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The map label for this gene is vacB [H]

Identifier: 116326913

GI number: 116326913

Start: 85207

End: 87474

Strand: Reverse

Name: vacB [H]

Synonym: LBL_0073

Alternate gene names: 116326913

Gene position: 87474-85207 (Counterclockwise)

Preceding gene: 116326914

Following gene: 116326912

Centisome position: 2.42

GC content: 38.89

Gene sequence:

>2268_bases
ATGAATATAAAGAAAAAACAAACAAAACAAAACAAAACCGCAAAACCGGATACGAAAAAGAAAATCTTCGGCCGATCGGA
TAAGAATAAAAAAAACAGATTCGCGAGAAGATCCGAAGGATTCCCGGAAAGTGAAATCGGCAGGAAAATCCTCAGGTATC
TTCGATCCAGAGCCGGTTCCGTGATCCAATTTAAGGATCTGACCGCAAAGATTCTTAAGGAAGAAAATCAAAATTCTTAC
GGTAAAAAAAGAGAGAAATGGCAATTTGAAGAAAGAGAAAGGGAAATAACCGAAATACTTCAAATTCTTGAGGCAGAAGG
GTTGATTGAATTAGAAAAAAAGAATATTCTCGTCAATTCAAATCAAAAGCTGCTAGGGACGATTTCGACCAGTAAAAAAG
GGGACGGTTTCGTGAAACTTCCTTCCGGAATGGAGGTATTTGTTCCGGGCCAATACGTACAGTCGGCGATTCAAGGAGAT
CTTGTAGAAGTTCAGCCGAACGCAATCGGAAAAAAAGGAAGATTGGAAGGAGAGGTCACAAAGATTCTCAGAAGGGGGCG
TGATCTTTACAGAATGATCGTCATTGAAAAGGATCCTAAATTTATTTTTGGAAAACTCTTAGACATAGATGGAGAAGAAA
AGGAGGGGTATCTTCTTCGTAAAACCATTCTTATGGATCTTCAAGATGAGATCCAACTCGGGGATGTTCTGATCGTAAAA
CTCAGGGAAGAAACGGAACACGAAAGAAATCTCTACGAAGTTCAGTTTCTACGTTTTGAATCTGATACCAAAGAAGATTT
GGATCTTATGAGAATGTTAATGAAATACAATTATAGCATTCTTTATCCTGAAAATATAGTACTGAATCTTCCGGAAGAAG
TGGACGAAAGCACCGTAGATGATTGGGGATCTCGCGTGGATCTTAGAAATTTGAAATGTATCACGATTGACGGAGAATAT
TCCAAAGACTTCGACGATGCGATCAGTTTTGTAGAAGAAAAAAGTAGGATTCGATTTTACGTTCATGTTGCAGACGTTTC
TTACTACGTAAGACTGGGAACCGATTTGGACGAAGAGGCGTACAATCGAGCGACTTCCGTATATTTAGGAAGTAGAGTTG
TTCCTATGTTACCTCCGGATTTGTCCGAAAACCTTTGTTCTTTAGTTGCGGGGAAAAATCGTTTAGCCTTTACGGTGGAA
ATGGAAGCTGATTGGAAAGGAAAAATCACTCATGTTAAATATTATAAAAGTGTAATTAATGTTGCGGAAAGATATACGTA
TAACCGGGCGGAGTCCGAAATCCTATCAGGTGACCCTGGTAATTGGATTTTCAGAATGAACGAATTCGCCAAAGCTCTTC
GAGCCAGAAGGATTGAAGATGGAAGAGTGGATTTAAATTTAAAGGAAAATAAAGTAGTCACCGATTCCGAACACAACGTA
GTCGAGATCTCCGTACAGGACAGGTTGCAAGCGCATATTCTCATTGAAGAATTTATGCTTTCGGCAAATATTAAGATTGC
TGAATCGATTCGCAAGAAAAAGCATCCCACCTTGTATCGCATTCACGAGCCAATGAACGAGGAAAAGCTCGAGTCTCTCA
ACGCATTTTTGCAGCTGAACGGAATTAAGATTTTATTGAAAGATTCAAGTTACGAAGCGATTCGGGCGGTTTTGAAAGAA
TTGGAAGGAAAACCTGCGGAAAGGTTGTTCAATATGTTTTTGCTCCGAACGTTTATGCAGGCTTATTATTCCGGAGAATA
TCTCGGGCATTGGGGTTTGGGTTTTGAGGATTATTGTCACTTTACTTCTCCGATCCGAAGATATCCTGATTTAGTTTGTC
ATCGGGTTTTACAACAAATTCTTTTGGGTAAAAAACCTCTTTATGGTCCGGAAGAAGTGGTGACTTTGGGTTTGCATTGT
TCCCATCAGGAACGAAAGGCGAGTGACGCTGAACGAGATTATTACAAACTCAAAGCGTGTCGTTATTTGGAAAAAACGGG
CATCAAAGAGTTTTCTGCAACGATTACCGGTTGTAAACCGCATTTGATTTTCGTGGATCTGGAAAATCCTTCCGTAGACG
CTTGTTTAATTTCTTCTGAATTTACGGATGAAGGTGAAATTCGATTGGAGACGGATTTTTCGTTTTATTCGAAAAAATAT
TCTAAAATTTATACACTTGGAGACAAGATCGAAGTTGAACTTGATCGAGTCGATTATGAGGAGATTAAAATCTTTGTAAA
AATGAAAAAATTCCAAAAAAAAGTATAA

Upstream 100 bases:

>100_bases
ATGAAGCTTTAGAATTTACGGATCGATTTCCATATTGGAAACTGACGCCGAATTATATACGTGGAACCTACGTCGACGAC
AAACCGATAGTTTAGTAATT

Downstream 100 bases:

>100_bases
GAAGCTGTCGCTCTCTAAGGATCGCTTCCGCTATGAAGTTGGTGGGCGATTCTCCCGAACTTTCTTATATCGAATTCACG
TTATTTACGGCTTCAATTGA

Product: exoribonuclease R

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 755; Mature: 755

Protein sequence:

>755_residues
MNIKKKQTKQNKTAKPDTKKKIFGRSDKNKKNRFARRSEGFPESEIGRKILRYLRSRAGSVIQFKDLTAKILKEENQNSY
GKKREKWQFEEREREITEILQILEAEGLIELEKKNILVNSNQKLLGTISTSKKGDGFVKLPSGMEVFVPGQYVQSAIQGD
LVEVQPNAIGKKGRLEGEVTKILRRGRDLYRMIVIEKDPKFIFGKLLDIDGEEKEGYLLRKTILMDLQDEIQLGDVLIVK
LREETEHERNLYEVQFLRFESDTKEDLDLMRMLMKYNYSILYPENIVLNLPEEVDESTVDDWGSRVDLRNLKCITIDGEY
SKDFDDAISFVEEKSRIRFYVHVADVSYYVRLGTDLDEEAYNRATSVYLGSRVVPMLPPDLSENLCSLVAGKNRLAFTVE
MEADWKGKITHVKYYKSVINVAERYTYNRAESEILSGDPGNWIFRMNEFAKALRARRIEDGRVDLNLKENKVVTDSEHNV
VEISVQDRLQAHILIEEFMLSANIKIAESIRKKKHPTLYRIHEPMNEEKLESLNAFLQLNGIKILLKDSSYEAIRAVLKE
LEGKPAERLFNMFLLRTFMQAYYSGEYLGHWGLGFEDYCHFTSPIRRYPDLVCHRVLQQILLGKKPLYGPEEVVTLGLHC
SHQERKASDAERDYYKLKACRYLEKTGIKEFSATITGCKPHLIFVDLENPSVDACLISSEFTDEGEIRLETDFSFYSKKY
SKIYTLGDKIEVELDRVDYEEIKIFVKMKKFQKKV

Sequences:

>Translated_755_residues
MNIKKKQTKQNKTAKPDTKKKIFGRSDKNKKNRFARRSEGFPESEIGRKILRYLRSRAGSVIQFKDLTAKILKEENQNSY
GKKREKWQFEEREREITEILQILEAEGLIELEKKNILVNSNQKLLGTISTSKKGDGFVKLPSGMEVFVPGQYVQSAIQGD
LVEVQPNAIGKKGRLEGEVTKILRRGRDLYRMIVIEKDPKFIFGKLLDIDGEEKEGYLLRKTILMDLQDEIQLGDVLIVK
LREETEHERNLYEVQFLRFESDTKEDLDLMRMLMKYNYSILYPENIVLNLPEEVDESTVDDWGSRVDLRNLKCITIDGEY
SKDFDDAISFVEEKSRIRFYVHVADVSYYVRLGTDLDEEAYNRATSVYLGSRVVPMLPPDLSENLCSLVAGKNRLAFTVE
MEADWKGKITHVKYYKSVINVAERYTYNRAESEILSGDPGNWIFRMNEFAKALRARRIEDGRVDLNLKENKVVTDSEHNV
VEISVQDRLQAHILIEEFMLSANIKIAESIRKKKHPTLYRIHEPMNEEKLESLNAFLQLNGIKILLKDSSYEAIRAVLKE
LEGKPAERLFNMFLLRTFMQAYYSGEYLGHWGLGFEDYCHFTSPIRRYPDLVCHRVLQQILLGKKPLYGPEEVVTLGLHC
SHQERKASDAERDYYKLKACRYLEKTGIKEFSATITGCKPHLIFVDLENPSVDACLISSEFTDEGEIRLETDFSFYSKKY
SKIYTLGDKIEVELDRVDYEEIKIFVKMKKFQKKV
>Mature_755_residues
MNIKKKQTKQNKTAKPDTKKKIFGRSDKNKKNRFARRSEGFPESEIGRKILRYLRSRAGSVIQFKDLTAKILKEENQNSY
GKKREKWQFEEREREITEILQILEAEGLIELEKKNILVNSNQKLLGTISTSKKGDGFVKLPSGMEVFVPGQYVQSAIQGD
LVEVQPNAIGKKGRLEGEVTKILRRGRDLYRMIVIEKDPKFIFGKLLDIDGEEKEGYLLRKTILMDLQDEIQLGDVLIVK
LREETEHERNLYEVQFLRFESDTKEDLDLMRMLMKYNYSILYPENIVLNLPEEVDESTVDDWGSRVDLRNLKCITIDGEY
SKDFDDAISFVEEKSRIRFYVHVADVSYYVRLGTDLDEEAYNRATSVYLGSRVVPMLPPDLSENLCSLVAGKNRLAFTVE
MEADWKGKITHVKYYKSVINVAERYTYNRAESEILSGDPGNWIFRMNEFAKALRARRIEDGRVDLNLKENKVVTDSEHNV
VEISVQDRLQAHILIEEFMLSANIKIAESIRKKKHPTLYRIHEPMNEEKLESLNAFLQLNGIKILLKDSSYEAIRAVLKE
LEGKPAERLFNMFLLRTFMQAYYSGEYLGHWGLGFEDYCHFTSPIRRYPDLVCHRVLQQILLGKKPLYGPEEVVTLGLHC
SHQERKASDAERDYYKLKACRYLEKTGIKEFSATITGCKPHLIFVDLENPSVDACLISSEFTDEGEIRLETDFSFYSKKY
SKIYTLGDKIEVELDRVDYEEIKIFVKMKKFQKKV

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014623, Length=399, Percent_Identity=32.0802005012531, Blast_Score=167, Evalue=4e-41,
Organism=Homo sapiens, GI190014625, Length=369, Percent_Identity=33.0623306233062, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI134288890, Length=473, Percent_Identity=28.7526427061311, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI19115966, Length=420, Percent_Identity=27.3809523809524, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI219521928, Length=421, Percent_Identity=27.3159144893112, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI156105695, Length=326, Percent_Identity=24.5398773006135, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI156105693, Length=317, Percent_Identity=24.6056782334385, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI87082383, Length=657, Percent_Identity=30.5936073059361, Blast_Score=269, Evalue=4e-73,
Organism=Escherichia coli, GI1787542, Length=590, Percent_Identity=24.5762711864407, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI212645896, Length=407, Percent_Identity=31.4496314496315, Blast_Score=152, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17553506, Length=412, Percent_Identity=27.4271844660194, Blast_Score=145, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6324552, Length=484, Percent_Identity=32.0247933884297, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320499, Length=340, Percent_Identity=25, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323943, Length=380, Percent_Identity=24.7368421052632, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24649634, Length=351, Percent_Identity=33.3333333333333, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI19922976, Length=409, Percent_Identity=29.0953545232274, Blast_Score=134, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24654597, Length=409, Percent_Identity=29.0953545232274, Blast_Score=134, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24654592, Length=409, Percent_Identity=29.0953545232274, Blast_Score=134, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805
- InterPro:   IPR011991 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 87849; Mature: 87849

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKKKQTKQNKTAKPDTKKKIFGRSDKNKKNRFARRSEGFPESEIGRKILRYLRSRAGS
CCCCHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
VIQFKDLTAKILKEENQNSYGKKREKWQFEEREREITEILQILEAEGLIELEKKNILVNS
EEEEHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEC
NQKLLGTISTSKKGDGFVKLPSGMEVFVPGQYVQSAIQGDLVEVQPNAIGKKGRLEGEVT
CCEEEEEEECCCCCCCEEECCCCCEEEECHHHHHHHHCCCEEEECCCCCCCCCCCCHHHH
KILRRGRDLYRMIVIEKDPKFIFGKLLDIDGEEKEGYLLRKTILMDLQDEIQLGDVLIVK
HHHHCCCCEEEEEEEECCCCEEEHEEECCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEE
LREETEHERNLYEVQFLRFESDTKEDLDLMRMLMKYNYSILYPENIVLNLPEEVDESTVD
EHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCEEECCCCEEEECCHHHCHHHHH
DWGSRVDLRNLKCITIDGEYSKDFDDAISFVEEKSRIRFYVHVADVSYYVRLGTDLDEEA
HHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEECEEEEEEECCCCCHHH
YNRATSVYLGSRVVPMLPPDLSENLCSLVAGKNRLAFTVEMEADWKGKITHVKYYKSVIN
HHHHHHEEECCEECCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHH
VAERYTYNRAESEILSGDPGNWIFRMNEFAKALRARRIEDGRVDLNLKENKVVTDSEHNV
HHHHHHCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCE
VEISVQDRLQAHILIEEFMLSANIKIAESIRKKKHPTLYRIHEPMNEEKLESLNAFLQLN
EEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHEEEC
GIKILLKDSSYEAIRAVLKELEGKPAERLFNMFLLRTFMQAYYSGEYLGHWGLGFEDYCH
CEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
FTSPIRRYPDLVCHRVLQQILLGKKPLYGPEEVVTLGLHCSHQERKASDAERDYYKLKAC
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCHHHHCCCHHHHHHHHHHH
RYLEKTGIKEFSATITGCKPHLIFVDLENPSVDACLISSEFTDEGEIRLETDFSFYSKKY
HHHHHCCCHHHHHEEECCCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEECHHHHHHHH
SKIYTLGDKIEVELDRVDYEEIKIFVKMKKFQKKV
HHEEEECCEEEEEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIKKKQTKQNKTAKPDTKKKIFGRSDKNKKNRFARRSEGFPESEIGRKILRYLRSRAGS
CCCCHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
VIQFKDLTAKILKEENQNSYGKKREKWQFEEREREITEILQILEAEGLIELEKKNILVNS
EEEEHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEC
NQKLLGTISTSKKGDGFVKLPSGMEVFVPGQYVQSAIQGDLVEVQPNAIGKKGRLEGEVT
CCEEEEEEECCCCCCCEEECCCCCEEEECHHHHHHHHCCCEEEECCCCCCCCCCCCHHHH
KILRRGRDLYRMIVIEKDPKFIFGKLLDIDGEEKEGYLLRKTILMDLQDEIQLGDVLIVK
HHHHCCCCEEEEEEEECCCCEEEHEEECCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEE
LREETEHERNLYEVQFLRFESDTKEDLDLMRMLMKYNYSILYPENIVLNLPEEVDESTVD
EHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCEEECCCCEEEECCHHHCHHHHH
DWGSRVDLRNLKCITIDGEYSKDFDDAISFVEEKSRIRFYVHVADVSYYVRLGTDLDEEA
HHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEECEEEEEEECCCCCHHH
YNRATSVYLGSRVVPMLPPDLSENLCSLVAGKNRLAFTVEMEADWKGKITHVKYYKSVIN
HHHHHHEEECCEECCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHH
VAERYTYNRAESEILSGDPGNWIFRMNEFAKALRARRIEDGRVDLNLKENKVVTDSEHNV
HHHHHHCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCE
VEISVQDRLQAHILIEEFMLSANIKIAESIRKKKHPTLYRIHEPMNEEKLESLNAFLQLN
EEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHEEEC
GIKILLKDSSYEAIRAVLKELEGKPAERLFNMFLLRTFMQAYYSGEYLGHWGLGFEDYCH
CEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
FTSPIRRYPDLVCHRVLQQILLGKKPLYGPEEVVTLGLHCSHQERKASDAERDYYKLKAC
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCHHHHCCCHHHHHHHHHHH
RYLEKTGIKEFSATITGCKPHLIFVDLENPSVDACLISSEFTDEGEIRLETDFSFYSKKY
HHHHHCCCHHHHHEEECCCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEECHHHHHHHH
SKIYTLGDKIEVELDRVDYEEIKIFVKMKKFQKKV
HHEEEECCEEEEEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]