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The map label for this gene is dppA [H]

Identifier: 116249275

GI number: 116249275

Start: 662627

End: 664237

Strand: Reverse

Name: dppA [H]

Synonym: pRL120610

Alternate gene names: 116249275

Gene position: 664237-662627 (Counterclockwise)

Preceding gene: 116249276

Following gene: 116249274

Centisome position: 76.35

GC content: 59.4

Gene sequence:

>1611_bases
ATGAACGACAAAATCACCAATTGGACCAGCTCCGACGACGCCATGGTCGAAAGCGCCATTCGTCGTGGCGCCACCCGTCG
TGAATTGCTGCATATGATGCTTGCGGGCGGCGTGGCCATATCAGCCGGCGGGCTCGTGCTTGGCCGTGCCGGCAAGGCGC
TCGCCGCCACGCCCGTTTCCGGCGGCGCGCTCAAAGCGGCCGGCTGGTCGTCCTCCACGGCCGATACGCTCGACCCCGCC
AAGGCGTCGCTCTCCACCGACTATGTCCGGTGCTGCTCCTTCTATAACCGCCTTACCTTCCTCGACAAATCAGGCACGCC
GCAGATGGAGCTTGCCGACGCAATCGAGTCCAAGGATGCGAAGACCTGGACGGTCAAGTTGAAGACCGGCGTCACCTTCC
ACGACGGCAAGCCGCTGACCGCCGATGACGTGGTTTTCTCGCTGAAGCGCCATCTCGACCCATCCGTCGGTTCGAAGGTC
GCCAAGATCGCCGCCCAGATGACCGGGTTCAAGGCCATCGACAAACAGACCGTCGAGATCACGCTCGCCAGCCCGAATGC
CGACCTGCCGACCATTCTGTCGATGCATCATTTCATGATCGTTTCCGACGGCACGACCGATTTCACCAAGGCCAACGGCA
CCGGCGCTTTCGTCAAGGAAGTATTCGAGCCAGGCGTTCGGTCGGTCGGGATCAAGAACAAGAATTACTGGAAATCCGGC
CCGAACGTCGATTCCTTCGAATATTTCGCGATCAGCGACGACAATGCCCGTGTAAACGCACTGCTTGCGGGCGACATCCA
TCTCGCAGCCACGATCAATCCGCGCTCTATGCGCCTCGTCGAGGCCCAGGGCGACGGCTTCACCTTGTCTAAGACGACGT
CCGGCAACTATACCAATCTCAACATGCGACTGGATATGGAGCCCGGCAACAAGCGGGACTTCATCGAGGGCATGAAATAT
CTCGTCAACCGCGAACAGATCGTCAAATCGGCACTGCGCGGTCTCGGTGAAGTCGGCAACGATCAACCCGTTTCGCCGGC
GAACTTCTATCATGACGCAGAGCTGAAAGCGCGAGCCTTCGATCCCGAGAAGGCGAAGTTCCACTTCGAAAAGGCCGGCG
TGCTTGGCCAGTCCATTCCGATCATCGCTTCCGATGCGGCGAGCTCTTCTATCGACATGGCCATGATCATCCAGGCGGCC
GGCGCCGAAATCGGCATGAAGCTCGATGTGCAGCGCGTGCCATCCGATGGTTATTGGGACAATTACTGGCTCAAGGCGCC
GATCCACTTCGGCAATATCAACCCGCGGCCGACACCCGATATCCTCTTCTCCCTGCTCTACACCTCGGACGCTCCGTGGA
ACGAAAGCCACTACAAGTCGGAGAAGTTCGACAAGATGCTGATCGAGGCGCGCGGCTCTCTCGATCAAGACAAGCGCAAG
ACGATCTATAACGAGATGCAGGGCATGGTCGCCCAGGAAGCCGGCACTATCATTCCGGCCTATATCTCGAACGTCGATGC
CACGACCGCCAAGCTCAAGGGCCTGGAAGCCAACCCGCTCGGCGGCCAGATGGGATACGCTTTCGCGGAATATGTCTGGC
TTGAAGCCTGA

Upstream 100 bases:

>100_bases
CGGCCTTGCCGATGCCGTGGCCGCTGCGCGCGCCCGACTTTGAAAGCAGATCGGGCCTGCCCGCAACGGGCAGCCCGCCA
CACAAGAAAAGGGGAATGAG

Downstream 100 bases:

>100_bases
TGCAGCAAAGGAGACTGGGGCTGCAGCCGCGCCCCAGTTCGTCCATCTGCCTTGATGGAACTCAACTCGAACGCAAAGGG
AAGACGCCCGTGAACCGCCA

Product: putative substrate-binding component of ABC transporter

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MNDKITNWTSSDDAMVESAIRRGATRRELLHMMLAGGVAISAGGLVLGRAGKALAATPVSGGALKAAGWSSSTADTLDPA
KASLSTDYVRCCSFYNRLTFLDKSGTPQMELADAIESKDAKTWTVKLKTGVTFHDGKPLTADDVVFSLKRHLDPSVGSKV
AKIAAQMTGFKAIDKQTVEITLASPNADLPTILSMHHFMIVSDGTTDFTKANGTGAFVKEVFEPGVRSVGIKNKNYWKSG
PNVDSFEYFAISDDNARVNALLAGDIHLAATINPRSMRLVEAQGDGFTLSKTTSGNYTNLNMRLDMEPGNKRDFIEGMKY
LVNREQIVKSALRGLGEVGNDQPVSPANFYHDAELKARAFDPEKAKFHFEKAGVLGQSIPIIASDAASSSIDMAMIIQAA
GAEIGMKLDVQRVPSDGYWDNYWLKAPIHFGNINPRPTPDILFSLLYTSDAPWNESHYKSEKFDKMLIEARGSLDQDKRK
TIYNEMQGMVAQEAGTIIPAYISNVDATTAKLKGLEANPLGGQMGYAFAEYVWLEA

Sequences:

>Translated_536_residues
MNDKITNWTSSDDAMVESAIRRGATRRELLHMMLAGGVAISAGGLVLGRAGKALAATPVSGGALKAAGWSSSTADTLDPA
KASLSTDYVRCCSFYNRLTFLDKSGTPQMELADAIESKDAKTWTVKLKTGVTFHDGKPLTADDVVFSLKRHLDPSVGSKV
AKIAAQMTGFKAIDKQTVEITLASPNADLPTILSMHHFMIVSDGTTDFTKANGTGAFVKEVFEPGVRSVGIKNKNYWKSG
PNVDSFEYFAISDDNARVNALLAGDIHLAATINPRSMRLVEAQGDGFTLSKTTSGNYTNLNMRLDMEPGNKRDFIEGMKY
LVNREQIVKSALRGLGEVGNDQPVSPANFYHDAELKARAFDPEKAKFHFEKAGVLGQSIPIIASDAASSSIDMAMIIQAA
GAEIGMKLDVQRVPSDGYWDNYWLKAPIHFGNINPRPTPDILFSLLYTSDAPWNESHYKSEKFDKMLIEARGSLDQDKRK
TIYNEMQGMVAQEAGTIIPAYISNVDATTAKLKGLEANPLGGQMGYAFAEYVWLEA
>Mature_536_residues
MNDKITNWTSSDDAMVESAIRRGATRRELLHMMLAGGVAISAGGLVLGRAGKALAATPVSGGALKAAGWSSSTADTLDPA
KASLSTDYVRCCSFYNRLTFLDKSGTPQMELADAIESKDAKTWTVKLKTGVTFHDGKPLTADDVVFSLKRHLDPSVGSKV
AKIAAQMTGFKAIDKQTVEITLASPNADLPTILSMHHFMIVSDGTTDFTKANGTGAFVKEVFEPGVRSVGIKNKNYWKSG
PNVDSFEYFAISDDNARVNALLAGDIHLAATINPRSMRLVEAQGDGFTLSKTTSGNYTNLNMRLDMEPGNKRDFIEGMKY
LVNREQIVKSALRGLGEVGNDQPVSPANFYHDAELKARAFDPEKAKFHFEKAGVLGQSIPIIASDAASSSIDMAMIIQAA
GAEIGMKLDVQRVPSDGYWDNYWLKAPIHFGNINPRPTPDILFSLLYTSDAPWNESHYKSEKFDKMLIEARGSLDQDKRK
TIYNEMQGMVAQEAGTIIPAYISNVDATTAKLKGLEANPLGGQMGYAFAEYVWLEA

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=498, Percent_Identity=25.1004016064257, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1787052, Length=527, Percent_Identity=25.6166982922201, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787762, Length=482, Percent_Identity=24.6887966804979, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789887, Length=444, Percent_Identity=25.4504504504505, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789397, Length=513, Percent_Identity=22.2222222222222, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787551, Length=528, Percent_Identity=24.0530303030303, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787495, Length=267, Percent_Identity=25.0936329588015, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87081878, Length=227, Percent_Identity=25.5506607929515, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58223; Mature: 58223

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKITNWTSSDDAMVESAIRRGATRRELLHMMLAGGVAISAGGLVLGRAGKALAATPVS
CCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCEEEECCCCEEEECCCC
GGALKAAGWSSSTADTLDPAKASLSTDYVRCCSFYNRLTFLDKSGTPQMELADAIESKDA
CCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCC
KTWTVKLKTGVTFHDGKPLTADDVVFSLKRHLDPSVGSKVAKIAAQMTGFKAIDKQTVEI
EEEEEEEEECEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHCCCEEEEE
TLASPNADLPTILSMHHFMIVSDGTTDFTKANGTGAFVKEVFEPGVRSVGIKNKNYWKSG
EEECCCCCCCHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCHHHCCCCCCCCCCCC
PNVDSFEYFAISDDNARVNALLAGDIHLAATINPRSMRLVEAQGDGFTLSKTTSGNYTNL
CCCCCEEEEEEECCCCEEEEEEECCEEEEEEECCCCEEEEEECCCCEEEEEECCCCEEEE
NMRLDMEPGNKRDFIEGMKYLVNREQIVKSALRGLGEVGNDQPVSPANFYHDAELKARAF
EEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCEEECC
DPEKAKFHFEKAGVLGQSIPIIASDAASSSIDMAMIIQAAGAEIGMKLDVQRVPSDGYWD
CCHHHEEEEHHHCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCC
NYWLKAPIHFGNINPRPTPDILFSLLYTSDAPWNESHYKSEKFDKMLIEARGSLDQDKRK
CEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHH
TIYNEMQGMVAQEAGTIIPAYISNVDATTAKLKGLEANPLGGQMGYAFAEYVWLEA
HHHHHHHHHHHHHCCCEEEHHHCCCCCCHHHHCCCCCCCCCCHHHHHEEHEEEECC
>Mature Secondary Structure
MNDKITNWTSSDDAMVESAIRRGATRRELLHMMLAGGVAISAGGLVLGRAGKALAATPVS
CCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCEEEECCCCEEEECCCC
GGALKAAGWSSSTADTLDPAKASLSTDYVRCCSFYNRLTFLDKSGTPQMELADAIESKDA
CCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCC
KTWTVKLKTGVTFHDGKPLTADDVVFSLKRHLDPSVGSKVAKIAAQMTGFKAIDKQTVEI
EEEEEEEEECEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHCCCEEEEE
TLASPNADLPTILSMHHFMIVSDGTTDFTKANGTGAFVKEVFEPGVRSVGIKNKNYWKSG
EEECCCCCCCHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCHHHCCCCCCCCCCCC
PNVDSFEYFAISDDNARVNALLAGDIHLAATINPRSMRLVEAQGDGFTLSKTTSGNYTNL
CCCCCEEEEEEECCCCEEEEEEECCEEEEEEECCCCEEEEEECCCCEEEEEECCCCEEEE
NMRLDMEPGNKRDFIEGMKYLVNREQIVKSALRGLGEVGNDQPVSPANFYHDAELKARAF
EEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCEEECC
DPEKAKFHFEKAGVLGQSIPIIASDAASSSIDMAMIIQAAGAEIGMKLDVQRVPSDGYWD
CCHHHEEEEHHHCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCC
NYWLKAPIHFGNINPRPTPDILFSLLYTSDAPWNESHYKSEKFDKMLIEARGSLDQDKRK
CEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHH
TIYNEMQGMVAQEAGTIIPAYISNVDATTAKLKGLEANPLGGQMGYAFAEYVWLEA
HHHHHHHHHHHHHCCCEEEHHHCCCCCCHHHHCCCCCCCCCCHHHHHEEHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]