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The map label for this gene is cyoE

Identifier: 116327097

GI number: 116327097

Start: 323158

End: 324027

Strand: Reverse

Name: cyoE

Synonym: LBL_0272

Alternate gene names: 116327097

Gene position: 324027-323158 (Counterclockwise)

Preceding gene: 116327098

Following gene: 116327096

Centisome position: 8.96

GC content: 41.26

Gene sequence:

>870_bases
ATGGCAAGTTCCACTTTCTTTTCCGATTGGAATCAATTGATCAAACCGCGGGTAACGTCCCTGGTACTAGCTACCATCAT
TCCAGGCTTGTATCTCGCAAGTGAACAACCACCTTCTGTATTTCTGATCACAGTTACTTTATTCGGAACGTTTTTGATGT
CCTCCGCCTCGTTTATTTTCAATCAGGTAATCGAAAGGGATCGAGACGCAAAAATGAAACGAACCTCCAACCGTCCCATT
CCTTCGGAAAGAATCAGCATTCCACAGGCAACGTTAGTCGGCATTTCTATGATGGGATTATCCTTTTACATGTTGACCGT
TTATGTAAATCTTTTGACGGCCTTGTGCGCTCTCACCGCTTTGATTTCCTACGTTTTTCTTTATACGATTTTTTTAAAAC
CTAGAACTACCCAGAATATCGTTATCGGCGGAGTGGCTGGATGTGTAGGACCTTTAATCGGCTATGCAGCTATCGGAAAC
TCACTCCCGATTCAAGCATGGATTTTGTTCACAATGATTTTCTTATGGACTCCGGCACACTTTTGGGCCTTGGCAATCTT
TCTCAAGGAAGATTATTCCGACGCGAATTTCCCGATGTTGCCCGTCGTCAAAGGAATTAACCAAACGACGAAATCCATAT
TCTTTTATACGATTCTCTATTCTCTTTCTTGTGTTAGCTTTTACTTTTTAGAACCTTCGATGGGCCTCTTATATCTCGTT
ATTGTATTACTCGTTTGTATCTGGATGGGGATTCTCTCCTATCGATTGATCCAAAACCCGGAGCGTCAAGCCGCGAGAAA
ATTTTTCCTCTTCTCAATCTTTCATCTTTTTCTAATAAATATAACAATCGTAGTCGATCATATGATTTAG

Upstream 100 bases:

>100_bases
TCTTTTACTCATGTTTTCTTATCTTTCCCTGATTTCCAGAGCCATTGTACTGACTTCGGAAAACGAACGGAGGCCTGAAA
AATAACTTCAGGATACTATA

Downstream 100 bases:

>100_bases
AGCCTGTTCTCAAAATCTTTAAACTTATCACAATACCTCAGAATGTAGGAACTTTTTAGATGTTAGTGGATAGATTTTAG
AACTTCACATACAATCAAGT

Product: polyprenyltransferase

Products: NA

Alternate protein names: Heme B farnesyltransferase; Heme O synthase

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MASSTFFSDWNQLIKPRVTSLVLATIIPGLYLASEQPPSVFLITVTLFGTFLMSSASFIFNQVIERDRDAKMKRTSNRPI
PSERISIPQATLVGISMMGLSFYMLTVYVNLLTALCALTALISYVFLYTIFLKPRTTQNIVIGGVAGCVGPLIGYAAIGN
SLPIQAWILFTMIFLWTPAHFWALAIFLKEDYSDANFPMLPVVKGINQTTKSIFFYTILYSLSCVSFYFLEPSMGLLYLV
IVLLVCIWMGILSYRLIQNPERQAARKFFLFSIFHLFLINITIVVDHMI

Sequences:

>Translated_289_residues
MASSTFFSDWNQLIKPRVTSLVLATIIPGLYLASEQPPSVFLITVTLFGTFLMSSASFIFNQVIERDRDAKMKRTSNRPI
PSERISIPQATLVGISMMGLSFYMLTVYVNLLTALCALTALISYVFLYTIFLKPRTTQNIVIGGVAGCVGPLIGYAAIGN
SLPIQAWILFTMIFLWTPAHFWALAIFLKEDYSDANFPMLPVVKGINQTTKSIFFYTILYSLSCVSFYFLEPSMGLLYLV
IVLLVCIWMGILSYRLIQNPERQAARKFFLFSIFHLFLINITIVVDHMI
>Mature_288_residues
ASSTFFSDWNQLIKPRVTSLVLATIIPGLYLASEQPPSVFLITVTLFGTFLMSSASFIFNQVIERDRDAKMKRTSNRPIP
SERISIPQATLVGISMMGLSFYMLTVYVNLLTALCALTALISYVFLYTIFLKPRTTQNIVIGGVAGCVGPLIGYAAIGNS
LPIQAWILFTMIFLWTPAHFWALAIFLKEDYSDANFPMLPVVKGINQTTKSIFFYTILYSLSCVSFYFLEPSMGLLYLVI
VLLVCIWMGILSYRLIQNPERQAARKFFLFSIFHLFLINITIVVDHMI

Specific function: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group

COG id: COG0109

COG function: function code O; Polyprenyltransferase (cytochrome oxidase assembly factor)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily

Homologues:

Organism=Homo sapiens, GI17921982, Length=195, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1786631, Length=287, Percent_Identity=32.7526132404181, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1790473, Length=256, Percent_Identity=25, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71998235, Length=277, Percent_Identity=30.6859205776173, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6325085, Length=284, Percent_Identity=26.4084507042254, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19921064, Length=277, Percent_Identity=30.3249097472924, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COXX_LEPBJ (Q04PG4)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801964.1
- STRING:   Q04PG4
- GeneID:   4410184
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2799
- eggNOG:   COG0109
- HOGENOM:   HBG656003
- OMA:   LLKPRIM
- PhylomeDB:   Q04PG4
- ProtClustDB:   CLSK573404
- BioCyc:   LBOR355277:LBJ_2799-MONOMER
- HAMAP:   MF_00154
- InterPro:   IPR006369
- InterPro:   IPR000537
- PANTHER:   PTHR11048:SF3
- TIGRFAMs:   TIGR01473

Pfam domain/function: PF01040 UbiA

EC number: 2.5.1.-

Molecular weight: Translated: 32618; Mature: 32487

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS00943 UBIA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc218f94)-; HASH(0xe334200)-; HASH(0xea324b4)-; HASH(0xea2d35c)-; HASH(0xea2a37c)-; HASH(0xea34a70)-; HASH(0xda73910)-; HASH(0xe2a9bb0)-; HASH(0xe6b3ea0)-;

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSTFFSDWNQLIKPRVTSLVLATIIPGLYLASEQPPSVFLITVTLFGTFLMSSASFIF
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
NQVIERDRDAKMKRTSNRPIPSERISIPQATLVGISMMGLSFYMLTVYVNLLTALCALTA
HHHHHCCHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LISYVFLYTIFLKPRTTQNIVIGGVAGCVGPLIGYAAIGNSLPIQAWILFTMIFLWTPAH
HHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHH
FWALAIFLKEDYSDANFPMLPVVKGINQTTKSIFFYTILYSLSCVSFYFLEPSMGLLYLV
HHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IVLLVCIWMGILSYRLIQNPERQAARKFFLFSIFHLFLINITIVVDHMI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
ASSTFFSDWNQLIKPRVTSLVLATIIPGLYLASEQPPSVFLITVTLFGTFLMSSASFIF
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
NQVIERDRDAKMKRTSNRPIPSERISIPQATLVGISMMGLSFYMLTVYVNLLTALCALTA
HHHHHCCHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LISYVFLYTIFLKPRTTQNIVIGGVAGCVGPLIGYAAIGNSLPIQAWILFTMIFLWTPAH
HHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHH
FWALAIFLKEDYSDANFPMLPVVKGINQTTKSIFFYTILYSLSCVSFYFLEPSMGLLYLV
HHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IVLLVCIWMGILSYRLIQNPERQAARKFFLFSIFHLFLINITIVVDHMI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA