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The map label for this gene is fliY [H]

Identifier: 116249395

GI number: 116249395

Start: 798656

End: 799483

Strand: Direct

Name: fliY [H]

Synonym: pRL120733

Alternate gene names: 116249395

Gene position: 798656-799483 (Clockwise)

Preceding gene: 116249392

Following gene: 116249396

Centisome position: 91.8

GC content: 57.73

Gene sequence:

>828_bases
ATGAAGCCTTTTCTATTTTTTCCGGCCATCATGGCACCGTTGCGGGCCGGCCTTGTCACATGCCTTCTTGCGATGGGCGC
AGGTTCTGCGGCCGCCGTCGACAATCCCTATAACCTGATCGAGCCCGGTACTATCAGCGTCGGCACGATGGGCGATTCCA
AGCCCTATACTTTTGCGACCGCGGACGGCCAGTTCACCGGTTTCGATATCGAGCTGTTTCTTAACGTCGTCTCCCGCCTC
GGCTTTCAGAAAGACAAGGTGACGTTTACGGGTCAGGAATTTTCAGCACTCCTGCCGTCGGTCGCAAACGAAAGGTTCGA
CGTTGCCGTTGCGGCGATCGGAACCACCGAAGCCCGCAAGAAGACCATCGACTTTTCCGACGGCTATCTTGCCGGTTATC
TCTCCGTCCTGACCCCGGATGCCGGTATAAAGGATGCAGATGGCCTCAAGGGCAAGCGTCTCGGCGTCGTGCAGGGGACC
TTGCAGGAGGTCTATGCAGCCAAGAATTTCGGGGGAACCGATCTGGTGAAATTTCCCGACAACAATTCCGCGGTGGCCGC
CCTCAACAACGGAACGGTCGATGCGCATTTTCTCGACTACGAGGCCGCCAAGCAATATGGCGAGCGCTATCCCGCGCTGA
AGGTTGCCGTGAATATCCCGTCCTTCGATGCGCCTGCTGGCTTCGTGGTCCGAAAAGGAAATGACGCCTTTCGCACGGCG
CTCAACGGCGCGCTTCACGATGCGATGCAGGACGGCACCTGGAAGACCCTTTACGAAATGTGGTTCCCCGGCTCGCCGAT
GCCGGAACAGTATCTTCCCAAGAAGTGA

Upstream 100 bases:

>100_bases
GAGACCGGTATCTTGTCCATAAAACTCTACCGCACTGCGCGGCAGGGCAGGACGACCGACAATGCCGACAAGAGCATTCC
TTTCAGAGCGGAGAACGACG

Downstream 100 bases:

>100_bases
CGCCGCAAGCCGCCCGCTCATGGCGGGCGGCTTACCAAGGCTCTGCCGGACGGCGGCGTCTGCGCCGGGCAAGAATTCAT
GGGGATCTGAATGAACTGGC

Product: putative substrate-binding periplasmic protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKPFLFFPAIMAPLRAGLVTCLLAMGAGSAAAVDNPYNLIEPGTISVGTMGDSKPYTFATADGQFTGFDIELFLNVVSRL
GFQKDKVTFTGQEFSALLPSVANERFDVAVAAIGTTEARKKTIDFSDGYLAGYLSVLTPDAGIKDADGLKGKRLGVVQGT
LQEVYAAKNFGGTDLVKFPDNNSAVAALNNGTVDAHFLDYEAAKQYGERYPALKVAVNIPSFDAPAGFVVRKGNDAFRTA
LNGALHDAMQDGTWKTLYEMWFPGSPMPEQYLPKK

Sequences:

>Translated_275_residues
MKPFLFFPAIMAPLRAGLVTCLLAMGAGSAAAVDNPYNLIEPGTISVGTMGDSKPYTFATADGQFTGFDIELFLNVVSRL
GFQKDKVTFTGQEFSALLPSVANERFDVAVAAIGTTEARKKTIDFSDGYLAGYLSVLTPDAGIKDADGLKGKRLGVVQGT
LQEVYAAKNFGGTDLVKFPDNNSAVAALNNGTVDAHFLDYEAAKQYGERYPALKVAVNIPSFDAPAGFVVRKGNDAFRTA
LNGALHDAMQDGTWKTLYEMWFPGSPMPEQYLPKK
>Mature_275_residues
MKPFLFFPAIMAPLRAGLVTCLLAMGAGSAAAVDNPYNLIEPGTISVGTMGDSKPYTFATADGQFTGFDIELFLNVVSRL
GFQKDKVTFTGQEFSALLPSVANERFDVAVAAIGTTEARKKTIDFSDGYLAGYLSVLTPDAGIKDADGLKGKRLGVVQGT
LQEVYAAKNFGGTDLVKFPDNNSAVAALNNGTVDAHFLDYEAAKQYGERYPALKVAVNIPSFDAPAGFVVRKGNDAFRTA
LNGALHDAMQDGTWKTLYEMWFPGSPMPEQYLPKK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=226, Percent_Identity=31.4159292035398, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=29.7872340425532, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788649, Length=232, Percent_Identity=30.1724137931034, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787085, Length=223, Percent_Identity=28.2511210762332, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1787031, Length=219, Percent_Identity=29.2237442922374, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787088, Length=253, Percent_Identity=26.0869565217391, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29492; Mature: 29492

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFLFFPAIMAPLRAGLVTCLLAMGAGSAAAVDNPYNLIEPGTISVGTMGDSKPYTFAT
CCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEEECCCCCCCEEEEE
ADGQFTGFDIELFLNVVSRLGFQKDKVTFTGQEFSALLPSVANERFDVAVAAIGTTEARK
CCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHCCCCEEEEEEEECCCHHHH
KTIDFSDGYLAGYLSVLTPDAGIKDADGLKGKRLGVVQGTLQEVYAAKNFGGTDLVKFPD
EECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEECC
NNSAVAALNNGTVDAHFLDYEAAKQYGERYPALKVAVNIPSFDAPAGFVVRKGNDAFRTA
CCCEEEEECCCCEEEEEECHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEECCCHHHHHH
LNGALHDAMQDGTWKTLYEMWFPGSPMPEQYLPKK
HHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHCCCC
>Mature Secondary Structure
MKPFLFFPAIMAPLRAGLVTCLLAMGAGSAAAVDNPYNLIEPGTISVGTMGDSKPYTFAT
CCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEEECCCCCCCEEEEE
ADGQFTGFDIELFLNVVSRLGFQKDKVTFTGQEFSALLPSVANERFDVAVAAIGTTEARK
CCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHCCCCEEEEEEEECCCHHHH
KTIDFSDGYLAGYLSVLTPDAGIKDADGLKGKRLGVVQGTLQEVYAAKNFGGTDLVKFPD
EECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEECC
NNSAVAALNNGTVDAHFLDYEAAKQYGERYPALKVAVNIPSFDAPAGFVVRKGNDAFRTA
CCCEEEEECCCCEEEEEECHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEECCCHHHHHH
LNGALHDAMQDGTWKTLYEMWFPGSPMPEQYLPKK
HHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]