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The map label for this gene is dusB [H]

Identifier: 116326862

GI number: 116326862

Start: 13863

End: 14819

Strand: Direct

Name: dusB [H]

Synonym: LBL_0010

Alternate gene names: 116326862

Gene position: 13863-14819 (Clockwise)

Preceding gene: 116326861

Following gene: 116326864

Centisome position: 0.38

GC content: 39.08

Gene sequence:

>957_bases
TTGATCCGAATCGGGAGCGTAATAATTCCGGGGAGAATTTCACTTTCTCCAATGGCGGGGATTTCAGATTCTCCCACTCG
AAGAATTTGTAGAAAATTCGGAGCTGCCTTTTCTTATACGGAATTTGTAAACACGGACGAACTTGTTCATAAAGCGCCTA
AAGCATTTAAAATGTTTCGTTTTCATCCCGAGGAACGTCCGATTACATTTCAAATTTTTGGAAATCGTTTGGAGATCATT
TCAGAAGCGACCGAAATCATCCAAGAATTAGAACCGGACATCATAGATTTAAATATGGGTTGTTCCACTCGTAAAGTTTC
CTTACGAGGTGCAGGCGCCGGACTTTTACGTAGGCCTTTGTTAGCCGGAAAGATTATAGAAGCTATGAAAAAACGAGTGA
ATATTCCCGTCACAGCAAAAATAAGGATCGGATGGGATGCAACTTCTCGCAACTATCTTGAAGTTTCAAAAATTTTAGAA
GAATCGGGTATAGATGCTCTCACGGTTCACGGTCGTACAAAGGAAATGGCTTATACGGGGTTTGCGGATTGGGATGCAAT
CGGAGAAGTAAAAGTAAATCGGAAAATTCCGATTTTTGGAAATGGGGACATTAAGAGTTTTCAGGAAGCGAATTCTAAAA
TCAACAAATACTCGTTAGACGGAGTTTTGATCGGTAGAAACGCGATCGGAAATCCTTGGATTTTTTCCGAAGTTAAAAAG
GAAGAACTATCTTGGTCTGAGATTTCGGATATAATTTTGCAACACGCACGTTGGATGATTGAGGATTATGGAGAAGAATT
CGGGCTTCTACTTTTCAGAAAACATCTCGTAAAATATTTAAACGGTCTTGAATTTGATCCCACATGGAAGATGGAATTGT
TGGAAATCAAAGAATGGAATCGTTTTGAAGATATTCTTTTATCGGATAAAAGACGAAATGTTTTAGCGGTTCCATAA

Upstream 100 bases:

>100_bases
CATTCGATATTTCTAAACTAGGCAGAACCGCAGTCGGAGTTCGTATTGTGGATTTAAAAGATAACGATAAGGTACAGGAT
TTTACTGTTTTGGGAGAAAA

Downstream 100 bases:

>100_bases
ACCTTCTCCATTATTTCTTGACTAAATCTTCTGTGAACTTGAAAACTTCGAGAAACGAACGGAGAAACCATGTATGAAAA
GTATAAAATTATTTTTAATT

Product: tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MIRIGSVIIPGRISLSPMAGISDSPTRRICRKFGAAFSYTEFVNTDELVHKAPKAFKMFRFHPEERPITFQIFGNRLEII
SEATEIIQELEPDIIDLNMGCSTRKVSLRGAGAGLLRRPLLAGKIIEAMKKRVNIPVTAKIRIGWDATSRNYLEVSKILE
ESGIDALTVHGRTKEMAYTGFADWDAIGEVKVNRKIPIFGNGDIKSFQEANSKINKYSLDGVLIGRNAIGNPWIFSEVKK
EELSWSEISDIILQHARWMIEDYGEEFGLLLFRKHLVKYLNGLEFDPTWKMELLEIKEWNRFEDILLSDKRRNVLAVP

Sequences:

>Translated_318_residues
MIRIGSVIIPGRISLSPMAGISDSPTRRICRKFGAAFSYTEFVNTDELVHKAPKAFKMFRFHPEERPITFQIFGNRLEII
SEATEIIQELEPDIIDLNMGCSTRKVSLRGAGAGLLRRPLLAGKIIEAMKKRVNIPVTAKIRIGWDATSRNYLEVSKILE
ESGIDALTVHGRTKEMAYTGFADWDAIGEVKVNRKIPIFGNGDIKSFQEANSKINKYSLDGVLIGRNAIGNPWIFSEVKK
EELSWSEISDIILQHARWMIEDYGEEFGLLLFRKHLVKYLNGLEFDPTWKMELLEIKEWNRFEDILLSDKRRNVLAVP
>Mature_318_residues
MIRIGSVIIPGRISLSPMAGISDSPTRRICRKFGAAFSYTEFVNTDELVHKAPKAFKMFRFHPEERPITFQIFGNRLEII
SEATEIIQELEPDIIDLNMGCSTRKVSLRGAGAGLLRRPLLAGKIIEAMKKRVNIPVTAKIRIGWDATSRNYLEVSKILE
ESGIDALTVHGRTKEMAYTGFADWDAIGEVKVNRKIPIFGNGDIKSFQEANSKINKYSLDGVLIGRNAIGNPWIFSEVKK
EELSWSEISDIILQHARWMIEDYGEEFGLLLFRKHLVKYLNGLEFDPTWKMELLEIKEWNRFEDILLSDKRRNVLAVP

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=281, Percent_Identity=30.2491103202847, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI239788462, Length=281, Percent_Identity=30.2491103202847, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI40807366, Length=298, Percent_Identity=29.5302013422819, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI31742496, Length=261, Percent_Identity=27.5862068965517, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI8923374, Length=257, Percent_Identity=27.6264591439689, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1789660, Length=289, Percent_Identity=34.9480968858132, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1788462, Length=258, Percent_Identity=31.0077519379845, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI145693211, Length=299, Percent_Identity=27.0903010033445, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=282, Percent_Identity=28.0141843971631, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17507177, Length=254, Percent_Identity=27.9527559055118, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=217, Percent_Identity=28.110599078341, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17510279, Length=299, Percent_Identity=28.0936454849498, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323433, Length=299, Percent_Identity=26.4214046822742, Blast_Score=108, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323560, Length=289, Percent_Identity=26.2975778546713, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=291, Percent_Identity=24.3986254295533, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585320, Length=279, Percent_Identity=30.8243727598566, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24580595, Length=231, Percent_Identity=32.034632034632, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI19920448, Length=231, Percent_Identity=32.034632034632, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=25.6521739130435, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36270; Mature: 36270

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIGSVIIPGRISLSPMAGISDSPTRRICRKFGAAFSYTEFVNTDELVHKAPKAFKMFR
CEEECCEEECCCEECCCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHHCHHHHHHHE
FHPEERPITFQIFGNRLEIISEATEIIQELEPDIIDLNMGCSTRKVSLRGAGAGLLRRPL
ECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCCHHHHHHH
LAGKIIEAMKKRVNIPVTAKIRIGWDATSRNYLEVSKILEESGIDALTVHGRTKEMAYTG
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCHHEEECC
FADWDAIGEVKVNRKIPIFGNGDIKSFQEANSKINKYSLDGVLIGRNAIGNPWIFSEVKK
CCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHCCEEECCEEEEECCCCCCCHHHHHHHH
EELSWSEISDIILQHARWMIEDYGEEFGLLLFRKHLVKYLNGLEFDPTWKMELLEIKEWN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHCC
RFEDILLSDKRRNVLAVP
HHHHHHHCCCCCCEEECC
>Mature Secondary Structure
MIRIGSVIIPGRISLSPMAGISDSPTRRICRKFGAAFSYTEFVNTDELVHKAPKAFKMFR
CEEECCEEECCCEECCCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHHCHHHHHHHE
FHPEERPITFQIFGNRLEIISEATEIIQELEPDIIDLNMGCSTRKVSLRGAGAGLLRRPL
ECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCCHHHHHHH
LAGKIIEAMKKRVNIPVTAKIRIGWDATSRNYLEVSKILEESGIDALTVHGRTKEMAYTG
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCHHEEECC
FADWDAIGEVKVNRKIPIFGNGDIKSFQEANSKINKYSLDGVLIGRNAIGNPWIFSEVKK
CCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHCCEEECCEEEEECCCCCCCHHHHHHHH
EELSWSEISDIILQHARWMIEDYGEEFGLLLFRKHLVKYLNGLEFDPTWKMELLEIKEWN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHCC
RFEDILLSDKRRNVLAVP
HHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]