Click here to switch to the map view.

The map label for this gene is hyfB-1 [H]

Identifier: 116326954

GI number: 116326954

Start: 137004

End: 138725

Strand: Reverse

Name: hyfB-1 [H]

Synonym: LBL_0120

Alternate gene names: 116326954

Gene position: 138725-137004 (Counterclockwise)

Preceding gene: 116326955

Following gene: 116326953

Centisome position: 3.84

GC content: 41.81

Gene sequence:

>1722_bases
ATGATCATTCTATCTTATTTGATAGTTGTAATGTCTCTTGCTGTACCCTTTTTAGCGGCAAAAGTACTCGGCAAGGATTT
TTTCGGACAGGATTCGGCTATATTTATCGGCCTTACTTTGCAGACGGTGTTGGGAACGATAATCTCCGTTTATGTTCACG
GTTATGAACATCGTAAAAGAACAACGATTCTTTTGGGCTACGCTATATTCTTTCTCAGTACTGGACTATGCTATTTAGTC
GGTAAAACTTTATGGCTGATTCTCTTTTGGGAATTGTCCACGGTTAGCGCTTTCCTTCTTTATCAAGGAGGCAAGTGGAC
TCCGAATTCCATCAAAAGTTTTATTGCGTTAGTCGTTGCGGGTGGGGTCGGGGCTTTTTGTTTTACGTATTGGATTTATT
CTCCTGAAGGCCAGTTCGGAAATATTTTTTTGGTTGCCGGTCTTTTGGTGAAAGTGGCATTTTTTGGGTTTCACTTCTGG
CTTCCGGAAGCTCACTCCGGATCTCCGGCTCATGCGTCTGCCGCTTACTCGGGAATTTTAGTAAATCTTCCTTTGATCCT
TTTTCATCAGCTTGTGGCTCCTTGGCTTGGTGATACTGTTTATATCAAAATTTTGATTCCTTTAGCAGGTTTCGGAGTAC
TTTGGGCGGGTTTATCTTCTTTATTTTCAAAAGATGCTAAGAAGGCGATTGCTTACAGTACTGTAGAGAATATGAATTTT
CTATTTCTTTGTATTCTTCTTTCTGCGTTGTGGAAAGACAGCGAAGTGGAAAATTTAAAAGTACTCAGCAGATCTTTTTC
GTTTCTTTTCATTCTTTCTTTGATCCATCATAGTATCAGTAAGACGTTTCAGTTTCTTTCAGTCGGGTACCTTACCAAAA
TATCAGGTTTTTCGAATGTAGATCAAAACACCGGTGTCGGAAGAGTTTCCGGGTTACCGTCTTCGTTATTGAGTTTAGGG
ACGATCAGTTTTCTCGCGATTCCGGGAACGACCGGGTTTCTTTCCGAGGCGACGTTCGTAAAATTGGTCGCGGGAGTTTT
GGAAATTCCAGGGCAAAGCGCGATTCTTGTGCTTCCACTTCTGATTTTAGTTTCTTCCGGTTTAGTCTTAGGAGCGGCAG
GACACTTGAGAATTTCTTTAGGAATGGTTTTGTCCAGACCGAGAACCGGATGGTCTGAAAACAAACCTTTTCGCTCCGTA
TTCTATTCTATTTTTCTGAGCGGCGCTTTGATTTTAGTCGTGTCCTTTGGAATCTCCGCCTATGCGCTGTATTATTCGCC
AACTACGGAATGGTTGGACGAACAGTGGTATAGAAGTCTTGCAATCGTCAATTTTGTTGGACTTGGAATGATCGTTTTTG
TGGGAATTATAGGTTTCAGAACAAAAATTGCCAAAAGGAAACTTTGGGATTGTGGCGGTAATTACGGCGGCGCGGACGTT
GCGGTTCCTTCCGACGGAATCTCGGATCCTCTTTTCCCTTCCCTGGGAAGATATTTTACCAATGAGGAAGGAAACTCTCG
TTTAGACGAAGGGATACTTCAGGGAATTATAAAATTCTTAAATACTTTTAAGACGAGAGTGAACGAGGCAGATGAAGAAT
CGATTTCGATCAACCTTGCATTTTCGTCCGTAACCGTGGTGGTCCTTCTGTTTGTGATTATCGGTCTGAAATTAACCGAG
GGGGATTTATGGAAATTATTCTTAAATACTTTGATCCGATAA

Upstream 100 bases:

>100_bases
CTGCGGCGTTGATGAAGCAGCATACCTCCGAAAAATACGCGAGCGTATTCTTCTTTCAGATGAACGCCGCGCTTTAGCAA
AAAGGAGGCTCAATCAGCCA

Downstream 100 bases:

>100_bases
TTCGAATAGTATCGTTTTTTGCTCTCCCATTTTTATGTGGAGGACTTGTTCAAAAGATACGTTCTTATTCGCAAGGAAGA
AAGGGCACTCCTGTTCTTCA

Product: formate hydrogenase, subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MIILSYLIVVMSLAVPFLAAKVLGKDFFGQDSAIFIGLTLQTVLGTIISVYVHGYEHRKRTTILLGYAIFFLSTGLCYLV
GKTLWLILFWELSTVSAFLLYQGGKWTPNSIKSFIALVVAGGVGAFCFTYWIYSPEGQFGNIFLVAGLLVKVAFFGFHFW
LPEAHSGSPAHASAAYSGILVNLPLILFHQLVAPWLGDTVYIKILIPLAGFGVLWAGLSSLFSKDAKKAIAYSTVENMNF
LFLCILLSALWKDSEVENLKVLSRSFSFLFILSLIHHSISKTFQFLSVGYLTKISGFSNVDQNTGVGRVSGLPSSLLSLG
TISFLAIPGTTGFLSEATFVKLVAGVLEIPGQSAILVLPLLILVSSGLVLGAAGHLRISLGMVLSRPRTGWSENKPFRSV
FYSIFLSGALILVVSFGISAYALYYSPTTEWLDEQWYRSLAIVNFVGLGMIVFVGIIGFRTKIAKRKLWDCGGNYGGADV
AVPSDGISDPLFPSLGRYFTNEEGNSRLDEGILQGIIKFLNTFKTRVNEADEESISINLAFSSVTVVVLLFVIIGLKLTE
GDLWKLFLNTLIR

Sequences:

>Translated_573_residues
MIILSYLIVVMSLAVPFLAAKVLGKDFFGQDSAIFIGLTLQTVLGTIISVYVHGYEHRKRTTILLGYAIFFLSTGLCYLV
GKTLWLILFWELSTVSAFLLYQGGKWTPNSIKSFIALVVAGGVGAFCFTYWIYSPEGQFGNIFLVAGLLVKVAFFGFHFW
LPEAHSGSPAHASAAYSGILVNLPLILFHQLVAPWLGDTVYIKILIPLAGFGVLWAGLSSLFSKDAKKAIAYSTVENMNF
LFLCILLSALWKDSEVENLKVLSRSFSFLFILSLIHHSISKTFQFLSVGYLTKISGFSNVDQNTGVGRVSGLPSSLLSLG
TISFLAIPGTTGFLSEATFVKLVAGVLEIPGQSAILVLPLLILVSSGLVLGAAGHLRISLGMVLSRPRTGWSENKPFRSV
FYSIFLSGALILVVSFGISAYALYYSPTTEWLDEQWYRSLAIVNFVGLGMIVFVGIIGFRTKIAKRKLWDCGGNYGGADV
AVPSDGISDPLFPSLGRYFTNEEGNSRLDEGILQGIIKFLNTFKTRVNEADEESISINLAFSSVTVVVLLFVIIGLKLTE
GDLWKLFLNTLIR
>Mature_573_residues
MIILSYLIVVMSLAVPFLAAKVLGKDFFGQDSAIFIGLTLQTVLGTIISVYVHGYEHRKRTTILLGYAIFFLSTGLCYLV
GKTLWLILFWELSTVSAFLLYQGGKWTPNSIKSFIALVVAGGVGAFCFTYWIYSPEGQFGNIFLVAGLLVKVAFFGFHFW
LPEAHSGSPAHASAAYSGILVNLPLILFHQLVAPWLGDTVYIKILIPLAGFGVLWAGLSSLFSKDAKKAIAYSTVENMNF
LFLCILLSALWKDSEVENLKVLSRSFSFLFILSLIHHSISKTFQFLSVGYLTKISGFSNVDQNTGVGRVSGLPSSLLSLG
TISFLAIPGTTGFLSEATFVKLVAGVLEIPGQSAILVLPLLILVSSGLVLGAAGHLRISLGMVLSRPRTGWSENKPFRSV
FYSIFLSGALILVVSFGISAYALYYSPTTEWLDEQWYRSLAIVNFVGLGMIVFVGIIGFRTKIAKRKLWDCGGNYGGADV
AVPSDGISDPLFPSLGRYFTNEEGNSRLDEGILQGIIKFLNTFKTRVNEADEESISINLAFSSVTVVVLLFVIIGLKLTE
GDLWKLFLNTLIR

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Escherichia coli, GI1788831, Length=259, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 62600; Mature: 62600

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]