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The map label for this gene is gsiD [H]

Identifier: 116249273

GI number: 116249273

Start: 660724

End: 661590

Strand: Reverse

Name: gsiD [H]

Synonym: pRL120608

Alternate gene names: 116249273

Gene position: 661590-660724 (Counterclockwise)

Preceding gene: 116249274

Following gene: 116249272

Centisome position: 76.04

GC content: 62.28

Gene sequence:

>867_bases
ATGACCATGACCAGTTCTGAAACCACCTCCGGCCGGCTGTCCGGAACCCGGTTTGGCTATCGCTTCAACATCGTCGGCGC
GATCGGCTTCACTGTGATCCTCTTATGGGCGCTCGTCGCGATCTTCGCACCATGGGTCATCCCCTACCCCGTCGGCGAGA
TTATTGATCTCGACTATTTCGGCCCGATGAGCCGGGAACTCTGGCTCGGCTCCGATTATCTCGGCCGCGACATGCTCTCG
CGGATTCTGATGGGCGCGCGCTATACGGTCGGCATCTCGCTGGCGGCGGTCACGATCGCCTGTTTCTGCGGCGTGGTGCT
CGGCATGATCGCAGCGGTCGCCGGCGGCTGGCTGGACACGATCCTCAGCCGCTTTCTCGATGCGCTTAACTCCATCCCGA
GCAAGCTGTTCGGCCTGGTGGTCGTCGCTGCCGTCGGCTCCTCGGTTCCGGTGCTGATCATGACGCTGTCGGTGATCTAC
ATCCCAGGCGCTTACCGCTTCGCCCGGGCGCTCGCCGTCAACATCAACGCGATGGATTTCATCACCGTCGCGCGCATCCG
TGGCGAAAGCACCCTCTATCTCATTCGTTCGGAAATCCTGCCCAACATCGTCGGACCGGTGCTTGCCGATCTCGGCATCC
GCTTCGTCTTCATCGTTCTGCTGCTTTCCGGACTTTCCTTCCTCGGCCTTGGCGTCCAGCCGCCCTACGCCGATTGGGGT
GCGCTCGTGCGCGAGAACATCGGCGGCCTGCCATTCGGTGCGCCGGCGGTGATGTTTCCCTCGCTCGCCATCGCCAGCCT
GACGATCAGCGTCAACCTGCTGATCGACAACCTGCCGCAGAAAATCCGCGACCGGAGTGTGTCATGA

Upstream 100 bases:

>100_bases
TGCCGCTGATCCAGAGCTGCGCGATGATCTTCTGCCTGGGCTATCTGCTGCTGATCACCATCGCCGACATCATCGCCATC
CTTTCCAATCCGAGGCTCCG

Downstream 100 bases:

>100_bases
GCAATTTCATCGAAATCCGTGACCTGAAGGTCGAGGCCACCACCGATTCCGGCCGACGCGTCGAAATCATCAAGGGTGTC
AGCCTCGATGTTGCCGAGGG

Product: putative permease component of ABC transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTMTSSETTSGRLSGTRFGYRFNIVGAIGFTVILLWALVAIFAPWVIPYPVGEIIDLDYFGPMSRELWLGSDYLGRDMLS
RILMGARYTVGISLAAVTIACFCGVVLGMIAAVAGGWLDTILSRFLDALNSIPSKLFGLVVVAAVGSSVPVLIMTLSVIY
IPGAYRFARALAVNINAMDFITVARIRGESTLYLIRSEILPNIVGPVLADLGIRFVFIVLLLSGLSFLGLGVQPPYADWG
ALVRENIGGLPFGAPAVMFPSLAIASLTISVNLLIDNLPQKIRDRSVS

Sequences:

>Translated_288_residues
MTMTSSETTSGRLSGTRFGYRFNIVGAIGFTVILLWALVAIFAPWVIPYPVGEIIDLDYFGPMSRELWLGSDYLGRDMLS
RILMGARYTVGISLAAVTIACFCGVVLGMIAAVAGGWLDTILSRFLDALNSIPSKLFGLVVVAAVGSSVPVLIMTLSVIY
IPGAYRFARALAVNINAMDFITVARIRGESTLYLIRSEILPNIVGPVLADLGIRFVFIVLLLSGLSFLGLGVQPPYADWG
ALVRENIGGLPFGAPAVMFPSLAIASLTISVNLLIDNLPQKIRDRSVS
>Mature_287_residues
TMTSSETTSGRLSGTRFGYRFNIVGAIGFTVILLWALVAIFAPWVIPYPVGEIIDLDYFGPMSRELWLGSDYLGRDMLSR
ILMGARYTVGISLAAVTIACFCGVVLGMIAAVAGGWLDTILSRFLDALNSIPSKLFGLVVVAAVGSSVPVLIMTLSVIYI
PGAYRFARALAVNINAMDFITVARIRGESTLYLIRSEILPNIVGPVLADLGIRFVFIVLLLSGLSFLGLGVQPPYADWGA
LVRENIGGLPFGAPAVMFPSLAIASLTISVNLLIDNLPQKIRDRSVS

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787054, Length=264, Percent_Identity=34.0909090909091, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787760, Length=293, Percent_Identity=30.7167235494881, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1787549, Length=256, Percent_Identity=31.640625, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789964, Length=248, Percent_Identity=33.0645161290323, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789889, Length=245, Percent_Identity=25.7142857142857, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787498, Length=276, Percent_Identity=26.8115942028986, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30895; Mature: 30764

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMTSSETTSGRLSGTRFGYRFNIVGAIGFTVILLWALVAIFAPWVIPYPVGEIIDLDYF
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEECCCC
GPMSRELWLGSDYLGRDMLSRILMGARYTVGISLAAVTIACFCGVVLGMIAAVAGGWLDT
CCCCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
ILSRFLDALNSIPSKLFGLVVVAAVGSSVPVLIMTLSVIYIPGAYRFARALAVNINAMDF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
ITVARIRGESTLYLIRSEILPNIVGPVLADLGIRFVFIVLLLSGLSFLGLGVQPPYADWG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ALVRENIGGLPFGAPAVMFPSLAIASLTISVNLLIDNLPQKIRDRSVS
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TMTSSETTSGRLSGTRFGYRFNIVGAIGFTVILLWALVAIFAPWVIPYPVGEIIDLDYF
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEECCCC
GPMSRELWLGSDYLGRDMLSRILMGARYTVGISLAAVTIACFCGVVLGMIAAVAGGWLDT
CCCCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
ILSRFLDALNSIPSKLFGLVVVAAVGSSVPVLIMTLSVIYIPGAYRFARALAVNINAMDF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
ITVARIRGESTLYLIRSEILPNIVGPVLADLGIRFVFIVLLLSGLSFLGLGVQPPYADWG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ALVRENIGGLPFGAPAVMFPSLAIASLTISVNLLIDNLPQKIRDRSVS
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]