BacMap

Definition	Leptospira borgpetersenii serovar Hardjo-bovis L550 chromosome 1, complete sequence.
Accession	NC_008508
Length	3,614,446

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The map label for this gene is yvgN [H]

Identifier: 116327018

GI number: 116327018

Start: 221523

End: 222347

Strand: Reverse

Name: yvgN [H]

Synonym: LBL_0189

Alternate gene names: 116327018

Gene position: 222347-221523 (Counterclockwise)

Preceding gene: 116327019

Following gene: 116327016

Centisome position: 6.15

GC content: 37.09

Gene sequence:

>825_bases
ATGGAAAATGTATTAAACCAAGCTGTTACACTCAACAATGGACTTTCAATGCCCATCTTAGGACTTGGGGTTTGGAAAAC
CCAATCCGGTAATTGCAGGGAAGCAGTCTTAAACGCTTTAGAAGCGGGTTATCGTCATATCGATACCGCAAAAATTTATT
CTAATGAAGAAGACGTAGGTAAAGCGATTAAAGAAAGCGGAATTCCAAGAAAAGAGATTTTCATCACTACAAAACTTTGG
AATGCGGATCAAGGGTCGGACAAAACAAGAAAAGCTCTTGAAACCAGTCTCAAAAAATTAGGAATCGATCAGGTAGATCT
TTATCTCATTCATTTTCCGGTGACTTCCAAAAGGATGGATTCTTGGAAAGAATTAGAAAAAGCGTACCACGATAAACTCT
GTAAATCGATCGGGGTCAGTAATTATACGATTACTCATCTTACAGAGTTATTAAAAAATTCTGAAGCCACTCCTACAGTC
AATCAAGTGGAATTTCATCCTTTTTTAAACCAGGTCGATCTATTCGAATTTTGTAAAAAGCATAAGATTCAACTCGAAGC
TTACAGTCCTCTCGCTCACGGACAAAAAGTCGAAGATCCGAAGATCGCAGAAATTGCAAAAAAATACGGTAAAACACCGG
CGCAGATTTTGATCCGTTGGGCAATTGAACAAAAGATTGTGGTGATTCCAAAATCCACAAAAAAAGAAAGAATTGTTGAG
AACTCAAAAGTTTTCGATTTTAAAATTTCCGAAGAAGATATGAAAATTCTGAATTCTTTGGATGAAAATTTCAGAACCTG
TTGGGATCCTTCTGAAGTCGCTTAG

Upstream 100 bases:

>100_bases
TATTCTCTTATTCACGATATTATAGAATTATTGTGATGTTTTTATCTTTAAAAGTTTTGGTTCCGTTTCAGAGTCGTCCT
AATATCACGGAAATTCTACG

Downstream 100 bases:

>100_bases
GAATCGAAACTTATTTTACATACAAGACCAAAGTTTTCTATTAATGATTCGAAAACTGTATGATGAGTCTATACCAAAAT
CTTCGGATACTCCAATCGGA

Product: aldo/keto reductase

Products: NA

Alternate protein names: GR; Methylglyoxal reductase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MENVLNQAVTLNNGLSMPILGLGVWKTQSGNCREAVLNALEAGYRHIDTAKIYSNEEDVGKAIKESGIPRKEIFITTKLW
NADQGSDKTRKALETSLKKLGIDQVDLYLIHFPVTSKRMDSWKELEKAYHDKLCKSIGVSNYTITHLTELLKNSEATPTV
NQVEFHPFLNQVDLFEFCKKHKIQLEAYSPLAHGQKVEDPKIAEIAKKYGKTPAQILIRWAIEQKIVVIPKSTKKERIVE
NSKVFDFKISEEDMKILNSLDENFRTCWDPSEVA

Sequences:

>Translated_274_residues
MENVLNQAVTLNNGLSMPILGLGVWKTQSGNCREAVLNALEAGYRHIDTAKIYSNEEDVGKAIKESGIPRKEIFITTKLW
NADQGSDKTRKALETSLKKLGIDQVDLYLIHFPVTSKRMDSWKELEKAYHDKLCKSIGVSNYTITHLTELLKNSEATPTV
NQVEFHPFLNQVDLFEFCKKHKIQLEAYSPLAHGQKVEDPKIAEIAKKYGKTPAQILIRWAIEQKIVVIPKSTKKERIVE
NSKVFDFKISEEDMKILNSLDENFRTCWDPSEVA
>Mature_274_residues
MENVLNQAVTLNNGLSMPILGLGVWKTQSGNCREAVLNALEAGYRHIDTAKIYSNEEDVGKAIKESGIPRKEIFITTKLW
NADQGSDKTRKALETSLKKLGIDQVDLYLIHFPVTSKRMDSWKELEKAYHDKLCKSIGVSNYTITHLTELLKNSEATPTV
NQVEFHPFLNQVDLFEFCKKHKIQLEAYSPLAHGQKVEDPKIAEIAKKYGKTPAQILIRWAIEQKIVVIPKSTKKERIVE
NSKVFDFKISEEDMKILNSLDENFRTCWDPSEVA

Specific function: Reduces glyoxal and methylglyoxal. Is not involved in the vitamin B6 biosynthesis [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=293, Percent_Identity=37.8839590443686, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI5174391, Length=293, Percent_Identity=37.8839590443686, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI223468663, Length=295, Percent_Identity=40, Blast_Score=215, Evalue=3e-56,
Organism=Homo sapiens, GI310109922, Length=280, Percent_Identity=41.0714285714286, Blast_Score=205, Evalue=3e-53,
Organism=Homo sapiens, GI310109920, Length=300, Percent_Identity=38.6666666666667, Blast_Score=201, Evalue=4e-52,
Organism=Homo sapiens, GI45446745, Length=300, Percent_Identity=38.6666666666667, Blast_Score=201, Evalue=5e-52,
Organism=Homo sapiens, GI4503285, Length=300, Percent_Identity=38.6666666666667, Blast_Score=201, Evalue=5e-52,
Organism=Homo sapiens, GI5453543, Length=299, Percent_Identity=38.4615384615385, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI24497585, Length=299, Percent_Identity=38.4615384615385, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI4502049, Length=295, Percent_Identity=40.6779661016949, Blast_Score=197, Evalue=7e-51,
Organism=Homo sapiens, GI24497583, Length=299, Percent_Identity=37.4581939799331, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI5174695, Length=299, Percent_Identity=37.123745819398, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI93277124, Length=303, Percent_Identity=34.983498349835, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI291291012, Length=273, Percent_Identity=37.7289377289377, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI300116273, Length=278, Percent_Identity=36.6906474820144, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI300116271, Length=284, Percent_Identity=34.8591549295775, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI310109926, Length=131, Percent_Identity=45.0381679389313, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI207028673, Length=131, Percent_Identity=45.0381679389313, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=38.0645161290323, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI87082198, Length=270, Percent_Identity=46.2962962962963, Blast_Score=238, Evalue=3e-64,
Organism=Escherichia coli, GI1786400, Length=255, Percent_Identity=38.4313725490196, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1788081, Length=270, Percent_Identity=27.7777777777778, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787674, Length=245, Percent_Identity=24.8979591836735, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71998625, Length=276, Percent_Identity=42.3913043478261, Blast_Score=223, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI17537075, Length=300, Percent_Identity=36.6666666666667, Blast_Score=197, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17537077, Length=294, Percent_Identity=36.734693877551, Blast_Score=197, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17550248, Length=284, Percent_Identity=38.0281690140845, Blast_Score=191, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17552492, Length=278, Percent_Identity=36.6906474820144, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17566692, Length=285, Percent_Identity=37.5438596491228, Blast_Score=187, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17561298, Length=279, Percent_Identity=35.84229390681, Blast_Score=187, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17564128, Length=298, Percent_Identity=36.5771812080537, Blast_Score=184, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17562292, Length=282, Percent_Identity=34.0425531914894, Blast_Score=160, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17537079, Length=297, Percent_Identity=30.3030303030303, Blast_Score=160, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17538386, Length=271, Percent_Identity=32.4723247232472, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17561300, Length=276, Percent_Identity=33.695652173913, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17550246, Length=77, Percent_Identity=45.4545454545455, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320576, Length=282, Percent_Identity=41.8439716312057, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6324694, Length=303, Percent_Identity=38.9438943894389, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6321896, Length=311, Percent_Identity=36.0128617363344, Blast_Score=192, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6322556, Length=270, Percent_Identity=35.1851851851852, Blast_Score=188, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6320079, Length=294, Percent_Identity=36.734693877551, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319625, Length=309, Percent_Identity=36.2459546925566, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24663317, Length=288, Percent_Identity=38.8888888888889, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24644950, Length=301, Percent_Identity=38.2059800664452, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24657054, Length=281, Percent_Identity=40.9252669039146, Blast_Score=204, Evalue=4e-53,
Organism=Drosophila melanogaster, GI45553081, Length=293, Percent_Identity=37.2013651877133, Blast_Score=204, Evalue=7e-53,
Organism=Drosophila melanogaster, GI20129731, Length=290, Percent_Identity=38.2758620689655, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24662789, Length=292, Percent_Identity=35.958904109589, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI221378297, Length=321, Percent_Identity=35.8255451713396, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI21356425, Length=290, Percent_Identity=37.9310344827586, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24662781, Length=291, Percent_Identity=40.893470790378, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24662785, Length=291, Percent_Identity=39.5189003436426, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI281366140, Length=258, Percent_Identity=35.2713178294574, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.283 [H]

Molecular weight: Translated: 31205; Mature: 31205

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENVLNQAVTLNNGLSMPILGLGVWKTQSGNCREAVLNALEAGYRHIDTAKIYSNEEDVG
CCHHHHHHEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHCCHHHHH
KAIKESGIPRKEIFITTKLWNADQGSDKTRKALETSLKKLGIDQVDLYLIHFPVTSKRMD
HHHHHHCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHH
SWKELEKAYHDKLCKSIGVSNYTITHLTELLKNSEATPTVNQVEFHPFLNQVDLFEFCKK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHH
HKIQLEAYSPLAHGQKVEDPKIAEIAKKYGKTPAQILIRWAIEQKIVVIPKSTKKERIVE
CCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCCCCHHHHHC
NSKVFDFKISEEDMKILNSLDENFRTCWDPSEVA
CCCEEEEEECHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MENVLNQAVTLNNGLSMPILGLGVWKTQSGNCREAVLNALEAGYRHIDTAKIYSNEEDVG
CCHHHHHHEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHCCHHHHH
KAIKESGIPRKEIFITTKLWNADQGSDKTRKALETSLKKLGIDQVDLYLIHFPVTSKRMD
HHHHHHCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHH
SWKELEKAYHDKLCKSIGVSNYTITHLTELLKNSEATPTVNQVEFHPFLNQVDLFEFCKK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHH
HKIQLEAYSPLAHGQKVEDPKIAEIAKKYGKTPAQILIRWAIEQKIVVIPKSTKKERIVE
CCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCCCCHHHHHC
NSKVFDFKISEEDMKILNSLDENFRTCWDPSEVA
CCCEEEEEECHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]