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The map label for this gene is alkJ [H]

Identifier: 116249426

GI number: 116249426

Start: 832186

End: 833823

Strand: Direct

Name: alkJ [H]

Synonym: pRL120764

Alternate gene names: 116249426

Gene position: 832186-833823 (Clockwise)

Preceding gene: 116249425

Following gene: 116249429

Centisome position: 95.65

GC content: 62.09

Gene sequence:

>1638_bases
GTGAATATGCGTGTGAATGATGCAGCCATTGATGCGGCCATTGATGCAGGAACCTACGATTTCATCATCGTCGGCGCGGG
ATCGGCGGGATGCGTTCTGGCCAACCGGCTGTCGGCCGATCCGAAAAGCCGCGTCCTGCTGCTCGAAGCCGGCGGCAGCG
ACCGCTATCATTGGGTGCATGTGCCGATCGGCTATCTCTACTGCATGGGCAATCCACGCACCGATTGGATGATGAAGACG
GCGGCGGAAGCCGGACTGAACGGTCGGTCACTGCCCTATCCGCGTGGAAAAGTGCTGGGCGGCTGTTCGTCGATCAACGG
CATGATCTACATGCGCGGCCAGGCGGCCGACTACGACGGCTGGCGGCAGGCGGGCAATTCCGGCTGGGGCTGGGACGACG
TGCTGCCCTATTTCCTCAAATCCGAGGACAACTATCGCGGCGAATCGCCGATGCATGGGGCAGGCGGCGAATGGCGGGTG
GAGAAGCAGCGGCTGTCCTGGCCGATCCTCGATGCCTTTCGCGATGCGGCCGAAGAACTCGGTATACCGAAGACCGACGA
TTTCAACGATGGCGACAATGAAGGCTCGGGCTATTTCGAGGTCAACCAGCGCGGTGGCCTGCGTTGGAACACGACGAAGG
CCTTTCTGCGCCCGGCGATGAAGCGGCCCAATCTGCGTGTGCTGACCGGCGCCGAATCCGAGCGGCTGGAATTCGACGGC
AGGATGGTGACCGGCGTGCGGTTCCGGCTGAACGGGCGGAGTCATCTGGCTCGCGCCGGTCGCGAGGTCATCCTGTCTGC
CGGTGCGATCAATTCACCGAAAATCCTGGAGCTTTCCGGGGTCGGTCGACCGGATGTATTGTTCGCTGCCGGACTGGACG
TCGTCCATGAACTTCCGGGTGTCGGCGAAAACCTGCAGGACCATCTGCAAATCCGTACGGTCTTCCGCATCGAGGGCGCA
AAGACGCTGAACCAGCTCTATCACAACCTGTTCACCCGGGCGGGCATGGGGCTTGAATATATGCTGCGCCGGTCCGGGCC
GCTGTCTATGGCGCCGAGCCAGCTCGGTATCTTTGCCAAGAGCGATCCGGCTGTTGCGACCGCCGATCTCGAATATCACG
TGCAGCCCTTGAGCACCGACCGGCTCGGCGAGCCGCTGCACAAATATCCCGCCGTCACCGTCTCCGTCTGCAATCTGCGG
CCGGAAAGCCGGGGGACCGTGCATGTCGGCGGCCCGGACTTTTCCGTGGCGCCGGAAATCCGCCCGAATTATCTTTCGAC
CGTCGGTGACCGGATGGTTGCGACGAAATCGATCTGGCACGCCCGCCGGCTTATGGAGGCCGGTGCGATCGCCAAGTACC
GGCCGCAGGAGATGCTGCCGGGCACAGAATACCAGAGTGACGACGACCTGATCCGTCGCGTCGGCGATATCGCCACAACG
ATCTTCCATCCGGTTGGTACCTGCAAGATGGGCAGCGACATGATGGCGGTTGTCGATCCGCAATTGCGGGTGCATGGGCT
GGCGAAACTGCGGGTGGTCGATGCCTCGATCATGCCGACGATCGTGTCGGGCAATACGAACTCGCCGGTGATCATGATTG
CCGAGAAGGCCGCGGAAAGCATTCTATCAGGGGTGTGA

Upstream 100 bases:

>100_bases
ACGCGCGGCGCTACGCTCTCTATCAGGATATCGCCGAAGCGATGGCACCGCTCTGGCGGCGTCTGACGGCGGTCCCGCCG
GCGGCAGCGGGGGCGGCGGC

Downstream 100 bases:

>100_bases
TGAGCTCTTCGCCGTGATGAACTCCTCGCATTGTCTAAAGCGAATGCGTGGAACATCCTTTTGTCAGAGACAAAATGCTC
AGTTTGCCGCGACTGCGCTG

Product: GMC family oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MNMRVNDAAIDAAIDAGTYDFIIVGAGSAGCVLANRLSADPKSRVLLLEAGGSDRYHWVHVPIGYLYCMGNPRTDWMMKT
AAEAGLNGRSLPYPRGKVLGGCSSINGMIYMRGQAADYDGWRQAGNSGWGWDDVLPYFLKSEDNYRGESPMHGAGGEWRV
EKQRLSWPILDAFRDAAEELGIPKTDDFNDGDNEGSGYFEVNQRGGLRWNTTKAFLRPAMKRPNLRVLTGAESERLEFDG
RMVTGVRFRLNGRSHLARAGREVILSAGAINSPKILELSGVGRPDVLFAAGLDVVHELPGVGENLQDHLQIRTVFRIEGA
KTLNQLYHNLFTRAGMGLEYMLRRSGPLSMAPSQLGIFAKSDPAVATADLEYHVQPLSTDRLGEPLHKYPAVTVSVCNLR
PESRGTVHVGGPDFSVAPEIRPNYLSTVGDRMVATKSIWHARRLMEAGAIAKYRPQEMLPGTEYQSDDDLIRRVGDIATT
IFHPVGTCKMGSDMMAVVDPQLRVHGLAKLRVVDASIMPTIVSGNTNSPVIMIAEKAAESILSGV

Sequences:

>Translated_545_residues
MNMRVNDAAIDAAIDAGTYDFIIVGAGSAGCVLANRLSADPKSRVLLLEAGGSDRYHWVHVPIGYLYCMGNPRTDWMMKT
AAEAGLNGRSLPYPRGKVLGGCSSINGMIYMRGQAADYDGWRQAGNSGWGWDDVLPYFLKSEDNYRGESPMHGAGGEWRV
EKQRLSWPILDAFRDAAEELGIPKTDDFNDGDNEGSGYFEVNQRGGLRWNTTKAFLRPAMKRPNLRVLTGAESERLEFDG
RMVTGVRFRLNGRSHLARAGREVILSAGAINSPKILELSGVGRPDVLFAAGLDVVHELPGVGENLQDHLQIRTVFRIEGA
KTLNQLYHNLFTRAGMGLEYMLRRSGPLSMAPSQLGIFAKSDPAVATADLEYHVQPLSTDRLGEPLHKYPAVTVSVCNLR
PESRGTVHVGGPDFSVAPEIRPNYLSTVGDRMVATKSIWHARRLMEAGAIAKYRPQEMLPGTEYQSDDDLIRRVGDIATT
IFHPVGTCKMGSDMMAVVDPQLRVHGLAKLRVVDASIMPTIVSGNTNSPVIMIAEKAAESILSGV
>Mature_545_residues
MNMRVNDAAIDAAIDAGTYDFIIVGAGSAGCVLANRLSADPKSRVLLLEAGGSDRYHWVHVPIGYLYCMGNPRTDWMMKT
AAEAGLNGRSLPYPRGKVLGGCSSINGMIYMRGQAADYDGWRQAGNSGWGWDDVLPYFLKSEDNYRGESPMHGAGGEWRV
EKQRLSWPILDAFRDAAEELGIPKTDDFNDGDNEGSGYFEVNQRGGLRWNTTKAFLRPAMKRPNLRVLTGAESERLEFDG
RMVTGVRFRLNGRSHLARAGREVILSAGAINSPKILELSGVGRPDVLFAAGLDVVHELPGVGENLQDHLQIRTVFRIEGA
KTLNQLYHNLFTRAGMGLEYMLRRSGPLSMAPSQLGIFAKSDPAVATADLEYHVQPLSTDRLGEPLHKYPAVTVSVCNLR
PESRGTVHVGGPDFSVAPEIRPNYLSTVGDRMVATKSIWHARRLMEAGAIAKYRPQEMLPGTEYQSDDDLIRRVGDIATT
IFHPVGTCKMGSDMMAVVDPQLRVHGLAKLRVVDASIMPTIVSGNTNSPVIMIAEKAAESILSGV

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=532, Percent_Identity=41.9172932330827, Blast_Score=386, Evalue=1e-107,
Organism=Escherichia coli, GI1786503, Length=533, Percent_Identity=40.3377110694184, Blast_Score=340, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17532301, Length=561, Percent_Identity=38.1461675579323, Blast_Score=347, Evalue=7e-96,
Organism=Drosophila melanogaster, GI45551458, Length=566, Percent_Identity=37.2791519434629, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI45549471, Length=566, Percent_Identity=37.2791519434629, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI24642055, Length=574, Percent_Identity=36.7595818815331, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24642048, Length=568, Percent_Identity=36.9718309859155, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24642042, Length=564, Percent_Identity=37.4113475177305, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24642059, Length=570, Percent_Identity=35.9649122807018, Blast_Score=287, Evalue=2e-77,
Organism=Drosophila melanogaster, GI17137792, Length=555, Percent_Identity=36.5765765765766, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24642039, Length=562, Percent_Identity=35.5871886120996, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24650267, Length=573, Percent_Identity=34.9040139616056, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24642051, Length=558, Percent_Identity=33.6917562724014, Blast_Score=256, Evalue=4e-68,
Organism=Drosophila melanogaster, GI18859995, Length=561, Percent_Identity=34.9376114081996, Blast_Score=253, Evalue=3e-67,
Organism=Drosophila melanogaster, GI18859993, Length=567, Percent_Identity=33.6860670194003, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24642037, Length=569, Percent_Identity=34.0949033391916, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24642035, Length=306, Percent_Identity=40.5228758169935, Blast_Score=214, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24645930, Length=581, Percent_Identity=28.7435456110155, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24642057, Length=583, Percent_Identity=27.7873070325901, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 59569; Mature: 59569

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMRVNDAAIDAAIDAGTYDFIIVGAGSAGCVLANRLSADPKSRVLLLEAGGSDRYHWVH
CCCCCCHHHHHHEECCCCEEEEEEECCCCCEEEEHHCCCCCCCCEEEEECCCCCCEEEEE
VPIGYLYCMGNPRTDWMMKTAAEAGLNGRSLPYPRGKVLGGCSSINGMIYMRGQAADYDG
EEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCH
WRQAGNSGWGWDDVLPYFLKSEDNYRGESPMHGAGGEWRVEKQRLSWPILDAFRDAAEEL
HHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHC
GIPKTDDFNDGDNEGSGYFEVNQRGGLRWNTTKAFLRPAMKRPNLRVLTGAESERLEFDG
CCCCCCCCCCCCCCCCCEEEECCCCCCEECCHHHHHHHHHCCCCEEEEECCCCCCEEECC
RMVTGVRFRLNGRSHLARAGREVILSAGAINSPKILELSGVGRPDVLFAAGLDVVHELPG
EEEEEEEEEECCHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCEEEEHHHHHHHHCCC
VGENLQDHLQIRTVFRIEGAKTLNQLYHNLFTRAGMGLEYMLRRSGPLSMAPSQLGIFAK
CCCCHHHHHEEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHCEEEEC
SDPAVATADLEYHVQPLSTDRLGEPLHKYPAVTVSVCNLRPESRGTVHVGGPDFSVAPEI
CCCCEEEEEEEEEEEECCCHHCCCHHHHCCCEEEEEEECCCCCCCEEEECCCCCCCCCCC
RPNYLSTVGDRMVATKSIWHARRLMEAGAIAKYRPQEMLPGTEYQSDDDLIRRVGDIATT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHH
IFHPVGTCKMGSDMMAVVDPQLRVHGLAKLRVVDASIMPTIVSGNTNSPVIMIAEKAAES
HHCCCCCCCCCCCEEEEECCCHHEECEEEEEEECCCCCCEEEECCCCCCEEEEEHHHHHH
ILSGV
HHHCC
>Mature Secondary Structure
MNMRVNDAAIDAAIDAGTYDFIIVGAGSAGCVLANRLSADPKSRVLLLEAGGSDRYHWVH
CCCCCCHHHHHHEECCCCEEEEEEECCCCCEEEEHHCCCCCCCCEEEEECCCCCCEEEEE
VPIGYLYCMGNPRTDWMMKTAAEAGLNGRSLPYPRGKVLGGCSSINGMIYMRGQAADYDG
EEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCH
WRQAGNSGWGWDDVLPYFLKSEDNYRGESPMHGAGGEWRVEKQRLSWPILDAFRDAAEEL
HHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHC
GIPKTDDFNDGDNEGSGYFEVNQRGGLRWNTTKAFLRPAMKRPNLRVLTGAESERLEFDG
CCCCCCCCCCCCCCCCCEEEECCCCCCEECCHHHHHHHHHCCCCEEEEECCCCCCEEECC
RMVTGVRFRLNGRSHLARAGREVILSAGAINSPKILELSGVGRPDVLFAAGLDVVHELPG
EEEEEEEEEECCHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCEEEEHHHHHHHHCCC
VGENLQDHLQIRTVFRIEGAKTLNQLYHNLFTRAGMGLEYMLRRSGPLSMAPSQLGIFAK
CCCCHHHHHEEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHCEEEEC
SDPAVATADLEYHVQPLSTDRLGEPLHKYPAVTVSVCNLRPESRGTVHVGGPDFSVAPEI
CCCCEEEEEEEEEEEECCCHHCCCHHHHCCCEEEEEEECCCCCCCEEEECCCCCCCCCCC
RPNYLSTVGDRMVATKSIWHARRLMEAGAIAKYRPQEMLPGTEYQSDDDLIRRVGDIATT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHH
IFHPVGTCKMGSDMMAVVDPQLRVHGLAKLRVVDASIMPTIVSGNTNSPVIMIAEKAAES
HHCCCCCCCCCCCEEEEECCCHHEECEEEEEEECCCCCCEEEECCCCCCEEEEEHHHHHH
ILSGV
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]