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The map label for this gene is butA [H]

Identifier: 116249213

GI number: 116249213

Start: 596714

End: 597241

Strand: Reverse

Name: butA [H]

Synonym: pRL120549

Alternate gene names: 116249213

Gene position: 597241-596714 (Counterclockwise)

Preceding gene: 116249214

Following gene: 116249211

Centisome position: 68.65

GC content: 59.47

Gene sequence:

>528_bases
GTGGACAAGGTTGTCGCAAAATACGGCCGCATCGACATTCTCGTGAACAATGCTTTCGATGGTTCTGCGGTCTTGTCTTC
AGTCATCGACCTTTCGGTCGAGCAGCTGCAACGGAACTTCGACACCGGGCCGATCGCATATCTACGCTTCATGCAGGCTT
GCTATCCTCATCTGAGAGAAGGCGGAGAAGGGCGGATCATCAACTTCGGGTCGATGGCCGGCACGATTGGATTGGCCGGG
TACGGCCCGTACAACATGGCCAAGGAGGCGGTGCGCGCGCTCACTCGCACGGCGGCACGCGAATGGGGTGCCGACAAGAT
CACCGTGAACAACGTTCTGCCGGTAGCGGACACTTGGGGCGCAGCAGAAAAGGAGGTTCCTCCACCGATGAGCGCACTTG
CTCGTTTCGGCTCGCCGGAAGACGACATCGCCCCGGTGGTGCTGTTCCTGGCCAGCAAGGATTCCCAATTCATTACGGGA
TACAGCCTTACCCCCGACGGTGGCGCCATCATCGACAGCGCCCGGTAA

Upstream 100 bases:

>100_bases
CGAAGGCGTCGAACGCGTTGTCGCGGATATCGCCGAAGCCGGCGGCACCGCGCTCGGCGTCGTCTGCGACGTCGGTGAAG
CCGATCAGATCACGGCCGCG

Downstream 100 bases:

>100_bases
TTGTCGATGAACAAGAGGCGCGACGGGTTGCGCTGCTTCGCACGGCCTGTTTTCCGCTACCGATACCGGTAGCGGAAAGC
TTCAACGAAGGCGGAGAAAG

Product: putative short-chain dehydrogenase/reductase family protein

Products: NA

Alternate protein names: Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MDKVVAKYGRIDILVNNAFDGSAVLSSVIDLSVEQLQRNFDTGPIAYLRFMQACYPHLREGGEGRIINFGSMAGTIGLAG
YGPYNMAKEAVRALTRTAAREWGADKITVNNVLPVADTWGAAEKEVPPPMSALARFGSPEDDIAPVVLFLASKDSQFITG
YSLTPDGGAIIDSAR

Sequences:

>Translated_175_residues
MDKVVAKYGRIDILVNNAFDGSAVLSSVIDLSVEQLQRNFDTGPIAYLRFMQACYPHLREGGEGRIINFGSMAGTIGLAG
YGPYNMAKEAVRALTRTAAREWGADKITVNNVLPVADTWGAAEKEVPPPMSALARFGSPEDDIAPVVLFLASKDSQFITG
YSLTPDGGAIIDSAR
>Mature_175_residues
MDKVVAKYGRIDILVNNAFDGSAVLSSVIDLSVEQLQRNFDTGPIAYLRFMQACYPHLREGGEGRIINFGSMAGTIGLAG
YGPYNMAKEAVRALTRTAAREWGADKITVNNVLPVADTWGAAEKEVPPPMSALARFGSPEDDIAPVVLFLASKDSQFITG
YSLTPDGGAIIDSAR

Specific function: Catalyzes the irreversible reduction of 2,3-butanediol to (S)-acetoin in the presence of NADH [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=172, Percent_Identity=32.5581395348837, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI15277342, Length=128, Percent_Identity=39.0625, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1788459, Length=178, Percent_Identity=33.1460674157303, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1789208, Length=182, Percent_Identity=31.8681318681319, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1787335, Length=178, Percent_Identity=30.8988764044944, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI2367365, Length=170, Percent_Identity=31.7647058823529, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1790717, Length=179, Percent_Identity=29.608938547486, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI87082100, Length=188, Percent_Identity=28.7234042553192, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787905, Length=176, Percent_Identity=28.4090909090909, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=182, Percent_Identity=31.8681318681319, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560150, Length=192, Percent_Identity=31.25, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17561402, Length=179, Percent_Identity=31.2849162011173, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=187, Percent_Identity=32.0855614973262, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994604, Length=182, Percent_Identity=31.3186813186813, Blast_Score=66, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322861, Length=156, Percent_Identity=30.1282051282051, Blast_Score=65, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21357041, Length=176, Percent_Identity=33.5227272727273, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28571526, Length=175, Percent_Identity=33.1428571428571, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643142, Length=172, Percent_Identity=30.8139534883721, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI23397609, Length=180, Percent_Identity=30, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24644339, Length=182, Percent_Identity=30.2197802197802, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355319, Length=175, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014007
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.304 [H]

Molecular weight: Translated: 18741; Mature: 18741

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKVVAKYGRIDILVNNAFDGSAVLSSVIDLSVEQLQRNFDTGPIAYLRFMQACYPHLRE
CCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
GGEGRIINFGSMAGTIGLAGYGPYNMAKEAVRALTRTAAREWGADKITVNNVLPVADTWG
CCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCCCCC
AAEKEVPPPMSALARFGSPEDDIAPVVLFLASKDSQFITGYSLTPDGGAIIDSAR
CCCCCCCCCHHHHHHCCCCCCCHHHEEHEEECCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MDKVVAKYGRIDILVNNAFDGSAVLSSVIDLSVEQLQRNFDTGPIAYLRFMQACYPHLRE
CCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
GGEGRIINFGSMAGTIGLAGYGPYNMAKEAVRALTRTAAREWGADKITVNNVLPVADTWG
CCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCCCCC
AAEKEVPPPMSALARFGSPEDDIAPVVLFLASKDSQFITGYSLTPDGGAIIDSAR
CCCCCCCCCHHHHHHCCCCCCCHHHEEHEEECCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA