGQVLLRFAIAEIQAFQNSKLIDISVNRTSKVVYALKKFTHWSSEGIKIPV
VISESIETVLTIYHNQLKIGVEINREYKDIPTIQAYADDLIHLWTNLIYNAVQAMSFKGILGIRISRVGENEVEVVVSDT
GPGISAFAKERIFEPFFTTKAPGEGSGMGLDIAKKIVETHEGTIRFETSPQGTSFFVRLPISETNTN

Sequences:

>Translated_787_residues
MPVFSDTEKDFSVRLMYGIMLSCGAVIISYRTLHPIFAEKPKGAYAATYLILAAIITCHLIAKSGRILLASHVLIGIEWL
AAFYGMIGEGATQTVVFPFCLSLILISALLLGIRAAYFYTFLAIAVELTSTYLIRIEVIRPVKSTGPWSSLIGEIGSFIL
VAILMRYTLFGFRIVKTELSEVQRYAKLGGWSLNIQTLELVLSKEYQYLLGYDDAKESKTLHLSTFLNTYVVDEKDKARI
LDILAKSRTQKEITDCTVEFVYQIRRKDGQHRFLAGKGKFRDSTIGIGTAQDITERHLAEEALRPAQEIYSKVFTLSPIA
TTISSAHDGRYVEVNDSFLNLFGFTREETIGKTSIELNIWPDPAQRKAYFDKLSQKNVLVDEELIFQGKNGKIIHAECYS
TLAEINGKLCAINLVKDISEKKEAESLRKLNKEISDQNKLIERQKQELEEILKDLKKTQNQLIISEKMASLGQLAAGIAH
EINNPIGVVKSANDSIQNYFKSSTDRIIQVSETLQGLNVVVLNELSTFVKKGKKNREILSPKEIREKIKRLEVKLKVKGF
ENPRSVAQDLAELGLDSSVEEFPNLFTHKDGQVLLRFAIAEIQAFQNSKLIDISVNRTSKVVYALKKFTHWSSEGIKIPV
VISESIETVLTIYHNQLKIGVEINREYKDIPTIQAYADDLIHLWTNLIYNAVQAMSFKGILGIRISRVGENEVEVVVSDT
GPGISAFAKERIFEPFFTTKAPGEGSGMGLDIAKKIVETHEGTIRFETSPQGTSFFVRLPISETNTN
>Mature_786_residues
PVFSDTEKDFSVRLMYGIMLSCGAVIISYRTLHPIFAEKPKGAYAATYLILAAIITCHLIAKSGRILLASHVLIGIEWLA
AFYGMIGEGATQTVVFPFCLSLILISALLLGIRAAYFYTFLAIAVELTSTYLIRIEVIRPVKSTGPWSSLIGEIGSFILV
AILMRYTLFGFRIVKTELSEVQRYAKLGGWSLNIQTLELVLSKEYQYLLGYDDAKESKTLHLSTFLNTYVVDEKDKARIL
DILAKSRTQKEITDCTVEFVYQIRRKDGQHRFLAGKGKFRDSTIGIGTAQDITERHLAEEALRPAQEIYSKVFTLSPIAT
TISSAHDGRYVEVNDSFLNLFGFTREETIGKTSIELNIWPDPAQRKAYFDKLSQKNVLVDEELIFQGKNGKIIHAECYST
LAEINGKLCAINLVKDISEKKEAESLRKLNKEISDQNKLIERQKQELEEILKDLKKTQNQLIISEKMASLGQLAAGIAHE
INNPIGVVKSANDSIQNYFKSSTDRIIQVSETLQGLNVVVLNELSTFVKKGKKNREILSPKEIREKIKRLEVKLKVKGFE
NPRSVAQDLAELGLDSSVEEFPNLFTHKDGQVLLRFAIAEIQAFQNSKLIDISVNRTSKVVYALKKFTHWSSEGIKIPVV
ISESIETVLTIYHNQLKIGVEINREYKDIPTIQAYADDLIHLWTNLIYNAVQAMSFKGILGIRISRVGENEVEVVVSDTG
PGISAFAKERIFEPFFTTKAPGEGSGMGLDIAKKIVETHEGTIRFETSPQGTSFFVRLPISETNTN

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=197, Percent_Identity=32.48730964467, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788549, Length=177, Percent_Identity=28.2485875706215, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI1790300, Length=199, Percent_Identity=27.1356783919598, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 88283; Mature: 88152

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVFSDTEKDFSVRLMYGIMLSCGAVIISYRTLHPIFAEKPKGAYAATYLILAAIITCHL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHH
IAKSGRILLASHVLIGIEWLAAFYGMIGEGATQTVVFPFCLSLILISALLLGIRAAYFYT
HHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
FLAIAVELTSTYLIRIEVIRPVKSTGPWSSLIGEIGSFILVAILMRYTLFGFRIVKTELS
HHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVQRYAKLGGWSLNIQTLELVLSKEYQYLLGYDDAKESKTLHLSTFLNTYVVDEKDKARI
HHHHHHHHCCCEEEHHHHHHHHHHCCCEEECCCCCCCCCEEEHHHHHHHHEECCCCHHHH
LDILAKSRTQKEITDCTVEFVYQIRRKDGQHRFLAGKGKFRDSTIGIGTAQDITERHLAE
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
EALRPAQEIYSKVFTLSPIATTISSAHDGRYVEVNDSFLNLFGFTREETIGKTSIELNIW
HHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEECHHHHHHHCCCHHHCCCCEEEEEEEC
PDPAQRKAYFDKLSQKNVLVDEELIFQGKNGKIIHAECYSTLAEINGKLCAINLVKDISE
CCCHHHHHHHHHHCCCCCEECHHHEEECCCCCEEEHHHHHHHHHCCCCEEEEHHHHHHHH
KKEAESLRKLNKEISDQNKLIERQKQELEEILKDLKKTQNQLIISEKMASLGQLAAGIAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EINNPIGVVKSANDSIQNYFKSSTDRIIQVSETLQGLNVVVLNELSTFVKKGKKNREILS
HCCCCCEEEECCCHHHHHHHHCCHHHEEEHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCC
PKEIREKIKRLEVKLKVKGFENPRSVAQDLAELGLDSSVEEFPNLFTHKDGQVLLRFAIA
HHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHH
EIQAFQNSKLIDISVNRTSKVVYALKKFTHWSSEGIKIPVVISESIETVLTIYHNQLKIG
HHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHCCEEEE
VEINREYKDIPTIQAYADDLIHLWTNLIYNAVQAMSFKGILGIRISRVGENEVEVVVSDT
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECC
GPGISAFAKERIFEPFFTTKAPGEGSGMGLDIAKKIVETHEGTIRFETSPQGTSFFVRLP
CCCHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEEE
ISETNTN
CCCCCCC
>Mature Secondary Structure 
PVFSDTEKDFSVRLMYGIMLSCGAVIISYRTLHPIFAEKPKGAYAATYLILAAIITCHL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHH
IAKSGRILLASHVLIGIEWLAAFYGMIGEGATQTVVFPFCLSLILISALLLGIRAAYFYT
HHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
FLAIAVELTSTYLIRIEVIRPVKSTGPWSSLIGEIGSFILVAILMRYTLFGFRIVKTELS
HHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVQRYAKLGGWSLNIQTLELVLSKEYQYLLGYDDAKESKTLHLSTFLNTYVVDEKDKARI
HHHHHHHHCCCEEEHHHHHHHHHHCCCEEECCCCCCCCCEEEHHHHHHHHEECCCCHHHH
LDILAKSRTQKEITDCTVEFVYQIRRKDGQHRFLAGKGKFRDSTIGIGTAQDITERHLAE
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
EALRPAQEIYSKVFTLSPIATTISSAHDGRYVEVNDSFLNLFGFTREETIGKTSIELNIW
HHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEECHHHHHHHCCCHHHCCCCEEEEEEEC
PDPAQRKAYFDKLSQKNVLVDEELIFQGKNGKIIHAECYSTLAEINGKLCAINLVKDISE
CCCHHHHHHHHHHCCCCCEECHHHEEECCCCCEEEHHHHHHHHHCCCCEEEEHHHHHHHH
KKEAESLRKLNKEISDQNKLIERQKQELEEILKDLKKTQNQLIISEKMASLGQLAAGIAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EINNPIGVVKSANDSIQNYFKSSTDRIIQVSETLQGLNVVVLNELSTFVKKGKKNREILS
HCCCCCEEEECCCHHHHHHHHCCHHHEEEHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCC
PKEIREKIKRLEVKLKVKGFENPRSVAQDLAELGLDSSVEEFPNLFTHKDGQVLLRFAIA
HHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHH
EIQAFQNSKLIDISVNRTSKVVYALKKFTHWSSEGIKIPVVISESIETVLTIYHNQLKIG
HHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHCCEEEE
VEINREYKDIPTIQAYADDLIHLWTNLIYNAVQAMSFKGILGIRISRVGENEVEVVVSDT
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECC
GPGISAFAKERIFEPFFTTKAPGEGSGMGLDIAKKIVETHEGTIRFETSPQGTSFFVRLP
CCCHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEEE
ISETNTN
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]