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The map label for this gene is argG

Identifier: 116327008

GI number: 116327008

Start: 211261

End: 212472

Strand: Direct

Name: argG

Synonym: LBL_0178

Alternate gene names: 116327008

Gene position: 211261-212472 (Clockwise)

Preceding gene: 116327007

Following gene: 116327009

Centisome position: 5.84

GC content: 44.31

Gene sequence:

>1212_bases
ATGGCGCAAAGTAAACCTGTTAAAAAAATTGTCCTCGCCTATTCCGGCGGTTTGGATACGTCGGTAATTCTTACCTGGTT
GAAAGAAACGTATGGCTGCGAAGTAATCGCTTTTACTGCGGATGTAGGCCAAAAAGAAGAACTTTCCGGTTTGGAAGAGA
AGGGAATTAAAACCGGAGCTTCCAAAGTTTATATACAAGATCTCCGTTTGGAATTCGCTCGAGATTTTATTTTTCCTGCC
ATTCAAGGGAATGCACTTTATGAAATGCGTTATCTTCTCGGAACTTCTCTTGCCCGACCTCTTATCGCGAAGGCGATGGT
GGAAGTCGCCGAAAAGGAAGGCGCCGACGCTTTCGCTCACGGTGCGACCGGCAAAGGAAACGATCAGGTTCGTTTCGAAC
TCGGAGTTAAATCTTTAGCTCCCGAAAAAACAATCATAGCGCCTTGGAGAATCTGGAATTTCGGCGGCAGATCGGATTTG
ATCGAGTACGCAAAGTCCAAAGGGATTCCGGTTTCCATTACCGTGGAAAAGCCGTATTCTATGGACAGAAATCTAATGCA
TATTTCTTACGAAGGTGGAATATTAGAAGATCCTTATAGAGAACCGGATGAAAAAATGTTTCTCTTGACGACTTCTCCCG
AAAAAGCTCCCGACGCCCCCGAATATTTGGAACTCGACTTCCAAGAGGGGAATTGTGTCGCGATCAACGGTAAAAAACTG
AATCCTTACGAAGTGATGGAGGCTCTCAACACGATCGCGGGCAAACACGGAGTGGGTAGAGTGGACATCGTCGAAAATCG
CCTTGTGGGCATCAAGTCTAGGGGCGTTTATGAAACTCCGGGAGGAACGGTTTTATTTTTAGCTCACAGGGACCTGGAAT
CGATCACGATTGATCGGGATACACAACATCATAAAGATAAACTTTCCATTGAATTCGCCGAACTTATCTATAACGGCCAC
TGGTTTTCCTCAAGAATGAAAGCGGTTCGAGCTTTTATTACGGAAACTCAGAGATACGTAACCGGCGCCGTAAAAGTGAA
ACTCTATAAGGGAACTTGTTCAATTGTAGGTAGAAAATCTTCCGTTTCTCTCTACAATCCTAAAATGGCAACCTTTGAAA
AAGAGGAATTATATAACCAAAAAGACGCGGAAGGGTTTATCAATATCTACGGTTTACCTGCGCAAGAAACGGCTAGGCTT
CGTAAAAAATGA

Upstream 100 bases:

>100_bases
CGGTGTAGAAATTCATAACAGTGAAAAATGAAATCCTCATTATAGAAACCCGCAAAATCCGGTTGTCCACAGACCTGGAT
CGATTTTCAGTGTTCTCAGA

Downstream 100 bases:

>100_bases
AACATTTGGCGATTTATCCGGGTTCCTTTGATCCGTTAACAAACGGACATTTGGATATTCTTCAAAGGTCTTTGGGACTT
TTCGACAAGGTGATCATTGC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MAQSKPVKKIVLAYSGGLDTSVILTWLKETYGCEVIAFTADVGQKEELSGLEEKGIKTGASKVYIQDLRLEFARDFIFPA
IQGNALYEMRYLLGTSLARPLIAKAMVEVAEKEGADAFAHGATGKGNDQVRFELGVKSLAPEKTIIAPWRIWNFGGRSDL
IEYAKSKGIPVSITVEKPYSMDRNLMHISYEGGILEDPYREPDEKMFLLTTSPEKAPDAPEYLELDFQEGNCVAINGKKL
NPYEVMEALNTIAGKHGVGRVDIVENRLVGIKSRGVYETPGGTVLFLAHRDLESITIDRDTQHHKDKLSIEFAELIYNGH
WFSSRMKAVRAFITETQRYVTGAVKVKLYKGTCSIVGRKSSVSLYNPKMATFEKEELYNQKDAEGFINIYGLPAQETARL
RKK

Sequences:

>Translated_403_residues
MAQSKPVKKIVLAYSGGLDTSVILTWLKETYGCEVIAFTADVGQKEELSGLEEKGIKTGASKVYIQDLRLEFARDFIFPA
IQGNALYEMRYLLGTSLARPLIAKAMVEVAEKEGADAFAHGATGKGNDQVRFELGVKSLAPEKTIIAPWRIWNFGGRSDL
IEYAKSKGIPVSITVEKPYSMDRNLMHISYEGGILEDPYREPDEKMFLLTTSPEKAPDAPEYLELDFQEGNCVAINGKKL
NPYEVMEALNTIAGKHGVGRVDIVENRLVGIKSRGVYETPGGTVLFLAHRDLESITIDRDTQHHKDKLSIEFAELIYNGH
WFSSRMKAVRAFITETQRYVTGAVKVKLYKGTCSIVGRKSSVSLYNPKMATFEKEELYNQKDAEGFINIYGLPAQETARL
RKK
>Mature_402_residues
AQSKPVKKIVLAYSGGLDTSVILTWLKETYGCEVIAFTADVGQKEELSGLEEKGIKTGASKVYIQDLRLEFARDFIFPAI
QGNALYEMRYLLGTSLARPLIAKAMVEVAEKEGADAFAHGATGKGNDQVRFELGVKSLAPEKTIIAPWRIWNFGGRSDLI
EYAKSKGIPVSITVEKPYSMDRNLMHISYEGGILEDPYREPDEKMFLLTTSPEKAPDAPEYLELDFQEGNCVAINGKKLN
PYEVMEALNTIAGKHGVGRVDIVENRLVGIKSRGVYETPGGTVLFLAHRDLESITIDRDTQHHKDKLSIEFAELIYNGHW
FSSRMKAVRAFITETQRYVTGAVKVKLYKGTCSIVGRKSSVSLYNPKMATFEKEELYNQKDAEGFINIYGLPAQETARLR
KK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=403, Percent_Identity=51.8610421836228, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI16950633, Length=403, Percent_Identity=51.8610421836228, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1789563, Length=415, Percent_Identity=30.8433734939759, Blast_Score=161, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6324514, Length=399, Percent_Identity=45.6140350877193, Blast_Score=344, Evalue=2e-95,
Organism=Drosophila melanogaster, GI21358151, Length=407, Percent_Identity=46.1916461916462, Blast_Score=351, Evalue=4e-97,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_LEPBJ (Q04P84)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_802044.1
- ProteinModelPortal:   Q04P84
- SMR:   Q04P84
- STRING:   Q04P84
- GeneID:   4412155
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2885
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- PhylomeDB:   Q04P84
- ProtClustDB:   PRK00509
- BioCyc:   LBOR355277:LBJ_2885-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45010; Mature: 44879

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 40-40 BINDING 91-91 BINDING 96-96 BINDING 121-121 BINDING 123-123 BINDING 127-127 BINDING 127-127 BINDING 128-128 BINDING 131-131 BINDING 180-180 BINDING 189-189 BINDING 265-265 BINDING 277-277

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSKPVKKIVLAYSGGLDTSVILTWLKETYGCEVIAFTADVGQKEELSGLEEKGIKTGA
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHCCCHHHCCCCCC
SKVYIQDLRLEFARDFIFPAIQGNALYEMRYLLGTSLARPLIAKAMVEVAEKEGADAFAH
CEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
GATGKGNDQVRFELGVKSLAPEKTIIAPWRIWNFGGRSDLIEYAKSKGIPVSITVEKPYS
CCCCCCCCEEEEEECCHHCCCCCEEECCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCC
MDRNLMHISYEGGILEDPYREPDEKMFLLTTSPEKAPDAPEYLELDFQEGNCVAINGKKL
CCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCEEEEECCCC
NPYEVMEALNTIAGKHGVGRVDIVENRLVGIKSRGVYETPGGTVLFLAHRDLESITIDRD
CHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCEEECCCCEEEEEEECCCCEEEECCC
TQHHKDKLSIEFAELIYNGHWFSSRMKAVRAFITETQRYVTGAVKVKLYKGTCSIVGRKS
CCCCCHHHEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEECEEEEEECCC
SVSLYNPKMATFEKEELYNQKDAEGFINIYGLPAQETARLRKK
CEEEECCCCCCCCHHHHCCCCCCCCEEEEEECCHHHHHHHHCC
>Mature Secondary Structure 
AQSKPVKKIVLAYSGGLDTSVILTWLKETYGCEVIAFTADVGQKEELSGLEEKGIKTGA
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHCCCHHHCCCCCC
SKVYIQDLRLEFARDFIFPAIQGNALYEMRYLLGTSLARPLIAKAMVEVAEKEGADAFAH
CEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
GATGKGNDQVRFELGVKSLAPEKTIIAPWRIWNFGGRSDLIEYAKSKGIPVSITVEKPYS
CCCCCCCCEEEEEECCHHCCCCCEEECCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCC
MDRNLMHISYEGGILEDPYREPDEKMFLLTTSPEKAPDAPEYLELDFQEGNCVAINGKKL
CCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCEEEEECCCC
NPYEVMEALNTIAGKHGVGRVDIVENRLVGIKSRGVYETPGGTVLFLAHRDLESITIDRD
CHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCEEECCCCEEEEEEECCCCEEEECCC
TQHHKDKLSIEFAELIYNGHWFSSRMKAVRAFITETQRYVTGAVKVKLYKGTCSIVGRKS
CCCCCHHHEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEECEEEEEECCC
SVSLYNPKMATFEKEELYNQKDAEGFINIYGLPAQETARLRKK
CEEEECCCCCCCCHHHHCCCCCCCCEEEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA