Click here to switch to the map view.

The map label for this gene is ghrA [H]

Identifier: 116249297

GI number: 116249297

Start: 688872

End: 689795

Strand: Reverse

Name: ghrA [H]

Synonym: pRL120632

Alternate gene names: 116249297

Gene position: 689795-688872 (Counterclockwise)

Preceding gene: 116249298

Following gene: 116249296

Centisome position: 79.28

GC content: 65.58

Gene sequence:

>924_bases
ATGACCTTTCTCTTCAATTCCGATGTGAAGCGCGGCGCCATATTCGCCGAAGCCTTCGCGCGCGAACTGCCCGACATTGC
CTTCTCCATGGATCCCAAGTCGGTCGAACGGGACGCCGTGCGCTACCTGATCACCTGGACCGTGCCGCAGGATCTCGCAC
GCTATCGCAATCTCCAAATCCTGTTTTCGATCGGCGCCGGCATCGACCAGCTCCGTATCGATGCGGTGCCGGCGTCGGTG
AAGGTGGTGCGCATGGTCGAGGACGGCATCATCAGGATGATGCAGGAATATGTGACGCTGGCAGTCCTTGCGCTGCATCG
CGACCTGCCGGCCTATCTCGACCAGCAGCGGCGCCGGACCTGGCAGACGATCGCTCCTAGGCAGGCCTCGGAACGCCGCA
TCGGCGTGCTCGGCCTCGGCATGCTTGGAAGCGCCGTGCTCGAGCGGTTGCAGCCCTTCGGCTTTCCGCTTTCGGGCTGG
AGCCGCAGCCCGCGCGAAATCGACGGCGTCCGATGCCTGAGCGGCCGCGACGGCCTCGATACTCTGCTTGCAACCACCGA
CATTCTCGTCTGCCTGCTGCCGCTGACGGGCGAGACGCGCGGCTTCCTGAATGCCGAGCTTTTTGCCGGACTGCCGGCCG
GCGCCACGCTCGTCCATGTCGGCCGAGGCGCTCAACTCGATCATCAGGCGCTCATTGACGCGCTCGACGCCGGGCATCTC
TCCGGCGCCGTCGTCGACGTCACCGATCCGGAGCCGCTGCCTGCGGACCACGCCTTCTGGAACCATCCGAAGATCCTGCT
GACGCCGCACATTGCGAGCGTCACCCAGCCTGAAACCGCCGCGGCGGCCGTCATCGCCAACATCAAGCGGCACCAGGCCG
GCGTCGATCCGATCGGCCTGGTCGATCGCAACCGCGGCTACTGA

Upstream 100 bases:

>100_bases
GGACGAATTCATCCTCGCCGGCGAACTGCTCGCCTGCCAGGCGATGATCGAGGCACTCGGCCGCCGTTGCGCCTGATGAC
ATGATCGAGGAACCGACGCC

Downstream 100 bases:

>100_bases
GTTCTCAACCTAGGAAAAGCAAAATCATGTCCCTCCTCAAGACGATCGACACCAACCCGACCTTCGCGCCGCGCGAGTCC
GGCCCCCTCCCCGAACGGCT

Product: putative dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTFLFNSDVKRGAIFAEAFARELPDIAFSMDPKSVERDAVRYLITWTVPQDLARYRNLQILFSIGAGIDQLRIDAVPASV
KVVRMVEDGIIRMMQEYVTLAVLALHRDLPAYLDQQRRRTWQTIAPRQASERRIGVLGLGMLGSAVLERLQPFGFPLSGW
SRSPREIDGVRCLSGRDGLDTLLATTDILVCLLPLTGETRGFLNAELFAGLPAGATLVHVGRGAQLDHQALIDALDAGHL
SGAVVDVTDPEPLPADHAFWNHPKILLTPHIASVTQPETAAAAVIANIKRHQAGVDPIGLVDRNRGY

Sequences:

>Translated_307_residues
MTFLFNSDVKRGAIFAEAFARELPDIAFSMDPKSVERDAVRYLITWTVPQDLARYRNLQILFSIGAGIDQLRIDAVPASV
KVVRMVEDGIIRMMQEYVTLAVLALHRDLPAYLDQQRRRTWQTIAPRQASERRIGVLGLGMLGSAVLERLQPFGFPLSGW
SRSPREIDGVRCLSGRDGLDTLLATTDILVCLLPLTGETRGFLNAELFAGLPAGATLVHVGRGAQLDHQALIDALDAGHL
SGAVVDVTDPEPLPADHAFWNHPKILLTPHIASVTQPETAAAAVIANIKRHQAGVDPIGLVDRNRGY
>Mature_306_residues
TFLFNSDVKRGAIFAEAFARELPDIAFSMDPKSVERDAVRYLITWTVPQDLARYRNLQILFSIGAGIDQLRIDAVPASVK
VVRMVEDGIIRMMQEYVTLAVLALHRDLPAYLDQQRRRTWQTIAPRQASERRIGVLGLGMLGSAVLERLQPFGFPLSGWS
RSPREIDGVRCLSGRDGLDTLLATTDILVCLLPLTGETRGFLNAELFAGLPAGATLVHVGRGAQLDHQALIDALDAGHLS
GAVVDVTDPEPLPADHAFWNHPKILLTPHIASVTQPETAAAAVIANIKRHQAGVDPIGLVDRNRGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=228, Percent_Identity=28.0701754385965, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI23308577, Length=228, Percent_Identity=28.0701754385965, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI61743967, Length=257, Percent_Identity=26.8482490272374, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI4557497, Length=257, Percent_Identity=26.8482490272374, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI145580578, Length=248, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4557499, Length=248, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI145580575, Length=237, Percent_Identity=26.1603375527426, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI87081824, Length=281, Percent_Identity=36.6548042704626, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI87082289, Length=232, Percent_Identity=28.0172413793103, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789279, Length=225, Percent_Identity=26.2222222222222, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=245, Percent_Identity=28.1632653061224, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25147481, Length=244, Percent_Identity=26.2295081967213, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324055, Length=165, Percent_Identity=31.5151515151515, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324964, Length=268, Percent_Identity=25, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320925, Length=283, Percent_Identity=24.3816254416961, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322116, Length=208, Percent_Identity=26.4423076923077, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28574286, Length=166, Percent_Identity=33.7349397590361, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19921140, Length=262, Percent_Identity=25.5725190839695, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45552429, Length=166, Percent_Identity=34.3373493975904, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI45551003, Length=166, Percent_Identity=34.3373493975904, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28574284, Length=166, Percent_Identity=34.3373493975904, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585514, Length=166, Percent_Identity=34.3373493975904, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28574282, Length=166, Percent_Identity=34.3373493975904, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585516, Length=154, Percent_Identity=33.7662337662338, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571528, Length=159, Percent_Identity=30.188679245283, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24646446, Length=281, Percent_Identity=26.6903914590747, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646448, Length=281, Percent_Identity=26.6903914590747, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646452, Length=281, Percent_Identity=26.6903914590747, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646450, Length=281, Percent_Identity=26.6903914590747, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI62472511, Length=281, Percent_Identity=26.6903914590747, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 33606; Mature: 33475

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLFNSDVKRGAIFAEAFARELPDIAFSMDPKSVERDAVRYLITWTVPQDLARYRNLQI
CEEEECCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHEEEEEEECCHHHHHHHCCEEE
LFSIGAGIDQLRIDAVPASVKVVRMVEDGIIRMMQEYVTLAVLALHRDLPAYLDQQRRRT
EEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
WQTIAPRQASERRIGVLGLGMLGSAVLERLQPFGFPLSGWSRSPREIDGVRCLSGRDGLD
HHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHH
TLLATTDILVCLLPLTGETRGFLNAELFAGLPAGATLVHVGRGAQLDHQALIDALDAGHL
HHHHHHHHHHHEECCCCCCCCCCCHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCC
SGAVVDVTDPEPLPADHAFWNHPKILLTPHIASVTQPETAAAAVIANIKRHQAGVDPIGL
CCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
VDRNRGY
CCCCCCC
>Mature Secondary Structure 
TFLFNSDVKRGAIFAEAFARELPDIAFSMDPKSVERDAVRYLITWTVPQDLARYRNLQI
EEEECCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHEEEEEEECCHHHHHHHCCEEE
LFSIGAGIDQLRIDAVPASVKVVRMVEDGIIRMMQEYVTLAVLALHRDLPAYLDQQRRRT
EEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
WQTIAPRQASERRIGVLGLGMLGSAVLERLQPFGFPLSGWSRSPREIDGVRCLSGRDGLD
HHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHH
TLLATTDILVCLLPLTGETRGFLNAELFAGLPAGATLVHVGRGAQLDHQALIDALDAGHL
HHHHHHHHHHHEECCCCCCCCCCCHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCC
SGAVVDVTDPEPLPADHAFWNHPKILLTPHIASVTQPETAAAAVIANIKRHQAGVDPIGL
CCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
VDRNRGY
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA