Click here to switch to the map view.

The map label for this gene is thcR [H]

Identifier: 116249084

GI number: 116249084

Start: 452715

End: 453737

Strand: Direct

Name: thcR [H]

Synonym: pRL120418

Alternate gene names: 116249084

Gene position: 452715-453737 (Clockwise)

Preceding gene: 116249083

Following gene: 116249086

Centisome position: 52.03

GC content: 57.87

Gene sequence:

>1023_bases
ATGTCCGTTAATTCTCATATACAAAACGATGGCAGCCTCGCCCGACAGCTGGCGGTGCAGAGCATGTTGTTTCGGGAAAC
GGCGCACAGCGGAACGGACCCGGATGAACTCTCGGAAATATTGTCGACGCCAACCTCCCCGATCAAGGTTGCGGCGGAAG
GCAATATGCCGATTGCATACCACTGCAATTTCGTCTCCGTGGGAGAAGAGGTAACGGTAGCCGATTGTACCTACGAAGGC
ACAATCCTGATCAGGCGGGAGGCGCCCAGCGATAGGATGATCGTTTTTCTGCCGATGGCAGGAAACGCATCCTTCGAAGG
CATGCGGGAGCAAATATATTCCATTCCCGCGCGTGGCACGATCCTTGAGGCAGGTCGTGCCGCGGGTGCTCGCCTGTTTG
GGCCGCGCCGTCATTTCGGCCTGTTCGTCGATCAGGCGAAGATCATTAGCCACCTAACGCATATGTTCGAGAGAACGATC
AGCGGCGACGTCGATTTTCATCCTCACATCGATCTGACGACCGGTCCCGGGCTTGTATTCCAGCAACTTGTCTCGAGCCT
CCATCGCGGCCTCAGCGGCAACGGACCGCTGCAACGCTCGCCGTTGGCGGCCAGCTTGCTCTGTGACGCGGCGATCTATC
TGCTTCTGGAGACCTGCCCCAATCGTTATTCGAACGAGCTTGCGCTCCCTGCTCCAGCGCCGGCTCCACGCCATGTGAAA
TGGGCCATCGACTTCATGCAGGAACACATCGCCGAGCCGATTTCGCTCAACGACATTGCGATTGCAGCCAAAGTCAGCGT
TCGGACTTTGCAACAGGGTTTCCGCCAGTTCAGGAATACGACCCCGATGTCCTACCTGCATGACCTTCGGATGGCCGCCG
CCCATCGCGATTTGCTCGAGTCTGACGAGAAGCAAGCCATCGCCGACGTCGCGCTGCGATGGGGCTTTACGCATCTTGGG
CGATTTTCTGCCGAATACAGGAAGCGTTTCGGCCAGTTGCCGTCACAGACCTTGAGGCGCTGA

Upstream 100 bases:

>100_bases
ACCCGCTACGGCTGAAACCCGGGGCAACACAGAAAAAGAGGGATGAACATGATTGCGCAAATCCATTATCACTTGTGTAC
ATCGCCTTTCGAGGTCACGA

Downstream 100 bases:

>100_bases
GCTCTATCTCGGCGTGGATGATTCCGTCGACGATGGACCAGGCGTCCGATCGCCGACGGGAGCGGCACGCGGGATACGGA
TCTATGCCGCGCGCTTCAGG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MSVNSHIQNDGSLARQLAVQSMLFRETAHSGTDPDELSEILSTPTSPIKVAAEGNMPIAYHCNFVSVGEEVTVADCTYEG
TILIRREAPSDRMIVFLPMAGNASFEGMREQIYSIPARGTILEAGRAAGARLFGPRRHFGLFVDQAKIISHLTHMFERTI
SGDVDFHPHIDLTTGPGLVFQQLVSSLHRGLSGNGPLQRSPLAASLLCDAAIYLLLETCPNRYSNELALPAPAPAPRHVK
WAIDFMQEHIAEPISLNDIAIAAKVSVRTLQQGFRQFRNTTPMSYLHDLRMAAAHRDLLESDEKQAIADVALRWGFTHLG
RFSAEYRKRFGQLPSQTLRR

Sequences:

>Translated_340_residues
MSVNSHIQNDGSLARQLAVQSMLFRETAHSGTDPDELSEILSTPTSPIKVAAEGNMPIAYHCNFVSVGEEVTVADCTYEG
TILIRREAPSDRMIVFLPMAGNASFEGMREQIYSIPARGTILEAGRAAGARLFGPRRHFGLFVDQAKIISHLTHMFERTI
SGDVDFHPHIDLTTGPGLVFQQLVSSLHRGLSGNGPLQRSPLAASLLCDAAIYLLLETCPNRYSNELALPAPAPAPRHVK
WAIDFMQEHIAEPISLNDIAIAAKVSVRTLQQGFRQFRNTTPMSYLHDLRMAAAHRDLLESDEKQAIADVALRWGFTHLG
RFSAEYRKRFGQLPSQTLRR
>Mature_339_residues
SVNSHIQNDGSLARQLAVQSMLFRETAHSGTDPDELSEILSTPTSPIKVAAEGNMPIAYHCNFVSVGEEVTVADCTYEGT
ILIRREAPSDRMIVFLPMAGNASFEGMREQIYSIPARGTILEAGRAAGARLFGPRRHFGLFVDQAKIISHLTHMFERTIS
GDVDFHPHIDLTTGPGLVFQQLVSSLHRGLSGNGPLQRSPLAASLLCDAAIYLLLETCPNRYSNELALPAPAPAPRHVKW
AIDFMQEHIAEPISLNDIAIAAKVSVRTLQQGFRQFRNTTPMSYLHDLRMAAAHRDLLESDEKQAIADVALRWGFTHLGR
FSAEYRKRFGQLPSQTLRR

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=100, Percent_Identity=33, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 37639; Mature: 37508

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNSHIQNDGSLARQLAVQSMLFRETAHSGTDPDELSEILSTPTSPIKVAAEGNMPIAY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEE
HCNFVSVGEEVTVADCTYEGTILIRREAPSDRMIVFLPMAGNASFEGMREQIYSIPARGT
ECCEEECCCEEEEEECEECCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCC
ILEAGRAAGARLFGPRRHFGLFVDQAKIISHLTHMFERTISGDVDFHPHIDLTTGPGLVF
EECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHH
QQLVSSLHRGLSGNGPLQRSPLAASLLCDAAIYLLLETCPNRYSNELALPAPAPAPRHVK
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCEECCCCCCCCCHHH
WAIDFMQEHIAEPISLNDIAIAAKVSVRTLQQGFRQFRNTTPMSYLHDLRMAAAHRDLLE
HHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SDEKQAIADVALRWGFTHLGRFSAEYRKRFGQLPSQTLRR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SVNSHIQNDGSLARQLAVQSMLFRETAHSGTDPDELSEILSTPTSPIKVAAEGNMPIAY
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEE
HCNFVSVGEEVTVADCTYEGTILIRREAPSDRMIVFLPMAGNASFEGMREQIYSIPARGT
ECCEEECCCEEEEEECEECCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCC
ILEAGRAAGARLFGPRRHFGLFVDQAKIISHLTHMFERTISGDVDFHPHIDLTTGPGLVF
EECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHH
QQLVSSLHRGLSGNGPLQRSPLAASLLCDAAIYLLLETCPNRYSNELALPAPAPAPRHVK
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCEECCCCCCCCCHHH
WAIDFMQEHIAEPISLNDIAIAAKVSVRTLQQGFRQFRNTTPMSYLHDLRMAAAHRDLLE
HHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SDEKQAIADVALRWGFTHLGRFSAEYRKRFGQLPSQTLRR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]