BacMap

Definition	Rhizobium leguminosarum bv. viciae 3841 plasmid pRL12, complete sequence.
Accession	NC_008378
Length	870,021

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The map label for this gene is gra-orf6 [H]

Identifier: 116249408

GI number: 116249408

Start: 812962

End: 813768

Strand: Direct

Name: gra-orf6 [H]

Synonym: pRL120746

Alternate gene names: 116249408

Gene position: 812962-813768 (Clockwise)

Preceding gene: 116249407

Following gene: 116249409

Centisome position: 93.44

GC content: 63.07

Gene sequence:

>807_bases
ATGAAAATCGTTGGAGCCGCCATCATCGTCACGGGCTCGGCAACGGGCGTCGGGGCGGCATGCGTGAAGCAATTCGCCGA
GAGCGGCGCCCGGGTGGTTATCAACTACAGCCGCAGCAAAAAAGAGGCCGATGAGACCGGCGATATCTGCCGCAGTCTGG
GCGCGGAGGTCGAGATCGTCCAGGCCGACGTTGCCGATGACGCGGCCTGCCGCCGGATGGTCGCGATTACCGTCAATCGA
TGGGGGCGGCTCGACGCCTTGGTGAACAATGCCGCGATGACGGTCAAATCAGATCCTTTCGATCTGGAGACATTGTCGGC
CGAAGATTTTCAGAACGTTTTCGGCGTCAACGTCATCGGCGCTTATCAGATGTGCCGGGCGGCGGTACCTGCGATGCGGG
ACAGCGGCGGCGGTGCGATCGTCAATGTCTCATCCAACGTTGCCTTTACCGGCGGCGGCAGCTCGCTTGCCTATACGGCC
TCCAAGGGTGCGCTGAATGCGCTGACTTTGGCCTTGGCGCGCACCTGCGGTCCCGATATCCGCGTGAATGCCGTTTGTCC
TGGCATCATCGATACGCGCTGGATGCGCGACACGCTCGGGCCGGATGCCTATGGAGCCATTGCCAAGCGATTTTCGGAAA
CCGCGCCGCTCGGCCGGGTCGCGACACCGGAAGATGTTGCCGGCGCCATTGTCTGGCTTGTTCAGGGGGCAGACTTCGTC
ACCGGCGAATTGCTTTCCGTTGACGGCGGCATCCGCCTGTCCGGTGGCGCTCGCAAACCCGCGGCATCCGGGGGAGCCGC
ATCGTGA

Upstream 100 bases:

>100_bases
ATGTTCTCGACCGCCTGCCGAAGGGCATCGCGGTGTCGGACCAGCTGAAGCAGATGGATGCGCGCCTGTTTTCGACCCAT
GCCATGAAGGACTTTGCCCC

Downstream 100 bases:

>100_bases
CGCTGTCGGCCCAATCCATCCTCGACTTTCCGGTGCCGCAAGCGACACATGTGGTTACCGCCAGGGATGCCATCCTTTAC
GCCCTCTCGACTGGCTACGG

Product: putative short-chain dehydrogenase/oxidoreductase

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: ORF6 [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKIVGAAIIVTGSATGVGAACVKQFAESGARVVINYSRSKKEADETGDICRSLGAEVEIVQADVADDAACRRMVAITVNR
WGRLDALVNNAAMTVKSDPFDLETLSAEDFQNVFGVNVIGAYQMCRAAVPAMRDSGGGAIVNVSSNVAFTGGGSSLAYTA
SKGALNALTLALARTCGPDIRVNAVCPGIIDTRWMRDTLGPDAYGAIAKRFSETAPLGRVATPEDVAGAIVWLVQGADFV
TGELLSVDGGIRLSGGARKPAASGGAAS

Sequences:

>Translated_268_residues
MKIVGAAIIVTGSATGVGAACVKQFAESGARVVINYSRSKKEADETGDICRSLGAEVEIVQADVADDAACRRMVAITVNR
WGRLDALVNNAAMTVKSDPFDLETLSAEDFQNVFGVNVIGAYQMCRAAVPAMRDSGGGAIVNVSSNVAFTGGGSSLAYTA
SKGALNALTLALARTCGPDIRVNAVCPGIIDTRWMRDTLGPDAYGAIAKRFSETAPLGRVATPEDVAGAIVWLVQGADFV
TGELLSVDGGIRLSGGARKPAASGGAAS
>Mature_268_residues
MKIVGAAIIVTGSATGVGAACVKQFAESGARVVINYSRSKKEADETGDICRSLGAEVEIVQADVADDAACRRMVAITVNR
WGRLDALVNNAAMTVKSDPFDLETLSAEDFQNVFGVNVIGAYQMCRAAVPAMRDSGGGAIVNVSSNVAFTGGGSSLAYTA
SKGALNALTLALARTCGPDIRVNAVCPGIIDTRWMRDTLGPDAYGAIAKRFSETAPLGRVATPEDVAGAIVWLVQGADFV
TGELLSVDGGIRLSGGARKPAASGGAAS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI59889578, Length=243, Percent_Identity=35.8024691358025, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI19923817, Length=264, Percent_Identity=31.4393939393939, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI32483357, Length=246, Percent_Identity=34.1463414634146, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI4758504, Length=264, Percent_Identity=29.5454545454545, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI40254992, Length=245, Percent_Identity=30.6122448979592, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI83715985, Length=263, Percent_Identity=28.1368821292776, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI7705925, Length=252, Percent_Identity=34.1269841269841, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI5031737, Length=245, Percent_Identity=32.2448979591837, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI15277342, Length=260, Percent_Identity=29.2307692307692, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI10190704, Length=257, Percent_Identity=28.7937743190661, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI142976729, Length=206, Percent_Identity=27.1844660194175, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI126723191, Length=180, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI126723750, Length=249, Percent_Identity=28.1124497991968, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI210032110, Length=199, Percent_Identity=25.1256281407035, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI66933014, Length=259, Percent_Identity=28.957528957529, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI7706318, Length=208, Percent_Identity=25.9615384615385, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI33667109, Length=187, Percent_Identity=33.1550802139037, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI31542939, Length=204, Percent_Identity=25.9803921568627, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI7657478, Length=194, Percent_Identity=29.8969072164948, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI40254986, Length=195, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1788459, Length=258, Percent_Identity=33.7209302325581, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI2367175, Length=248, Percent_Identity=35.0806451612903, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786812, Length=265, Percent_Identity=33.2075471698113, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI87082160, Length=252, Percent_Identity=30.5555555555556, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787905, Length=252, Percent_Identity=27.7777777777778, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI2367365, Length=249, Percent_Identity=31.7269076305221, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789208, Length=250, Percent_Identity=30.4, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789378, Length=250, Percent_Identity=28.8, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787335, Length=255, Percent_Identity=29.4117647058824, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790717, Length=257, Percent_Identity=28.0155642023346, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1787820, Length=231, Percent_Identity=29.4372294372294, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=28.3524904214559, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788891, Length=241, Percent_Identity=32.3651452282158, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787526, Length=229, Percent_Identity=25.3275109170306, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=250, Percent_Identity=33.2, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17561402, Length=256, Percent_Identity=32.03125, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17560676, Length=248, Percent_Identity=30.241935483871, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17538182, Length=260, Percent_Identity=30, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71994604, Length=249, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17562910, Length=261, Percent_Identity=28.735632183908, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17551412, Length=271, Percent_Identity=29.8892988929889, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560332, Length=273, Percent_Identity=29.3040293040293, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI193204405, Length=278, Percent_Identity=31.294964028777, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17559104, Length=262, Percent_Identity=29.7709923664122, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17562904, Length=270, Percent_Identity=30, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17560150, Length=266, Percent_Identity=29.3233082706767, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17544670, Length=267, Percent_Identity=27.3408239700375, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17563726, Length=262, Percent_Identity=30.5343511450382, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI72000259, Length=268, Percent_Identity=29.1044776119403, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17562990, Length=268, Percent_Identity=29.8507462686567, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17531453, Length=262, Percent_Identity=30.1526717557252, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17555706, Length=247, Percent_Identity=31.1740890688259, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560220, Length=271, Percent_Identity=29.8892988929889, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536651, Length=250, Percent_Identity=27.2, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=29.718875502008, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17565030, Length=266, Percent_Identity=29.6992481203008, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115534694, Length=248, Percent_Identity=28.2258064516129, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=274, Percent_Identity=27.007299270073, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17562908, Length=270, Percent_Identity=28.1481481481481, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536025, Length=252, Percent_Identity=26.1904761904762, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538480, Length=268, Percent_Identity=26.4925373134328, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538486, Length=269, Percent_Identity=26.3940520446097, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17568967, Length=196, Percent_Identity=29.5918367346939, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508651, Length=255, Percent_Identity=24.3137254901961, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=203, Percent_Identity=26.1083743842365, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322227, Length=241, Percent_Identity=28.2157676348548, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324126, Length=222, Percent_Identity=27.4774774774775, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323882, Length=236, Percent_Identity=24.5762711864407, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322226, Length=215, Percent_Identity=27.906976744186, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI23397609, Length=259, Percent_Identity=33.976833976834, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI28571526, Length=247, Percent_Identity=35.6275303643725, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21355319, Length=256, Percent_Identity=32.03125, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24643142, Length=256, Percent_Identity=31.640625, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24644339, Length=256, Percent_Identity=32.421875, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21357041, Length=247, Percent_Identity=34.8178137651822, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI17737361, Length=261, Percent_Identity=29.5019157088123, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24639444, Length=255, Percent_Identity=29.8039215686275, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24644337, Length=205, Percent_Identity=32.1951219512195, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI18859837, Length=227, Percent_Identity=30.8370044052863, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116007236, Length=254, Percent_Identity=25.1968503937008, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21358495, Length=199, Percent_Identity=25.1256281407035, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 27416; Mature: 27416

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00061 ADH_SHORT ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVGAAIIVTGSATGVGAACVKQFAESGARVVINYSRSKKEADETGDICRSLGAEVEIV
CEEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEE
QADVADDAACRRMVAITVNRWGRLDALVNNAAMTVKSDPFDLETLSAEDFQNVFGVNVIG
EECCCCHHHHHEEEEEEECCCCHHHHHHCCCEEEEECCCCCCEECCHHHHHHHHCCHHHH
AYQMCRAAVPAMRDSGGGAIVNVSSNVAFTGGGSSLAYTASKGALNALTLALARTCGPDI
HHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCE
RVNAVCPGIIDTRWMRDTLGPDAYGAIAKRFSETAPLGRVATPEDVAGAIVWLVQGADFV
EEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHEEEEECCCCCC
TGELLSVDGGIRLSGGARKPAASGGAAS
CCEEEEECCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIVGAAIIVTGSATGVGAACVKQFAESGARVVINYSRSKKEADETGDICRSLGAEVEIV
CEEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEE
QADVADDAACRRMVAITVNRWGRLDALVNNAAMTVKSDPFDLETLSAEDFQNVFGVNVIG
EECCCCHHHHHEEEEEEECCCCHHHHHHCCCEEEEECCCCCCEECCHHHHHHHHCCHHHH
AYQMCRAAVPAMRDSGGGAIVNVSSNVAFTGGGSSLAYTASKGALNALTLALARTCGPDI
HHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCE
RVNAVCPGIIDTRWMRDTLGPDAYGAIAKRFSETAPLGRVATPEDVAGAIVWLVQGADFV
EEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHEEEEECCCCCC
TGELLSVDGGIRLSGGARKPAASGGAAS
CCEEEEECCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2583128; 9831526 [H]