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The map label for this gene is treZ [H]

Identifier: 116249384

GI number: 116249384

Start: 789255

End: 791024

Strand: Direct

Name: treZ [H]

Synonym: pRL120722

Alternate gene names: 116249384

Gene position: 789255-791024 (Clockwise)

Preceding gene: 116249381

Following gene: 116249385

Centisome position: 90.72

GC content: 62.54

Gene sequence:

>1770_bases
TTGACCCAAATGACGTTCGGACCGGCATTCACCGAAGAGGGTGTTCTATTCCGCCTCTGGGCTCCCCTGCATGAAAGCGT
GTCGTTGACGATCGAATGCGCTGATCCGCGGCCGATGCAGGCGGTCGAGGACGGCTGGCACCACTATACGGTCGCGGATG
CCAGTGTCGGTAAGCGCTACCGCTTCGTCCTGCCGGACGGTCTCGAAATACCAGATCCCGCCTCGCGGTTTCAGCCGCAG
GATGTGCACGGGCCGAGCGAAGTGGTCGACCTTTCCTTCTATCGCTGGAAGACGAGCGACTGGACGGGGCGCCCTTGGGA
AGAGATGGTGATCTACGAGATGCATATCGGCAGCTTCACGCCGGAGGGTACTTTCAAGGCCGCGATCGAGCGGCTCGACC
ATCTGCAGGCGCTGGGCGTTACGGCGCTGCAGATCATGCCGCTGAGTGAATTTCCCGGCCGTTACAGCTGGGGTTATGAC
GGCGTGCTTCCCTATGCTCCGGACAGCAGCTACGGCCGGCCGGAAGATTTTATGGCGCTGGTGGACGCAGCGCACCAGCG
CGGCATCTCGGTTTTCCTGGACGTGGTCTACAATCACTTCGGGCCTGACGGGAACTATATCCCTGCCTATGCGCCGCTCT
TTACCGACCACCACAAGACGCCCTGGGGCAACGGCATCAACTACGATGGCGACGGATCGGAGATGATCCGCGAGTTCATC
ATCGAAAACGCCATCTACTGGATCACCGAGTTCAGGCTCGACGGTTTCCGCTTCGATGCCGTCCACGCCATCAAGGACGA
CAGCGCCGAGCATCTGCTTCACGCGCTTGCCCGCCGCGTCAGGGCCGCGGCCGGCGACCGGCATGTTCATCTGATCGTCG
AAAACGAGGAGAACGACAGCGACCTGTTGACGCGTGACGAAAACGGGGAAGTGAAGCTGTTCACCGCTCAGTGGAACGAC
GACGTGCACCATGTGCTGCATATCACTGCCACCGGCGAAACCTTCGGCTATTATGCCGATTACGCCGGTGACGCCGGCAA
GCTCGGCCGGGCGCTGGCGGAAGGTTTCGTGTTTCAGGGGGAACATATGCCGTACCGCGGCGGAAGCCGCGGCAGGCCGA
GCGGCCATCTGCCGCCCACCGCTTTCATCTCCTTCATCCAGAACCATGACCAGATCGGCAACAGGGCGCTGGGGGATCGG
GTTCTGGCTTCGAGCCCGGCTGATGTCGTTAGGGCCGTCGCCGCCATCTACCTGCTGGCGCCTGAGATCCCGATGCTGTT
CATGGGGGAGGAATGGGGCGCCACAGAACCTTTCCCCTTCTTTTGCGATTTCGACGAGGATCTGAACGAGAAGGTCAGGA
AAGGCCGTCGCGAGGAGCTTTCCCGTCTCCCGGGCTTCGACGCCGACGATCTTCTCGACCCGACAGCGCCATCGACCTTC
GCCGCCGCCAAGCTGGACTGGTCGAAACTCGCCTCTTCCGAGGTCCTTGGTTTTTACAGGACGCTTCTCGACCTCCGGCA
CAGCAGGATCGTGCCTTTGCTGAAAGGCGCTGGCGCCGGGAACGCGGTCTATCGCTCGGCGGGAAGCGCGCTCGCGGTGG
ATTGGACCCTGGCGCAAAACCGGCGTCTTCATCTGCACGCCAACCTCGGCGCCGAGGCGGCGCCGCTCGTCTCGCAGCGG
AGCGATGGCGAGACGATCTTCCGTCTCGGCGGCAGCGATGGCGGCAATCTCGTGCCCTGGACGGTGATCTGGAGCATCAG
CGAGGCGTGA

Upstream 100 bases:

>100_bases
GCCTCGCGACGGGGCCGCTTTGCGCTCTCCGTGCCTGCGAAACTTTTTGCCCTGACAAAAGTTACCCAAATCCCAACCCC
ATTTCATCGAAGGCTTTCCT

Downstream 100 bases:

>100_bases
CGGCGTGCTGACAGGAAGGCGATCGAACGATGAAATCTGCTGAGCTCGACAAGCTCGCCCGCCGGCACGGCATCAGCCTG
ACGAGGCCCAGTCCCGACAA

Product: putative alpha amylase

Products: NA

Alternate protein names: MTHase; 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase; Maltooligosyl trehalose trehalohydrolase [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MTQMTFGPAFTEEGVLFRLWAPLHESVSLTIECADPRPMQAVEDGWHHYTVADASVGKRYRFVLPDGLEIPDPASRFQPQ
DVHGPSEVVDLSFYRWKTSDWTGRPWEEMVIYEMHIGSFTPEGTFKAAIERLDHLQALGVTALQIMPLSEFPGRYSWGYD
GVLPYAPDSSYGRPEDFMALVDAAHQRGISVFLDVVYNHFGPDGNYIPAYAPLFTDHHKTPWGNGINYDGDGSEMIREFI
IENAIYWITEFRLDGFRFDAVHAIKDDSAEHLLHALARRVRAAAGDRHVHLIVENEENDSDLLTRDENGEVKLFTAQWND
DVHHVLHITATGETFGYYADYAGDAGKLGRALAEGFVFQGEHMPYRGGSRGRPSGHLPPTAFISFIQNHDQIGNRALGDR
VLASSPADVVRAVAAIYLLAPEIPMLFMGEEWGATEPFPFFCDFDEDLNEKVRKGRREELSRLPGFDADDLLDPTAPSTF
AAAKLDWSKLASSEVLGFYRTLLDLRHSRIVPLLKGAGAGNAVYRSAGSALAVDWTLAQNRRLHLHANLGAEAAPLVSQR
SDGETIFRLGGSDGGNLVPWTVIWSISEA

Sequences:

>Translated_589_residues
MTQMTFGPAFTEEGVLFRLWAPLHESVSLTIECADPRPMQAVEDGWHHYTVADASVGKRYRFVLPDGLEIPDPASRFQPQ
DVHGPSEVVDLSFYRWKTSDWTGRPWEEMVIYEMHIGSFTPEGTFKAAIERLDHLQALGVTALQIMPLSEFPGRYSWGYD
GVLPYAPDSSYGRPEDFMALVDAAHQRGISVFLDVVYNHFGPDGNYIPAYAPLFTDHHKTPWGNGINYDGDGSEMIREFI
IENAIYWITEFRLDGFRFDAVHAIKDDSAEHLLHALARRVRAAAGDRHVHLIVENEENDSDLLTRDENGEVKLFTAQWND
DVHHVLHITATGETFGYYADYAGDAGKLGRALAEGFVFQGEHMPYRGGSRGRPSGHLPPTAFISFIQNHDQIGNRALGDR
VLASSPADVVRAVAAIYLLAPEIPMLFMGEEWGATEPFPFFCDFDEDLNEKVRKGRREELSRLPGFDADDLLDPTAPSTF
AAAKLDWSKLASSEVLGFYRTLLDLRHSRIVPLLKGAGAGNAVYRSAGSALAVDWTLAQNRRLHLHANLGAEAAPLVSQR
SDGETIFRLGGSDGGNLVPWTVIWSISEA
>Mature_588_residues
TQMTFGPAFTEEGVLFRLWAPLHESVSLTIECADPRPMQAVEDGWHHYTVADASVGKRYRFVLPDGLEIPDPASRFQPQD
VHGPSEVVDLSFYRWKTSDWTGRPWEEMVIYEMHIGSFTPEGTFKAAIERLDHLQALGVTALQIMPLSEFPGRYSWGYDG
VLPYAPDSSYGRPEDFMALVDAAHQRGISVFLDVVYNHFGPDGNYIPAYAPLFTDHHKTPWGNGINYDGDGSEMIREFII
ENAIYWITEFRLDGFRFDAVHAIKDDSAEHLLHALARRVRAAAGDRHVHLIVENEENDSDLLTRDENGEVKLFTAQWNDD
VHHVLHITATGETFGYYADYAGDAGKLGRALAEGFVFQGEHMPYRGGSRGRPSGHLPPTAFISFIQNHDQIGNRALGDRV
LASSPADVVRAVAAIYLLAPEIPMLFMGEEWGATEPFPFFCDFDEDLNEKVRKGRREELSRLPGFDADDLLDPTAPSTFA
AAKLDWSKLASSEVLGFYRTLLDLRHSRIVPLLKGAGAGNAVYRSAGSALAVDWTLAQNRRLHLHANLGAEAAPLVSQRS
DGETIFRLGGSDGGNLVPWTVIWSISEA

Specific function: Catalyzes The Formation Of The Alpha-1,6-Glucosidic Linkages In Glycogen By Scission Of A 1,4-Alpha-Linked Oligosaccharide From Growing Alpha-1,4-Glucan Chains And The Subsequent Attachment Of The Oligosaccharide To The Alpha-1,6 Position. Has A Preferenc

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=168, Percent_Identity=31.547619047619, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1789839, Length=281, Percent_Identity=29.1814946619217, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI2367229, Length=538, Percent_Identity=24.1635687732342, Blast_Score=79, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI32564391, Length=180, Percent_Identity=32.2222222222222, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17554896, Length=178, Percent_Identity=31.4606741573034, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320826, Length=176, Percent_Identity=31.25, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR015156
- InterPro:   IPR012768 [H]

Pfam domain/function: PF09071 Alpha-amyl_C; PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.141 [H]

Molecular weight: Translated: 65560; Mature: 65429

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQMTFGPAFTEEGVLFRLWAPLHESVSLTIECADPRPMQAVEDGWHHYTVADASVGKRY
CCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCEEEEECCCCCCEE
RFVLPDGLEIPDPASRFQPQDVHGPSEVVDLSFYRWKTSDWTGRPWEEMVIYEMHIGSFT
EEECCCCCCCCCCHHHCCCCCCCCCHHHEEEEEEEEECCCCCCCCHHHEEEEEEECCCCC
PEGTFKAAIERLDHLQALGVTALQIMPLSEFPGRYSWGYDGVLPYAPDSSYGRPEDFMAL
CCCHHHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VDAAHQRGISVFLDVVYNHFGPDGNYIPAYAPLFTDHHKTPWGNGINYDGDGSEMIREFI
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHH
IENAIYWITEFRLDGFRFDAVHAIKDDSAEHLLHALARRVRAAAGDRHVHLIVENEENDS
HHHHHEEEEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
DLLTRDENGEVKLFTAQWNDDVHHVLHITATGETFGYYADYAGDAGKLGRALAEGFVFQG
CCEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEECCCCCHHHHHHHHHHCCEECC
EHMPYRGGSRGRPSGHLPPTAFISFIQNHDQIGNRALGDRVLASSPADVVRAVAAIYLLA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCHHHCCCHHHHHHHHHHHHHHC
PEIPMLFMGEEWGATEPFPFFCDFDEDLNEKVRKGRREELSRLPGFDADDLLDPTAPSTF
CCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHCCHHHHHHHCCCCCHHHCCCCCCCCCH
AAAKLDWSKLASSEVLGFYRTLLDLRHSRIVPLLKGAGAGNAVYRSAGSALAVDWTLAQN
HEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHCCCCEEEEEEEECCC
RRLHLHANLGAEAAPLVSQRSDGETIFRLGGSDGGNLVPWTVIWSISEA
CEEEEEECCCCCCCCHHHCCCCCCEEEEECCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
TQMTFGPAFTEEGVLFRLWAPLHESVSLTIECADPRPMQAVEDGWHHYTVADASVGKRY
CCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCEEEEECCCCCCEE
RFVLPDGLEIPDPASRFQPQDVHGPSEVVDLSFYRWKTSDWTGRPWEEMVIYEMHIGSFT
EEECCCCCCCCCCHHHCCCCCCCCCHHHEEEEEEEEECCCCCCCCHHHEEEEEEECCCCC
PEGTFKAAIERLDHLQALGVTALQIMPLSEFPGRYSWGYDGVLPYAPDSSYGRPEDFMAL
CCCHHHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VDAAHQRGISVFLDVVYNHFGPDGNYIPAYAPLFTDHHKTPWGNGINYDGDGSEMIREFI
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHH
IENAIYWITEFRLDGFRFDAVHAIKDDSAEHLLHALARRVRAAAGDRHVHLIVENEENDS
HHHHHEEEEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
DLLTRDENGEVKLFTAQWNDDVHHVLHITATGETFGYYADYAGDAGKLGRALAEGFVFQG
CCEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEECCCCCHHHHHHHHHHCCEECC
EHMPYRGGSRGRPSGHLPPTAFISFIQNHDQIGNRALGDRVLASSPADVVRAVAAIYLLA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCHHHCCCHHHHHHHHHHHHHHC
PEIPMLFMGEEWGATEPFPFFCDFDEDLNEKVRKGRREELSRLPGFDADDLLDPTAPSTF
CCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHCCHHHHHHHCCCCCHHHCCCCCCCCCH
AAAKLDWSKLASSEVLGFYRTLLDLRHSRIVPLLKGAGAGNAVYRSAGSALAVDWTLAQN
HEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHCCCCEEEEEEEECCC
RRLHLHANLGAEAAPLVSQRSDGETIFRLGGSDGGNLVPWTVIWSISEA
CEEEEEECCCCCCCCHHHCCCCCCEEEEECCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726 [H]