BacMap

Definition	Rhizobium leguminosarum bv. viciae 3841 plasmid pRL12, complete sequence.
Accession	NC_008378
Length	870,021

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The map label for this gene is impN

Identifier: 116249127

GI number: 116249127

Start: 496311

End: 497225

Strand: Reverse

Name: impN

Synonym: pRL120462

Alternate gene names: NA

Gene position: 497225-496311 (Counterclockwise)

Preceding gene: 116249128

Following gene: 116249125

Centisome position: 57.15

GC content: 61.31

Gene sequence:

>915_bases
ATGACAAATGATCATCCTGCGGATTTCGAAGAGATCGCCCGACGTTTTTCGCATCTCTGGCAGGCGTTGAATCGCGGTTC
ACCCGAGACCCGGAAAATCCTGGTAGACCGCGTCCGGGACGCGCTCGATCGGGGCGAGGCCCGCCTCCGCGACGCGGATT
GCGAGATCGTCGCCAACAGCTTTATGCTCGAGGCGCTGATCCATGACGGCGCGGCCACCCGGGTCTATCGCGCCCGCCAT
CGCGATCTCGGAACGTTTCATGCCATCAAGATGCCGCAGGCCGACCACGCCGACGATCCCGTCCTCAGAAAACTGATGCT
GCGCGAGGCGGAAATCGGCATGGCGCTCCGCCATCCCCACATTGCCGAGACGCAGACCGTTCTGCGCCTTGCCGACGGGC
GCCCTGCCCTGGTCATGGAATGGACCGGCAGCCGCCTTGCGGAGCTTTTGCAGACAAGGTCATTATCCTTCGGCGAGATC
ACTTCAGCGATGACCTCAGTGCTTTCAGGCCTGGAGGCCATCCATGCCGGCGGTTTCGTGCATTGCGACCTGTCGCCTCT
CAACCTTCTCGCCGACGGCGATCTCTCCCGTTTGAAGATCGCCGATTTCGGCATCGCGCTTCAGCGCGGATGCCGGCATG
GTCAATTCGATCTTGCCTTTGCCGGCCAGCCGGATTTCGCATCTCCTGAACAGATGGCCGGTGAACCGCTCGACGAAAGA
TCCGATCTTTACGCAGCCGGGTGTCTGCTGTCCTTGCTGCTTAGCCGTTGCAACGAGGAGAGCGACGAAACCGAAGCCCT
CAAGGCATTGGCGAACCGCCTGTCCCAACAGGATCCGCAGGACAGGCCCGAAAATGCAAGAGCCGCCTTGCGTCTGCTGG
GCGGCTCCGCAAAACGTCCTGTTGTCGATGCTTAA

Upstream 100 bases:

>100_bases
TCCGCAAAGATCGAAAAGGCCGCCGATATCCTCGTCCAGGAGGGGCTGATTGCCAATTGCCGCGAAAACCTCAGCGCAGT
CGTGATCGATGTGAAGACCG

Downstream 100 bases:

>100_bases
TTGCCGCTCGACCCCGGGATCGGTGCTGCGGCCGTCGGCACGCTTTTCTGGTACACGTTGAAGAGCTTGGCAAAGTCCAC
CAGGCTGGCGTTGCTGGCGT

Product: putative serine/threonine-protein kinase involved in nitrogen fixation

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTNDHPADFEEIARRFSHLWQALNRGSPETRKILVDRVRDALDRGEARLRDADCEIVANSFMLEALIHDGAATRVYRARH
RDLGTFHAIKMPQADHADDPVLRKLMLREAEIGMALRHPHIAETQTVLRLADGRPALVMEWTGSRLAELLQTRSLSFGEI
TSAMTSVLSGLEAIHAGGFVHCDLSPLNLLADGDLSRLKIADFGIALQRGCRHGQFDLAFAGQPDFASPEQMAGEPLDER
SDLYAAGCLLSLLLSRCNEESDETEALKALANRLSQQDPQDRPENARAALRLLGGSAKRPVVDA

Sequences:

>Translated_304_residues
MTNDHPADFEEIARRFSHLWQALNRGSPETRKILVDRVRDALDRGEARLRDADCEIVANSFMLEALIHDGAATRVYRARH
RDLGTFHAIKMPQADHADDPVLRKLMLREAEIGMALRHPHIAETQTVLRLADGRPALVMEWTGSRLAELLQTRSLSFGEI
TSAMTSVLSGLEAIHAGGFVHCDLSPLNLLADGDLSRLKIADFGIALQRGCRHGQFDLAFAGQPDFASPEQMAGEPLDER
SDLYAAGCLLSLLLSRCNEESDETEALKALANRLSQQDPQDRPENARAALRLLGGSAKRPVVDA
>Mature_303_residues
TNDHPADFEEIARRFSHLWQALNRGSPETRKILVDRVRDALDRGEARLRDADCEIVANSFMLEALIHDGAATRVYRARHR
DLGTFHAIKMPQADHADDPVLRKLMLREAEIGMALRHPHIAETQTVLRLADGRPALVMEWTGSRLAELLQTRSLSFGEIT
SAMTSVLSGLEAIHAGGFVHCDLSPLNLLADGDLSRLKIADFGIALQRGCRHGQFDLAFAGQPDFASPEQMAGEPLDERS
DLYAAGCLLSLLLSRCNEESDETEALKALANRLSQQDPQDRPENARAALRLLGGSAKRPVVDA

Specific function: Unknown

COG id: COG0515

COG function: function code RTKL; Serine/threonine protein kinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Homo sapiens, GI14042966, Length=254, Percent_Identity=26.3779527559055, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI27901803, Length=203, Percent_Identity=30.0492610837438, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI7662170, Length=197, Percent_Identity=32.48730964467, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI153791472, Length=199, Percent_Identity=30.1507537688442, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI193083125, Length=226, Percent_Identity=30.0884955752212, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI193083127, Length=226, Percent_Identity=30.0884955752212, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI46852166, Length=226, Percent_Identity=30.0884955752212, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI193083129, Length=226, Percent_Identity=30.0884955752212, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI254028232, Length=220, Percent_Identity=28.6363636363636, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI254028240, Length=220, Percent_Identity=28.6363636363636, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI254028237, Length=220, Percent_Identity=28.6363636363636, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI254028234, Length=220, Percent_Identity=28.6363636363636, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI86990441, Length=215, Percent_Identity=29.3023255813954, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI110349799, Length=221, Percent_Identity=27.6018099547511, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4557511, Length=190, Percent_Identity=28.9473684210526, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI26667189, Length=206, Percent_Identity=29.6116504854369, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI26667183, Length=206, Percent_Identity=29.6116504854369, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI26667186, Length=206, Percent_Identity=29.6116504854369, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI212549753, Length=206, Percent_Identity=29.6116504854369, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI45439359, Length=172, Percent_Identity=32.5581395348837, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI212549756, Length=206, Percent_Identity=29.6116504854369, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI26667180, Length=206, Percent_Identity=29.6116504854369, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI41872374, Length=218, Percent_Identity=26.1467889908257, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI13569922, Length=229, Percent_Identity=29.2576419213974, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI66932999, Length=205, Percent_Identity=30.2439024390244, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI25952118, Length=152, Percent_Identity=33.5526315789474, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI16418343, Length=202, Percent_Identity=26.2376237623762, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI25952114, Length=152, Percent_Identity=33.5526315789474, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI7657214, Length=238, Percent_Identity=27.3109243697479, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI30039692, Length=206, Percent_Identity=29.6116504854369, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI26667211, Length=152, Percent_Identity=32.8947368421053, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI26667199, Length=152, Percent_Identity=32.8947368421053, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI26667196, Length=152, Percent_Identity=32.8947368421053, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI26667191, Length=152, Percent_Identity=32.8947368421053, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI116256471, Length=197, Percent_Identity=29.9492385786802, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI26667203, Length=208, Percent_Identity=29.3269230769231, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI42794767, Length=243, Percent_Identity=27.5720164609054, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI42794765, Length=243, Percent_Identity=27.5720164609054, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI217330634, Length=188, Percent_Identity=29.2553191489362, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI148833506, Length=190, Percent_Identity=28.9473684210526, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71991623, Length=191, Percent_Identity=29.8429319371728, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71991647, Length=191, Percent_Identity=29.8429319371728, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71991657, Length=191, Percent_Identity=29.8429319371728, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71991638, Length=191, Percent_Identity=29.8429319371728, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71991652, Length=191, Percent_Identity=29.8429319371728, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71991633, Length=191, Percent_Identity=29.8429319371728, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71991629, Length=191, Percent_Identity=29.8429319371728, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71991643, Length=203, Percent_Identity=28.0788177339901, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71991618, Length=191, Percent_Identity=29.8429319371728, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17570595, Length=202, Percent_Identity=28.7128712871287, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193208795, Length=238, Percent_Identity=25.2100840336134, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI133901970, Length=197, Percent_Identity=30.4568527918782, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71994328, Length=257, Percent_Identity=28.0155642023346, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17568289, Length=193, Percent_Identity=30.0518134715026, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533246, Length=220, Percent_Identity=28.6363636363636, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71997537, Length=220, Percent_Identity=28.6363636363636, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193208793, Length=214, Percent_Identity=27.1028037383178, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71997531, Length=220, Percent_Identity=28.6363636363636, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6320685, Length=199, Percent_Identity=28.643216080402, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281359587, Length=206, Percent_Identity=28.6407766990291, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62484416, Length=206, Percent_Identity=28.6407766990291, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62484414, Length=206, Percent_Identity=28.6407766990291, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24638772, Length=206, Percent_Identity=28.6407766990291, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281359589, Length=206, Percent_Identity=28.6407766990291, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62473187, Length=206, Percent_Identity=28.6407766990291, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281359585, Length=206, Percent_Identity=28.6407766990291, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45549243, Length=206, Percent_Identity=28.6407766990291, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45551188, Length=206, Percent_Identity=28.6407766990291, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI116007536, Length=225, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI116007530, Length=225, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI116007532, Length=225, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI116007534, Length=225, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI221474064, Length=238, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21358073, Length=221, Percent_Identity=28.0542986425339, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550626, Length=212, Percent_Identity=29.2452830188679, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648121, Length=242, Percent_Identity=26.4462809917355, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17136516, Length=200, Percent_Identity=30, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17137760, Length=193, Percent_Identity=27.4611398963731, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR005543
- InterPro:   IPR000719
- InterPro:   IPR017441
- InterPro:   IPR017442
- InterPro:   IPR008271 [H]

Pfam domain/function: PF03793 PASTA; PF00069 Pkinase [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 33528; Mature: 33397

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50011 PROTEIN_KINASE_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDHPADFEEIARRFSHLWQALNRGSPETRKILVDRVRDALDRGEARLRDADCEIVANS
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FMLEALIHDGAATRVYRARHRDLGTFHAIKMPQADHADDPVLRKLMLREAEIGMALRHPH
HHHHHHHHCCHHHHHHHHHHCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
IAETQTVLRLADGRPALVMEWTGSRLAELLQTRSLSFGEITSAMTSVLSGLEAIHAGGFV
HHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEE
HCDLSPLNLLADGDLSRLKIADFGIALQRGCRHGQFDLAFAGQPDFASPEQMAGEPLDER
EECCCHHHEEECCCCCCEEHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCCCCCCHH
SDLYAAGCLLSLLLSRCNEESDETEALKALANRLSQQDPQDRPENARAALRLLGGSAKRP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC
VVDA
CCCC
>Mature Secondary Structure 
TNDHPADFEEIARRFSHLWQALNRGSPETRKILVDRVRDALDRGEARLRDADCEIVANS
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FMLEALIHDGAATRVYRARHRDLGTFHAIKMPQADHADDPVLRKLMLREAEIGMALRHPH
HHHHHHHHCCHHHHHHHHHHCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
IAETQTVLRLADGRPALVMEWTGSRLAELLQTRSLSFGEITSAMTSVLSGLEAIHAGGFV
HHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEE
HCDLSPLNLLADGDLSRLKIADFGIALQRGCRHGQFDLAFAGQPDFASPEQMAGEPLDER
EECCCHHHEEECCCCCCEEHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCCCCCCHH
SDLYAAGCLLSLLLSRCNEESDETEALKALANRLSQQDPQDRPENARAALRLLGGSAKRP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC
VVDA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]