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The map label for this gene is purT

Identifier: 116326946

GI number: 116326946

Start: 125928

End: 127091

Strand: Reverse

Name: purT

Synonym: LBL_0112

Alternate gene names: 116326946

Gene position: 127091-125928 (Counterclockwise)

Preceding gene: 116326947

Following gene: 116326944

Centisome position: 3.52

GC content: 40.98

Gene sequence:

>1164_bases
ATGAAAAAGAAAATTCTCCTTTTAGGATCCGGGGAACTCGGCAAAGAATTCATCATCGCGGCACAAAGACTAGGGCAATA
CGTAATTGCCGTTGATTTTTATGACGGCGCGCCTGCCATGCAGGTTGCTCATGAAAAAGAAATCATCAACATGCTAGATG
GGAACCTTCTGGATCAAGTCGTAAAAAAACATAAACCGGATCTAATCGTTCCCGAAATCGAAGCGATTAGAACCGAACGT
TTCTACGAATATGAAAAACAAGGTTACCAGATAGTTCCCTCTGCTAAAGCCGCTAATTTCACAATGAACCGGAAATCGAT
CCGTGATTTGGCAGCAAAAGATTTAAAACTTCTCACTGCAAAATATTTATATGCTTCTTCGGAAAAAGAACTAGCAAAAG
CAACCAAAATATTAGGTTTTCCTTGTGTAGTTAAACCTCTCATGTCTTCTTCCGGAAAAGGGCAGTCAATCATCAAATCC
CCGAAAGATATTTCCAAAGCATGGGAAGCTTCTCAAACTAAAGGCCGTACGAGCGCTACGGAAATTATCGTGGAAGAATT
TATTTCTTTCAAATCGGAGATTACACTTCTAACAGTTACGCAGAAAAATGGCAAAACGCTTTTCTGTCCGCCGATCGGTC
ATAGGCAGGAAAGAGGAGATTATCAAGAAAGTTGGCAACCGGCAGAAATTTCCGAAGTTCAATTGAAGGAAGCACAAAGA
ATGGCGGAAGCAGTAACAAAAGAACTAACAGGTTTCGGTATTTGGGGCGTGGAATTTTTTTTAACTGAAGATCAAGTTTA
TTTTTCAGAACTTTCTCCGAGACCACACGATACCGGTATGGTCACGTTATCCGGAACGCAAAACTTCAACGAGTTTGAAC
TTCATATTCGTGCGATTTTAGGAATCCCGATTCCCGAAGTCACTCAAGAAAGAAAAGGTGCAAGCGCAGTAATTCTTGCG
AGTACGGACGGTAAAATTCCGGAAGTAAGAGGATTAGATATCGCATCGGAAATGCCCGAATCAGATTTTAGAATTTTCGG
AAAACCGATTACAAGACCTTATCGTAGGATGGGAATCGCCCTTTCCTATTCCACAAAAGGAGAAGGAATTTCCTCCCTTC
GTAAACGTGCGATTCTTTTGGCTTCTAAAATTAAAGTAGACTAA

Upstream 100 bases:

>100_bases
TCTCGGAAAGTTATTCTATGATGTAATTGCAATTTTTGGGACAGATTAAGATTTTCTTTTTGATCTTTCGAATATTTCTT
TCAGTCTGCCGATAAAACCT

Downstream 100 bases:

>100_bases
GAGCGTGTCCCAAAACTGTCTTCTGTGGGAGCGGCATTGCCGCGACTCTGTCCTCAGTCCTCTGAATGTGGAAGTTCCCA
CAGAAACCGTCGTATTGAAG

Product: phosphoribosylglycinamide formyltransferase 2

Products: tetrahydrofolate; N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKKKILLLGSGELGKEFIIAAQRLGQYVIAVDFYDGAPAMQVAHEKEIINMLDGNLLDQVVKKHKPDLIVPEIEAIRTER
FYEYEKQGYQIVPSAKAANFTMNRKSIRDLAAKDLKLLTAKYLYASSEKELAKATKILGFPCVVKPLMSSSGKGQSIIKS
PKDISKAWEASQTKGRTSATEIIVEEFISFKSEITLLTVTQKNGKTLFCPPIGHRQERGDYQESWQPAEISEVQLKEAQR
MAEAVTKELTGFGIWGVEFFLTEDQVYFSELSPRPHDTGMVTLSGTQNFNEFELHIRAILGIPIPEVTQERKGASAVILA
STDGKIPEVRGLDIASEMPESDFRIFGKPITRPYRRMGIALSYSTKGEGISSLRKRAILLASKIKVD

Sequences:

>Translated_387_residues
MKKKILLLGSGELGKEFIIAAQRLGQYVIAVDFYDGAPAMQVAHEKEIINMLDGNLLDQVVKKHKPDLIVPEIEAIRTER
FYEYEKQGYQIVPSAKAANFTMNRKSIRDLAAKDLKLLTAKYLYASSEKELAKATKILGFPCVVKPLMSSSGKGQSIIKS
PKDISKAWEASQTKGRTSATEIIVEEFISFKSEITLLTVTQKNGKTLFCPPIGHRQERGDYQESWQPAEISEVQLKEAQR
MAEAVTKELTGFGIWGVEFFLTEDQVYFSELSPRPHDTGMVTLSGTQNFNEFELHIRAILGIPIPEVTQERKGASAVILA
STDGKIPEVRGLDIASEMPESDFRIFGKPITRPYRRMGIALSYSTKGEGISSLRKRAILLASKIKVD
>Mature_387_residues
MKKKILLLGSGELGKEFIIAAQRLGQYVIAVDFYDGAPAMQVAHEKEIINMLDGNLLDQVVKKHKPDLIVPEIEAIRTER
FYEYEKQGYQIVPSAKAANFTMNRKSIRDLAAKDLKLLTAKYLYASSEKELAKATKILGFPCVVKPLMSSSGKGQSIIKS
PKDISKAWEASQTKGRTSATEIIVEEFISFKSEITLLTVTQKNGKTLFCPPIGHRQERGDYQESWQPAEISEVQLKEAQR
MAEAVTKELTGFGIWGVEFFLTEDQVYFSELSPRPHDTGMVTLSGTQNFNEFELHIRAILGIPIPEVTQERKGASAVILA
STDGKIPEVRGLDIASEMPESDFRIFGKPITRPYRRMGIALSYSTKGEGISSLRKRAILLASKIKVD

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=389, Percent_Identity=48.0719794344473, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1786733, Length=308, Percent_Identity=25.3246753246753, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=363, Percent_Identity=26.1707988980716, Blast_Score=112, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_LEPBJ (Q04P21)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_802107.1
- ProteinModelPortal:   Q04P21
- STRING:   Q04P21
- GeneID:   4411578
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2952
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   TMNRKAI
- PhylomeDB:   Q04P21
- ProtClustDB:   PRK09288
- BioCyc:   LBOR355277:LBJ_2952-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.2

Molecular weight: Translated: 43164; Mature: 43164

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 72-72 BINDING 104-104 BINDING 145-145 BINDING 193-193 BINDING 258-258 BINDING 270-270 BINDING 277-277 BINDING 348-348

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKILLLGSGELGKEFIIAAQRLGQYVIAVDFYDGAPAMQVAHEKEIINMLDGNLLDQV
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHH
VKKHKPDLIVPEIEAIRTERFYEYEKQGYQIVPSAKAANFTMNRKSIRDLAAKDLKLLTA
HHHCCCCEECCCHHHHHHHHHHHHHHCCCEEECCCCCCCEEECHHHHHHHHHHHHHHHHH
KYLYASSEKELAKATKILGFPCVVKPLMSSSGKGQSIIKSPKDISKAWEASQTKGRTSAT
HHHHCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHCCCCHHHH
EIIVEEFISFKSEITLLTVTQKNGKTLFCPPIGHRQERGDYQESWQPAEISEVQLKEAQR
HHHHHHHHHHCCCEEEEEEEECCCCEEEECCCCCCHHCCCHHHCCCCCCHHHHHHHHHHH
MAEAVTKELTGFGIWGVEFFLTEDQVYFSELSPRPHDTGMVTLSGTQNFNEFELHIRAIL
HHHHHHHHHCCCEEEEEEEEEECCCCHHHHCCCCCCCCEEEEEECCCCCCHHHEEEEEEE
GIPIPEVTQERKGASAVILASTDGKIPEVRGLDIASEMPESDFRIFGKPITRPYRRMGIA
CCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCEEECCCCCCHHHHHCEE
LSYSTKGEGISSLRKRAILLASKIKVD
EEECCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKKILLLGSGELGKEFIIAAQRLGQYVIAVDFYDGAPAMQVAHEKEIINMLDGNLLDQV
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHH
VKKHKPDLIVPEIEAIRTERFYEYEKQGYQIVPSAKAANFTMNRKSIRDLAAKDLKLLTA
HHHCCCCEECCCHHHHHHHHHHHHHHCCCEEECCCCCCCEEECHHHHHHHHHHHHHHHHH
KYLYASSEKELAKATKILGFPCVVKPLMSSSGKGQSIIKSPKDISKAWEASQTKGRTSAT
HHHHCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHCCCCHHHH
EIIVEEFISFKSEITLLTVTQKNGKTLFCPPIGHRQERGDYQESWQPAEISEVQLKEAQR
HHHHHHHHHHCCCEEEEEEEECCCCEEEECCCCCCHHCCCHHHCCCCCCHHHHHHHHHHH
MAEAVTKELTGFGIWGVEFFLTEDQVYFSELSPRPHDTGMVTLSGTQNFNEFELHIRAIL
HHHHHHHHHCCCEEEEEEEEEECCCCHHHHCCCCCCCCEEEEEECCCCCCHHHEEEEEEE
GIPIPEVTQERKGASAVILASTDGKIPEVRGLDIASEMPESDFRIFGKPITRPYRRMGIA
CCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCEEECCCCCCHHHHHCEE
LSYSTKGEGISSLRKRAILLASKIKVD
EEECCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 10-formyltetrahydrofolate; N1-(5-phospho-D-ribosyl)glycinamide

Specific reaction: 10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA