Click here to switch to the map view.

The map label for this gene is amiR [H]

Identifier: 116249111

GI number: 116249111

Start: 480567

End: 481175

Strand: Reverse

Name: amiR [H]

Synonym: pRL120446

Alternate gene names: 116249111

Gene position: 481175-480567 (Counterclockwise)

Preceding gene: 116249112

Following gene: 116249110

Centisome position: 55.31

GC content: 57.8

Gene sequence:

>609_bases
ATGTCTTTCGCCCTTCTCAGAGATTTGCGGGATCTCAAGGTTCTGGTCATCCACCCCCGGACATCCGAAGGAGAGTTTCT
CGTCGATCACCTGCGCCGTATCGGCTGTACGGTCAGTTGCCTTTGGCCGGTGCCTGCCGAGTTGCCGCACGGGACAGATG
TCGTCTTCCTCGCGATGGAAGATGAGGCGCGACCGGAAATCGAACGCCTGCTGAAGTCTTTCCCCAATCCTGCCCCGACC
ATGCTTGCCATCGTCGGTTACGAAAACCCCTCGACGCTGCAGATCGTTCTGGAAAGCGGCGCACTCGCCATTGTCGAAAG
ACCGATCAAACCCTTTGGCCTGCTGACCAATCTCGCCATCGCCCGCAACCTGTGGCTGGAGCGGCAAAAGCTCGTCAAGG
AAGCGCGCAAATACAAACGCAAGGTACTGGGCGACCAGAAACTTGCTCGGGCCAAAGCCATTCTGATGGCCAATCGCGGC
ACCAGCGAGAATGAGGCGTATCGTGAAATGCGCGACCAGGCGATGGCTCGGCGGGTGCCGATCGATGAAATCGCCAATTC
GATTATCAACGCCGAACAGCTCTTGCGCCCGCCCCAAAAACATGATTAG

Upstream 100 bases:

>100_bases
TCCGTCGCTCCGGGTGGCGCCAGACCCGTTCATGCGAGAATATCGCCCGGACTTGACGCGGACTTTCCCGCATCGTGTTC
AGGTTTGAGAGGGAGCGACG

Downstream 100 bases:

>100_bases
TTTTTCACGTGTTTCCAACAAAACACCTCCGGCACATCCCTTCGTTTGACGAAGCCATGTGCCGCTGTTGCACGCGGCGC
AGCCGTGATGCGGTGATGGC

Product: putative ANTAR RNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MSFALLRDLRDLKVLVIHPRTSEGEFLVDHLRRIGCTVSCLWPVPAELPHGTDVVFLAMEDEARPEIERLLKSFPNPAPT
MLAIVGYENPSTLQIVLESGALAIVERPIKPFGLLTNLAIARNLWLERQKLVKEARKYKRKVLGDQKLARAKAILMANRG
TSENEAYREMRDQAMARRVPIDEIANSIINAEQLLRPPQKHD

Sequences:

>Translated_202_residues
MSFALLRDLRDLKVLVIHPRTSEGEFLVDHLRRIGCTVSCLWPVPAELPHGTDVVFLAMEDEARPEIERLLKSFPNPAPT
MLAIVGYENPSTLQIVLESGALAIVERPIKPFGLLTNLAIARNLWLERQKLVKEARKYKRKVLGDQKLARAKAILMANRG
TSENEAYREMRDQAMARRVPIDEIANSIINAEQLLRPPQKHD
>Mature_201_residues
SFALLRDLRDLKVLVIHPRTSEGEFLVDHLRRIGCTVSCLWPVPAELPHGTDVVFLAMEDEARPEIERLLKSFPNPAPTM
LAIVGYENPSTLQIVLESGALAIVERPIKPFGLLTNLAIARNLWLERQKLVKEARKYKRKVLGDQKLARAKAILMANRGT
SENEAYREMRDQAMARRVPIDEIANSIINAEQLLRPPQKHD

Specific function: Positive controlling element of AmiE, the gene for aliphatic amidase. Acts as a transcriptional antitermination factor. It is thought to allow RNA polymerase read through a rho- independent transcription terminator between the AmiE promoter and gene [H]

COG id: COG3707

COG function: function code T; Response regulator with putative antiterminator output domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ANTAR domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005561
- InterPro:   IPR011006
- InterPro:   IPR008327
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03861 ANTAR [H]

EC number: NA

Molecular weight: Translated: 22905; Mature: 22774

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50921 ANTAR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFALLRDLRDLKVLVIHPRTSEGEFLVDHLRRIGCTVSCLWPVPAELPHGTDVVFLAME
CCHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEEEC
DEARPEIERLLKSFPNPAPTMLAIVGYENPSTLQIVLESGALAIVERPIKPFGLLTNLAI
CCCCHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCEEEHHCCCCHHHHHHHHHH
ARNLWLERQKLVKEARKYKRKVLGDQKLARAKAILMANRGTSENEAYREMRDQAMARRVP
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHCCC
IDEIANSIINAEQLLRPPQKHD
HHHHHHHHHCHHHHCCCCCCCC
>Mature Secondary Structure 
SFALLRDLRDLKVLVIHPRTSEGEFLVDHLRRIGCTVSCLWPVPAELPHGTDVVFLAME
CHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEEEC
DEARPEIERLLKSFPNPAPTMLAIVGYENPSTLQIVLESGALAIVERPIKPFGLLTNLAI
CCCCHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCEEEHHCCCCHHHHHHHHHH
ARNLWLERQKLVKEARKYKRKVLGDQKLARAKAILMANRGTSENEAYREMRDQAMARRVP
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHCCC
IDEIANSIINAEQLLRPPQKHD
HHHHHHHHHCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2495988; 10984043; 7539417; 10508151 [H]