Click here to switch to the map view.

The map label for this gene is pcm [H]

Identifier: 116249044

GI number: 116249044

Start: 408099

End: 409010

Strand: Reverse

Name: pcm [H]

Synonym: pRL120378

Alternate gene names: 116249044

Gene position: 409010-408099 (Counterclockwise)

Preceding gene: 116249045

Following gene: 116249043

Centisome position: 47.01

GC content: 63.16

Gene sequence:

>912_bases
ATGCCCGGAGATTTGCCCATGCCGACTACGATGTTCACGGCCGACGAACTGGCGGTGATCCGCCGCGCTTACGCGCATCA
GGTGATGGCAACCGCCGCAACGACGAACCCGCGGATCGAAGCTGCCTTTGCCACCGTCCCGCGCGAAAAATTCCTCGGCG
CGCCACCATGGCAGATCGCCAATCTGGGCGGCGGAAATCGCCCCCTCCCCTCCTCCGACTTGGTTCTGGCCTATCAGGAC
GTGCTATTCGCCCTGCAGCCCGACAACGGTGTCAACAATGGTAGCCCCTCACTGCATGCCCGGCTCCTGGCAGAACTGGA
CATACAAATCGGCGATCGCATCGCCCATATCGGTGCCGGCACCGGTTATTACAGCGCAATTCTCGCAGAGTTGGTCGGCA
CTTCTGGTCATGTCTATGCCGTCGAGATGGATCCCGATCTGGCGGCCCACGCGCAAGCAGCGCTTGCCGAGCGCGCCAAT
GTTAGCGTCATCAACGCCGACGGTAGTCAATGGCCGCAGCAAGAGGTGGATGCGATCTATGTGAATTTCGCCGTCGCGCG
GCCCGCGGAACCCTGGATCGAAAGGCTGCGGCCTGGAGGGCGTCTGGTGCTGCCACTCGGCGTACCGCGCCAGGACCGAC
CGCCGAAAGGCGGCCGTCATGCGTCCCATGGTGCTGCACTTCGTATCGAGCACGGCGAGGGTGGATTTGCGGCGCGTTGG
ATCGGCACAGCCTATTTCGTCTGCGCCAACGGTGGGTTGACGATCGACGATAACGAGATCGAGGCGCTGACCGCCGCCTT
CAGGCGCGGCGGCATCGAGTTCGTCAAAAGCCTGCTCTGGAAGACGGAGCCGCGGATAGGGCGCTGCTGGCATATCGGCG
GCCAATGGGCGCTCTGCTATGACGACGTCTGA

Upstream 100 bases:

>100_bases
GATAGCTTCCGTCACCAGTTCACCTCCTCCATGAATCAATCATTCGGGATAGAAATGGATTTGGAGCCGCAAAGCGCAAA
AAGGGGAACAGGTCAAAGCC

Downstream 100 bases:

>100_bases
GCCATCCGCGCCGGTTCGGCAAAACCGACCACCCATCCCTGCGGAGAACGGGAGTGACGTAGCGCGCCGCGGCACCCAAG
AGTTTGACGTTCTCGGCACC

Product: putative protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MPGDLPMPTTMFTADELAVIRRAYAHQVMATAATTNPRIEAAFATVPREKFLGAPPWQIANLGGGNRPLPSSDLVLAYQD
VLFALQPDNGVNNGSPSLHARLLAELDIQIGDRIAHIGAGTGYYSAILAELVGTSGHVYAVEMDPDLAAHAQAALAERAN
VSVINADGSQWPQQEVDAIYVNFAVARPAEPWIERLRPGGRLVLPLGVPRQDRPPKGGRHASHGAALRIEHGEGGFAARW
IGTAYFVCANGGLTIDDNEIEALTAAFRRGGIEFVKSLLWKTEPRIGRCWHIGGQWALCYDDV

Sequences:

>Translated_303_residues
MPGDLPMPTTMFTADELAVIRRAYAHQVMATAATTNPRIEAAFATVPREKFLGAPPWQIANLGGGNRPLPSSDLVLAYQD
VLFALQPDNGVNNGSPSLHARLLAELDIQIGDRIAHIGAGTGYYSAILAELVGTSGHVYAVEMDPDLAAHAQAALAERAN
VSVINADGSQWPQQEVDAIYVNFAVARPAEPWIERLRPGGRLVLPLGVPRQDRPPKGGRHASHGAALRIEHGEGGFAARW
IGTAYFVCANGGLTIDDNEIEALTAAFRRGGIEFVKSLLWKTEPRIGRCWHIGGQWALCYDDV
>Mature_302_residues
PGDLPMPTTMFTADELAVIRRAYAHQVMATAATTNPRIEAAFATVPREKFLGAPPWQIANLGGGNRPLPSSDLVLAYQDV
LFALQPDNGVNNGSPSLHARLLAELDIQIGDRIAHIGAGTGYYSAILAELVGTSGHVYAVEMDPDLAAHAQAALAERANV
SVINADGSQWPQQEVDAIYVNFAVARPAEPWIERLRPGGRLVLPLGVPRQDRPPKGGRHASHGAALRIEHGEGGFAARWI
GTAYFVCANGGLTIDDNEIEALTAAFRRGGIEFVKSLLWKTEPRIGRCWHIGGQWALCYDDV

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 32612; Mature: 32481

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGDLPMPTTMFTADELAVIRRAYAHQVMATAATTNPRIEAAFATVPREKFLGAPPWQIA
CCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHCCCCCCEEE
NLGGGNRPLPSSDLVLAYQDVLFALQPDNGVNNGSPSLHARLLAELDIQIGDRIAHIGAG
ECCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHCHHHCCEEEEECCC
TGYYSAILAELVGTSGHVYAVEMDPDLAAHAQAALAERANVSVINADGSQWPQQEVDAIY
CHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCCHHHCCEEE
VNFAVARPAEPWIERLRPGGRLVLPLGVPRQDRPPKGGRHASHGAALRIEHGEGGFAARW
EEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEE
IGTAYFVCANGGLTIDDNEIEALTAAFRRGGIEFVKSLLWKTEPRIGRCWHIGGQWALCY
CCEEEEEEECCCEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCEEEEE
DDV
CCC
>Mature Secondary Structure 
PGDLPMPTTMFTADELAVIRRAYAHQVMATAATTNPRIEAAFATVPREKFLGAPPWQIA
CCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHCCCCCCEEE
NLGGGNRPLPSSDLVLAYQDVLFALQPDNGVNNGSPSLHARLLAELDIQIGDRIAHIGAG
ECCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHCHHHCCEEEEECCC
TGYYSAILAELVGTSGHVYAVEMDPDLAAHAQAALAERANVSVINADGSQWPQQEVDAIY
CHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCCHHHCCEEE
VNFAVARPAEPWIERLRPGGRLVLPLGVPRQDRPPKGGRHASHGAALRIEHGEGGFAARW
EEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEE
IGTAYFVCANGGLTIDDNEIEALTAAFRRGGIEFVKSLLWKTEPRIGRCWHIGGQWALCY
CCEEEEEEECCCEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCEEEEE
DDV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA