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The map label for this gene is groEL [H]

Identifier: 116249307

GI number: 116249307

Start: 696038

End: 697666

Strand: Reverse

Name: groEL [H]

Synonym: pRL120642

Alternate gene names: 116249307

Gene position: 697666-696038 (Counterclockwise)

Preceding gene: 116249308

Following gene: 116249306

Centisome position: 80.19

GC content: 61.88

Gene sequence:

>1629_bases
ATGGCTGCTAAAGAAATCAAATTCAGCACCGAAGCTCGCGAGAAGATGCTGCGTGGCGTCGACATCCTGGCCAACGCCGT
GAAGGCGACCCTCGGCCCGAAAGGCCGCAACGTCGTGATCGAAAGGTCTTTCGGCGCGCCGCGCATCACCAAAGACGGCG
TTTCCGTCGCCAAGGAAATCGAACTCGAAGACAAGTTCGAAAACATGGGCGCCCAGATGGTCCGTGAAGTTGCTTCGAAG
ACCAGCGACGTCGCCGGCGACGGCACCACGACTGCAACGGTGCTGGCCCAGGCGATCGTCAAGGAGGGCGCCAAGGCGGT
GACTTCGGGCATGAACCCGATGGATCTGAAACGCGGCATCGATCTTGCGGTCGGCGCCATCGTCGCGGAACTGAAGGCCA
ACGCCCGCAAGATCTCCAACAATTCCGAGATCGCCCAGGTCGGCACGATCTCCGCCAATGGCGATGCCGAAATCGGCCGT
TTTTTGGCGGAAGCCATGGAAAGGGTCGGCAATGATGGCGTCATCACCGTCGAGGAAGCAAAGACTGCCGAAACCGAACT
CGAAGTCGTCGAAGGCATGCAGTTCGACCGCGGCTATCTCAGCCCCTACTTCGTGACCAATGCCGACAAGATGCGGGTCG
AGTTCGAAGACCCTTATATCCTCATCCACGAGAAGAAGCTCTCGAACCTGCAGTCGATGCTGCCGGTTCTGGAAGCCGTC
GTCCAATCCAGCAAGCCGCTGCTCATCATCGCTGAAGACGTCGAAGGCGAAGCTCTTGCTACCCTCGTCGTCAACAAGCT
TCGCGGTGGCCTGAAAATCGCTGCCGTCAAGGCGCCCGGCTTCGGCGATCGTCGCAAAGCCATGCTCGAAGACATCGCCA
TCCTGACTGCCGGCACCGTCATCTCCGAAGATCTCGGCATCAAGCTCGAATCTGTCACGCTCGACATGCTCGGCCGTGCC
AAGAAGGTCTCGATCGAGAAGGAAAACACGACGATCGTCGATGGGTCAGGTGCCAAGTCCGACATCGAAGGTCGCGTTGC
CCAGATCAGGGCTCAGATCGAAGAAACCACCTCCGACTATGACCGCGAGAAGCTGCAGGAGCGCCTTGCCAAGCTCGCCG
GCGGCGTTGCCGTCATTCGCGTCGGCGGCTCGACGGAAGTCGAAGTGAAGGAAAAGAAGGACCGCGTCGACGACGCGCTT
CATGCAACCCGCGCGGCGGTCGAGGAAGGCATTCTACCGGGCGGCGGCGTGGCGCTGCTGCGCGCCGTCAAGGCGCTCGA
CAATGTCGAGACCGCCAATGGCGACCAGCGCGTCGGCGTCGACATCGTTCGCCGCGCGGTCGAGGCGCCGGCTCGCCAGA
TCGCCGAAAACGCCGGAGCGGAAGGCTCGGTCATCGTCGGCAAGCTGCGCGAGAAAAGCGAGTTCTCCTACGGCTGGAAC
GCGCAGACGGGCGAATATGGCGACCTCTATGCGCAGGGCGTCATCGATCCGGCCAAGGTGGTTCGCACCGCGCTGCAGGA
TGCGGCCTCCATCGCCGGCCTTCTCGTCACGACGGAAGCCATGATCGCCGAGAAACCCAGGAAGGACGCGCCGCCGCCAA
TGCCCGCCGGCCACGGCATGGACTTCTAA

Upstream 100 bases:

>100_bases
CAGAACCGCTCCAACCTTTTATTTCTAACGCAATTCCGAACGGAAAACCGCGTCGCACTTTTCCTGGAATTGCTCTGGCC
GCCTATGAAGGAGTGAGGAC

Downstream 100 bases:

>100_bases
GGCGTTTGCCGGCCCGCCGGCCTCGCTGGGCGGGCCTCATCTGAGGGAATAGAGATGGTTCAAACGATCAAAAACCGATC
GCAGGTGCCGCTGGACTCCC

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 2; Protein Cpn60 2 [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MAAKEIKFSTEAREKMLRGVDILANAVKATLGPKGRNVVIERSFGAPRITKDGVSVAKEIELEDKFENMGAQMVREVASK
TSDVAGDGTTTATVLAQAIVKEGAKAVTSGMNPMDLKRGIDLAVGAIVAELKANARKISNNSEIAQVGTISANGDAEIGR
FLAEAMERVGNDGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNADKMRVEFEDPYILIHEKKLSNLQSMLPVLEAV
VQSSKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTAGTVISEDLGIKLESVTLDMLGRA
KKVSIEKENTTIVDGSGAKSDIEGRVAQIRAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGSTEVEVKEKKDRVDDAL
HATRAAVEEGILPGGGVALLRAVKALDNVETANGDQRVGVDIVRRAVEAPARQIAENAGAEGSVIVGKLREKSEFSYGWN
AQTGEYGDLYAQGVIDPAKVVRTALQDAASIAGLLVTTEAMIAEKPRKDAPPPMPAGHGMDF

Sequences:

>Translated_542_residues
MAAKEIKFSTEAREKMLRGVDILANAVKATLGPKGRNVVIERSFGAPRITKDGVSVAKEIELEDKFENMGAQMVREVASK
TSDVAGDGTTTATVLAQAIVKEGAKAVTSGMNPMDLKRGIDLAVGAIVAELKANARKISNNSEIAQVGTISANGDAEIGR
FLAEAMERVGNDGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNADKMRVEFEDPYILIHEKKLSNLQSMLPVLEAV
VQSSKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTAGTVISEDLGIKLESVTLDMLGRA
KKVSIEKENTTIVDGSGAKSDIEGRVAQIRAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGSTEVEVKEKKDRVDDAL
HATRAAVEEGILPGGGVALLRAVKALDNVETANGDQRVGVDIVRRAVEAPARQIAENAGAEGSVIVGKLREKSEFSYGWN
AQTGEYGDLYAQGVIDPAKVVRTALQDAASIAGLLVTTEAMIAEKPRKDAPPPMPAGHGMDF
>Mature_541_residues
AAKEIKFSTEAREKMLRGVDILANAVKATLGPKGRNVVIERSFGAPRITKDGVSVAKEIELEDKFENMGAQMVREVASKT
SDVAGDGTTTATVLAQAIVKEGAKAVTSGMNPMDLKRGIDLAVGAIVAELKANARKISNNSEIAQVGTISANGDAEIGRF
LAEAMERVGNDGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNADKMRVEFEDPYILIHEKKLSNLQSMLPVLEAVV
QSSKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTAGTVISEDLGIKLESVTLDMLGRAK
KVSIEKENTTIVDGSGAKSDIEGRVAQIRAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGSTEVEVKEKKDRVDDALH
ATRAAVEEGILPGGGVALLRAVKALDNVETANGDQRVGVDIVRRAVEAPARQIAENAGAEGSVIVGKLREKSEFSYGWNA
QTGEYGDLYAQGVIDPAKVVRTALQDAASIAGLLVTTEAMIAEKPRKDAPPPMPAGHGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=531, Percent_Identity=53.2956685499058, Blast_Score=578, Evalue=1e-165,
Organism=Homo sapiens, GI31542947, Length=531, Percent_Identity=53.2956685499058, Blast_Score=578, Evalue=1e-165,
Organism=Homo sapiens, GI5453607, Length=546, Percent_Identity=23.2600732600733, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1790586, Length=530, Percent_Identity=66.0377358490566, Blast_Score=695, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=53.1190926275992, Blast_Score=573, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI193210679, Length=222, Percent_Identity=52.7027027027027, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17564182, Length=543, Percent_Identity=23.0202578268877, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=58.5551330798479, Blast_Score=620, Evalue=1e-178,
Organism=Saccharomyces cerevisiae, GI6322350, Length=545, Percent_Identity=24.5871559633028, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=56.8181818181818, Blast_Score=604, Evalue=1e-173,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=56.8181818181818, Blast_Score=604, Evalue=1e-173,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=52.3809523809524, Blast_Score=562, Evalue=1e-160,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=52.3809523809524, Blast_Score=562, Evalue=1e-160,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=52.3809523809524, Blast_Score=562, Evalue=1e-160,
Organism=Drosophila melanogaster, GI17864606, Length=544, Percent_Identity=45.5882352941176, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24584129, Length=536, Percent_Identity=35.8208955223881, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI19921262, Length=536, Percent_Identity=35.8208955223881, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24645179, Length=505, Percent_Identity=21.5841584158416, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57685; Mature: 57553

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKEIKFSTEAREKMLRGVDILANAVKATLGPKGRNVVIERSFGAPRITKDGVSVAKEI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
ELEDKFENMGAQMVREVASKTSDVAGDGTTTATVLAQAIVKEGAKAVTSGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCH
DLAVGAIVAELKANARKISNNSEIAQVGTISANGDAEIGRFLAEAMERVGNDGVITVEEA
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECC
KTAETELEVVEGMQFDRGYLSPYFVTNADKMRVEFEDPYILIHEKKLSNLQSMLPVLEAV
CCHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHH
VQSSKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTAGTV
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCHH
ISEDLGIKLESVTLDMLGRAKKVSIEKENTTIVDGSGAKSDIEGRVAQIRAQIEETTSDY
HHHHHCCEEEEEHHHHHCCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGSTEVEVKEKKDRVDDALHATRAAVEEGILPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RAVKALDNVETANGDQRVGVDIVRRAVEAPARQIAENAGAEGSVIVGKLREKSEFSYGWN
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHCCCCCCCCC
AQTGEYGDLYAQGVIDPAKVVRTALQDAASIAGLLVTTEAMIAEKPRKDAPPPMPAGHGM
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DF
CC
>Mature Secondary Structure 
AAKEIKFSTEAREKMLRGVDILANAVKATLGPKGRNVVIERSFGAPRITKDGVSVAKEI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
ELEDKFENMGAQMVREVASKTSDVAGDGTTTATVLAQAIVKEGAKAVTSGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCH
DLAVGAIVAELKANARKISNNSEIAQVGTISANGDAEIGRFLAEAMERVGNDGVITVEEA
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECC
KTAETELEVVEGMQFDRGYLSPYFVTNADKMRVEFEDPYILIHEKKLSNLQSMLPVLEAV
CCHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHH
VQSSKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTAGTV
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCHH
ISEDLGIKLESVTLDMLGRAKKVSIEKENTTIVDGSGAKSDIEGRVAQIRAQIEETTSDY
HHHHHCCEEEEEHHHHHCCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGSTEVEVKEKKDRVDDALHATRAAVEEGILPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RAVKALDNVETANGDQRVGVDIVRRAVEAPARQIAENAGAEGSVIVGKLREKSEFSYGWN
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHCCCCCCCCC
AQTGEYGDLYAQGVIDPAKVVRTALQDAASIAGLLVTTEAMIAEKPRKDAPPPMPAGHGM
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA