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The map label for this gene is etfA [H]

Identifier: 116249186

GI number: 116249186

Start: 567332

End: 568273

Strand: Direct

Name: etfA [H]

Synonym: pRL120522

Alternate gene names: 116249186

Gene position: 567332-568273 (Clockwise)

Preceding gene: 116249185

Following gene: 116249187

Centisome position: 65.21

GC content: 63.38

Gene sequence:

>942_bases
ATGACCATTCTTCTTCTGGCTGACCACGACAATGCAAGCCTGTCCGACCAGACCGCCAAGACGCTGACGGCTGCCGCAAA
GATTGCCAAGGACCAAGCAAGCGATATCCACATTCTCGTCGCCGGCAAAGCTGCCAAGGCTGCTGCCGATGCCGCCGCCA
AGCTTGCCGGCGTCTCCAAGGTGCTGCTGGCCGAAAGCGACGAACTCGCCAACAATCTGGCCGAACCGCTGGCCGACCTG
ATCGTCTCGCTGGCCGGCAGCTACGACACGATCATCTCGGCTGCCACCTCGGTCGGCAAGAATGTGCTGCCGCGGGTCGC
TGCCCTTCTCGATGTCGCCCAGGTCTCGGAGATCATCGAGGTGATCTCGTCCGACACCTTCAAGCGGCCGATCTATGCCG
GCAATGCCATCCAGACGGTGCAGGCAAGCGACGCGAAGAAGGTGATCACCGTGCGCACCGCCTCCTTCGCCTCTGCACCG
GAAGGCGGATCGGCCACCGTCGAAGCGATCCCGGCGGTTTCCGATCCGGGACTGTCGACCTTCGTCAGGGACGCGCTGTC
GGCCTCCGACCGTCCGGAATTGACCTCTGCGAAGATCATCATCTCCGGCGGCCGGGCGCTCGGCTCGGCGGAGAAGTTCA
GGGAAGTCATCCTGCCGCTTGCCGACAAGCTCGGTGCTGCCGTCGGTGCATCCAGAGCGGCCGTCGATGCCGGTTATGCG
CCGAACGACTGGCAGGTCGGCCAGACCGGCAAGGTGGTGGCGCCGCAGCTCTATATTGCCTGCGGCATTTCAGGCGCCAT
CCAGCATCTGGCCGGCATGAAGGATTCGAAGGTCATCGTCGCCATCAACAAGGACGAGGAGGCGCCGATCTTCCAGGTCG
CCGACTACGGATTGGTCGCCGATCTCTTCGACGCCCTGCCGGAATTGCAAAAGGCGCTCTAG

Upstream 100 bases:

>100_bases
CCGCAAGGCCGGCGTCAAGGTCAAGTCGGTCGCCGAACTCATCGACAAGCTCAAAAACGAAGCCGGCGTGCTGTAATCAG
TTGGAACAGGAGCCATCATC

Downstream 100 bases:

>100_bases
CGGGGGAGACCGAACGTGGCGCGCGTCGTGGGACGTCCAATGAAACCTGGTGCCGACCTTCTCCTGCACGATGACGAGAA
GCCGGCATACATACGGCTGC

Product: putative electron transfer flavoprotein alpha-subunit

Products: NA

Alternate protein names: Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTILLLADHDNASLSDQTAKTLTAAAKIAKDQASDIHILVAGKAAKAAADAAAKLAGVSKVLLAESDELANNLAEPLADL
IVSLAGSYDTIISAATSVGKNVLPRVAALLDVAQVSEIIEVISSDTFKRPIYAGNAIQTVQASDAKKVITVRTASFASAP
EGGSATVEAIPAVSDPGLSTFVRDALSASDRPELTSAKIIISGGRALGSAEKFREVILPLADKLGAAVGASRAAVDAGYA
PNDWQVGQTGKVVAPQLYIACGISGAIQHLAGMKDSKVIVAINKDEEAPIFQVADYGLVADLFDALPELQKAL

Sequences:

>Translated_313_residues
MTILLLADHDNASLSDQTAKTLTAAAKIAKDQASDIHILVAGKAAKAAADAAAKLAGVSKVLLAESDELANNLAEPLADL
IVSLAGSYDTIISAATSVGKNVLPRVAALLDVAQVSEIIEVISSDTFKRPIYAGNAIQTVQASDAKKVITVRTASFASAP
EGGSATVEAIPAVSDPGLSTFVRDALSASDRPELTSAKIIISGGRALGSAEKFREVILPLADKLGAAVGASRAAVDAGYA
PNDWQVGQTGKVVAPQLYIACGISGAIQHLAGMKDSKVIVAINKDEEAPIFQVADYGLVADLFDALPELQKAL
>Mature_312_residues
TILLLADHDNASLSDQTAKTLTAAAKIAKDQASDIHILVAGKAAKAAADAAAKLAGVSKVLLAESDELANNLAEPLADLI
VSLAGSYDTIISAATSVGKNVLPRVAALLDVAQVSEIIEVISSDTFKRPIYAGNAIQTVQASDAKKVITVRTASFASAPE
GGSATVEAIPAVSDPGLSTFVRDALSASDRPELTSAKIIISGGRALGSAEKFREVILPLADKLGAAVGASRAAVDAGYAP
NDWQVGQTGKVVAPQLYIACGISGAIQHLAGMKDSKVIVAINKDEEAPIFQVADYGLVADLFDALPELQKAL

Specific function: The electron transfer flavoprotein serves as a specific electron acceptor for other dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) [H]

COG id: COG2025

COG function: function code C; Electron transfer flavoprotein, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF alpha-subunit/fixB family [H]

Homologues:

Organism=Homo sapiens, GI4503607, Length=315, Percent_Identity=53.6507936507937, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI189181759, Length=275, Percent_Identity=56.7272727272727, Blast_Score=288, Evalue=5e-78,
Organism=Escherichia coli, GI1786226, Length=199, Percent_Identity=37.1859296482412, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787990, Length=257, Percent_Identity=30.7392996108949, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082157, Length=224, Percent_Identity=29.9107142857143, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17506929, Length=315, Percent_Identity=53.3333333333333, Blast_Score=309, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6325261, Length=289, Percent_Identity=47.4048442906574, Blast_Score=251, Evalue=7e-68,
Organism=Drosophila melanogaster, GI17136898, Length=317, Percent_Identity=56.4668769716088, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24652801, Length=317, Percent_Identity=56.4668769716088, Blast_Score=318, Evalue=4e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001308
- InterPro:   IPR014730
- InterPro:   IPR014731
- InterPro:   IPR018206
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01012 ETF; PF00766 ETF_alpha [H]

EC number: NA

Molecular weight: Translated: 32022; Mature: 31891

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00696 ETF_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILLLADHDNASLSDQTAKTLTAAAKIAKDQASDIHILVAGKAAKAAADAAAKLAGVSK
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
VLLAESDELANNLAEPLADLIVSLAGSYDTIISAATSVGKNVLPRVAALLDVAQVSEIIE
HHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VISSDTFKRPIYAGNAIQTVQASDAKKVITVRTASFASAPEGGSATVEAIPAVSDPGLST
HHHCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHH
FVRDALSASDRPELTSAKIIISGGRALGSAEKFREVILPLADKLGAAVGASRAAVDAGYA
HHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
PNDWQVGQTGKVVAPQLYIACGISGAIQHLAGMKDSKVIVAINKDEEAPIFQVADYGLVA
CCCCCCCCCCCEECCEEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCHHHH
DLFDALPELQKAL
HHHHHHHHHHHCC
>Mature Secondary Structure 
TILLLADHDNASLSDQTAKTLTAAAKIAKDQASDIHILVAGKAAKAAADAAAKLAGVSK
EEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
VLLAESDELANNLAEPLADLIVSLAGSYDTIISAATSVGKNVLPRVAALLDVAQVSEIIE
HHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VISSDTFKRPIYAGNAIQTVQASDAKKVITVRTASFASAPEGGSATVEAIPAVSDPGLST
HHHCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHH
FVRDALSASDRPELTSAKIIISGGRALGSAEKFREVILPLADKLGAAVGASRAAVDAGYA
HHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
PNDWQVGQTGKVVAPQLYIACGISGAIQHLAGMKDSKVIVAINKDEEAPIFQVADYGLVA
CCCCCCCCCCCEECCEEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCHHHH
DLFDALPELQKAL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8599534; 12597275 [H]