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The map label for this gene is recF

Identifier: 116326858

GI number: 116326858

Start: 7800

End: 8897

Strand: Direct

Name: recF

Synonym: LBL_0006

Alternate gene names: 116326858

Gene position: 7800-8897 (Clockwise)

Preceding gene: 116326857

Following gene: 116326859

Centisome position: 0.22

GC content: 36.25

Gene sequence:

>1098_bases
ATGTTCTTAAAACATCTTACTCTTCAAAATTTTAGAAGTTATGAAGAACTGAGCTTGGATTTCAATTCCAGGCTTATTTT
TTTTGTAGGCGATAACGGGGAAGGAAAAACGAATCTTCTCGAAGCCATTTGTATGTTATCTTGGTTGAAAAGTTTTCGAG
AGTCCGAGGACTCAAATTTGATTCGATGGGGTTCTGAAAATTATTTCTTGAGAGGGAAAATTAAAGGGGATCAAAAAGAA
TCAGTATTGGAAGTCGGATTTACCGCAAAACCCACGGTTAAAAGGAAATTAAAATTCAATCAAGAGGAAGTAAAAAAAAG
AACGGATCTAATCGGCAAATTCATCACAGTTTTATTAACTCCGATGGATCTAAAAATCATAGAAGGCGGCCCGGCGGAAA
GGAGAAAATTTATCGATGCGTTTATCTCTTCCTTTGATCCGTTTTATCTCGAATGTTTATTGGAATATAATAAAATTTTA
AAGCATAGGAATGCACTTTTAAAAACCGGCATTTCCGACGCCTCTCATTTGTCGATTTGGGATAGAAAGCTCATTGAAAA
GGGAGTTTTGATTCTTAATAAAAGAAAAGAAATTGTTTTTGGATTAAACTCCTTCTACCAACCGAATTTAAACAAATTGA
GCGGCGGGAAAGACGAACTTGAAATGATTTACGGACCGAATGTGAAAGACAAGGATGAATTTGTGGAAAAACTCGGCCGA
AATTTAGGAAAAGATTTAAGACTCGGTTATACTTCTGTGGGAATCCACAGGGATGATCTTTTTATCGGAGCTGATAAACG
TGACATTACCGAATTCGGGTCACAGGGCCAGAAAAGAAGTACGGTGATTGCTTTGAAAGCTGCAACTTTTAATTACTATA
GAAATGTTTTAGATACCATGCCCGTTTTATTGATTGATGATGTGATCCGGGAATTGGATGTTAAACGAAGAGAATATTTT
GTGGACTTAGTCATAAACGCAGGACAAGCTTTTTTCACGACAACAGACTTGGAAGGGATTCAAGATTATGTCGGTAAACT
TAAGGATCAAAAGCAGATCTTTCTGATTCAACAAGGGAATATCCAATTTGCAAAATGA

Upstream 100 bases:

>100_bases
ACGAAATTAAGATCGAATTTTCCGATTCAAGTTCTCCGGTTATTTTCAAGGATCCGTCCGATCCTGAATTTATCTCCGTG
ATTATGCCGATGAAACTGTA

Downstream 100 bases:

>100_bases
AAGAAGATTTGATTCCATCTAAAAAAATTGAAACCTCCGAATTCAATGTCATATTGAGTCAAATGGGAATCACGGAAGAT
AATCTACGGGAAAGAATTTT

Product: recombinational DNA repair ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MFLKHLTLQNFRSYEELSLDFNSRLIFFVGDNGEGKTNLLEAICMLSWLKSFRESEDSNLIRWGSENYFLRGKIKGDQKE
SVLEVGFTAKPTVKRKLKFNQEEVKKRTDLIGKFITVLLTPMDLKIIEGGPAERRKFIDAFISSFDPFYLECLLEYNKIL
KHRNALLKTGISDASHLSIWDRKLIEKGVLILNKRKEIVFGLNSFYQPNLNKLSGGKDELEMIYGPNVKDKDEFVEKLGR
NLGKDLRLGYTSVGIHRDDLFIGADKRDITEFGSQGQKRSTVIALKAATFNYYRNVLDTMPVLLIDDVIRELDVKRREYF
VDLVINAGQAFFTTTDLEGIQDYVGKLKDQKQIFLIQQGNIQFAK

Sequences:

>Translated_365_residues
MFLKHLTLQNFRSYEELSLDFNSRLIFFVGDNGEGKTNLLEAICMLSWLKSFRESEDSNLIRWGSENYFLRGKIKGDQKE
SVLEVGFTAKPTVKRKLKFNQEEVKKRTDLIGKFITVLLTPMDLKIIEGGPAERRKFIDAFISSFDPFYLECLLEYNKIL
KHRNALLKTGISDASHLSIWDRKLIEKGVLILNKRKEIVFGLNSFYQPNLNKLSGGKDELEMIYGPNVKDKDEFVEKLGR
NLGKDLRLGYTSVGIHRDDLFIGADKRDITEFGSQGQKRSTVIALKAATFNYYRNVLDTMPVLLIDDVIRELDVKRREYF
VDLVINAGQAFFTTTDLEGIQDYVGKLKDQKQIFLIQQGNIQFAK
>Mature_365_residues
MFLKHLTLQNFRSYEELSLDFNSRLIFFVGDNGEGKTNLLEAICMLSWLKSFRESEDSNLIRWGSENYFLRGKIKGDQKE
SVLEVGFTAKPTVKRKLKFNQEEVKKRTDLIGKFITVLLTPMDLKIIEGGPAERRKFIDAFISSFDPFYLECLLEYNKIL
KHRNALLKTGISDASHLSIWDRKLIEKGVLILNKRKEIVFGLNSFYQPNLNKLSGGKDELEMIYGPNVKDKDEFVEKLGR
NLGKDLRLGYTSVGIHRDDLFIGADKRDITEFGSQGQKRSTVIALKAATFNYYRNVLDTMPVLLIDDVIRELDVKRREYF
VDLVINAGQAFFTTTDLEGIQDYVGKLKDQKQIFLIQQGNIQFAK

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=362, Percent_Identity=27.6243093922652, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_LEPBJ (Q04WF5)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_799523.1
- STRING:   Q04WF5
- GeneID:   4411211
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0006
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- PhylomeDB:   Q04WF5
- ProtClustDB:   CLSK573281
- BioCyc:   LBOR355277:LBJ_0006-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42084; Mature: 42084

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLKHLTLQNFRSYEELSLDFNSRLIFFVGDNGEGKTNLLEAICMLSWLKSFRESEDSNL
CCCHHHHHHHHCCHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
IRWGSENYFLRGKIKGDQKESVLEVGFTAKPTVKRKLKFNQEEVKKRTDLIGKFITVLLT
EEECCCCEEEEEEECCCCHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PMDLKIIEGGPAERRKFIDAFISSFDPFYLECLLEYNKILKHRNALLKTGISDASHLSIW
CCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
DRKLIEKGVLILNKRKEIVFGLNSFYQPNLNKLSGGKDELEMIYGPNVKDKDEFVEKLGR
HHHHHHCCCEEEECCHHEEEECHHHCCCCCCCCCCCCHHEEEEECCCCCCHHHHHHHHHH
NLGKDLRLGYTSVGIHRDDLFIGADKRDITEFGSQGQKRSTVIALKAATFNYYRNVLDTM
HCCCHHCCCCEEECEEECCEEEECCCHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHH
PVLLIDDVIRELDVKRREYFVDLVINAGQAFFTTTDLEGIQDYVGKLKDQKQIFLIQQGN
HHHHHHHHHHHHHHHHHHHEEEHEEECCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECC
IQFAK
EEECC
>Mature Secondary Structure
MFLKHLTLQNFRSYEELSLDFNSRLIFFVGDNGEGKTNLLEAICMLSWLKSFRESEDSNL
CCCHHHHHHHHCCHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
IRWGSENYFLRGKIKGDQKESVLEVGFTAKPTVKRKLKFNQEEVKKRTDLIGKFITVLLT
EEECCCCEEEEEEECCCCHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PMDLKIIEGGPAERRKFIDAFISSFDPFYLECLLEYNKILKHRNALLKTGISDASHLSIW
CCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
DRKLIEKGVLILNKRKEIVFGLNSFYQPNLNKLSGGKDELEMIYGPNVKDKDEFVEKLGR
HHHHHHCCCEEEECCHHEEEECHHHCCCCCCCCCCCCHHEEEEECCCCCCHHHHHHHHHH
NLGKDLRLGYTSVGIHRDDLFIGADKRDITEFGSQGQKRSTVIALKAATFNYYRNVLDTM
HCCCHHCCCCEEECEEECCEEEECCCHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHH
PVLLIDDVIRELDVKRREYFVDLVINAGQAFFTTTDLEGIQDYVGKLKDQKQIFLIQQGN
HHHHHHHHHHHHHHHHHHHEEEHEEECCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECC
IQFAK
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA