Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 116249042

GI number: 116249042

Start: 402216

End: 403499

Strand: Reverse

Name: mdeA [H]

Synonym: pRL120373

Alternate gene names: 116249042

Gene position: 403499-402216 (Counterclockwise)

Preceding gene: 116249043

Following gene: 116249035

Centisome position: 46.38

GC content: 62.77

Gene sequence:

>1284_bases
ATGACCGCCCCGCATCCTTCCAAAACCCATATCGGCAATCATGCCCTGCATCCCGAAACGCAGATGCTAAATTACGGCTA
CGATCCGGAGCTCTCGGAAGGGGCGGTCAAGCCGCCGGTATTCCTCACCTCGACCTTCGTGTTCAAGACGGCGGAAGATG
GCCGCGACTTCTTCGATTACGTGTCTGGCCGTCGCGAGCCGCCCGAAGGGAAGGGGGCAGGCCTCGTCTATTCGCGCTTC
AACCATCCAAACAGCGAGATCGTCGAGGACAGGCTCGCCGTCTACGAACGGACGGAAAGCGGCGTGCTGTTTTCATCCGG
CATGGCGGCGATTGCCACCACCCTGCTTGCCTTCGTCCGGCCGGGCGATGCGATCCTGCATTCGCAGCCGCTCTACGGCG
GAACCGAAACATTGCTGGCGAAGACCTTCCTGAACCTCGGCGCCGCCGCCGTCGGATTTGCCGACGGCGTCAGCGAAGGA
TCGGTGCAGAAGGCGGCTGAAGAGGCGATGGCCAAGGGCCGCGTTTCCGTCATCCTGATCGAGACGCCTGCCAATCCGAC
CAACAGTCTGGTCGATGTGGCGATGATCCGACGCGTGGCCGACGCGATCGGTGCAAGACAGGGCCATATGCCGATCATCG
TCTGCGACAACACCCTGCTCGGGCCGGTCTTCCAGCGACCGATCGAGCACGGCGCCGATATCTCGCTCTATTCGCTGACA
AAATATGTCGGTGGTCATTCCGATCTGATCGCCGGCGCCGTTCTTGGCCGCAAGGCGGTGATCAAGCAGATCAAGGCCTT
GCGCGGCGCGATCGGCACCCAGCTCGATCCGCATTCCTGCTGGATGCTCGGCCGTTCGCTGGAAACGCTGCAGCTCCGTA
TGGAACGGGCAAACAGCAACGCGCGGGCCGTCGCCGACTTTCTGCGCGATCACCCGAAGGTCGAGAAGGTTCACTACCTG
CCCTACCACGATCCGGAATCGCCGGCCGGCCGGACCTTCATCGCGCAATGCACCGGCGCCGGCTCGACCTTCTCTTTCGA
CATCCGCGGCGGTCAGCCTGCCTCGTTCAAATTCCTGAACGCGCTGCAGATCTTCAAGCTCGCGGTCAGCCTGGGGGGCA
CGGAATCGCTTGCGAGCCATCCGGCCGCCATGACGCATTCCGGCGTGCCCGCCGATGTCCGTCAGCGCATCGGCGTTCTG
GAATCGACGATCCGTCTTTCGATCGGCATCGAGCATCCGGACGATCTGATCGCCGACCTGGAGATAGCACTGCAGGCTGC
GTGA

Upstream 100 bases:

>100_bases
GACACTCTGGGCGAGACCATGAACTGACCGGGAAGATTGCTCCTCTCCTATGGTTTTGATGTATTCTGCCATTTCGCCAG
TCGCGACACAGGAGACGATG

Downstream 100 bases:

>100_bases
TCGGGGGGAAACGATGCCCGGCGGCGCCGGGCATCAAGTACCACATTATTTGTCGTCGATCGTCAGTTCCGTCTCGTCTG
TGTCCAGCACGAAGACCGCA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFKTAEDGRDFFDYVSGRREPPEGKGAGLVYSRF
NHPNSEIVEDRLAVYERTESGVLFSSGMAAIATTLLAFVRPGDAILHSQPLYGGTETLLAKTFLNLGAAAVGFADGVSEG
SVQKAAEEAMAKGRVSVILIETPANPTNSLVDVAMIRRVADAIGARQGHMPIIVCDNTLLGPVFQRPIEHGADISLYSLT
KYVGGHSDLIAGAVLGRKAVIKQIKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSNARAVADFLRDHPKVEKVHYL
PYHDPESPAGRTFIAQCTGAGSTFSFDIRGGQPASFKFLNALQIFKLAVSLGGTESLASHPAAMTHSGVPADVRQRIGVL
ESTIRLSIGIEHPDDLIADLEIALQAA

Sequences:

>Translated_427_residues
MTAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFKTAEDGRDFFDYVSGRREPPEGKGAGLVYSRF
NHPNSEIVEDRLAVYERTESGVLFSSGMAAIATTLLAFVRPGDAILHSQPLYGGTETLLAKTFLNLGAAAVGFADGVSEG
SVQKAAEEAMAKGRVSVILIETPANPTNSLVDVAMIRRVADAIGARQGHMPIIVCDNTLLGPVFQRPIEHGADISLYSLT
KYVGGHSDLIAGAVLGRKAVIKQIKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSNARAVADFLRDHPKVEKVHYL
PYHDPESPAGRTFIAQCTGAGSTFSFDIRGGQPASFKFLNALQIFKLAVSLGGTESLASHPAAMTHSGVPADVRQRIGVL
ESTIRLSIGIEHPDDLIADLEIALQAA
>Mature_426_residues
TAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFKTAEDGRDFFDYVSGRREPPEGKGAGLVYSRFN
HPNSEIVEDRLAVYERTESGVLFSSGMAAIATTLLAFVRPGDAILHSQPLYGGTETLLAKTFLNLGAAAVGFADGVSEGS
VQKAAEEAMAKGRVSVILIETPANPTNSLVDVAMIRRVADAIGARQGHMPIIVCDNTLLGPVFQRPIEHGADISLYSLTK
YVGGHSDLIAGAVLGRKAVIKQIKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSNARAVADFLRDHPKVEKVHYLP
YHDPESPAGRTFIAQCTGAGSTFSFDIRGGQPASFKFLNALQIFKLAVSLGGTESLASHPAAMTHSGVPADVRQRIGVLE
STIRLSIGIEHPDDLIADLEIALQAA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=418, Percent_Identity=35.1674641148325, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI299473759, Length=418, Percent_Identity=32.5358851674641, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI25453487, Length=418, Percent_Identity=31.5789473684211, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI1790375, Length=405, Percent_Identity=35.0617283950617, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1789383, Length=415, Percent_Identity=26.9879518072289, Blast_Score=135, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17538055, Length=406, Percent_Identity=33.2512315270936, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17560056, Length=422, Percent_Identity=33.6492890995261, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71980708, Length=415, Percent_Identity=32.289156626506, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6319307, Length=403, Percent_Identity=34.4913151364764, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321904, Length=409, Percent_Identity=29.8288508557457, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=28.110599078341, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321254, Length=431, Percent_Identity=26.9141531322506, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=356, Percent_Identity=25.8426966292135, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19922554, Length=413, Percent_Identity=37.046004842615, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45796; Mature: 45665

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFKTAEDGRDFFDY
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHH
VSGRREPPEGKGAGLVYSRFNHPNSEIVEDRLAVYERTESGVLFSSGMAAIATTLLAFVR
HCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHC
PGDAILHSQPLYGGTETLLAKTFLNLGAAAVGFADGVSEGSVQKAAEEAMAKGRVSVILI
CCCHHEECCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEE
ETPANPTNSLVDVAMIRRVADAIGARQGHMPIIVCDNTLLGPVFQRPIEHGADISLYSLT
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEHHHHH
KYVGGHSDLIAGAVLGRKAVIKQIKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHCCCC
ARAVADFLRDHPKVEKVHYLPYHDPESPAGRTFIAQCTGAGSTFSFDIRGGQPASFKFLN
HHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHH
ALQIFKLAVSLGGTESLASHPAAMTHSGVPADVRQRIGVLESTIRLSIGIEHPDDLIADL
HHHHHHHHHHCCCCHHHHCCCCHHCCCCCCHHHHHHHHHHHHEEEEEEECCCCHHHHHHH
EIALQAA
HHHEECC
>Mature Secondary Structure 
TAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFKTAEDGRDFFDY
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHH
VSGRREPPEGKGAGLVYSRFNHPNSEIVEDRLAVYERTESGVLFSSGMAAIATTLLAFVR
HCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHC
PGDAILHSQPLYGGTETLLAKTFLNLGAAAVGFADGVSEGSVQKAAEEAMAKGRVSVILI
CCCHHEECCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEE
ETPANPTNSLVDVAMIRRVADAIGARQGHMPIIVCDNTLLGPVFQRPIEHGADISLYSLT
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEHHHHH
KYVGGHSDLIAGAVLGRKAVIKQIKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHCCCC
ARAVADFLRDHPKVEKVHYLPYHDPESPAGRTFIAQCTGAGSTFSFDIRGGQPASFKFLN
HHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHH
ALQIFKLAVSLGGTESLASHPAAMTHSGVPADVRQRIGVLESTIRLSIGIEHPDDLIADL
HHHHHHHHHHCCCCHHHHCCCCHHCCCCCCHHHHHHHHHHHHEEEEEEECCCCHHHHHHH
EIALQAA
HHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]