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The map label for this gene is miaB-3 [H]

Identifier: 116327128

GI number: 116327128

Start: 360355

End: 361686

Strand: Direct

Name: miaB-3 [H]

Synonym: LBL_0304

Alternate gene names: 116327128

Gene position: 360355-361686 (Clockwise)

Preceding gene: 116327127

Following gene: 116327129

Centisome position: 9.97

GC content: 40.69

Gene sequence:

>1332_bases
ATGCCCTCGTCACCAATAGCTGAAAGAACGGTTCTTTTCAATACTTTAGGATGCAGGCTTAACTTTTTCGAGTCGGACGG
TTTATTTTCGTCCTTGAGCAAACACGGATTTCGTTCCGTAGAAGTAGGGGAACATCCTGAAGTAGTCATCATCAATACTT
GCACCGTGACCAATAAGGCTGATTCTAAAAATCGAAATACAATTCGAAATGCGATCAAAAAGTTTCCGGGTTCTCAAATC
TGGGTTACCGGATGTTATGCCGAAACGGATCGTGAGTCAATCGAAGCGATTCCAGGTGTGGCCGGAGTTGTCGGAAATAC
GGAGAAATCAAAACTTCCCGTCATGATCTTGGAAAAGAAAGGTTTGATCGATTCGAATCAGCTGATTCAATTTTCTTACG
ATCGTTTTTCTTATTCGGACGTTTTGCCGAACGGTCATACGCGCGCTTATTTGAAAATCCAAGATGGGTGTAATCGTAGA
TGTTCGTATTGTAAAATCCCGCAGGCGCGCGGCCTTGGAGTTAGCAGAAAATATCAAGACGTATTGGATCAAGTTCATTT
TTTACAAGATCATGGGGTTGGAGAGATCGTTCTGACCGGTGTAAATTTAGGCTGGTATCGGGACGGTGAAAATAAAAAAG
CCTTCAACAAAATCCTGGCGGATATTTTAAAGATTTTAGAATATTCGAGAATTCGAATTTCTTCCATAGAGCCCCCCGAT
GTCGGAAACGAGCTTGTGGAGTTGATGACTCATCCTCGTTTTACTCCGTTTCTTCATGTTCCATTACAGAGTGGAAATGC
CGAAATTCTAAAAAAGATGAAACGCACGTACACGCCGGAAACTTTTCGAAAACGTGTGGAAATTGCCAAAGAAAAAATTC
CAAATTTGTTTTTAGGAACGGACATAATCGTAGGTTTTCCCGGTGAGACCGAGGAAATGTTTTTAGATTCGGTAAAAATG
ATTCAAGATCTAGGTTTTGCTAAGATCCACGCATTCCCTTTTTCCGTCAGAAGAAATACATTAGCCGAAACATTTCCGGA
TTCGGTTAGTAAAGAAATCAAAAAGGGAAGGGTTCATTCGCTGAATTCTTTGTCCAGAAAACTTCATGAAAATTATTCCT
TATCCGTCATCGGACAAGTACGGGAAGCGATTTTAGAACAAGGCGGAACGGCCGTAACCGACAACTATCTCAAAGTGAAA
CTTTCCGATCTGGAGTTAAAAAATTTGAAAATCGGTCAGTTCTTAAATGTGGAACTTTTAGGATACGAAGCGGGTATCGA
CAAAGAAGGAAATTTTTTAGGTAAGGTTTTCAAGATAAAATCGTCTGAGTAA

Upstream 100 bases:

>100_bases
TTTTTAAATTCCGCAAAAGAACAAATCGAATCCGCACTGAAACTCGAGCCTGAAAATCCCCACTTTAAAGATAATCTAAA
AAGAATTGAAGCTAAACTTA

Downstream 100 bases:

>100_bases
AAAAGTAATCGATTGAAACGTTCAACAAATCGAATATTTCGTTTTTTAACGTGAGTTCGGCCTAACAAATGCGAAAGTGA
TTATTATGCTTGCGATCTGT

Product: 2-methylthioadenine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MPSSPIAERTVLFNTLGCRLNFFESDGLFSSLSKHGFRSVEVGEHPEVVIINTCTVTNKADSKNRNTIRNAIKKFPGSQI
WVTGCYAETDRESIEAIPGVAGVVGNTEKSKLPVMILEKKGLIDSNQLIQFSYDRFSYSDVLPNGHTRAYLKIQDGCNRR
CSYCKIPQARGLGVSRKYQDVLDQVHFLQDHGVGEIVLTGVNLGWYRDGENKKAFNKILADILKILEYSRIRISSIEPPD
VGNELVELMTHPRFTPFLHVPLQSGNAEILKKMKRTYTPETFRKRVEIAKEKIPNLFLGTDIIVGFPGETEEMFLDSVKM
IQDLGFAKIHAFPFSVRRNTLAETFPDSVSKEIKKGRVHSLNSLSRKLHENYSLSVIGQVREAILEQGGTAVTDNYLKVK
LSDLELKNLKIGQFLNVELLGYEAGIDKEGNFLGKVFKIKSSE

Sequences:

>Translated_443_residues
MPSSPIAERTVLFNTLGCRLNFFESDGLFSSLSKHGFRSVEVGEHPEVVIINTCTVTNKADSKNRNTIRNAIKKFPGSQI
WVTGCYAETDRESIEAIPGVAGVVGNTEKSKLPVMILEKKGLIDSNQLIQFSYDRFSYSDVLPNGHTRAYLKIQDGCNRR
CSYCKIPQARGLGVSRKYQDVLDQVHFLQDHGVGEIVLTGVNLGWYRDGENKKAFNKILADILKILEYSRIRISSIEPPD
VGNELVELMTHPRFTPFLHVPLQSGNAEILKKMKRTYTPETFRKRVEIAKEKIPNLFLGTDIIVGFPGETEEMFLDSVKM
IQDLGFAKIHAFPFSVRRNTLAETFPDSVSKEIKKGRVHSLNSLSRKLHENYSLSVIGQVREAILEQGGTAVTDNYLKVK
LSDLELKNLKIGQFLNVELLGYEAGIDKEGNFLGKVFKIKSSE
>Mature_442_residues
PSSPIAERTVLFNTLGCRLNFFESDGLFSSLSKHGFRSVEVGEHPEVVIINTCTVTNKADSKNRNTIRNAIKKFPGSQIW
VTGCYAETDRESIEAIPGVAGVVGNTEKSKLPVMILEKKGLIDSNQLIQFSYDRFSYSDVLPNGHTRAYLKIQDGCNRRC
SYCKIPQARGLGVSRKYQDVLDQVHFLQDHGVGEIVLTGVNLGWYRDGENKKAFNKILADILKILEYSRIRISSIEPPDV
GNELVELMTHPRFTPFLHVPLQSGNAEILKKMKRTYTPETFRKRVEIAKEKIPNLFLGTDIIVGFPGETEEMFLDSVKMI
QDLGFAKIHAFPFSVRRNTLAETFPDSVSKEIKKGRVHSLNSLSRKLHENYSLSVIGQVREAILEQGGTAVTDNYLKVKL
SDLELKNLKIGQFLNVELLGYEAGIDKEGNFLGKVFKIKSSE

Specific function: Unknown

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=449, Percent_Identity=26.9487750556793, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI28872782, Length=449, Percent_Identity=26.9487750556793, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI93277076, Length=376, Percent_Identity=28.7234042553192, Blast_Score=145, Evalue=1e-34,
Organism=Escherichia coli, GI1786882, Length=427, Percent_Identity=30.9133489461358, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1787057, Length=366, Percent_Identity=27.5956284153005, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553146, Length=471, Percent_Identity=26.963906581741, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI71996771, Length=285, Percent_Identity=28.4210526315789, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI21356207, Length=427, Percent_Identity=27.6346604215457, Blast_Score=174, Evalue=9e-44,
Organism=Drosophila melanogaster, GI19922432, Length=369, Percent_Identity=28.1842818428184, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006467
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49778; Mature: 49647

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSPIAERTVLFNTLGCRLNFFESDGLFSSLSKHGFRSVEVGEHPEVVIINTCTVTNKA
CCCCCCHHHHHHEEECCCEEEEECCCCHHHHHHHCCCCEEECCCCCCEEEEEEEEECCCC
DSKNRNTIRNAIKKFPGSQIWVTGCYAETDRESIEAIPGVAGVVGNTEKSKLPVMILEKK
CCCCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHCCCCHHHCCCCCCCCCCEEEEECC
GLIDSNQLIQFSYDRFSYSDVLPNGHTRAYLKIQDGCNRRCSYCKIPQARGLGVSRKYQD
CCCCCCCEEEEEECCCCHHHCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCCCHHHHH
VLDQVHFLQDHGVGEIVLTGVNLGWYRDGENKKAFNKILADILKILEYSRIRISSIEPPD
HHHHHHHHHHCCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCC
VGNELVELMTHPRFTPFLHVPLQSGNAEILKKMKRTYTPETFRKRVEIAKEKIPNLFLGT
CHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECC
DIIVGFPGETEEMFLDSVKMIQDLGFAKIHAFPFSVRRNTLAETFPDSVSKEIKKGRVHS
EEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHCCCCHH
LNSLSRKLHENYSLSVIGQVREAILEQGGTAVTDNYLKVKLSDLELKNLKIGQFLNVELL
HHHHHHHHHCCCCEEHHHHHHHHHHHCCCCEEECCEEEEEEECCEECCEECCCEEEEEEE
GYEAGIDKEGNFLGKVFKIKSSE
EEECCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
PSSPIAERTVLFNTLGCRLNFFESDGLFSSLSKHGFRSVEVGEHPEVVIINTCTVTNKA
CCCCCHHHHHHEEECCCEEEEECCCCHHHHHHHCCCCEEECCCCCCEEEEEEEEECCCC
DSKNRNTIRNAIKKFPGSQIWVTGCYAETDRESIEAIPGVAGVVGNTEKSKLPVMILEKK
CCCCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHCCCCHHHCCCCCCCCCCEEEEECC
GLIDSNQLIQFSYDRFSYSDVLPNGHTRAYLKIQDGCNRRCSYCKIPQARGLGVSRKYQD
CCCCCCCEEEEEECCCCHHHCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCCCHHHHH
VLDQVHFLQDHGVGEIVLTGVNLGWYRDGENKKAFNKILADILKILEYSRIRISSIEPPD
HHHHHHHHHHCCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCC
VGNELVELMTHPRFTPFLHVPLQSGNAEILKKMKRTYTPETFRKRVEIAKEKIPNLFLGT
CHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECC
DIIVGFPGETEEMFLDSVKMIQDLGFAKIHAFPFSVRRNTLAETFPDSVSKEIKKGRVHS
EEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHCCCCHH
LNSLSRKLHENYSLSVIGQVREAILEQGGTAVTDNYLKVKLSDLELKNLKIGQFLNVELL
HHHHHHHHHCCCCEEHHHHHHHHHHHCCCCEEECCEEEEEEECCEECCEECCCEEEEEEE
GYEAGIDKEGNFLGKVFKIKSSE
EEECCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]