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The map label for this gene is folE [H]

Identifier: 116249235

GI number: 116249235

Start: 624341

End: 624949

Strand: Reverse

Name: folE [H]

Synonym: pRL120570

Alternate gene names: 116249235

Gene position: 624949-624341 (Counterclockwise)

Preceding gene: 116249236

Following gene: 116249234

Centisome position: 71.83

GC content: 56.81

Gene sequence:

>609_bases
ATGGATGCCATTGTCACCAATGCATGCCCGGTGGCTGCCTCGGAACGTCCAACTCAACACCAGGCTGAGGAAGCGGTGCG
TAAGCTAATACGCTGGGCCGGAGACGATCCATCACGCGAAGGTCTGCTCGATACTCCGAAGCGGGTGGCAAAGGCTTATC
GCGAACTCTTCTCCGGATACGCAACGGAGCCTCAGGATGTGCTTTCGAAGACTTTTTCCGAAGTCGGTGGCTACAACGAG
ATGGTGCTCGTCAGAGGCATCGAGTTTCATTCCCATTGCGAGCACCACATGGTGCCGTTCCATGGGATTGCTCATATCGC
ATACATTCCCGAGCAGTGCGTGCTCGGTCTTTCAAAAATGGCAAGGCTCGTCGATCTCTATGCCCGCAGGCTGCAGACCC
AGGAAACCATGACTGCTCAGATCGCGAGAGCTCTTGAAGAAAGCTTACGCCCGAAAGGTGTCGCCGTCATGATCGAGGCG
GAGCACATGTGCATGGCAATGCGCGGCATCCGGAAGCAGGGAGCCAAGACGCTCACAACCGCGTTCGCCGGACTGTTCGA
AGCATCTGTATCGGATCAGGCGCGTTTCACAATGCTGGCCCGCGGTTGA

Upstream 100 bases:

>100_bases
GAACCAATCTGACAGTTGCGCTACTTTGCAGATCGGCAGTTTTGATCCGGAACCCGGCAAGGGACGTAAGCTCAGTCGCT
TGTTAATTCCGGAGAGATTT

Downstream 100 bases:

>100_bases
AACCGGCCTCCTGACGACAATGAATCGCAATCTCGAGAACCCCGGATGACCGAAACGCTGATGCGATCATTAGATGCAGG
GTATTCTGCCTTGGTCACTG

Product: putative GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MDAIVTNACPVAASERPTQHQAEEAVRKLIRWAGDDPSREGLLDTPKRVAKAYRELFSGYATEPQDVLSKTFSEVGGYNE
MVLVRGIEFHSHCEHHMVPFHGIAHIAYIPEQCVLGLSKMARLVDLYARRLQTQETMTAQIARALEESLRPKGVAVMIEA
EHMCMAMRGIRKQGAKTLTTAFAGLFEASVSDQARFTMLARG

Sequences:

>Translated_202_residues
MDAIVTNACPVAASERPTQHQAEEAVRKLIRWAGDDPSREGLLDTPKRVAKAYRELFSGYATEPQDVLSKTFSEVGGYNE
MVLVRGIEFHSHCEHHMVPFHGIAHIAYIPEQCVLGLSKMARLVDLYARRLQTQETMTAQIARALEESLRPKGVAVMIEA
EHMCMAMRGIRKQGAKTLTTAFAGLFEASVSDQARFTMLARG
>Mature_202_residues
MDAIVTNACPVAASERPTQHQAEEAVRKLIRWAGDDPSREGLLDTPKRVAKAYRELFSGYATEPQDVLSKTFSEVGGYNE
MVLVRGIEFHSHCEHHMVPFHGIAHIAYIPEQCVLGLSKMARLVDLYARRLQTQETMTAQIARALEESLRPKGVAVMIEA
EHMCMAMRGIRKQGAKTLTTAFAGLFEASVSDQARFTMLARG

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=196, Percent_Identity=44.3877551020408, Blast_Score=167, Evalue=5e-42,
Organism=Homo sapiens, GI4503949, Length=196, Percent_Identity=44.3877551020408, Blast_Score=167, Evalue=5e-42,
Organism=Homo sapiens, GI66932970, Length=165, Percent_Identity=43.6363636363636, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI66932972, Length=128, Percent_Identity=51.5625, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI1788476, Length=193, Percent_Identity=39.8963730569948, Blast_Score=122, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560486, Length=176, Percent_Identity=46.5909090909091, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6321706, Length=177, Percent_Identity=49.7175141242938, Blast_Score=176, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24656782, Length=178, Percent_Identity=43.8202247191011, Blast_Score=147, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24656796, Length=178, Percent_Identity=43.8202247191011, Blast_Score=147, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24656791, Length=176, Percent_Identity=44.3181818181818, Blast_Score=147, Evalue=6e-36,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 22446; Mature: 22446

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAIVTNACPVAASERPTQHQAEEAVRKLIRWAGDDPSREGLLDTPKRVAKAYRELFSGY
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ATEPQDVLSKTFSEVGGYNEMVLVRGIEFHSHCEHHMVPFHGIAHIAYIPEQCVLGLSKM
CCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ARLVDLYARRLQTQETMTAQIARALEESLRPKGVAVMIEAEHMCMAMRGIRKQGAKTLTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
AFAGLFEASVSDQARFTMLARG
HHHHHHHHCCCCCHHHHHEECC
>Mature Secondary Structure
MDAIVTNACPVAASERPTQHQAEEAVRKLIRWAGDDPSREGLLDTPKRVAKAYRELFSGY
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ATEPQDVLSKTFSEVGGYNEMVLVRGIEFHSHCEHHMVPFHGIAHIAYIPEQCVLGLSKM
CCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ARLVDLYARRLQTQETMTAQIARALEESLRPKGVAVMIEAEHMCMAMRGIRKQGAKTLTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
AFAGLFEASVSDQARFTMLARG
HHHHHHHHCCCCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA