Click here to switch to the map view.

The map label for this gene is leuB-2 [H]

Identifier: 116327096

GI number: 116327096

Start: 321399

End: 322865

Strand: Reverse

Name: leuB-2 [H]

Synonym: LBL_0271

Alternate gene names: 116327096

Gene position: 322865-321399 (Counterclockwise)

Preceding gene: 116327097

Following gene: 116327095

Centisome position: 8.93

GC content: 42.13

Gene sequence:

>1467_bases
ATGTCCGAAAAAACTAAAATTGCAATCGCACACGGAGACGGAATCGGTCCTGAAATTATGGATGCAACTCTCAAAATTCT
CAATGCGGCCGGAGCAAAAATCGATCCGATCGAAATAGAAATTGGAGAAAGAGTTTATAAGGACGGACATTCTTCCGGAA
TTAAACCCGAAGCTTGGGACCTTCTCAGACAAACTAAAGTATTTTTGAAGGCCCCTATTACTACTCCTCAAGGTGGTGGT
TACAAAAGTTTGAACGTAACCGTAAGAACCACTTTGGGACTTTTTGCCAACGTCAGACCCTGTTTTTCTCTATATCCGTA
TGTGGAAACCAAACACCCTTCTCTCGACATTGTCATCATTCGTGAAAACGAGGAAGACCTTTATACAGGAATCGAACATA
GACAAACCAACGATACGGTTCAATGTCTCAAATTGATTTCCCGTCCAGGCTCGGAAAAAATCATTCGTTACGCCTTCGAA
TACGCCAGAGCCTACGGAAGAAAAAAAGTAACAGCAATGGTAAAGGACAATATCATGAAACAGACTGACGGTTTATTTCA
TGATATCTTTAAGGAAATTGCACAAGGATATCCCGAACTCGAAGCAAATTCACAGATCATCGACATCGGCGCCGCCAATC
TTGCGGATAGACCACAGAACTTTGATGTGGTCGTAACTTTGAACTTGTACGGAGACATCATCTCCGATATCGTTGCACAA
ATTGCGGGGTCGGTTGGAATGGCTGGATCTTCCAATATCGGTGAAATTGTTTCTATGTTCGAAGCGATTCACGGTTCCGC
CCCCGATATTGCAGGGAAAAATCTCGCAAATCCATCCGGCTTACTCAACGCCGCAGTGATGATGCTTGTTCATATAGGGC
AACCCGATATCGCGGCAAAAATCAACAACGCTTGGCTCTTAACGGTCGAAGAGGGAATTCACACCGGAGATATTTTTAAA
CCTGGCGTAAGCAGAATCAAAGTCGGAACCAAAGAATTTGCCGACGCGGTAATCGGAAACCTGGGTCATCTTCCAGAAAA
ATTCAAACCCGTTTCTTTCGGCAAGGCGAAGAAAATTATAATTCCTGAATATAAGAGAATTATTCAAAAGAAAGAACTGG
TCGGAGTTGATATTTTTCTAGATTGGATCGGCAACGACCCGAACGAACTTGCAAACAAACTTAAATCTCTTTCTAACGAT
CTAATGTTAAAAATCATTACCAATCGAGGAGTAAAAGTTTATCCCGAAGGACAACCTGAAACCTTTTTGATCGACCATTG
GAGATGTAGATTCGTGAGCAAGAACGCCCTGATTAAACAAGAGGATCCTTCGTATCATCCGATTTCTCATAAACAAGTTG
CGGAACTTCTCTTAAAACTGGACTCAGTCGGTTTTGATACGATCAAAACGGAAAATCTATATTATTTTAACGGAAAAAGG
GCCTTTTCCTTAGGACAGGGAGAGTGA

Upstream 100 bases:

>100_bases
TGCCGCATATAATTTTTACGATAGGTGATTATCGATGAGAATTCGATAAGAATTGACCTATTTACCGTCCTCGTTCTAAT
AGAAGTATTAGGAGACGAGA

Downstream 100 bases:

>100_bases
ATAGTCGTCCCTGAAAAAGTATAGAGAATTCAATTTAAAACGAGAAAAGATGAACTTGAAGATCTCTTTTCCTTTGGAAA
GCAGTCTATTTACACTCTAT

Product: isocitrate dehydrogenase

Products: NA

Alternate protein names: IDH; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSEKTKIAIAHGDGIGPEIMDATLKILNAAGAKIDPIEIEIGERVYKDGHSSGIKPEAWDLLRQTKVFLKAPITTPQGGG
YKSLNVTVRTTLGLFANVRPCFSLYPYVETKHPSLDIVIIRENEEDLYTGIEHRQTNDTVQCLKLISRPGSEKIIRYAFE
YARAYGRKKVTAMVKDNIMKQTDGLFHDIFKEIAQGYPELEANSQIIDIGAANLADRPQNFDVVVTLNLYGDIISDIVAQ
IAGSVGMAGSSNIGEIVSMFEAIHGSAPDIAGKNLANPSGLLNAAVMMLVHIGQPDIAAKINNAWLLTVEEGIHTGDIFK
PGVSRIKVGTKEFADAVIGNLGHLPEKFKPVSFGKAKKIIIPEYKRIIQKKELVGVDIFLDWIGNDPNELANKLKSLSND
LMLKIITNRGVKVYPEGQPETFLIDHWRCRFVSKNALIKQEDPSYHPISHKQVAELLLKLDSVGFDTIKTENLYYFNGKR
AFSLGQGE

Sequences:

>Translated_488_residues
MSEKTKIAIAHGDGIGPEIMDATLKILNAAGAKIDPIEIEIGERVYKDGHSSGIKPEAWDLLRQTKVFLKAPITTPQGGG
YKSLNVTVRTTLGLFANVRPCFSLYPYVETKHPSLDIVIIRENEEDLYTGIEHRQTNDTVQCLKLISRPGSEKIIRYAFE
YARAYGRKKVTAMVKDNIMKQTDGLFHDIFKEIAQGYPELEANSQIIDIGAANLADRPQNFDVVVTLNLYGDIISDIVAQ
IAGSVGMAGSSNIGEIVSMFEAIHGSAPDIAGKNLANPSGLLNAAVMMLVHIGQPDIAAKINNAWLLTVEEGIHTGDIFK
PGVSRIKVGTKEFADAVIGNLGHLPEKFKPVSFGKAKKIIIPEYKRIIQKKELVGVDIFLDWIGNDPNELANKLKSLSND
LMLKIITNRGVKVYPEGQPETFLIDHWRCRFVSKNALIKQEDPSYHPISHKQVAELLLKLDSVGFDTIKTENLYYFNGKR
AFSLGQGE
>Mature_487_residues
SEKTKIAIAHGDGIGPEIMDATLKILNAAGAKIDPIEIEIGERVYKDGHSSGIKPEAWDLLRQTKVFLKAPITTPQGGGY
KSLNVTVRTTLGLFANVRPCFSLYPYVETKHPSLDIVIIRENEEDLYTGIEHRQTNDTVQCLKLISRPGSEKIIRYAFEY
ARAYGRKKVTAMVKDNIMKQTDGLFHDIFKEIAQGYPELEANSQIIDIGAANLADRPQNFDVVVTLNLYGDIISDIVAQI
AGSVGMAGSSNIGEIVSMFEAIHGSAPDIAGKNLANPSGLLNAAVMMLVHIGQPDIAAKINNAWLLTVEEGIHTGDIFKP
GVSRIKVGTKEFADAVIGNLGHLPEKFKPVSFGKAKKIIIPEYKRIIQKKELVGVDIFLDWIGNDPNELANKLKSLSNDL
MLKIITNRGVKVYPEGQPETFLIDHWRCRFVSKNALIKQEDPSYHPISHKQVAELLLKLDSVGFDTIKTENLYYFNGKRA
FSLGQGE

Specific function: Unknown

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=311, Percent_Identity=44.0514469453376, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI4758582, Length=317, Percent_Identity=38.1703470031546, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI28178838, Length=302, Percent_Identity=38.0794701986755, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI28178821, Length=285, Percent_Identity=36.8421052631579, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI28178816, Length=285, Percent_Identity=36.8421052631579, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI28178819, Length=155, Percent_Identity=40, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1787381, Length=392, Percent_Identity=34.1836734693878, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788101, Length=362, Percent_Identity=32.0441988950276, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI87081683, Length=366, Percent_Identity=32.5136612021858, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI71986051, Length=322, Percent_Identity=43.167701863354, Blast_Score=247, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI25144293, Length=323, Percent_Identity=39.6284829721362, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17505779, Length=313, Percent_Identity=37.3801916932907, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI17550882, Length=349, Percent_Identity=34.3839541547278, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6324709, Length=345, Percent_Identity=42.8985507246377, Blast_Score=250, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6324291, Length=309, Percent_Identity=38.5113268608414, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6322097, Length=355, Percent_Identity=33.8028169014084, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319830, Length=363, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24643268, Length=318, Percent_Identity=43.3962264150943, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24643270, Length=318, Percent_Identity=43.3962264150943, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24661184, Length=342, Percent_Identity=41.5204678362573, Blast_Score=245, Evalue=6e-65,
Organism=Drosophila melanogaster, GI161078637, Length=312, Percent_Identity=36.5384615384615, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI161078635, Length=312, Percent_Identity=36.5384615384615, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI161078639, Length=312, Percent_Identity=36.5384615384615, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI161078633, Length=312, Percent_Identity=36.5384615384615, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24650122, Length=312, Percent_Identity=36.5384615384615, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI281362242, Length=347, Percent_Identity=32.8530259365994, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24648872, Length=347, Percent_Identity=32.8530259365994, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI20130355, Length=338, Percent_Identity=28.698224852071, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI45550436, Length=214, Percent_Identity=24.2990654205607, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 8180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR014273 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.42 [H]

Molecular weight: Translated: 53880; Mature: 53749

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKTKIAIAHGDGIGPEIMDATLKILNAAGAKIDPIEIEIGERVYKDGHSSGIKPEAWD
CCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHCCCCCCCCHHHHH
LLRQTKVFLKAPITTPQGGGYKSLNVTVRTTLGLFANVRPCFSLYPYVETKHPSLDIVII
HHHHHHHHEECCCCCCCCCCEEEEEEEEEEHHHHHHCCCHHHHHCCCEECCCCCEEEEEE
RENEEDLYTGIEHRQTNDTVQCLKLISRPGSEKIIRYAFEYARAYGRKKVTAMVKDNIMK
ECCCHHHHHCCHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
QTDGLFHDIFKEIAQGYPELEANSQIIDIGAANLADRPQNFDVVVTLNLYGDIISDIVAQ
HHCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHH
IAGSVGMAGSSNIGEIVSMFEAIHGSAPDIAGKNLANPSGLLNAAVMMLVHIGQPDIAAK
HHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
INNAWLLTVEEGIHTGDIFKPGVSRIKVGTKEFADAVIGNLGHLPEKFKPVSFGKAKKII
ECCEEEEEEECCCCCCCCCCCCHHHEECCHHHHHHHHHHHHCCCCHHCCCCCCCCCCEEE
IPEYKRIIQKKELVGVDIFLDWIGNDPNELANKLKSLSNDLMLKIITNRGVKVYPEGQPE
CHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCC
TFLIDHWRCRFVSKNALIKQEDPSYHPISHKQVAELLLKLDSVGFDTIKTENLYYFNGKR
EEEEECEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCEEEECCCE
AFSLGQGE
EECCCCCC
>Mature Secondary Structure 
SEKTKIAIAHGDGIGPEIMDATLKILNAAGAKIDPIEIEIGERVYKDGHSSGIKPEAWD
CCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHCCCCCCCCHHHHH
LLRQTKVFLKAPITTPQGGGYKSLNVTVRTTLGLFANVRPCFSLYPYVETKHPSLDIVII
HHHHHHHHEECCCCCCCCCCEEEEEEEEEEHHHHHHCCCHHHHHCCCEECCCCCEEEEEE
RENEEDLYTGIEHRQTNDTVQCLKLISRPGSEKIIRYAFEYARAYGRKKVTAMVKDNIMK
ECCCHHHHHCCHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
QTDGLFHDIFKEIAQGYPELEANSQIIDIGAANLADRPQNFDVVVTLNLYGDIISDIVAQ
HHCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHH
IAGSVGMAGSSNIGEIVSMFEAIHGSAPDIAGKNLANPSGLLNAAVMMLVHIGQPDIAAK
HHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
INNAWLLTVEEGIHTGDIFKPGVSRIKVGTKEFADAVIGNLGHLPEKFKPVSFGKAKKII
ECCEEEEEEECCCCCCCCCCCCHHHEECCHHHHHHHHHHHHCCCCHHCCCCCCCCCCEEE
IPEYKRIIQKKELVGVDIFLDWIGNDPNELANKLKSLSNDLMLKIITNRGVKVYPEGQPE
CHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCC
TFLIDHWRCRFVSKNALIKQEDPSYHPISHKQVAELLLKLDSVGFDTIKTENLYYFNGKR
EEEEECEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCEEEECCCE
AFSLGQGE
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA